壳寡糖对干旱胁迫下‘凤丹’幼苗生长及生理特性的影响

doi:10.12302/j.issn.1000-2006.202003012

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南京林业大学学报（自然科学版） ›› 2021, Vol. 45 ›› Issue (2): 120-126.doi: 10.12302/j.issn.1000-2006.202003012

壳寡糖对干旱胁迫下‘凤丹’幼苗生长及生理特性的影响

石欣隆¹^,²(), 杨月琴¹, 薛娴¹^,², 刘伟¹^,², 宋程威¹^,², 郭丽丽¹^,², 侯小改¹^,²^,^*()

1.河南科技大学农学院/牡丹学院,河南洛阳 471023
2.河南省油用牡丹工程技术研究中心,河南洛阳 471023

收稿日期:2020-03-10 接受日期:2020-07-17 出版日期:2021-03-30 发布日期:2021-04-09
通讯作者: 侯小改
基金资助:
国家自然科学基金项目(U1804233);河南省高校重点科研项目(19A180002)

Effects of chitooligosaccharide on the growth physiology of Paeonia ostii ‘Feng Dan’ seedlings under drought stress

SHI Xinlong¹^,²(), YANG Yueqin¹, XUE Xian¹^,², LIU Wei¹^,², SONG Chengwei¹^,², GUO Lili¹^,², HOU Xiaogai¹^,²^,^*()

1. College of Agriculture / College of Tree Penoy,Henan University of Science and Technology,Luoyang 471023,China
2. Henan Province Oil Tree Peony Engineering Technology Research Center,Luoyang 471023,China

Received:2020-03-10 Accepted:2020-07-17 Online:2021-03-30 Published:2021-04-09
Contact: HOU Xiaogai

摘要/Abstract

摘要：

【目的】通过研究壳寡糖(chitooligosaccharide,COS)对干旱胁迫下‘凤丹’植株生长和生理特性的影响,明确其对‘凤丹’抗旱性的调控作用,为‘凤丹’苗期培育过程中的环境选择及抗旱性研究提供理论依据。【方法】以两年生油用牡丹‘凤丹’(Paeonia ostii ‘Feng Dan’)盆栽苗为试材,以5个质量浓度(0、20、40、60、80 mg/L)的COS溶液进行喷施,测定不同干旱胁迫下(即断水后第7、9、11、13、15天)‘凤丹’幼苗的生长生理指标。【结果】干旱胁迫下喷施COS能促进‘凤丹’幼苗生长,提高叶片相对含水量、净光合速率和叶绿素含量,提高抗氧化物歧化酶、过氧化物酶和过氧化氢酶活性,提高可溶性糖、可溶性蛋白和脯氨酸含量,降低丙二醛和过氧化氢含量。【结论】喷施40 mg/L COS能显著缓解干旱胁迫对‘凤丹’幼苗造成的伤害(P<0.05),增强植株对干旱胁迫的抵抗能力。

关键词: ‘凤丹’, 干旱胁迫, 壳寡糖, 生物量, 抗氧化酶, 渗透调节物质

Abstract:

【Objective】This study was carried out to investigate the effects of chitooligosaccharide(COS) on the growth, physiology and drought resistance mechanisms of tree peony under drought stress, which provides a theoretical basis for the study of environmental selection and drought resistance during seedling cultivation.【Method】To investigate alleviation of drought stress in plants via COS, biennial Paeonia ostii ‘Feng Dan’ seedlings under different drought stress conditions (water withheld for 7, 9, 11, 13, 15 d) were treated with COS at five concentrations (0, 20, 40, 60, 80 mg/L), and the treated seedlings were collected for physiological analysis.【Result】Compared with the control, treatments that provided exogenous COS during drought stress promoted the growth of tree peony seedlings, thereby markedly improving their relative water content and contents of chlorophyll, soluble sugar(SS), and soluble protein(SP), coupled with an enhanced net photosynthetic rate. Moreover, the superoxide dismutase(SOD), peroxidase(POD), and catalase(CAT) activities of P. ostii ‘Feng Dan’ were significantly increased, and the contents of malondialdehyde(MAD) and H₂O₂ were significantly reduced.【Conclusion】COS treatment at 40 mg/L could effectively alleviate drought damage and enhance resistance in P. ostii ‘Feng Dan’ seedlings.

Key words: Paeonia ostii ‘Feng Dan’, drought stress, chitooligosaccharide, biomass, antioxidant enzyme, osmoregulatory substance

中图分类号:

S512.6

石欣隆,杨月琴,薛娴,等. 壳寡糖对干旱胁迫下‘凤丹’幼苗生长及生理特性的影响[J]. 南京林业大学学报（自然科学版）, 2021, 45(2): 120-126.

SHI Xinlong, YANG Yueqin, XUE Xian, LIU Wei, SONG Chengwei, GUO Lili, HOU Xiaogai. Effects of chitooligosaccharide on the growth physiology of Paeonia ostii ‘Feng Dan’ seedlings under drought stress[J].Journal of Nanjing Forestry University (Natural Science Edition), 2021, 45(2): 120-126.DOI: 10.12302/j.issn.1000-2006.202003012.

图/表 5

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处理/ (mg·L^-1) treatment	苗高/cm seedling height	茎粗/mm stem diameter	地上生物量/g above-ground biomass	地下生物量/g under-ground biomass	总生物量/g total biomass	根冠比 root-shoot ratio
CK	14.53±1.00 c	4.61±0.27 b	3.72±0.26 b	3.19±0.22 b	6.91±0.48 b	0.86±0.01 a
20	15.33±0.47 bc	5.11±0.23 a	4.29±0.08 b	3.23±0.05 b	7.52±0.03 b	0.75±0.02 b
40	18.23±0.95 a	5.43±0.19 a	5.43±0.26 a	3.85±0.37 a	9.28±0.43 a	0.71±0.08 b
60	16.67±0.81 b	5.18±0.12 a	5.57±0.41 a	3.72±0.28 ab	9.29±0.66 a	0.66±0.03 b
80	16.00±0.76 bc	5.11±0.16 a	5.25±0.54 a	3.45±0.14 ab	8.71±0.49 a	0.66±0.08 b

壳寡糖对干旱胁迫下‘凤丹’幼苗生长及生理特性的影响

Effects of chitooligosaccharide on the growth physiology of Paeonia ostii ‘Feng Dan’ seedlings under drought stress

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