短小芽孢杆菌LYMC-3菌株对拟茎点霉的拮抗作用

doi:10.12302/j.issn.1000-2006.202001008

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南京林业大学学报（自然科学版） ›› 2022, Vol. 46 ›› Issue (3): 151-156.doi: 10.12302/j.issn.1000-2006.202001008

短小芽孢杆菌LYMC-3菌株对拟茎点霉的拮抗作用

潘敏(), 朱明龙, 谈家金^*(), 李亮亮, 郝德君

南京林业大学林学院,南方现代林业协同创新中心,江苏南京 210037

收稿日期:2020-01-02 接受日期:2021-12-06 出版日期:2022-05-30 发布日期:2022-06-10
通讯作者: 谈家金
基金资助:
国家重点研发计划(2018YFC1200400)

The antagonism of Bacillus pumilus LYMC-3 strain on Phomopsis macrospora

PAN Min(), ZHU Minglong, TAN Jiajin^*(), LI Liangliang, HAO Dejun

Co-Innovation Center for Sustainable Forestry in Southern China,College of Forestry, Nanjing Forestry University,Nanjing 210037,China

Received:2020-01-02 Accepted:2021-12-06 Online:2022-05-30 Published:2022-06-10
Contact: TAN Jiajin

摘要/Abstract

摘要：

【目的】短小芽孢杆菌LYMC-3菌株对稻瘟病、黄瓜枯萎病及棉花黄萎病等具有显著的防治效果,测定LYMC-3菌株对8种植物病原真菌的抑菌活性,检测其抑菌谱,为其推广应用提供依据。【方法】采用平板对峙法测定LYMC-3菌株对松球壳孢菌(Sphaeropsis sapinea)、松树脂溃疡病菌(Fusarium circinatum)、金黄壳囊孢菌(Cytospora chrysosperma)、拟茎点霉(Phomopsis macrospora)、七叶树壳梭孢菌(Fusicoccum aesculi)、拟盘多毛孢(Pestalotiopsis theae)、山茶炭疽菌(Guignardia camelliae)、葡萄座腔菌(Botryosphaeria dothiorella)8种植物病原真菌的抑菌活性,筛选出抑菌活性最强的病原真菌,进行菌丝生长和孢子萌发抑制试验。【结果】LYMC-3菌株对8种供试植物病原真菌均有抑制作用,抑菌带长度及抑菌活性大小依次为:拟茎点霉>拟盘多毛孢>葡萄座腔菌>松球壳孢菌>七叶树壳梭孢菌>金黄壳囊孢菌>松树脂溃疡病菌>山茶炭疽菌。LYMC-3菌株发酵滤液对拟茎点霉菌丝生长的抑制率为70.36%,对其分生孢子24 h萌发抑制率为88.16%。【结论】短小芽孢杆菌LYMC-3菌株抑菌谱广,其发酵滤液对拟茎点霉有较强的拮抗作用。

关键词: 短小芽孢杆菌, 抑菌谱, 拟茎点霉, 植物病原真菌

Abstract:

【Objective】The Bacillus pumilus LYMC-3 strain has a considerable control effect on the rice blast, cucumber fusarium wilt, and cotton verticillium wilt. To further research on the biocontrol potential of this strain, the antibacterial spectrum was tested by measuring the antibacterial activity of LYMC-3 strain against eight types of plant pathogenic fungi to provide a reference for its subsequent popularization and application.【Method】The antibacterial activity of LYMC-3 strain against eight plant pathogenic fungi (Sphaeropsis sapinea, Fusarium circinatum, Cytospora chrysosperma, Phomopsis macrospora, Fusicoccum aesculi, Pestalotiopsis theae, Guignardia camelliae and Botryosphaeria dothiorella) was determined using the plate pairing method, and one of them was selected for the mycelial growth and spore germination inhibition tests.【Result】The LYMC-3 strain had a wide antimicrobial spectrum, with inhibitory effects on many plant pathogenic fungi that were tested. The length of the inhibitory zone and the magnitude of the antibacterial activity were in the following order: Phomopsis macrospora > Pestalotiopsis theae > Botryosphaeria dothiorella > Sphaeropsis sapinea > Fusicoccum aesculi > Cytospora chrysosperma > Fusarium circinatum > Guignardia camelliae. The inhibition rate of the fermentation broth of the LYMC-3 strain to the mycelial growth of Phomopsis macrospora was 70.36%, and the spore germination in 24 hours was 88.16%.【Conclusion】The Bacillus pumilus LYMC-3 strain has a wide antimicrobial spectrum. The fermentation filtrate broth of the strain has a high antagonistic effect on Phomopsis macrospora.

Key words: Bacillus pumilus, antimicrobial spectrum, Phomopsis macrospora, plant pathogenic fungi

中图分类号:

S763

潘敏,朱明龙,谈家金,等. 短小芽孢杆菌LYMC-3菌株对拟茎点霉的拮抗作用[J]. 南京林业大学学报（自然科学版）, 2022, 46(3): 151-156.

PAN Min, ZHU Minglong, TAN Jiajin, LI Liangliang, HAO Dejun. The antagonism of Bacillus pumilus LYMC-3 strain on Phomopsis macrospora[J].Journal of Nanjing Forestry University (Natural Science Edition), 2022, 46(3): 151-156.DOI: 10.12302/j.issn.1000-2006.202001008.

图/表 4

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处理时间/h treatment time	孢子萌发率/% spore germination rate		萌发抑制率/% spore germination inhibition rate
处理时间/h treatment time	滤液 strain	CK	滤液 strain	CK
4	0.00	5.49	100.00	—
8	1.20	47.16	99.58	—
12	10.21	78.34	86.97	—
24	11.84	100.00	88.16	—

短小芽孢杆菌LYMC-3菌株对拟茎点霉的拮抗作用

The antagonism of Bacillus pumilus LYMC-3 strain on Phomopsis macrospora

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