自然低温下23个青钱柳家系耐寒性评价

doi:10.12302/j.issn.1000-2006.202208024

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南京林业大学学报（自然科学版） ›› 2024, Vol. 48 ›› Issue (4): 85-92.doi: 10.12302/j.issn.1000-2006.202208024

所属专题：专题报道Ⅱ：乡村振兴视域下药用树种青钱柳培育研究

• 专题报道Ⅱ：乡村振兴视域下药用树种青钱柳培育研究（执行主编李维林、方升佐） • 上一篇下一篇

自然低温下23个青钱柳家系耐寒性评价

张赞培¹(), 谷月营¹, 尚旭岚¹^,², 王纪¹^,³, 方升佐¹^,²^,^*()

1.南京林业大学林草学院,江苏南京 210037
2.南京林业大学,南方现代林业协同创新中心, 江苏南京 210037
3.南京晓庄学院,江苏南京 210037

收稿日期:2022-08-12 修回日期:2023-02-02 出版日期:2024-07-30 发布日期:2024-08-05
通讯作者: *方升佐(fangsz@njfu.edu.cn),教授。
作者简介:
张赞培(zhangzanpei@njfu.edu.cn),博士生。
基金资助:
江苏省重点研发计划(现代农业)重点项目(BE2019388);江苏省研究生科研与实践创新计划项目(KYCX23_1215)

An evaluation on the cold tolerance of twenty-three Cyclocarya paliurus families under natural low temperatures

ZHANG Zanpei¹(), GU Yueying¹, SHANG Xulan¹^,², WANG Ji¹^,³, FANG Shengzuo¹^,²^,^*()

1. College of Forestry and Grassland, Nanjing Forestry University, Nanjing 210037, China
2. Co-Innovation Center for Sustaniable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China
3. Nanjing Xiaozhuang University, Nanjing 210037, China

Received:2022-08-12 Revised:2023-02-02 Online:2024-07-30 Published:2024-08-05

摘要/Abstract

摘要：

【目的】评价青钱柳(Cyclocarya paliurus)不同家系受自然低温胁迫后的耐寒性,筛选出耐寒能力强的家系,为青钱柳的引种、选育和栽培提供参考。【方法】以青钱柳23个家系的1年生枝条为研究材料,在自然低温胁迫后测定其相对电导率(REC),丙二醛(MDA)、可溶性糖(SS)、可溶性蛋白(SP)、淀粉(ST)和游离脯氨酸(Pro)含量,以及过氧化物酶(POD)、超氧化物歧化酶(SOD)活性等指标,并结合主成分分析、聚类分析等方法对不同家系青钱柳的耐寒性进行初步评价。【结果】自然低温胁迫后,23个家系的1年生枝条中相对电导率、过氧化物酶和超氧化物歧化酶活性,以及丙二醛、可溶性糖、可溶性蛋白、淀粉和游离脯氨酸含量等指标存在显著差异。主成分分析发现,4个主成分能够代表各项生理指标72.4%的信息。将各家系的主成分综合得分进行聚类分析,23个家系的耐寒性可分为3大类:第1类综合得分为1.208~1.284,耐寒性较强,仅包含2个家系(SCMC31和ZJTTS2);第2类综合得分为-0.343~0.631,耐寒性一般,包含GZSQ12、GXBS12、ZJFYS6、AHQLF8、HBWF10等17个家系;第3类综合得分为-1.259~-0.745,耐寒性较差,包含GZSQ9、ZJTTS3、SCMC22、SCMC30等4个家系。【结论】自然低温胁迫后,不同家系青钱柳1年生枝条的相关耐寒性生理指标差异显著,综合主成分分析和聚类分析结果,23个青钱柳家系在耐寒性上可以分为3大类。研究结果可为深入研究青钱柳耐寒机理及耐寒青钱柳家系(品种)的筛选提供参考。

关键词: 青钱柳, 家系, 低温胁迫, 主成分分析, 生理指标, 综合评价

Abstract:

【Objective】To provide a theoretical basis for the introduction, selective breeding, and cultivation of Cyclocarya paliurus, the cold resistance of different families of C. paliurus under natural low temperature stress was evaluated.【Method】Using the current branches of C. paliurus from 23 families as materials, the relative electric conductivity (REC), malondialdehyde (MDA) content, peroxidase (POD) activity, superoxide dismutase (SOD) activity, soluble sugar (SS), soluble protein (SP), starch (ST) and free proline (Pro) content were determined after natural low temperature stress. A preliminary evaluation of the cold resistance of C. paliurus families was conducted through a principal component analysis (PCA) and cluster analysis.【Result】After natural low temperature stress, there were significant differences in the REC, MDA, POD and SOD activity, SS, SP, ST and Pro content among the current branches of the 23 families. The PCA found that the four principal components represented 72.4% of the information regarding the various physiological indicators. A cluster analysis showed that the cold resistance of 23 families could be divided into three categories based on the comprehensive score of the PCA for each family. The first category included only two families (SCMC31 and ZJTTS2) with the good cold resistance, and the compositive score ranging from 1.208 to 1.284. The second category indude 17 families (including GZSQ12, GXBS12, ZJFYS6, AHQLF8 and HBWF10) that exhibited moderate cold resistance, with composite scores ranging from -0.343 to 0.631. The third category included four families (GZSQ9, ZJTTS3, SCMC22, and SCMC30) with poor cold tolerance, with composite scores ranging from -1.259 to -0.745.【Conclusion】After natural low temperature stress, significant differences in the measured physiological indices were observed between the current branches of C. paliurus from different families (P < 0.05). Based on the PCA and cluster analysis results, there were different cold resistances among the 23 Cyclocarya paliurus families, which could be divided into three categories. The results not only provide a basis for an in-depth study of the cold tolerate mechanism of C. paliurus, but also for future screening of the cold tolerate genotypes of C. paliurus.

Key words: Cyclocarya paliurus, family, low temperature stress, principal component analysis(PCA), physiological indicators, overall evaluation

中图分类号:

S725
S718

张赞培,谷月营,尚旭岚,等. 自然低温下23个青钱柳家系耐寒性评价[J]. 南京林业大学学报（自然科学版）, 2024, 48(4): 85-92.

ZHANG Zanpei, GU Yueying, SHANG Xulan, WANG Ji, FANG Shengzuo. An evaluation on the cold tolerance of twenty-three Cyclocarya paliurus families under natural low temperatures[J].Journal of Nanjing Forestry University (Natural Science Edition), 2024, 48(4): 85-92.DOI: 10.12302/j.issn.1000-2006.202208024.

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家系 family	可溶性糖含量/(mg·g^-1) soluble sugar content	可溶性蛋白含量/(mg·g^-1) soluble protein content	淀粉含量/(mg·g^-1) starch content	游离脯氨酸含量/(μg·g^-1) free proline content
AHJD1	11.19±0.63 defg	4.56±0.31 f	3.70±1.31 abcdef	128.53±3.35 g
AHQLF3	15.63±1.23 a	2.44±0.16 jk	4.72±1.15 ab	66.13±1.67 k
AHQLF8	11.32±0.72 cdefg	4.70±0.26 f	3.26±0.68 bcdef	175.73±1.62 e
AHQLF16	10.96±0.50 defgh	4.00±0.33 gh	3.76±0.79 abcde	174.53±4.20 e
GXBS12	11.92±0.49 cde	4.15±0.10 g	4.96±1.34 a	93.07±2.72 i
GXJZS1	10.11±1.12 efgh	3.80±0.18 gh	2.77±0.18 def	101.60±1.20 h
GZJH15	9.46±0.15 gh	3.99±0.13 gh	4.21±1.07 abcd	159.87±0.61 f
GZLP4	11.28±0.36 cdefg	5.21±0.24 e	4.13±0.20 abcd	224.40±13.51 c
GZSQ9	12.50±0.55 bcd	3.58±0.15 h	3.12±0.26 cdef	34.53±0.61 n
GZSQ12	13.34±2.35 bc	6.64±0.28 bc	3.79±0.21 abcde	164.40±1.20 f
HBWF10	12.99±2.33 bcd	5.71±0.40 d	3.49±0.44 abcdef	391.60±6.68 b
HBWF15	9.69±1.05 fgh	3.64±0.06 h	4.38±0.08 abc	57.07±2.54 l
SCMC22	11.23±1.31 cdefg	3.14±0.23 i	2.25±0.30 f	190.00±3.42 d
SCMC30	8.94±0.46 h	1.95±0.12 l	3.12±0.96 cdef	158.13±1.89 f
SCMC31	16.01±1.64 a	6.99±0.34 ab	4.42±0.88 abc	413.20±3.67 a
ZJFYS2	14.33±0.86 ab	4.01±0.28 gh	2.45±0.44 ef	162.53±8.09 f
ZJFYS6	11.97±1.15 cde	7.27±0.30 a	3.47±0.27 abcdef	45.87±1.01 m
ZJLWS3	11.74±0.72 cdef	2.15±0.08 kl	4.52±1.05 abc	58.60±1.00 l
ZJMWL4	9.67±0.65 fgh	6.47±0.46 c	3.15±0.42 cdef	70.80±2.40 k
ZJSLG9	12.10±0.35 cde	5.29±0.28 e	3.29±0.77 bcdef	36.00±0.40 n
ZJTTS2	15.96±1.61 a	6.59±0.31 bc	4.63±0.50 abc	83.73±2.20 j
ZJTTS3	10.97±0.43 defgh	2.48±0.12 jk	2.61±0.54 ef	33.87±0.23 n
ZJWC1	12.30±0.54 cd	2.68±0.13 j	4.17±1.23 abcd	134.60±1.00 g

主成分 principal component	特征根 eigenvalue	贡献率/% contribution rate	累积贡献率/% cumulative contribution rate
1	1.703	21.292	21.292
2	1.542	19.274	40.566
3	1.420	17.746	58.312
4	1.127	14.086	72.398

生理指标 physiological indicator	主成分principal component
生理指标 physiological indicator	1	2	3	4
可溶性蛋白含量 soluble protein content	0.770	0.067	0.028	0.417
相对电导率 relative electric conductivity	-0.725	-0.323	0.195	0.092
游离脯氨酸含量 free proline content	0.627	0.024	-0.541	-0.163
淀粉含量 starch content	0.108	0.747	0.366	-0.024
丙二醛含量 malondialdehyde content	0.033	0.735	-0.453	0.027
可溶性糖含量 soluble sugar content	0.385	0.574	-0.169	-0.085
过氧化物酶活性 peroxidase activity	-0.169	-0.056	0.846	-0.104
超氧化物歧化酶活性 superoxide dismutase activity	0.034	-0.053	-0.066	0.948

自然低温下23个青钱柳家系耐寒性评价

An evaluation on the cold tolerance of twenty-three Cyclocarya paliurus families under natural low temperatures

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