壳寡糖对干旱胁迫下‘凤丹’幼苗生长及生理特性的影响

石欣隆; 杨月琴; 薛娴; 刘伟; 宋程威; 郭丽丽; 侯小改

doi:10.12302/j.issn.1000-2006.202003012

石欣隆, 杨月琴, 薛娴, 刘伟, 宋程威, 郭丽丽, 侯小改

南京林业大学学报（自然科学版） ›› 2021, Vol. 45 ›› Issue (2) : 120-126.

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南京林业大学学报（自然科学版） ›› 2021, Vol. 45 ›› Issue (2) : 120-126. DOI: 10.12302/j.issn.1000-2006.202003012

研究论文

壳寡糖对干旱胁迫下‘凤丹’幼苗生长及生理特性的影响

石欣隆 ¹^,² ,
杨月琴 ¹ ,
薛娴 ¹^,² ,
刘伟 ¹^,² ,
宋程威 ¹^,² ,
郭丽丽 ¹^,² ,
侯小改 ¹^,²^,^*

作者信息 +

Effects of chitooligosaccharide on the growth physiology of Paeonia ostii ‘Feng Dan’ seedlings under drought stress

SHI Xinlong ¹^,² ,
YANG Yueqin ¹ ,
XUE Xian ¹^,² ,
LIU Wei ¹^,² ,
SONG Chengwei ¹^,² ,
GUO Lili ¹^,² ,
HOU Xiaogai ¹^,²^,^*

Author information +

文章历史 +

摘要

【目的】通过研究壳寡糖(chitooligosaccharide,COS)对干旱胁迫下‘凤丹’植株生长和生理特性的影响,明确其对‘凤丹’抗旱性的调控作用,为‘凤丹’苗期培育过程中的环境选择及抗旱性研究提供理论依据。【方法】以两年生油用牡丹‘凤丹’(Paeonia ostii ‘Feng Dan’)盆栽苗为试材,以5个质量浓度(0、20、40、60、80 mg/L)的COS溶液进行喷施,测定不同干旱胁迫下(即断水后第7、9、11、13、15天)‘凤丹’幼苗的生长生理指标。【结果】干旱胁迫下喷施COS能促进‘凤丹’幼苗生长,提高叶片相对含水量、净光合速率和叶绿素含量,提高抗氧化物歧化酶、过氧化物酶和过氧化氢酶活性,提高可溶性糖、可溶性蛋白和脯氨酸含量,降低丙二醛和过氧化氢含量。【结论】喷施40 mg/L COS能显著缓解干旱胁迫对‘凤丹’幼苗造成的伤害(P<0.05),增强植株对干旱胁迫的抵抗能力。

Abstract

【Objective】This study was carried out to investigate the effects of chitooligosaccharide(COS) on the growth, physiology and drought resistance mechanisms of tree peony under drought stress, which provides a theoretical basis for the study of environmental selection and drought resistance during seedling cultivation.【Method】To investigate alleviation of drought stress in plants via COS, biennial Paeonia ostii ‘Feng Dan’ seedlings under different drought stress conditions (water withheld for 7, 9, 11, 13, 15 d) were treated with COS at five concentrations (0, 20, 40, 60, 80 mg/L), and the treated seedlings were collected for physiological analysis.【Result】Compared with the control, treatments that provided exogenous COS during drought stress promoted the growth of tree peony seedlings, thereby markedly improving their relative water content and contents of chlorophyll, soluble sugar(SS), and soluble protein(SP), coupled with an enhanced net photosynthetic rate. Moreover, the superoxide dismutase(SOD), peroxidase(POD), and catalase(CAT) activities of P. ostii ‘Feng Dan’ were significantly increased, and the contents of malondialdehyde(MAD) and H₂O₂ were significantly reduced.【Conclusion】COS treatment at 40 mg/L could effectively alleviate drought damage and enhance resistance in P. ostii ‘Feng Dan’ seedlings.

导出引用

石欣隆, 杨月琴, 薛娴, 等. 壳寡糖对干旱胁迫下‘凤丹’幼苗生长及生理特性的影响[J]. 南京林业大学学报（自然科学版）. 2021, 45(2): 120-126 https://doi.org/10.12302/j.issn.1000-2006.202003012

SHI Xinlong, YANG Yueqin, XUE Xian, et al. Effects of chitooligosaccharide on the growth physiology of Paeonia ostii ‘Feng Dan’ seedlings under drought stress[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2021, 45(2): 120-126 https://doi.org/10.12302/j.issn.1000-2006.202003012

中图分类号： S512.6

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