桢楠种子脱水过程中的生理响应

doi:10.12302/j.issn.1000-2006.202002021

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南京林业大学学报（自然科学版） ›› 2021, Vol. 45 ›› Issue (3): 130-136.doi: 10.12302/j.issn.1000-2006.202002021

桢楠种子脱水过程中的生理响应

李佳琦¹(), 薛晓明², 高捍东¹^,^*()

1.南京林业大学林学院,国家林业和草原局南方林木种子检验中心,南方现代林业协同创新中心,江苏南京 210037
2.南京森林警察学院刑事科学技术学院,野生动植物物证技术国家林业和草原局重点实验室, 江苏南京 210023

收稿日期:2020-02-12 修回日期:2020-08-30 出版日期:2021-05-30 发布日期:2021-05-31
通讯作者: 高捍东
基金资助:
江苏省林业科技创新与推广项目(LYKJ[2019]46)

Physiological responses of Phoebe zhennan seeds during dehydration

LI Jiaqi¹(), XUE Xiaoming², GAO Handong¹^,^*()

1. College of Forestry, Nanjing Forestry University, Southern Tree Seed Inspection Center, National Forestry and Grassland Administration, Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing 210037, China
2. Nanjing Forest Police College, College of Criminal Science and Technology, Key Laboratory of Wildlife Evidence Technology of National Forestry and Grassland Administration, Nanjing 210023, China

Received:2020-02-12 Revised:2020-08-30 Online:2021-05-30 Published:2021-05-31
Contact: GAO Handong

摘要/Abstract

摘要：

【目的】通过对桢楠种子脱水过程中的生理响应进行研究,探讨恰当的脱水处理方式,为桢楠种子的长期保存提供必要的理论和技术支持。【方法】采用硅胶脱水的方法对桢楠种子进行脱水处理,当失水率在0、3%、6%、9%、12%、15%和18%时进行各项生理指标的测定,研究其脱水耐性。【结果】桢楠种子初始含水率为37.74%,初始生活力为89.41%,半致死含水率为29.09%,表现出较高的顽拗性。桢楠种子在硅胶脱水过程中,生活力逐渐下降;电导率和丙二醛(malondialdehyde,MDA)含量不断上升;超氧化物歧化酶(superoxide dismutase,SOD)活性和过氧化氢酶(catalase,CAT)活性均先上升,之后急剧下降;同时,桢楠种子的SOD活性和CAT活性均分别与电导率和MDA含量之间存在显著负相关关系(相关系数分别为-0.833、-0.743、-0.819和-0.871)。随着脱水的进行,桢楠种子保护酶系统受到抑制,膜脂过氧化作用加强,引起其膜透性加剧,有害过氧化物积累,进一步引起种子生活力降低乃至完全丧失。【结论】桢楠种子表现出较高的顽拗性,在储藏过程中应特别注重其对水分的需求,种子储藏的最佳含水率为34.74%,在生产实际中含水率可控制在34.74%±3%。

关键词: 顽坳种子, 脱水耐性, 抗氧化酶, 膜脂过氧化, 种子贮藏

Abstract:

【Objective】The present study aimed to determine the effects of dehydration on the physiological responses of Phoebe zhennan seeds, to provide a basis for a long-term seed preservation. 【Method】Phoebe zhennan seeds were dehydrated using the silica gel. When the water loss reached 0, 3%, 6%, 9%, 12%, 15% and 18%, physiological indexes of the samples were measured. 【Result】The initial water content of P. zhennan seeds was 37.74%, viability was 89.41%, and the half-lethal water content was 29.09%. The viability of P. zhennan seeds gradually decreased during the gel dehydration, and the electrical conductivity and malondialdehyde (MDA) contents continuously increased. Superoxide dismutase (SOD) and catalase (CAT) activities first increased, then sharply decreased. The activities of SOD and CAT negatively correlated with electrical conductivity and MDA content (correlation coefficients: -0.833 and -0.743; -0.819 and -0.871, respectively). The results showed that dehydration inhibited the viability of the protective enzyme system, and strengthened membrane lipid peroxidation. The decrease or complete loss of seed viability led to the increased membrane permeability and peroxide accumulation. 【Conclusion】Water conditions should be carefully managed during storage because P. zhennan seeds are highly recalcitrant. However, slight dehydration can stimulate activity of the protective enzyme (antioxidant) system, which helps to improve the dehydration tolerance. The optimum water content for P. zhennan seeds’ preservation is 34.74%, and in actual production, it can be controlled within its 3%.

Key words: recalcitrant seed, dehydration tolerance, antioxidant enzyme, membrane lipid peroxidation, seed storage

中图分类号:

S722

李佳琦,薛晓明,高捍东. 桢楠种子脱水过程中的生理响应[J]. 南京林业大学学报（自然科学版）, 2021, 45(3): 130-136.

LI Jiaqi, XUE Xiaoming, GAO Handong. Physiological responses of Phoebe zhennan seeds during dehydration[J].Journal of Nanjing Forestry University (Natural Science Edition), 2021, 45(3): 130-136.DOI: 10.12302/j.issn.1000-2006.202002021.

图/表 4

参考文献 29

[1]	国家林业局国有林场和林木种苗工作总站. 中国木本植物种子[M]. 北京: 中国林业出版社, 2001: 207-210.
	State-owned Forest Farm and Forest Seedling Work Station. Seeds of woody plants in China[M]. Beijing: China Forestry Publishing House, 2001: 207-210.
[2]	李锡民. 中国植物志[M]. 北京: 科学出版社, 1982: 113.
	LI X W. Flora of China[M]. Beijing: Science Press, 1982: 113.
[3]	杨娅娟, 郭永杰, 秦少发, 等. 云南九种樟科植物种子的萌发及脱水耐性[J]. 植物分类与资源学报, 2015,37(6):813-820.
	YANG Y J, GUO Y J, QIN S F, et al. Seed storage behavior and seed germination of nine species of Lauraceae from Yunnan, China[J]. Plant Divers Resour, 2015,37(6):813-820. DOI: 10.7677/ynzwyj201515099.
[4]	贾贤, 黄秋生, 刘光华, 等. 我国楠木资源的研究现状[J]. 中国园艺文摘, 2014,30(10):55-59.
	JIA X, HUANG Q S, LIU G H, et al. The present status of Phoebe zhennan[J]. Chin Hortic Abstr, 2014,30(10):55-59.DOI: 10.3969/j.issn.1672-0873.2014.10.023.
[5]	WANG J, NING L P, GAO Q, et al. The identification and characteristics of Phoebe zhennan buried wood[J]. IAWA J, 2019,40(4):804-819.DOI: 10.1163/22941932-40190249. doi: 10.1163/22941932-40190249
[6]	崔令军, 刘瑜霞, 林健, 等. 丛枝菌根真菌对盐胁迫下桢楠光合生理的影响[J]. 南京林业大学学报(自然科学版), 2021,45(1):101-106.
	CUI L J, LIU Y X, LIN J, et al. Effects of AMF on photosynthetic characteristics of Phoebe zhennan under salt stress[J]. J Nanjing For Univ (Nat Sci Ed), 2021,45(1):101-106. DOI: 10.3969/issn.2006-1000.202005007.
[7]	铁得祥, 胡红玲, 喻秀艳, 等. 桢楠幼树光合特性对镉胁迫的响应[J]. 生态学报, 2020,40(11):3738-3746.
	TIE D X, HU H L, YU X Y, et al. Responses of photosynthetic characteristics and chlorophyll fluorescence parameters of Phoebe zhennan saplings to cadmium stress[J]. Acta Ecol Sin, 2020,40(11):3738-3746. DOI: 10.5846/stxb201901250195.
[8]	陈桂琼. 浅谈楠木育苗与造林技术应用[J]. 农业与技术, 2018,38(17):66-67.
	CHEN G Q. Discussion on seedling raising and afforestation technology application of nanmu[J]. Agric Technol, 2018,38(17):66-67. DOI: 10.11974/nyyjs.20180931027.
[9]	张熠. 珍贵树种桢楠的种植技术探讨[J]. 南方农业, 2019,13(32):13-14.
	ZHANG Y. Discussion on planting technology of Phoebe zhennan, a precious tree species[J]. South China Agric, 2019,13(32):13-14. DOI: 10.19415/j.cnki.1673-890x.2019.32.007.
[10]	曹健, 裴云霞, 陈欣媛, 等. 楠木育苗技术研究进展[J]. 湖北民族大学学报(自然科学版), 2020,38(3):270-276.
	CAO J, PEI Y X, CHEN X Y, et al. Advances in seedling propagation technology of Phoebe and Machilus[J]. J Hubei Minzu Univ (Nat Sci Ed), 2020,38(3):270-276. DOI: 10.13501/j.cnki.42-1908/n.2020.09.006.
[11]	李铁华, 文仕知, 喻勋林, 等. 楠木种子活力变化机制的研究[J]. 中国种业, 2008(8):49-51.
	LI T H, WEN S Z, YU X L, et al. Study on seed vigor changing mechanism of Phoeba bournei[J]. China Seed Ind, 2008(8):49-51.DOI: 10.3969/j.issn.1671-895X.2008.08.023.
[12]	李铁华, 文仕知, 彭险峰, 等. 楠木种子活力下降机制研究[J]. 中南林业科技大学学报, 2009,29(5):110-114, 135.
	LI T H, WEN S Z, PENG X F, et al. Dropping mechanism of the vigor of Phoebe zhennan seeds[J]. J Central South Univ For Technol, 2009,29(5):110-114,135.DOI: 10.3969/j.issn.1673-923X.2009.05.015.
[13]	国家质量技术监督局. 林木种子检验规程: GB 2772—1999[S]. 北京:中国标准出版社, 2000.
[14]	温祺. 栓皮栎种子贮藏机理及育苗技术研究[D]. 北京: 北京林业大学, 2010.
	WEN Q. Studies on seed storage mechanism and cultivation technology of Quercus variabilis[D]. Beijing: Beijing Forestry University, 2010.
[15]	胡晋. 种子检验学[M]. 北京: 科学出版社, 2015.
	HU J. Seed testing science[M]. Beijing: Science Press, 2015.
[16]	宋松泉. 种子生物学研究指南[M]. 北京: 科学出版社, 2005.
	SONG S Q. Guidelines for seed biology research [M]. Beijing: Science Press, 2005.
[17]	邹琦. 植物生理学实验指导[M]. 北京: 中国农业出版社, 2000.
	ZOU Q. Experimental guidance of plant physiology [M]. Beijing: Chinese Agriculture Press, 2000.
[18]	尹永强, 胡建斌, 邓明军. 植物叶片抗氧化系统及其对逆境胁迫的响应研究进展[J]. 中国农学通报, 2007,23(1):105-110.
	YIN Y Q, HU J B, DENG M J. Latest development of antioxidant system and responses to stress in plant leaves[J]. Chin Agric Sci Bull, 2007,23(1):105-110.DOI: 10.3969/j.issn.1000-6850.2007.01.025.
[19]	梁新华. 干旱胁迫对甘草种子萌发及CAT活性的影响[J]. 宁夏农林科技, 2004,45(3):1-3.
	LIANG X H. Dry threaten to Glycyrrhiza uralensis Fisch. seed germination and CAT activities[J]. Ningxia J Agric For Sci Technol, 2004,45(3):1-3. DOI: 10.3969/j.issn.1002-204X.2004.03.001.
[20]	ROBERTS E H. Predicting the storage life of seeds[J]. Seed Sci & Technol, 1973(1):449-514.
[21]	PAMMENTER N W, BERJAK P. A review of recalcitrant seed physiology in relation to desiccation-tolerance mechanisms[J]. Seed Sci Res, 1999,9(1):13-37.DOI: 10.1017/s0960258599000033. doi: 10.1017/S0960258599000033
[22]	ELLIS R H, HONG T D, ROBERTS E H. An intermediate category of seed storage behaviour?[J]. J Exp Bot, 1990,41(9):1167-1174. DOI: 10.1093/jxb/41.9.1167. doi: 10.1093/jxb/41.9.1167
[23]	沈海龙. 苗木培育学[M]. 北京: 中国林业出版社, 2009: 30-40.
	SHEN H L. Nursery stock growing[M]. Beijing: China Forestry Publishing House, 2009: 30-40.
[24]	TWEDDLE J C, DICKIE J B, BASKIN C C, et al. Ecological aspects of seed desiccation sensitivity[J]. J Ecol, 2003,91(2):294-304. DOI: 10.1046/j.1365-2745.2003.00760.x. doi: 10.1046/j.1365-2745.2003.00760.x
[25]	JAYASURIYA K M, WIJETUNGA A S, BASKIN J M, et al. Physiological epicotyl dormancy and recalcitrant storage behaviour in seeds of two tropical Fabaceae (subfamily Caesalpinioideae) species[J]. AoB Plants, 2012: pls044. DOI: 10.1093/aobpla/pls044.
[26]	JOSHI G, PHARTYAL S S, ARUNKUMAR A. Non-deep physiological dormancy,desiccation and low-temperature sensitivity in seeds of Garcinia gummi-gutta (Clusiaceae): a tropical evergreen recalcitrant species[J]. Trop Ecol, 2017,58(2):241-250.
[27]	HONG T D, ELLIS R H. A protocol to determine seed storage behavior[J]. International Plant Genetic Resources Institute, 1996: 1-51.
[28]	CHANDEL K P S, CHAUDHURY R, RADHAMANI J, et al. Desiccation and freezing sensitivity in recalcitrant seeds of tea,cocoa and jackfruit[J]. Ann Bot, 1995,76(5):443-450. DOI: 10.1006/anbo.1995.1118. doi: 10.1006/anbo.1995.1118
[29]	BEDI S, BASRA A S. Chilling injury in germinating seeds: basic mechanisms and agricultural implications[J]. Seed Sci Res, 1993,3(4):219-229. DOI: 10.1017/s0960258500001847. doi: 10.1017/S0960258500001847

指标 index	生活力 viability	电导率 electrical conductivity	MDA含量 MDA content	SOD活性 SOD activity	CAT活性 CAT activity
生活力 viability	1	-0.905^**	-0.957^**	0.680	0.945^**
电导率 electrical conductivity		1	0.965^**	-0.833^*	-0.819^*
MDA含量 MDA content			1	-0.743^*	-0.871^*
SOD活性 SOD activity				1	0.746
CAT活性 CAT activity					1

桢楠种子脱水过程中的生理响应

Physiological responses of Phoebe zhennan seeds during dehydration

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