毛竹及其变种叶片化学计量与养分重吸收效率

郭雯, 漆良华, 雷刚, 胡璇, 张建, 舒琪, 商泽安

南京林业大学学报(自然科学版) ›› 2021, Vol. 45 ›› Issue (1) : 79-85.

PDF(1557 KB)
PDF(1557 KB)
南京林业大学学报(自然科学版) ›› 2021, Vol. 45 ›› Issue (1) : 79-85. DOI: 10.12302/j.issn.1000-2006.201910034
研究论文

毛竹及其变种叶片化学计量与养分重吸收效率

作者信息 +

Leaf stoichiometry and nutrient reabsorption efficiency of Phyllostachys edulis and its varieties

Author information +
文章历史 +

摘要

【目的】评价毛竹(Phyllostachys edulis)及其变种叶片养分重吸收效率,旨在揭示主要养分元素的生态适应机制,以期为毛竹及其变种的可持续经营提供科学依据。【方法】以毛竹及其变种[黄槽毛竹(P. edulis cv. Luteosulcata)、花毛竹(P. edulis cv. Tao Kiang)、厚壁毛竹(P. edulis cv. Pachyloen)、金丝毛竹(P. edulis cv. Gracilis)]为研究对象,分析不同竹龄(1、3、5 a)叶片化学计量特征与养分重吸收效率。【结果】毛竹及其变种C、N 含量差异较小,P含量波动性较大。不同竹种相同竹龄立竹间成熟叶和凋落叶C、N、P含量差异性较大,1年生竹种叶片养分含量较高,随着竹龄增加,竹种适应能力逐渐下降。毛竹及其变种相同年龄立竹间、同一变种不同年龄立竹间叶片化学计量比存在一定差异性。1、3、5年生花毛竹叶片N、P重吸收效率较高,年龄对除厚壁毛竹外的其他毛竹及其变种叶片N重吸收效率影响呈现先升后降趋势,而不同年龄毛竹及其变种叶片P重吸收效率波动性较大。【结论】研究区毛竹及其变种生长受P元素的限制,毛竹及其变种随竹龄的增加适应能力有所变化,花毛竹适应性较强,厚壁毛竹则对土壤的依赖性较大。

Abstract

【Objective】 The objective of this work was to evaluate the nutrient reabsorption efficiency of Phyllostachys edulis and its varieties, and to identify the ecological adaptation mechanism of the main nutrient elements. This will provide a scientific basis for the sustainable management of P. edulis and its varieties. 【Method】In this study, the leaf stoichiometric characteristics and nutrient reabsorption efficiency of P. edulis and its varieties (P. edulis cv. Luteosulcata, P. edulis cv. Tao Kiang, P. edulis cv. Pachyloen, and P. edulis cv. Gracilis) were analyzed at different ages (1, 3, 5 a). 【Result】 The content of carbon and nitrogen in the leaves of P. edulis and its varieties varied slightly, while the content of phosphorus fluctuated greatly. The contents of C, N and P in the leaves of different bamboo species at the same age were significantly different. The nutrient content in leaves of 1-year bamboo was relatively high. With an increase in age, the adaptability of bamboo gradually decreased. There were some differences in the leaf stoichiometric ratio between P. edulis and its varieties at the same age and between the same variety at different ages. The reabsorption efficiency of N and P in the leaves of P. edulis at different ages was higher. The effect of age on the N reabsorption efficiency of leaves of P. edulis and its varieties increased at first and then decreased, but the P reabsorption efficiency of bamboo leaves fluctuated greatly at different ages. 【Conclusion】 The growth of P. edulis and its varieties was limited by the P element, and the adaptability changed with an increase in age for P. edulis and its varieties. P. edulis cv. Tao Kiang showed strong adaptability, while P. edulis cv. Pachyloen had the opposite trait.

关键词

毛竹变种 / C、N、P生态化学计量 / 成熟叶 / 凋落叶 / 重吸收效率

Key words

Phyllostachys edulis varieties / C/N/P ecological stoichiometry / mature leaf / withered leaf / reabsorption efficiency

引用本文

导出引用
郭雯, 漆良华, 雷刚, . 毛竹及其变种叶片化学计量与养分重吸收效率[J]. 南京林业大学学报(自然科学版). 2021, 45(1): 79-85 https://doi.org/10.12302/j.issn.1000-2006.201910034
GUO Wen, QI Lianghua, LEI Gang, et al. Leaf stoichiometry and nutrient reabsorption efficiency of Phyllostachys edulis and its varieties[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2021, 45(1): 79-85 https://doi.org/10.12302/j.issn.1000-2006.201910034
中图分类号: S718   

参考文献

[1]
李明军, 喻理飞, 杜明凤, 等. 不同林龄杉木人工林植物-凋落叶-土壤C、N、P化学计量特征及互作关系[J]. 生态学报, 2018,38(21):7772-7781.
LI M J, YU L F, DU M F, et al. C,N,and P stoichiometry and their interaction with plants,litter,and soil in a Cunninghamia lanceolata plantation with different ages[J]. Acta Ecol Sin, 2018,38(21):7772-7781. DOI: 10.5846/stxb201708221509.
[2]
JI M F, WU S F, ZHANG X W. C,N,and P Ecological stoichiometric characteristics of two dominant mosses in Baotianman Nature Reserve[J]. Asian Agric Res, 2017,9(8):40-44. DOI: 10.19601/j.cnki.issn1943-9903.2017.08.011.
[3]
贺金生, 韩兴国. 生态化学计量学:探索从个体到生态系统的统一化理论[J]. 植物生态学报, 2010,34(1):2-6.
HE J S, HAN X G. Ecological stoichiometry: searching for unifying principles from individuals to ecosystems[J]. Chin J Plant Ecol, 2010,34(1):2-6. DOI: 10.3773/j.issn.1005-264x.2010.01.002.
[4]
于贵瑞, 李轩然, 赵宁, 等. 生态化学计量学在陆地生态系统碳-氮-水耦合循环理论体系中作用初探[J]. 第四纪研究, 2014,34(4):881-890.
YU G R, LI X R, ZHAO N, et al. Theoretical linkage betwenn ecological stoichiometry with the coupled cycle of carbon,nitrogen and water in terrestrial ecosystems[J]. Quat Sci, 2014,34(4):881-890. DOI: 10.3969/j.issn.1001-7410.2014.04.21.
[5]
曾德慧, 陈广生, 陈伏生, 等. 不同林龄樟子松叶片养分含量及其再吸收效率[J]. 林业科学, 2005,41(5):21-27.
ZENG D H, CHEN G S, CHEN F S, et al. Foliar nutrients and their resorption efficiencies in four Pinus sylvestris var.mongolica plantations of different ages on sandy soil[J]. Sci Silvae Sin, 2005,41(5):21-27. DOI: 10.3321/j.issn:1001-7488.2005.05.004.
[6]
WANG M, LIU G H, JIN T T, et al. Age-related changes of leaf traits and stoichiometry in an alpine shrub (Rhododendron agglutinatum) along altitudinal gradient[J]. J Mt Sci, 2017,14(1):106-118. DOI: 10.1007/s11629-016-4096-y.
[7]
SANTA REGINA I, LEONARDI S, RAPP M. Foliar nutrient dynamics and nutrient-use efficiency in Castanea sativa coppice stands of southern Europe[J]. Forestry (Lond), 2001,74(1):1-10. DOI: 10.1093/forestry/74.1.1.
[8]
AERTS R. Nutrient use efficiency in evergreen and deciduous species from heathlands[J]. Oecologia, 1990,84(3):391-397. DOI: 10.1007/BF00329765.
The nutrient (N, P) use efficiency (NUE: g g(-1) nutrient), measured for the entire plant, of field populations of the evergreen shrubs Erica tetralix (in a wet heathland) and Calluna vulgaris (in a dry heathland) and the deciduous grass Molinia caerulea (both in a wet and a dry heathland) was compared. Erica and Calluna are crowded out by Molinia when nutrient availability increases. NUE was measured as the product of the mean residence time of a unit of nutrient in the population (MRT: yr) and nutrient productivity (A: annual productivity per unit of nutrient in the population, g g(-1) nutrient yr(-1). It was hypothesized that 1) in low-nutrient habitats selection is on features leading to a high MRT, whereas in high-nutrient habitats selection is on features leading to a high A; and that 2) due to evolutionary trade-offs plants cannot combine genotypically determined features which maximize both components of NUE.Both total productivity and litter production of the Molinia populations exceeded that of both evergreens about three-fold. Nitrogen and phosphorus resorption from senescing shoots was much lower in the evergreens compared with Molinia. In a split-root experiment no nutrient resorption from senescing roots was observed. Nutrient concentrations in the litter were equal for all species, except for litter P-concentration of Molinia at the wet site. Both Erica and Calluna had a long mean residence time of both nitrogen and phosphorus and a low nitrogen and phosphorus productivity. The Molinia populations showed a shorter mean residence time of N and P and a higher N- and P-productivity. These patterns resulted in an equal nitrogen use efficiency and an almost equal phosphorus use efficiency for the species under study. However, when only aboveground NUE was considered the Molinia populations had a much higher NUE than the evergreens.The results are consistent with the hypotheses. Thus, the low potential growth rate of species from low-nutrient habitats is probably the consequence of their nutrient conserving strategy rather than a feature on which direct selection takes place in these habitats.
[9]
CAO Y, CHEN Y M. Coupling of plant and soil C∶N∶P stoichiometry in black locust (Robinia pseudoacacia) plantations on the Loess Plateau,China[J]. Trees, 2017,31(5):1559-1570. DOI: 10.1007/s00468-017-1569-8.
[10]
ZHAO N, HE N P, WANG Q F, et al. The altitudinal patterns of leaf C∶N∶P stoichiometry are regulated by plant growth form,climate and soil on Changbai Mountain,China[J]. PLoS One, 2014,9(4):e95196. DOI: 10.1371/journal.pone.0095196.
Understanding the geographic patterns and potential drivers of leaf stoichiometry is critical for modelling the nutrient fluxes of ecosystems and to predict the responses of ecosystems to global changes. This study aimed to explore the altitudinal patterns and potential drivers of leaf CratioNratioP stoichiometry. We measured the concentrations of leaf C, N and P in 175 plant species as well as soil nutrient concentrations along an altitudinal transect (500-2300 m) on the northern slope of Changbai Mountain, China to explore the response of leaf CratioNratioP stoichiometry to plant growth form (PGF), climate and soil. Leaf C, N, P and CratioNratioP ratios showed significant altitudinal trends. In general, leaf C and CratioNratioP ratios increased while leaf N and P decreased with elevation. Woody and herbaceous species showed different responses to altitudinal gradients. Trees had the largest variation in leaf C, CratioN and CratioP ratios, while herbs showed the largest variation in leaf N, P and NratioP ratio. PGF, climate and soil jointly regulated leaf stoichiometry, explaining 17.6% to 52.1% of the variation in the six leaf stoichiometric traits. PGF was more important in explaining leaf stoichiometry variation than soil and climate. Our findings will help to elucidate the altitudinal patterns of leaf stoichiometry and to model ecosystem nutrient cycling.
[11]
DRENOVSKY R E, PIETRASIAK N, SHORT T H. Global temporal patterns in plant nutrient resorption plasticity[J]. Global Ecol Biogeogr, 2019,28(6):728-743. DOI: 10.1111/geb.12885.
[12]
江大龙, 徐侠, 阮宏华. 植物养分重吸收及其影响研究进展[J]. 南京林业大学学报(自然科学版), 2017,41(1):183-188.
JIANG D L, XU X, RUAN H H. Review of nutrient resorption and its regulating in plants[J]. J Nanjing For Univ (Nat Sci Ed), 2017,41(1):183-188. DOI: 10.3969/j.issn.1000-2006.2017.01.028.
[13]
张新洁, 陆天宇, 孙海龙, 等. 氮磷添加对水曲柳化学计量特征和养分再吸收的影响[J]. 森林工程, 2019,35(5):16-21.
ZHANG X J, LU T Y, SUN H L, et al. Effects of nitrogen and phosphorus addition on nutrient stoichiometry and resorption of Fraxinus mandshurica[J]. Forest Engineering, 2019,35(5):16-21.
[14]
曾冬萍, 蒋利玲, 曾从盛, 等. 生态化学计量学特征及其应用研究进展[J]. 生态学报, 2013,33(18):5484-5492.
ZENG D P, JIANG L L, ZENG C S, et al. Reviews on the ecological stoichiometry characteristics and its applications[J]. Acta Ecol Sin, 2013,33(18):5484-5492. DOI: 10.5846/stxb201304070628.
[15]
郭子武, 陈双林, 杨清平, 等. 密度对四季竹叶片C、N、P化学计量和养分重吸收特征的影响[J]. 应用生态学报, 2013,24(4):893-899.
GUO Z W, CHEN S L, YANG Q P, et al. Effects of stand density on Oligostachyum lubricum leaf carbon,nitrogen,and phosphorus stoichiometry and nutrient resorption[J]. Chin J Appl Ecol, 2013,24(4):893-899. DOI: 10.13287/j.1001-9332.2013.0243.
[16]
刘广路, 范少辉, 郭宝华, 等. 不同年龄毛竹碳氮磷化学计量特征[J]. 热带作物学报, 2016,37(2):279-285.
LIU G L, FAN S H, GUO B H, et al. The carbon,nitrogen and phosphorus contents of Phyllostachys edulis with different ages[J]. Chin J Trop Crop, 2016,37(2):279-285. DOI: 10.3969/j.issn.1000-2561.2016.02.011.
[17]
叶莉莎, 陈双林. 硝态氮和铵态氮供应比例对雷竹碳、氮、磷化学计量的影响[J]. 植物营养与肥料学报, 2016,22(6):1672-1678.
YE L S, CHEN S L. Effects of nitrate and ammonia supply ratio on the C,N and P stoichiometric characteristics of Phyllostachys violascens[J]. J Plant Nutr Fertil, 2016,22(6):1672-1678. DOI: 10.11674/zwyf.15520.
[18]
HAN W X, FANG J Y, GUO D, et al. Leaf nitrogen and phosphorus stoichiometry across 753 terrestrial plant species in China[J]. New Phytol, 2005,168(2):377-385.DOI: 10.1111/j.1469-8137.2005.01530.x.
Leaf nitrogen and phosphorus stoichiometry of Chinese terrestrial plants was studied based on a national data set including 753 species across the country. Geometric means were calculated for functional groups based on life form, phylogeny and photosynthetic pathway, as well as for all 753 species. The relationships between leaf N and P stoichiometric traits and latitude (and temperature) were analysed. The geometric means of leaf N, P, and N : P ratio for the 753 species were 18.6 and 1.21 mg g(-1) and 14.4, respectively. With increasing latitude (decreasing mean annual temperature, MAT), leaf N and P increased, but the N : P ratio did not show significant changes. Although patterns of leaf N, P and N : P ratios across the functional groups were generally consistent with those reported previously, the overall N : P ratio of China's flora was considerably higher than the global averages, probably caused by a greater shortage of soil P in China than elsewhere. The relationships between leaf N, P and N : P ratio and latitude (and MAT) also suggested the existence of broad biogeographical patterns of these leaf traits in Chinese flora.
[19]
STERNER R W, ELSER J J. Ecological stoichiometry:the biology of elements from molecules to the biosphere[J]. J Plankton Res, 2002,25(9):1183-1183. DOI: 10.1093/plankt/25.9.1183.
[20]
刘敏国, 王士嘉, 陆姣云, 等. 河西走廊藜麦C、N、P生态化学计量学特征对物候期的响应[J]. 干旱区研究, 2018,35(1):192-198.
LIU M G, WANG S J, LU J Y, et al. Response of C,N and P stoichiometry of Chenopodium quinoa to phenological phase in the Hexi Corridor[J]. Arid Zone Res, 2018,35(1):192-198. DOI: 10.13866/j.azr.2018.01.24.
[21]
付作琴, 吕茂奎, 李晓杰, 等. 武夷山不同海拔黄山松新叶和老叶氮磷化学计量特征[J]. 生态学杂志, 2019,38(3):648-654.
FU Z Q, LYU M K, LI X J, et al. Nitrogen and phosphorus stoichiometric characteristics of new and old leaves in Pinus taiwanensis at different altitudes in Wuyi Mountain[J]. Chin J Ecol, 2019,38(3):648-654. DOI: 10.13292/j.1000-4890.201903.020.
[22]
KOERSELMAN W, MEULEMAN A F M. The vegetation N∶P ratio:a new tool to detect the nature of nutrient limitation[J]. J Appl Ecol, 1996,33(6):1441. DOI: 10.2307/2404783.
[23]
WANG M, LIU G H, JIN T T, et al. Age-related changes of leaf traits and stoichiometry in an alpine shrub (Rhododendron agglutinatum) along altitudinal gradient[J]. J Mt Sci, 2017,14(1):106-118. DOI: 10.1007/s11629-016-4096-y.
[24]
LU J Y, YANG M, LIU M G, et al. Leaf stoichiometry and resorption of N and P in Lucerne at different growth stages under different water supplies[J]. J Plant Nutr, 2019,42(1):1-11. DOI: 10.1080/01904167.2019.1567776.
[25]
VERGUTZ L, MANZONI S, PORPORATO A, et al. Global resorption efficiencies and concentrations of carbon and nutrients in leaves of terrestrial plants[J]. Ecol Monogr, 2012,82(2):205-220. DOI: 10.1890/11-0416.1.
Nutrient resorption in plants influences nutrient availability and cycling and is a key process in biogeochemical models. Improved estimates of resorption parameters are needed for predicting long-term primary productivity and for improving such models. Currently, most models assume a value of 50% resorption for nitrogen (N) and phosphorus (P) and lack resorption data for other nutrients and for specific vegetation types. We provide global estimates of resorption efficiencies and nutrient concentrations for carbon (C), N, and P and the first global-scale estimates for essential nutrients such as potassium (K), calcium (Ca), and magnesium (Mg). We also examine leaf mass loss during senescence (LML) globally and for different plant types, thus defining a mass loss correction factor (MLCF) needed to quantify unbiased resorption values. We used a global meta-analysis of 86 studies and; similar to 1000 data points across climates for green and senesced leaves in six plant types: ferns, forbs, graminoids, conifers, and evergreen and deciduous woody angiosperms. In general, N and P resorption differed significantly from the commonly used global value of 50% (62.1%, 64.9%, respectively; P, 0.05). Ca, C, and Mg showed lower average resorptions of 10.9%, 23.2%, and 28.6%, respectively, while K had the highest resorption, at 70.1%. We also found that resorption of all nutrients except Ca depended on leaf nutrient-status; globally, C, N, P, K, and Mg showed a decrease in resorption with increased nutrient status. On average, global leaf mass loss was 24.2%. Overall, our resorption data differ substantially from commonly assumed values and should help improve ecological theory and biogeochemical and land-surface models.
[26]
刘宏伟. 两种不同生态系统中植物叶片功能性状及养分再吸收比较研究[D]. 重庆:西南大学, 2014.
LIU H W. The comparative study on leaf functional traits and nutrient resorption in two different ecosystems of Chongqing[D]. Chongqing:Southwest University, 2014.
[27]
陆姣云, 段兵红, 杨梅, 等. 植物叶片氮磷养分重吸收规律及其调控机制研究进展[J]. 草业学报, 2018,27(4):178-188.
LU J Y, DUAN B H, YANG M, et al. Research progress in nitrogen and phosphorus resorption from senesced leaves and the influence of ontogenetic and environmental factors[J]. Acta Prataculturae Sin, 2018,27(4):178-188. DOI: 10.11686/cyxb2017223.
[28]
SCHREEG L A, SANTIAGO L S, WRIGHT S J, et al. Stem,root,and older leaf N∶P ratios are more responsive indicators of soil nutrient availability than new foliage[J]. Ecology, 2014,95(8):2062-2068. DOI: 10.1890/13-1671.1.
Foliar nitrogen to phosphorus (N:P) ratios are widely used to indicate soil nutrient availability and limitation, but the foliar ratios of woody plants have proven more complicated to interpret than ratios from whole biomass of herbaceous species. This may be related to tissues in woody species acting as nutrient reservoirs during active growth, allowing maintenance of optimal N:P ratios in recently produced, fully expanded leaves (i.e., "new" leaves, the most commonly sampled tissue). Here we address the hypothesis that N:P ratios of newly expanded leaves are less sensitive indicators of soil nutrient availability than are other tissue types in woody plants. Seedlings of five naturally established tree species were harvested from plots receiving two years of fertilizer treatments in a lowland tropical forest in the Republic of Panama. Nutrient concentrations were determined in new leaves, old leaves, stems, and roots. For stems and roots, N:P ratios increased after N addition and decreased after P addition, and trends were consistent across all five species. Older leaves also showed strong responses to N and P addition, and trends were consistent for four of five species. In comparison, overall N:P ratio responses in new leaves were more variable across species. These results indicate that the N:P ratios of stems, roots, and older leaves are more responsive indicators of soil nutrient availability than are those of new leaves. Testing the generality of this result could improve the use of tissue nutrient ratios as indices of soil nutrient availability in woody plants.
[29]
LIN J X, WANG Y N, SUN S N, et al. Effects of arbuscular mycorrhizal fungi on the growth,photosynjournal and photosynthetic pigments of Leymus chinensis seedlings under salt-alkali stress and nitrogen deposition[J]. Sci Total Environ, 2017,576:234-241. DOI: 10.1016/j.scitotenv.2016.10.091.
Leymus chinensis is the most promising grass species for salt-alkaline grassland restoration in northern China. However, little information exists concerning the role of arbuscular mycorrhizal (AM) symbiosis in the adaptation of seedlings to salt-alkali stress, particularly under increased nitrogen deposition, which has become a major environmental problem throughout the world. In this study, Leymus chinensis seedlings were cultivated in soil with 0, 100 and 200mM NaCl/NaHCO3 under two forms of nitrogen (10mM NH4NO3 or NH4Cl: NH4NO3=3:1), and the root colonization, growth and photosynthetic characteristics of the seedlings were measured. The results showed that the colonization rate and intensity decreased with increasing salt-alkali stress and were much lower under alkali stress. The nitrogen treatments also decreased the colonization, particularly under the NH4(+)-N treatment. Compared with the non-mycorrhizal controls, mycorrhizal seedlings generally presented higher plant biomass, photosynthetic parameters and contents of photosynthetic pigments under stresses, and the inhibitive effects of alkali stress were substantially stronger. In addition, both nitrogen forms decreased the physiological indexes compared with those of the AM seedlings. Our results suggest that salt stress and alkali stress are significantly different and that the salt-alkali tolerance of Leymus chinensis seedlings could be enhanced by associations with arbuscular mycorrhizal fungi, in which would yield better plant growth and photosynthesis. Excessive nitrogen in the soil affects mycorrhizal colonization and thereby inhibits the growth and photosynthetic ability of the seedlings.

基金

国际竹藤中心基本科研业务费重点专项(1632018012)

编辑: 李燕文

版权

版权所有,未经授权,不得转载、摘编本刊文章,不得使用本刊的版式设计。
PDF(1557 KB)

Accesses

Citation

Detail

段落导航
相关文章

/