夏季淹水胁迫对北美枫香苗木叶色及光合荧光特性的影响

doi:10.12302/j.issn.1000-2006.201909045

国家林草科技领军期刊
中国精品科技期刊
中国高校百佳科技期刊
江苏省新闻出版政府奖期刊奖
RCCSE林学权威期刊（A+）
CSCD核心期刊
Scopus数据库收录期刊
中文核心期刊
SCD核心期刊

作者加群：102861116

微信公众号：南京林业大学学报

高级检索

南京林业大学学报（自然科学版） ›› 2021, Vol. 45 ›› Issue (2): 69-76.doi: 10.12302/j.issn.1000-2006.201909045

夏季淹水胁迫对北美枫香苗木叶色及光合荧光特性的影响

仲磊¹^,²(), 张焕朝¹^,^*(), 范俊俊³, 张丹丹⁴, 江皓¹, 张往祥¹

1.南京林业大学林学院,南方现代林业协同创新中心,江苏南京 210037
2.江苏省林木种苗管理站,江苏南京 210036
3.金陵科技学院园艺园林学院,江苏南京 211169
4.金埔园林股份有限公司,江苏南京 211100

收稿日期:2019-09-23 接受日期:2020-04-08 出版日期:2021-03-30 发布日期:2021-04-09
通讯作者: 张焕朝
基金资助:
国家重点研发计划(2015BAD07B01)

Effects of flooding stress on leaf color and photosynthetic fluorescence characteristics of Liquidambar styraciflua cutting seedlings in summer

ZHONG Lei¹^,²(), ZHANG Huanchao¹^,^*(), FAN Junjun³, ZHANG Dandan⁴, JIANG Hao¹, ZHANG Wangxiang¹

1. Co-Innovation Center for the Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China
2. Jiangsu Province Forest Seedling Management Station, Nanjing 210036, China
3. College of Horticulture, Jinling Institute of Technology, Nanjing 211169, China
4. Jinpu Landscape Co., Ltd., Nanjing 211100, China

Received:2019-09-23 Accepted:2020-04-08 Online:2021-03-30 Published:2021-04-09
Contact: ZHANG Huanchao

摘要/Abstract

摘要：

【目的】评价北美枫香(Liquidambar styraciflua)耐涝性,并从叶色与色素、光合荧光等生理指标间的协同性分析其耐涝机理,为沿海和涝害地区耐水湿观赏树种的筛选提供参考。【方法】模拟中国南方洪水季节性发生的特点,在夏季高温期间,对北美枫香1年生扦插苗进行60 d的户外盆栽淹水处理,分析其在淹水过程中生长、叶色(色彩参数与色素比例)及光合特征[净光合速率(P_n)、蒸腾速率(T_r)、气孔导度(G_s)和胞间CO₂浓度(C_i)]、叶绿素荧光动力学特性[单位叶面积反应中心数(N_RC/CSm)单位叶面积热耗散的能量(E_DIo/CSm)、PSⅡ捕获能量从Q_A传递到Q_B的效率(ψ_O)、电子传递的量子比率(φ_Eo)、受体侧末端电子受体的量子产额(φ_Ro)、综合性能指数(I_total)]的动态变化趋势,并根据各指标峰谷值出现时间分析指标间的协同性。【结果】①淹水持续60 d并未对北美枫香的生长产生抑制作用,苗木的成活率能达到90%以上。②叶色参数结果显示,随着淹水时间的延长,色调角h值呈现显著下降趋势,40 d后,叶片由绿色相域转变为红色相域(h<90°)。③叶片色素相对含量比值结果显示,随着淹水时间的延长,处理的叶片叶绿素与花青素及类胡萝卜素相对含量的比值[Chl/(Anth+Car)]呈现下降趋势。20 d后,处理Chl/(Anth+Car)的值显著低于对照(CK),在40 d后,Chl/(Anth+Car)的值小于1(胡萝卜素和花青素相对含量占据主要地位),这与叶片外观色彩参数变化表现出同步性。④处理与CK叶片光合气体交换参数变化趋势整体相似,P_n与C_i均呈现下降—上升—下降趋势,T_r与G_s均呈现下降趋势;在淹水初期(0~20 d)处理与CK各光合参数值差异不显著,但在淹水中后期(20 d后),处理各参数值显著低于CK,且二者差异整体呈现增大趋势。⑤光响应曲线特征表明,处理在各光强下的P_n显著低于CK,且随着淹水时间的延长,处理与CK的P_n差值越大,表现出较低的光能利用率。⑥叶绿素荧光动力学参数表明,淹水30 d后,叶片反应中心数目(N_RC/CSm)和光系统PSⅡ向PSⅠ的电子传递能力(Ψ_o、$φ_{Ro}$和$φ_{Ro}$)均显著下降,叶片的热耗散($E_{\text{DIo/CS m}}$)显著上升,最终导致PSⅡ综合性能指数显著下降。【结论】北美枫香具有较强的短期耐水淹能力,尤其在淹水20 d内,各指标均优于CK;北美枫香主要通过增加叶片热耗散以及调节光合色素含量权重来减少激发能和过剩激发能产生的伤害,从而降低涝害程度;净光合速率、叶片色素比值及荧光参数之间存在协同性,各参数拐点出现的时间呈节律性变化,分别为20、30 及40 d,其中光合参数反应最为灵敏。

关键词: 淹水胁迫, 北美枫香, 色彩空间, 光合特征, 叶绿素荧光, 协同性, 夏季

Abstract:

【Objective】The purpose of this experiment is to estimate the flooding resistance of Liquidambar styraciflua and to analyze the flooding resistance mechanism, combined with the synergy of leaf color, leaf pigments and photosynthetic fluorescence characteristics.【Method】The dynamic trends of growth, leaf color (color parameters and pigment ratio), photosynthetic characteristics [net photosynthetic rate (P_n), transpiration rate (T_r), stomatal conductance (G_s), intercellular CO₂ concentration (C_i)]; and chlorophyll dynamic fluorescence characteristics [leaf reaction center number (N_RC/CSm), efficiency by trapped exciton (Ψ_o), quantum ratio for electron transfer (φ_Ro), quantum yield of electron acceptor at the end of acceptor side (φ_Eo), dissipation (E_DIo/CSm), and performance index (I_total)] of one-year-old cuttings of L. styraciflua were analyzed under 60 days of flooding treatment outdoors in summer at high temperatures. 【Result】①The growth of L. styraciflua was not inhibited by flooding treatment after 60 days, and the survival rate of cuttings was still up to 90%. ② The leaf color parameter (h) decreased significantly with prolongation of flooding. After 40 days, the color of the leaves changed from green to red (h< 90°). ③ The relative content ratio of chlorophyll to anthocyanin and carotenoid [Chl/(Anth+Car)] showed a significant decreasing trend after 20 days. After 40 days, the ratio of pigments was less than 1. The change in pigments was synchronized with that of the leaf color parameters. ④ The trend of photosynthetic gas exchange parameters of treated cuttings was similar to that of the control. Both P_n and C_i showed downward-upward-downward trend, while T_r and G_s showed downward trend. In the early stage of flooding (0-20 days), there was no significant difference between the treatment and control groups, but in the late stage of flooding (20 days later), the values of each parameter were significantly lower than that of the control, and the difference between them increased. ⑤ The characteristics of the light response curve showed that the P_n of the treatment was significantly lower than that of the control, and with flooding prolongation, the difference in P_n between the treatment and control was larger than that of the control, which indicated that the light utilization rate of the treatment was lower. ⑥ With prolonged flooding (after 30 days of flooding), the number of leaf reaction centers (N_RC/CSm) and the energy of electronic transmission from PSII to PSI (Ψ_o, φ_Ro and φ_Eo) were significantly decreased, and the E_DIo/CSm was significantly increased, which resulted in a decrease in the performance index (I_total). 【Conclusion】The L. styraciflua has strong short-term flooding tolerance. All indexes of treatments were better than those of the control in the first 20 days. This was because of the reduction in excitation energy generation and excess excitation energy damage by increasing the heat dissipation of leaves, thereby adjusting the weight of the photosynthetic pigment content. There was synergism among net photosynthetic rate, leaf pigment ratio and fluorescence parameters, and the time of inflection of each parameter changed rhythmically, that is, 20, 30 and 40 days, respectively. The photosynthetic parameter response was the most sensitive.

Key words: flooding stress, Liquidambar styraciflua, color space, characterization of photosynthesis, chlorophyll fluorescence, synergy, summer

中图分类号:

S718
Q945

仲磊,张焕朝,范俊俊,等. 夏季淹水胁迫对北美枫香苗木叶色及光合荧光特性的影响[J]. 南京林业大学学报（自然科学版）, 2021, 45(2): 69-76.

ZHONG Lei, ZHANG Huanchao, FAN Junjun, ZHANG Dandan, JIANG Hao, ZHANG Wangxiang. Effects of flooding stress on leaf color and photosynthetic fluorescence characteristics of Liquidambar styraciflua cutting seedlings in summer[J].Journal of Nanjing Forestry University (Natural Science Edition), 2021, 45(2): 69-76.DOI: 10.12302/j.issn.1000-2006.201909045.

图/表 5

图1

图2

图3

图4

图5

参考文献 31

[1]	顾佳清, 张智奇, 周音, 等. 树种耐水湿筛选研究综述[J]. 上海农业学报, 2004,20(4):66-69.
	GU J Q, ZHANG Z Q, ZHOU Y, et al. Review on screening of waterlogging tolerant trees[J]. Acta Agric Shanghai, 2004,20(4):66-69. DOI: 10.3969/j.issn.1000-3924.2004.04.019.
[2]	HASHIGUCHI A, SAKATA K, KOMATSU S. Proteome analysis of early-stage soybean seedlings under flooding stress.[J]. J Proteome Res, 2009,8(4):2058-2069. DOI: 10.1021/pr801051m.
[3]	张往祥, 张晓燕, 曹福亮, 等. 涝渍胁迫下3个树种幼苗生理特性的响应[J]. 南京林业大学学报(自然科学版), 2011,35(5):11-15.
	ZHANG W X, ZHANG X Y, CAO F L, et al. Effects of flooding stress on the physiological adaption metabolism of three tree species[J]. J Nanjing For Univ(Nat Sci Ed), 2011,35(5):11-15. DOI: 10.3969/j.issn.1000-2006.2011.05.003.
[4]	MUSTAFA G, SAKATA K, KOMATSU S. Proteomic analysis of soybean root exposed to varying sizes of silver nanoparticles under flooding stress[J]. J Proteom, 2016,148:113-125. DOI: 10.1016/j.jprot.2016.07.027.
[5]	BAILEY-SERRES J, VOESENEK L A C J. Flooding stress: acclimations and genetic diversity[J]. Annual Review of Plant Bio, 2008,59:313. DOI: 10.1146/annurev.arplant.59.032607.092752.
[6]	丁红, 张智猛, 戴良香, 等. 水分胁迫和氮肥对花生根系形态发育及叶片生理活性的影响[J]. 应用生态学报, 2015,26(2):450-456.
	DING H, ZHANG Z M, DAI L X, et al. Effects of water stress and nitrogen fertilization on peanut root morphological development and leaf physiological activities[J]. Chin J Appl Ecol, 2015,26(2):450-456. DOI: 10.13287/j.1001-9332.20141223.002.
[7]	俞继红. 3种彩叶植物的光合特性比较[J]. 西北林学院学报, 2014,29(4):21-25.
	YU J H. A comparison of photosynthetic characteristics between three color-leafed plants[J]. J Northwest For Univ, 2014,29(4):21-25. DOI: 10.3969/j.issn.1001-7461.2014.04.04.
[8]	郭彩霞, 陈法志, 童俊, 等. 几种彩叶植物的耐热抗寒性研究[J]. 黑龙江农业科学, 2010(10):77-79.
	GUO C X, CHEN F Z, TONG J, et al. Study on heat resistance and cold resistance of several color-leafed plants[J]. Heilongjiang Agric Sci, 2010(10):77-79. DOI: 10.3969/j.issn.1002-2767.2010.10.026.
[9]	于永根, 蔡开锋, 薛文虎, 等. 北美枫香引种栽培试验研究[J]. 河南林业科技, 2012,32(2):8-10.
	YU Y G, CAI K F, XUE W H, et al. Study on introduction and cultivation of Liquidambar styraciflua[J]. J Henan For Sci Technol, 2012,32(2):8-10. DOI: 10.3969/j.issn.1003-2630.2012.02.00.
[10]	苏梦云. 美国枫香茎段组织培养与植株再生[J]. 林业科学研究, 2005,18(1):98-101.
	SU M Y. Tissue culture of stem segments and plantlet regenation of Liquidambar styraciflua[J]. For Res, 2005,18(1):98-101. DOI: 10.3321/j.issn:1001-1498.2005.01.021.
[11]	BYAMUKAMA R, JORDHEIM M, KIREMIRE B, et al. Anthocyanins from flowers of Hippeastrum cultivars[J]. Sci Hortic, 2006,109(3):262-266. DOI: 10.1016/j.scienta.2006.05.007.
[12]	WHITE S A, SCOGGINS H L. Fertilizer Concentration affects growth response and leaf color of Tradescantia virginiana L[J]. J plant nutr, 2005,28(10):1767-1783. DOI: 10.1080/01904160500250896.
[13]	MERZLYAK M N, SOLOVCHENKO A E, GITELSON A A. Reflectance spectral features and non-destructive estimation of chlorophyll, carotenoid and anthocyanin content in apple fruit[J]. Postharvest Biol Technol, 2003,27(2):197-211. DOI: 10.1016/S0925-5214(02)00066-2.
[14]	YE Z P, SUGGETT D J, ROBAKOWSKI P, et al. A mechanistic model for the photosynjournal-light response based on the photosynthetic electron transport of photosystem II in C₃ and C₄ species[J]. New Phytol, 2013,199(1), 110-120. DOI: 10.1111/nph.12242.
[15]	JACKSON M B, COLMER T D. Response and adaptation by plants to flooding stress[J]. Ann Bot, 2005,96:501-505. DOI: 10.1093/aob/mci205.
[16]	YAMAMOTO F, SAKATA T, TERAZAWA K. Physiological, morphological and anatomical responses of Fraxinus mandshurica seedlings to flooding[J]. Tree Physiol, 1995,15(11):713-719. DOI: 10.1093/treephys/15.11.713.
[17]	PARELLE J, BRENDEL O, BODÉNÈS C, et al. Differences in morphological and physiological responses to water-logging between two sympatric oak species (Quercus petraea (Matt.) Liebl. Quercus robur L.)[J]. Ann For Sci, 2006,63(8):849-859. DOI: 10.1051/forest:2006068.
[18]	聂庆娟, 史宝胜, 孟朝, 等. 不同叶色红栌叶片中色素含量、酶活性及内含物差异的研究[J]. 植物研究, 2008,28(5):599-602.
	NIE Q J, SHI B S, MENG C, et al. The enzyme activities, pigment and inclusion contents in different leaves color of Cotinus coggygria ‘Royal Purple’ in autumn[J]. Bull Bot Res, 2008,28(5):599-602. DOI: 10.7525/j.issn.1673-5102.2008.05.020.
[19]	张敏, 黄利斌, 周鹏, 等. 榉树秋季转色期叶色变化的生理生化[J]. 林业科学, 2015,51(8):44-51.
	ZHANG M, HUANG L B, ZHOU P, et al. Physiological and biochemical changes in Zelkova serrata leaves during leaf color transformation in autumn[J]. Sci Silvae Sin, 2015,51(8):44-51. DOI: 10.11707/j.1001-7488.20150806.
[20]	苏金, 黄婧, 周鹏, 等. 黄金枸骨变色期的叶色与叶片光合色素含量关系研究[J]. 江苏林业科技, 2019,46(4):33-36.
	SU J, HUANG J, ZHOU P, et al. Leaf color research during discoloration period of Ilex × attenuata ‘Sunny Foster’[J]. Journal of Jiangsu Forestry Science & Technology, 2019,46(4):33-36. DOI: 10.3969/j.issn.1001-7380.2019.04.007.
[21]	STEYN W J, WAND S J E, HOLCROFT D M, et al. Anthocyanins in vegetative tissues: a proposed unified function in photoprotection[J]. New Phytolt, 2002,155(3):349-361. DOI: 10.1046/j.1469-8137.2002.00482.x.
[22]	ZHANG L T, ZHANG Z S, GAO H Y, et al. Mitochondrial alternative oxidase pathway protects plants against photoinhibition by alleviating inhibition of the repair of photodamaged PSII through preventing formation of reactive oxygen species in Rumex K-1 leaves[J]. Physiol Plant, 2011,143(4):396-407. DOI: 10.1111/j.1399-3054.2011.01514.x.
[23]	DEMMIG-ADAMS B, ADAMS W W. The role of xanthophyll cycle carotenoids in the protection of photosynjournal[J]. Trends Plant Sci, 1996,1(1):21-26. DOI: 10.1016/S1360-1385(96)80019-7.
[24]	衣宁, 赵文倩, 刘倩, 等. 油松新生叶与老叶光合功能的比较[J]. 林业科技, 2014,39(6):10-14.
	YI N, ZHAO W Q, LIU Q, et al. Comparation on photosynthetic capacity of new leaves and older leaves of Pinus tabuliformis[J]. For Sci Technol, 2014,39(6):10-14. DOI: 10.3969/j.issn.1001-9499.2014.06.003.
[25]	杨小鑫, 吕运舟, 董筱昀, 等. ‘金焰彩栾’与黄山栾树光合特性比较[J]. 南京林业大学学报(自然科学版), 2016,40(4):74-80.
	YANG X X, LV Y Z, DONG Y Y, et al. Photosynthetic characteristics of Koelreuteria bipinnata var. integrifoliola and its natural yellow mutant ‘Jinyan’[J]. J Nanjing For Univ(Nat Sci Ed), 2016,40(4):74-80. DOI: 10.3969/j.issn.1000-2006.2016.04.012.
[26]	董贞芬, 门宇恒, 邹秋滢, 等. 叶绿素荧光成像在植物生长环境胁迫中的应用[J]. 沈阳农业大学学报, 2019,50(2):250-256.
	DONG Z F, MEN Y H, ZOU Q Y, et al. Application of chlorophyll fluorescence imaging in plant growth environment stress[J]. J Shenyang Agric Univ, 2019,50(2):250-256. DOI: 10.3969/j.issn.1000-1700.2019.02.018.
[27]	MALIK A I, COLMER T D, LAMBERS H, et al. Changes in physiological and morphological traits of roots and shoots of wheat in response to different depths of waterlogging[J]. Australian J Plant Physiol, 2001,28(11):1121-1131. DOI: 10.1016/S0014-5793(97)00086-0.
[28]	刘晓军, 唐晓波, 李春华. 不同绿茶品种秋季叶绿素与光合效率比较及相关性研究[J]. 西南农业学报, 2008,21(4):975-978.
	LIU X J, TANG X B, LI C H. Comparative and correlation study on chlorophyll and photosynjournal efficiency of the different green tea at autumn[J]. Southwest China J AgricSci, 2008,21(4):975-978. DOI: 10.3969/j.issn.1001-4829.2008.04.020.
[29]	ZUSHI K, KAJIWARA S, MATSUZOE N. Chlorophyll a fluorescence OJIP transient as a tool to characterize and evaluate response to heat and chilling stress in tomato leaf and fruit[J]. Sci Hortic, 2012,148:39-46. DOI: 10.1016/j.scienta.2012.09.022.
[30]	朱向涛, 金松恒, 哀建国, 等. 淹水胁迫下江南牡丹生长及光合特性研究[J]. 广西植物, 2016,36(8):956-962.
	ZHU X T, JIN S H, AI J G, et al. Effects of flooding stress on growth and photosynjournal of Paeonia suffruticosa[J]. Guihaia, 2016,36(8):956-962. DOI: 10.11931/guihaia.gxzw201501032.
[31]	杨小苗, 吴新亮, 刘玉凤, 等. 一个番茄EMS叶色黄化突变体的叶绿素含量及光合作用[J]. 应用生态学报, 2018,29(6):1983-1989.
	YANG X M, WU X L, LIU Y F, et al. Analysis of chlorophyll and photosynjournal of a tomato chlorophyll-deficient mutant induced by EMS[J]. Chin J Appl Ecol, 2018,29(6):1983-1989. DOI: 10.13287/j.1001-9332.201806.021.

夏季淹水胁迫对北美枫香苗木叶色及光合荧光特性的影响

Effects of flooding stress on leaf color and photosynthetic fluorescence characteristics of Liquidambar styraciflua cutting seedlings in summer

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 5

参考文献 31

相关文章 15

编辑推荐

Metrics

本文评价

作者

读者

审稿

[1]	田梦阳, 朱树林, 窦全琴, 季艳红. 薄壳山核桃-茶间作对‘安吉白茶’速生期光合特性的影响[J]. 南京林业大学学报（自然科学版）, 2024, 48(2): 86-96.
[2]	魏静, 谭星, 王昌盛, 闫瑞, 李林珂, 宁月, 刘芸. 引种美国红枫在两种紫色土区的生长和光合特性比较[J]. 南京林业大学学报（自然科学版）, 2024, 48(1): 97-105.
[3]	贾瑞瑞, 祝艳艳, 杨秀莲, 付钰, 岳远征, 王良桂. 不同砧木对楸树嫁接苗生长及光合特性的影响[J]. 南京林业大学学报（自然科学版）, 2023, 47(5): 97-106.
[4]	田梦阳, 窦全琴, 谢寅峰, 汤文华, 季艳红. 4个薄壳山核桃品种的光合特性研究[J]. 南京林业大学学报（自然科学版）, 2022, 46(5): 67-74.
[5]	黄小辉, 吴焦焦, 王玉书, 冯大兰, 孙向阳. 不同供氮水平的核桃幼苗生长及叶绿素荧光特性[J]. 南京林业大学学报（自然科学版）, 2022, 46(2): 119-126.
[6]	季艳红, 潘平平, 窦全琴, 谢寅峰. 不同泥炭替代基质对薄壳山核桃幼苗生长及叶绿素荧光特性的影响[J]. 南京林业大学学报（自然科学版）, 2022, 46(1): 145-155.
[7]	袁婷婷, 路远峰, 谢寅峰, 马迎莉, 吴桐, 倪震. 硼钼铜微肥配施对太子参光合特性的影响[J]. 南京林业大学学报（自然科学版）, 2021, 45(4): 130-136.
[8]	圣倩倩, 戴安琪, 宋敏, 唐睿, 祝遵凌. NO₂胁迫下两种鹅耳枥的光合生理特性变化[J]. 南京林业大学学报（自然科学版）, 2021, 45(2): 10-16.
[9]	汤文华, 窦全琴, 潘平平, 季艳红, 谢寅峰. 不同薄壳山核桃品种光合特性研究[J]. 南京林业大学学报（自然科学版）, 2020, 44(3): 81-88.
[10]	李晓锐, 周樊, 冯刚, 郑小琴, 李永荣, 彭方仁. 砧木对薄壳山核桃嫁接苗光合及荧光特性的影响[J]. 南京林业大学学报（自然科学版）, 2020, 44(2): 84-90.
[11]	徐清, 闭鸿雁, 崔光帅, 郭晓荣, 周睿, 苏文华, 欧阳志勤, 张光飞. 珍稀濒危植物毛果木莲幼苗光合特性及对遮阴处理的响应[J]. 南京林业大学学报（自然科学版）, 2019, 43(6): 46-52.
[12]	马迎莉,高雨,袁婷婷,代丽,张雨峰,谢寅峰. 重金属铬胁迫对髯毛箬竹光合特性的影响[J]. 南京林业大学学报（自然科学版）, 2019, 43(01): 54-60.
[13]	吕东林,林琳,郭译文,韩锐,姜静. 紫雨桦耐盐性及花色苷合成相关基因的表达特性[J]. 南京林业大学学报（自然科学版）, 2018, 42(02): 25-32.
[14]	王茜,王成2,杜万光,许超,郭珺琪. 福州旗山夏季毛竹林生态保健功能研究[J]. 南京林业大学学报（自然科学版）, 2018, 42(02): 120-126.
[15]	张虎,曹福亮,范俊俊,张往祥. 淹水胁迫对湖北海棠生长及叶绿素荧光动力学的影响[J]. 南京林业大学学报（自然科学版）, 2018, 42(01): 35-40.