壳寡糖对干旱胁迫下‘凤丹’幼苗生长及生理特性的影响

doi:10.12302/j.issn.1000-2006.202003012

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南京林业大学学报（自然科学版） ›› 2021, Vol. 45 ›› Issue (2): 120-126.doi: 10.12302/j.issn.1000-2006.202003012

壳寡糖对干旱胁迫下‘凤丹’幼苗生长及生理特性的影响

石欣隆¹^,²(), 杨月琴¹, 薛娴¹^,², 刘伟¹^,², 宋程威¹^,², 郭丽丽¹^,², 侯小改¹^,²^,^*()

1.河南科技大学农学院/牡丹学院,河南洛阳 471023
2.河南省油用牡丹工程技术研究中心,河南洛阳 471023

收稿日期:2020-03-10 接受日期:2020-07-17 出版日期:2021-03-30 发布日期:2021-04-09
通讯作者: 侯小改
基金资助:
国家自然科学基金项目(U1804233);河南省高校重点科研项目(19A180002)

Effects of chitooligosaccharide on the growth physiology of Paeonia ostii ‘Feng Dan’ seedlings under drought stress

SHI Xinlong¹^,²(), YANG Yueqin¹, XUE Xian¹^,², LIU Wei¹^,², SONG Chengwei¹^,², GUO Lili¹^,², HOU Xiaogai¹^,²^,^*()

1. College of Agriculture / College of Tree Penoy,Henan University of Science and Technology,Luoyang 471023,China
2. Henan Province Oil Tree Peony Engineering Technology Research Center,Luoyang 471023,China

Received:2020-03-10 Accepted:2020-07-17 Online:2021-03-30 Published:2021-04-09
Contact: HOU Xiaogai

摘要/Abstract

摘要：

【目的】通过研究壳寡糖(chitooligosaccharide,COS)对干旱胁迫下‘凤丹’植株生长和生理特性的影响,明确其对‘凤丹’抗旱性的调控作用,为‘凤丹’苗期培育过程中的环境选择及抗旱性研究提供理论依据。【方法】以两年生油用牡丹‘凤丹’(Paeonia ostii ‘Feng Dan’)盆栽苗为试材,以5个质量浓度(0、20、40、60、80 mg/L)的COS溶液进行喷施,测定不同干旱胁迫下(即断水后第7、9、11、13、15天)‘凤丹’幼苗的生长生理指标。【结果】干旱胁迫下喷施COS能促进‘凤丹’幼苗生长,提高叶片相对含水量、净光合速率和叶绿素含量,提高抗氧化物歧化酶、过氧化物酶和过氧化氢酶活性,提高可溶性糖、可溶性蛋白和脯氨酸含量,降低丙二醛和过氧化氢含量。【结论】喷施40 mg/L COS能显著缓解干旱胁迫对‘凤丹’幼苗造成的伤害(P<0.05),增强植株对干旱胁迫的抵抗能力。

关键词: ‘凤丹’, 干旱胁迫, 壳寡糖, 生物量, 抗氧化酶, 渗透调节物质

Abstract:

【Objective】This study was carried out to investigate the effects of chitooligosaccharide(COS) on the growth, physiology and drought resistance mechanisms of tree peony under drought stress, which provides a theoretical basis for the study of environmental selection and drought resistance during seedling cultivation.【Method】To investigate alleviation of drought stress in plants via COS, biennial Paeonia ostii ‘Feng Dan’ seedlings under different drought stress conditions (water withheld for 7, 9, 11, 13, 15 d) were treated with COS at five concentrations (0, 20, 40, 60, 80 mg/L), and the treated seedlings were collected for physiological analysis.【Result】Compared with the control, treatments that provided exogenous COS during drought stress promoted the growth of tree peony seedlings, thereby markedly improving their relative water content and contents of chlorophyll, soluble sugar(SS), and soluble protein(SP), coupled with an enhanced net photosynthetic rate. Moreover, the superoxide dismutase(SOD), peroxidase(POD), and catalase(CAT) activities of P. ostii ‘Feng Dan’ were significantly increased, and the contents of malondialdehyde(MAD) and H₂O₂ were significantly reduced.【Conclusion】COS treatment at 40 mg/L could effectively alleviate drought damage and enhance resistance in P. ostii ‘Feng Dan’ seedlings.

Key words: Paeonia ostii ‘Feng Dan’, drought stress, chitooligosaccharide, biomass, antioxidant enzyme, osmoregulatory substance

中图分类号:

S512.6

石欣隆,杨月琴,薛娴,等. 壳寡糖对干旱胁迫下‘凤丹’幼苗生长及生理特性的影响[J]. 南京林业大学学报（自然科学版）, 2021, 45(2): 120-126.

SHI Xinlong, YANG Yueqin, XUE Xian, LIU Wei, SONG Chengwei, GUO Lili, HOU Xiaogai. Effects of chitooligosaccharide on the growth physiology of Paeonia ostii ‘Feng Dan’ seedlings under drought stress[J].Journal of Nanjing Forestry University (Natural Science Edition), 2021, 45(2): 120-126.DOI: 10.12302/j.issn.1000-2006.202003012.

图/表 5

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参考文献 32

[1]	GUO L L, GUO D L, ZHAO W, et al. Newly developed SSR markers reveal genetic diversity and geographical clustering in Paeonia suffruticosa based on flower colour[J]. J Hortic Sci Biotechnol, 2018,93(4):416-424. DOI: 10.1080/14620316.2017.1373039.
[2]	赵孟良, 赵文菊, 郭怡婷, 等. 干旱胁迫及复水对菊芋生长及叶片光合和生理特性的影响[J]. 植物资源与环境学报, 2019,28(4):49-57.
	ZHAO M L, ZHAO W J, GUO Y T, et al. Effects of drought stress and rewatering on growth and leaf photosynthetic and physiological characteristics of Helianthus tuberosus[J]. J Plant Resour Environ, 2019,28(4), 49-57 DOI: 10.3969/j.issn.1674-7895.2019.04.06.
[3]	史尚渝, 王飞, 金凯, 等. 基于SPEI的1981—2017年中国北方地区干旱时空分布特征[J]. 干旱地区农业研究, 2019,37(4):215-222.
	SHI S Y, WANG F, JIN K, et al. Temporal and spatial characteristics of drought based on SPEI in northern China from 1981 to 2017[J]. Agric Res Arid Areas, 2019,37(4):215-222. DOI: 10.7606/j.issn.1000-7601.2019.04.29.
[4]	刘曙光, 段佩玲, 张利霞, 等. 氮素形态对‘凤丹’表型性状、光合及产量的影响[J]. 南京林业大学学报(自然科学版), 2019,43(4):161-168.
	LIU S G, DUAN P L, ZHANG L X, et al. Effects of different nitrogen forms on phenotypic traits,photosynjournal and yield of Paeonia ostii‘Feng Dan’[J]. J Nanjing For Univ (Nat Sci Ed), 2019,43(4):161-168. DOI: 10.3969/j.issn.1000-2006.201810010.
[5]	段祥光, 张利霞, 刘伟, 等. 施氮量对油用牡丹‘凤丹’光合特性及产量的影响[J]. 南京林业大学学报(自然科学版), 2018,42(1):48-54.
	DUAN X G, ZHANG L X, LIU W, et al. Effects of nitrogen application on photosynthetic characteristics and yields of oil tree peony Paeonia ostii ‘Feng Dan’[J]. J Nanjing For Univ (Nat Sci Ed), 2018,42(1):48-54. DOI: 10.3969/j.issn.1000-2006.201704033.
[6]	李育材. 中国油用牡丹工程的战略思考[J]. 中国工程科学, 2014, (10):58-63.
	LI Y C. The strategy on the oil tree peony industry in China[J]. Chin Eng Sci, 2014, (10):58-63.DOI: 10.3969/j.issn.1009-1742.2014.10.009.
[7]	LI Y, YIN H, WANG Q, et al. Oligochitosan induced Brassica napus L. production of NO and H₂O₂ and their physiological function[J]. Carbohydr Polym, 2009,75(4):612-617. DOI: 10.1016/j.carbpol.2008.09.005.
[8]	ZHAO X M, SHE X P, DU Y G, et al. Induction of antiviral resistance and stimulary effect by oligochitosan in tobacco[J]. Pestic Biochem Physiol, 2007,87(1):78-84. DOI: 10.1016/j.pestbp.2006.06.006.
[9]	MA L, LI Y, YU C, et al. Alleviation of exogenous oligochitosan on wheat seedlings growth under salt stress[J]. Protoplasma, 2012,249(2):393-399. DOI: 10.1007/s00709-011-0290-5.
[10]	孙君艳, 董丽平, 王付娟. 干旱胁迫下壳寡糖对玉米叶片叶绿素含量、含水量及保护酶活性的影响[J]. 河南农业科学, 2014,43(9):38-40,45.
	SUN J Y, DONG L P, WANG F J. Effects of chitosan oligosaccharide on chlorophyll content,water content and protective enzyme activities of maize under drought stress[J]. J Henan Agric Sci, 2014,43(9):38-40,45. DOI: 10.3969/j.issn.1004-3268.2014.09.009.
[11]	邵晨霞, 胡晋, 宋文坚, 等. 不同酸度壳聚糖溶液引发对玉米发芽和幼苗生理特性的影响[J]. 浙江大学学报(农业与生命科学版), 2005,31(6):705-708.
	SHAO C X, HU J, SONG W J, et al. Effects of seed priming with chitosan solutions of different acidity on seed germination and physiological characteristics of maize seedling[J]. J Zhejiang Univ (Agric Life Sci), 2005,31(6):705-708. DOI: 10.3321/j.issn:1008-9209.2005.06.010.
[12]	靳晓芸. 壳寡糖对甘蔗抗性生理生化的影响[D]. 南宁:广西民族大学, 2010.
	JIN X Y. Effects of chitosan oligosaccharide on the resistant physiologyand biochemistryof sugarcane[D]. Nanning:Guangxi University for Nationalities, 2010.
[13]	邹成林. 壳寡糖对甘蔗干旱胁迫下生理生化及蛋白质差异表达的影响[D]. 南宁:广西民族大学, 2010.
	ZOU C L. Effect of chito-oligosaccharide on the physiological,biochemical and differential protein of sugarcane under drought stress[D]. Nanning:Guangxi University for Nationalities, 2010.
[14]	JIANG H M, YANG J C, ZHANG J F. Effects of external phosphorus on the cell ultrastructure and the chlorophyll content of maize under cadmium and zinc stress[J]. Environ Pollut, 2007,147(3):750-756. DOI: 10.1016/j.envpol.2006.09.006.
[15]	李合生. 现代植物生理学: 植物生理生化实验原理与技术[M].3版. 北京: 高等教育出版社, 2010.
[16]	孔祥生, 易现峰. 植物生理学实验技术[M]. 北京: 中国农业出版社, 2008.
[17]	MUKHTAR AHMED K B, KHAN M M A, SIDDIQUI H, et al. Chitosan and its oligosaccharides,a promising option for sustainable crop production: a review[J]. Carbohydr Polym, 2020,227:115331. DOI: 10.1016/j.carbpol.2019.115331.
[18]	YUAN X B, ZHENG J P, JIAO S M, et al. a review on the preparation of chitosan oligosaccharides and application to human health,animal husbandry and agricultural production[J]. Carbohydr Polym, 2019,220:60-70. DOI: 10.1016/j.carbpol.2019.05.050.
[19]	景红娟, 王石雷, 田航宇, 等. 壳寡糖对小麦早期生长生理的影响[J]. 麦类作物学报, 2013,33(5):1039-1042.
	JING H J, WANG S L, TIAN H Y, et al. Effects of chitosan on early growth and physiology of wheat seedlings[J]. J Triticeae Crop, 2013,33(5):1039-1042.
[20]	LIN Q, ZHANG Y, CUI Y M. Effects of chitooligosaccharides plant growth regulators on the plant growth and secondary metabolites of Astragali[J]. Medicinal Plant, 2010,1(2):11-13.
[21]	李思, 于好强, 付凤玲, 等. 壳寡糖叶面喷施对玉米耐旱性的调节[J]. 玉米科学, 2019,27(3):61-67.
	LI S, YU H Q, FU F L, et al. Regulation effects of foliar spraying with chitosan oligosaccharides on the drought tolerance of maize[J]. J Maize Sci, 2019,27(3):61-67.
[22]	赵莉, 潘远智, 朱峤, 等. 6-BA、GA₃和IBA对香水百合叶绿素含量及抗氧化物酶活性的影响[J]. 草业学报, 2012,21(5):248-256.
	ZHAO L, PAN Y Z, ZHU Q, et al. Effects of 6-BA,GA₃ and IBA on photosynthetic pigment content and related enzyme activities of Lilium casa Blanca[J]. Acta Prataculturae Sin, 2012,21(5):248-256.
[23]	郭卫华, 赵小明, 杜昱光. 壳寡糖对烟草幼苗生长和光合作用及与其相关生理指标的影响[J]. 植物生理学通讯, 2008(6):1155-1157.
	GUO W H, ZHAO X M, DU Y G. Effects of oligochitosan on the growth and photosynjournal and physiological index related to photosynjournal of tobacco seedlings[J]. Plant Physiol Commun, 2008(6):1155-1157.
[24]	李艳, 赵小明, 夏秀英, 等. 壳寡糖对干旱胁迫下油菜光合参数的影响[J]. 作物学报, 2008,34(2):326-329.
	LI Y, ZHAO X M, XIA X Y, et al. Effects of oligochitosan on photosynthetic parameter of brassicanapus seedlings under drought stress[J]. Acta Agron Sin, 2008,34(2):326-329.
[25]	HUSSAIN S, ZHANG J H, ZHONG C, et al. Effects of salt stress on rice growth,development characteristics,and the regulating ways:a review[J]. J Integr Agric, 2017,16(11):2357-2374. DOI: 10.1016/S2095-3119(16)61608-8.
[26]	李伟, 钱永强, 韩蕾, 等. 野牛草克隆分株酶促活性氧清除系统对差异光周期的响应[J]. 西北植物学报, 2015,35(7):1428-1436.
	LI W, QIAN Y Q, HAN L, et al. The response of enzymatic active oxygen scavenging system in leaves of Buchloe dactyloides to differences photoperiod[J]. Acta Bot Boreali-Occidentalia Sin, 2015,35(7):1428-1436. DOI: 10.7606/j.issn.1000-4025.2015.07.1428.
[27]	孙君艳, 李淑梅, 仝胜利. 干旱胁迫下壳寡糖对花生幼苗叶片光合特性及保护酶的影响[J]. 江苏农业科学, 2015,43(6):98-99,100. DOI: 10.15889/j.issn.1002-1302.2015.06.030.
[28]	PHANG T H, SHAO G H, LAM H M. Salt tolerance in soybean[J]. J Integr Plant Biol, 2008,50(10):1196-1212. DOI: 10.1111/j.1744-7909.2008.00760.x.
[29]	刘建新, 欧晓彬, 王金成. 外源H₂O₂对干旱胁迫下裸燕麦幼苗叶片生理特性的影响[J]. 干旱地区农业研究, 2019,37(4):146-153.
	LIU J X, OU X B, WANG J C. Effects of exogenous hydrogen peroxide (H₂O₂) on the physiological characteristics in leaves of Avena nuda L.seedlings under drought stress[J]. Agric Res Arid Areas, 2019,37(4):146-153. DOI: 10.7606/j.issn.1000-7601.2019.04.20.
[30]	石欣隆, 杨月琴, 侯小改, 等. 外源壳寡糖对唐古特白刺抗旱性的影响[J]. 江苏农业科学, 2020,48(13):172-177.
	SHI X L, YANG Y Q, HOU X G, et al. Effects of exogenous COS on drought resistance of Nitraria tangutorum[J]. Jiangsu Agricultural Sciences, 2020,48(13):172-177. DOI: 10.15889/j.issn.1002-1302.2020.13.034.
[31]	李军, 孔祥生, 李金航, 等. 逐渐干旱对牡丹生理指标的影响[J]. 北方园艺, 2014(16):50-53.
	LI J, KONG X S, LI J H, et al. Effect of gradual drought stress on physiological indexes of Paeonia suffruticosa Andr[J]. North Hortic, 2014(16):50-53.
[32]	马莲菊, 张阳, 卜宁, 等. 壳寡糖缓解小麦镉毒害的某些生理特性研究[J]. 环境科学与技术, 2010,33(6):31-34.
	MA L J, ZHANG Y, BU N, et al. Physiological characters of oligochitosan on alleviating Cd toxicity of wheat seedling[J]. Environ Sci Technol, 2010,33(6):31-34. DOI: 10.3969/j.issn.1003-6504.2010.06.008.

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处理/ (mg·L^-1) treatment	苗高/cm seedling height	茎粗/mm stem diameter	地上生物量/g above-ground biomass	地下生物量/g under-ground biomass	总生物量/g total biomass	根冠比 root-shoot ratio
CK	14.53±1.00 c	4.61±0.27 b	3.72±0.26 b	3.19±0.22 b	6.91±0.48 b	0.86±0.01 a
20	15.33±0.47 bc	5.11±0.23 a	4.29±0.08 b	3.23±0.05 b	7.52±0.03 b	0.75±0.02 b
40	18.23±0.95 a	5.43±0.19 a	5.43±0.26 a	3.85±0.37 a	9.28±0.43 a	0.71±0.08 b
60	16.67±0.81 b	5.18±0.12 a	5.57±0.41 a	3.72±0.28 ab	9.29±0.66 a	0.66±0.03 b
80	16.00±0.76 bc	5.11±0.16 a	5.25±0.54 a	3.45±0.14 ab	8.71±0.49 a	0.66±0.08 b

壳寡糖对干旱胁迫下‘凤丹’幼苗生长及生理特性的影响

Effects of chitooligosaccharide on the growth physiology of Paeonia ostii ‘Feng Dan’ seedlings under drought stress

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