欧洲鹅耳枥水培营养液适用效果比较研究

火艳; 张慧会; 祝遵凌

doi:10.12302/j.issn.1000-2006.201911011

欧洲鹅耳枥水培营养液适用效果比较研究

火艳, 张慧会, 祝遵凌

南京林业大学学报（自然科学版） ›› 2021, Vol. 45 ›› Issue (2) : 17-24.

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南京林业大学学报（自然科学版） ›› 2021, Vol. 45 ›› Issue (2) : 17-24. DOI: 10.12302/j.issn.1000-2006.201911011

专题报道(执行主编方升佐)

欧洲鹅耳枥水培营养液适用效果比较研究

火艳 ¹ ,
张慧会 ¹ ,
祝遵凌 ¹^,²^,^*

作者信息 +

Comparative study on the effects of nutrient solution treatment in hydroponic cultivation of Carpinus betulus

HUO Yan ¹ ,
ZHANG Huihui ¹ ,
ZHU Zunling ¹^,²^,^*

Author information +

文章历史 +

摘要

【目的】探究常用水培营养液的种类、浓度、pH,以及专用营养液对欧洲鹅耳枥水培苗生长的影响,筛选适合欧洲鹅耳枥水培苗生长的最佳营养液,为欧洲鹅耳枥的水培繁殖、生产应用及进一步生理生化研究提供理论依据。【方法】以欧洲鹅耳枥水培苗为试材,以存活率、叶片数、平均根数、平均二级根数和根鲜质量为形态指标,以可溶性糖、淀粉含量、可溶性蛋白含量、POD活性和根系活力为生理指标,采用3因素3水平正交试验研究了常用营养液种类及其浓度、pH对水培苗生长的影响。在筛选出最佳常用营养液的基础上,采用单因素试验研究了最佳常用营养液与自制专用营养液对欧洲鹅耳枥水培苗生长的影响。【结果】营养液正交试验表明,理论上适合欧洲鹅耳枥水培苗生长的常用营养液为1/4浓度的Hewitt通用配方(A3B3),在pH 6.5的该营养液中欧洲鹅耳枥水培苗存活率为85.71%,叶片数为1.60,平均根数为4.40,平均二级根数为25.90,根鲜质量为0.40 g,水培苗的可溶性糖含量、淀粉含量、可溶性蛋白含量和根系活力显著较其他处理高,POD活性显著较其他处理低。最佳常用营养液与专用营养液的对比试验表明,最适合欧洲鹅耳枥水培苗生长的营养液为作者自行配制的专用配方1,在该营养液中欧洲鹅耳枥水培苗存活率为93.33%,可溶性糖含量0.13 mg/g,淀粉含量2.55 mg/g,可溶性蛋白含量33.33 mg/g,根系活力49.75μg/(g·h)。【结论】最适合欧洲鹅耳枥水培苗生长的营养液为作者自行配制的专用配方1。

Abstract

【Objective】To provide a theoretical basis for hydroponic breeding, production, and further physiological and biochemical studies of Carpinus betulus, the effects of type, concentration and pH of ordinary nutrient solution treatment and special nutrient solution for hydroponic seedling growth of C. betulus were examined, and the best nutrient solution for the growth of C. betulus seedlings was screened.【Method】We used C. betulus hydroponic seedlings as the test material. We analyzed the survival rate, number of leaves, average number of roots, average number of second roots and fresh root weight as morphological indexes; and soluble sugar content, starch content, soluble protein content, POD activity and root activity as physical indexes. Hydroponic seedling growth was examined by the type, concentration and pH value of nutrient solution treatment using three factors and three level orthogonal tests. To obtain the best ordinary nutrient solution, we tested hydroponic seedling growth treated with the best ordinary nutrient solutions and special nutrient solutions made by the authors using a single factor test.【Result】The orthogonal test of nutrient solution showed that the most suitable ordinary nutrient solution was the 1/4 Hewitt formula (A3B3). In this condition of pH 6.5, the survival rate was 85.71%, the number of leaves was 1.60, the average number of roots was 4.40, the average number of second roots was 25.90, and the fresh root weight was 0.40 g. The soluble sugar content, starch content, soluble proteins content, and the rooting activity were significantly higher than those in the other treatments, although the POD activity was significantly lower than that in the other treatment. The single factor test of the nutrient solutions showed that the most suitable nutrient solution for C. betulus growth was the special nutrient solution No. 1. Under this condition, the survival rate was 93.33%, the soluble sugar content was 0.13 mg/g, the starch content was 2.55 mg/g, the soluble proteins content was 33.33 mg/g, and the rooting activity was 49.75 μg/(g·h).【Conclusion】The most suitable nutrient solution for C. betulus was the special nutrient solution No.1 prepared by us.

导出引用

火艳, 张慧会, 祝遵凌. 欧洲鹅耳枥水培营养液适用效果比较研究[J]. 南京林业大学学报（自然科学版）. 2021, 45(2): 17-24 https://doi.org/10.12302/j.issn.1000-2006.201911011

HUO Yan, ZHANG Huihui, ZHU Zunling. Comparative study on the effects of nutrient solution treatment in hydroponic cultivation of Carpinus betulus[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2021, 45(2): 17-24 https://doi.org/10.12302/j.issn.1000-2006.201911011

中图分类号： S718;S687

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