硼钼铜微肥配施对太子参光合特性的影响

袁婷婷; 路远峰; 谢寅峰; 马迎莉; 吴桐; 倪震

doi:10.12302/j.issn.1000-2006.202003024

硼钼铜微肥配施对太子参光合特性的影响

袁婷婷, 路远峰, 谢寅峰, 马迎莉, 吴桐, 倪震

南京林业大学学报（自然科学版） ›› 2021, Vol. 45 ›› Issue (4) : 130-136.

PDF(1410 KB)

国家林草科技领军期刊
中国精品科技期刊
中国高校百佳科技期刊
江苏省新闻出版政府奖期刊奖
RCCSE林学权威期刊（A+）
CSCD核心期刊
Scopus数据库收录期刊
中文核心期刊
SCD核心期刊

作者加群：102861116

微信公众号：南京林业大学学报

高级检索

PDF(1410 KB)

南京林业大学学报（自然科学版） ›› 2021, Vol. 45 ›› Issue (4) : 130-136. DOI: 10.12302/j.issn.1000-2006.202003024

研究论文

硼钼铜微肥配施对太子参光合特性的影响

袁婷婷 ¹ ,
路远峰 ¹ ,
谢寅峰 ¹^,^* ,
马迎莉 ¹ ,
吴桐 ² ,
倪震 ²

作者信息 +

Effects of combined application of boron-molybdenum-copper microfertilizers on photosynthetic characteristics of Pseudostellaria heterophylla

YUAN Tingting ¹ ,
LU Yuanfeng ¹ ,
XIE Yinfeng ¹^,^* ,
MA Yingli ¹ ,
WU Tong ² ,
NI Zhen ²

Author information +

文章历史 +

摘要

【目的】探究硼钼铜微肥配施对药用植物太子参光合作用的调控作用及其机制,为太子参高效栽培提供理论依据。【方法】采用二次回归D-最优设计进行田间叶面喷施试验,研究硼钼铜微肥配施对光合气体交换参数和叶绿素荧光参数的影响。【结果】①各微肥配施处理下太子参叶片光合色素含量、净光合速率(P_n)、气孔导度(G_s)、蒸腾速率(T_r)较对照均有不同程度的提高,而胞间CO₂浓度(C_i)则显著降低(P<0.05),说明太子参叶片光合能力的提升主要缘于非气孔限制因素的改善。②试验期间,与对照相比,适宜微肥配比处理不同程度地提高了太子参叶片PSⅡ最大光化学效率(F_v/F_m)、PSⅡ有效光化学效率(F_v'/F_m')、电子传递速率(ETR)和光化学淬灭系数(qP),降低非光化学淬灭系数(NPQ),表明微肥处理能够增强太子参光合系统对光能的吸收、转换与利用效率,缓解过剩激发能所导致的热耗散的增强。③极差分析和单因素方差分析表明,微肥3因素中硼是影响叶片光合性能各参数的主要因素,其次为铜。④通过隶属函数综合分析表明,提高太子参光合性能的最优施肥配方为B₃Mo₂Cu₁,即硼肥1.5 g/L+钼肥0.08 g/L+铜肥0.1 g/L。【结论】适宜的硼钼铜配施能有效提升太子参叶片的光合性能,增强其对光能的吸收、转化和利用效率,降低热耗散并缓解光抑制。

Abstract

【Objective】We studied the regulatory effect of the combined application of B, Mo, and Cu microfertilizers on the photosynthetic characteristics of Pseudostellaria heterophylla to provide a theoretical basis for the efficient cultivation of P. heterophylla. 【Method】A field experiment involving foliar spraying was carried out using a quadratic regression D-optimal design to explore the effects of microfertilizers on photosynthetic gas exchange parameters and fluorescence parameters of P. heterophylla. 【Result】① Compared to the control, the photosynthetic pigment content, net photosynthetic rate (P_n), stomatal conductance (G_s) and transpiration rate (T_r) of the fertilization treatment increased in varying degrees, whereas the intercellular carbon dioxide concentration (C_i) decreased significantly (P<0.05). The results showed that the improvement in photosynthetic capacities of P. heterophylla was mainly due to the relief of non-stomatal limiting factors. ② During the experiment, compared to the control, the PSⅡ maximum photochemical efficiency (F_v/F_m), PSⅡ effective photochemical efficiency (F_v'/F_m'), electron transfer rate (ETR), and photochemical quenching coefficient (qP) of the suitable treatment also increased; whereas, the non-photochemical quenching coefficient (NPQ) decreased. The microfertilizer treatment could enhance the efficiency of the absorption, conversion and utilization of light energy in the photosynthetic system and alleviate the enhanced heat dissipation caused by excess excitation energy. ③ The range analysis and single factor analysis of variance showed that boron played the main role in affecting photosynthetic performance among the three microfertilizers, followed by copper. ④ The comprehensive analysis of membership function showed that the best fertilization formula for improving the photosynthetic performance of P. heterophylla was B₃Mo₂Cu₁ (boron fertilizer 1.5 g/L, molybdenum fertilizer 0.08 g/L and copper fertilizer 0.1 g/L). 【Conclusion】The suitable combined application of boron, molybdenum and copper could effectively improve the photosynthetic performance of P. heterophylla, enhance the efficiency of absorption, transformation and utilization of light energy; while reduce heat dissipation and alleviate photoinhibition.

导出引用

袁婷婷, 路远峰, 谢寅峰, 等. 硼钼铜微肥配施对太子参光合特性的影响[J]. 南京林业大学学报（自然科学版）. 2021, 45(4): 130-136 https://doi.org/10.12302/j.issn.1000-2006.202003024

YUAN Tingting, LU Yuanfeng, XIE Yinfeng, et al. Effects of combined application of boron-molybdenum-copper microfertilizers on photosynthetic characteristics of Pseudostellaria heterophylla[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2021, 45(4): 130-136 https://doi.org/10.12302/j.issn.1000-2006.202003024

中图分类号： S718

参考文献

列表( 原文顺序 | 文献年度倒序 | 文中引用次数倒序 ) 可视化分析

[1]

熊靓, 龚伟, 王景燕, 等. 配方施肥对汉源葡萄青椒叶片光合特性的影响[J]. 西北农林科技大学学报(自然科学版), 2019, 47(1):79-89.

XIONG

, GONG

, WANG J

, et al. Effects of formulated fertilization on photosynthetic characteristics of ‘Hanyuan Putao Qingjiao’[J]. J Northwest A & F Univ (Nat Sci Ed), 2019, 47(1):79-89.DOI: 10.13207/j.cnki.jnwafu.2019.01.010.

https://doi.org/10.13207/j.cnki.jnwafu.2019.01.010

本文引用 [1]

[2]	张立新, 彭连伟, 林荣呈, 等. 光合作用研究进展与前景[J]. 中国基础科学, 2016, 18(1):13-20. ZHANG L X, PENG L W, LIN R C, et al. Advances and prospects of photosynjournal research[J]. China Basic Sci, 2016, 18(1):13-20.DOI: 10.3969/j.issn.1009-2412.2016.01.003. https://doi.org/10.3969/j.issn.1009-2412.2016.01.003 本文引用 [1]

[3]

严田蓉, 何艳, 唐源, 等. 缓释尿素与普通尿素配施对直播杂交籼稻叶片生长及产量的影响[J]. 植物营养与肥料学报, 2019, 25(5):729-740.

YAN T

, HE

, TANG

, et al. Effects of slow-release urea combined with conventional urea on leaf growth and yield formation of indica hybrid rice under direct seeding cultivation[J]. Plant Nutr Fertil Sci, 2019, 25(5):729-740.DOI: 10.11674/zwyf.18194.

https://doi.org/10.11674/zwyf.18194

本文引用 [1]

[4]

季璇, 冯长春, 郑学博, 等. 饼肥等氮替代化肥对植烟土壤养分、酶活性和氮素利用的影响[J]. 中国烟草科学, 2019, 40(5):23-29.

, FENG C

, ZHENG X

, et al. Effects of combined application of rapeseed cake and chemical fertilizers on nutrients,enzyme activity of tobacco growing soil and nitrogen utilization[J]. Chin Tob Sci, 2019, 40(5):23-29.DOI: 10.13496/j.issn.1007-5119.2019.05.004.

https://doi.org/10.13496/j.issn.1007-5119.2019.05.004

本文引用 [1]

[5]	LU Q, XIONG Y, XU L J, et al. Effect of different fertilization patterns on carbon and nitrogen components of tobacco topsoil[J]. Agric Sci Technol, 2014, 15(7):1156-1159,1182. 本文引用 [1]

[6]

位小丫, 林煜, 陈婷, 等. 田间条件下植物促生细菌缓解太子参连作障碍的效果评价[J]. 生态学杂志, 2018, 37(2):399-408.

WEI X

, LIN

, CHEN

, et al. Effects of plant growth-promoting rhizobacteria on alleviating consecutive monoculture problem of Pseudostellaria heterophylla under field conditions[J]. Chin J Ecol, 2018, 37(2):399-408.DOI: 10.13292/j.1000-4890.201802.036.

https://doi.org/10.13292/j.1000-4890.201802.036

本文引用 [1]

[7]	傅兴圣, 刘训红, 许虎, 等. 太子参研究现状与研发趋势[J]. 中国新药杂志, 2012, 21(7):757-760. FU X S, LIU X H, XU H, et al. Research status and trends of Pseudostellariae radix[J]. Chin J New Drugs, 2012, 21(7):757-760. 本文引用 [1]

[8]

马迎莉, 谷慧, 张雨峰, 等. 稀土铈对太子参生长和光合特性的影响[J]. 南京林业大学学报(自然科学版), 2017, 41(4):71-79.

MA Y

, GU

, ZHANG Y

, et al. Effects of cerium nitrate on growth and photosynthetic characteristics of Pseudostellaria heterophylla[J]. J Nanjing For Univ (Nat Sci Ed), 2017, 41(4):71-79.DOI: 10.3969/j.issn.1000-2006.201608009.

https://doi.org/10.3969/j.issn.1000-2006.201608009

本文引用 [1]

[9]

吴玉香, 王汉琪, 沈少炎, 等. 不同施肥方案对太子参活性成分的影响[J]. 江苏农业科学, 2017, 45(6):140-143.

WU Y

, WANG H

, SHENG S

, et al. Effects of different fertilization schemes on active components in Radix pseudostellaria[J]. Jiangsu Agric Sci, 2017, 45(6):140-143. DOI: 10.15889/j.issn.1002-1302.2017.06.036

https://doi.org/10.15889/j.issn.1002-1302.2017.06.036

本文引用 [1]

[10]

武子茜, 袁婧, 曹国璠, 等. “3414”配方施肥对太子参叶片生理指标和产量的影响[J]. 江苏农业科学, 2015, 43(5):238-243.

WU Z

, YUAN

, CAO G

, et al. Effects of “3414” formula fertilization on physiological indexes and yield of Radix pseudostellariae[J]. Jiangsu Agric Sci, 2015, 43(5):238-243.DOI: 10.15889/j.issn.1002-1302.2015.05.079.

https://doi.org/10.15889/j.issn.1002-1302.2015.05.079

本文引用 [1]

[11]

郑听, 曹国璠, 李金玲, 等. 多效唑对太子参光合特性与产量的影响[J]. 贵州农业科学, 2017, 45(2):142-145.

ZHENG

, CAO G

, LI J

, et al. Effects of paclobutrazol on photosynthetic characteristics and yield of Radix pseudostellariae[J]. Guizhou Agric Sci, 2017, 45(2):142-145.DOI: 10.3969/j.issn.1001-3601.2017.02.034.

https://doi.org/10.3969/j.issn.1001-3601.2017.02.034

本文引用 [1]

[12]

宋柏权, 吴贞祯, 赵霄宇, 等. 根外施硼对甜菜叶片性能及块根工艺品质的影响[J]. 中国糖料, 2019, 41(4):50-53.

SONG B

, WU Z

, ZHAO X

, et al. Effect of leaf spraying boron on leaf properties and root quality in sugar beet[J]. Sugar Crop China, 2019, 41(4):50-53.DOI: 10.13570/j.cnki.scc.2019.04.010.

https://doi.org/10.13570/j.cnki.scc.2019.04.010

本文引用 [2]

[13]

NIE Z

, LI S

, HU C

, et al. Effects of molybdenum and phosphorus fertilizers on cold resistance in winter wheat[J]. J Plant Nutr, 2015, 38(5):808-820.DOI: 10.1080/01904167.2014.939289.

https://doi.org/10.1080/01904167.2014.939289

http://www.tandfonline.com/doi/abs/10.1080/01904167.2014.939289

本文引用 [1]

[14]	MOLINA-HEREDIA F P, WASTL J, NAVARRO J A, et al. A new function for an old cytochrome?[J]. Nature, 2003, 424(6944):33-34.DOI: 10.1038/424033b. https://doi.org/10.1038/424033b 本文引用 [1]

[15]

WEIGEL

, VAROTTO

, PESARESI

, et al. Plastocyanin is indispensable for photosynthetic electron flow in Arabidopsis thaliana[J]. J Biol Chem, 2003, 278(33):31286-31289.DOI: 10.1074/jbc.m302876200.

https://doi.org/10.1074/jbc.m302876200

https://linkinghub.elsevier.com/retrieve/pii/S0021925820841726

本文引用 [1]

[16]	刘萍, 李明军. 植物生理学实验技术[M]. 北京: 科学出版社, 2007: 16-18. LIU P, LI M J. Plant physiology technique[M]. Beijing: Science Press, 2007: 16-18. 本文引用 [1]

[17]

冷璐, 陆引罡. 磷、硼肥用量对烤烟石油醚提取物含量的影响[J]. 中国土壤与肥料, 2015(3):40-43.

LENG

, LU Y

. The effects of phosphorus and boron fertilizer application amount on the petroleum extract of flue-cured tobacco[J]. Soils Fertil Sci China, 2015(3):40-43.DOI: 10.11838/sfsc.20150307.

https://doi.org/10.11838/sfsc.20150307

本文引用 [1]

[18]

陆奇杰, 巢建国, 谷巍, 等. 铜胁迫对茅苍术光合特性及生理指标的影响[J]. 中药材, 2018(7):1534-1538.

LU Q

, CHAO J

, GU

, et al. Effects of copper stress on photosynthetic characteristics and physiological indexes of Atractylodes lancea[J]. J Chin Med Mater, 2018(7):1534-1538.DOI: 10.13863/j.issn1001-4454.2018.07.004.

https://doi.org/10.13863/j.issn1001-4454.2018.07.004

本文引用 [1]

[19]

YADAV

, KAUR

, KANWAR M

, et al. Castasterone confers copper stress tolerance by regulating antioxidant enzyme responses,antioxidants,and amino acid balance in B.juncea seedlings[J]. Ecotoxicol Environ Saf, 2018, 147:725-734.DOI: 10.1016/j.ecoenv.2017.09.035.

https://doi.org/10.1016/j.ecoenv.2017.09.035

https://linkinghub.elsevier.com/retrieve/pii/S0147651317306085

本文引用 [1]

[20]	CASSON S, GRAY J E. Influence of environmental factors on stomatal development[J]. New Phytol, 2008, 178(1):9-23.DOI: 10.1111/j.1469-8137.2007.02351.x. https://doi.org/10.1111/j.1469-8137.2007.02351.x https://onlinelibrary.wiley.com/toc/14698137/178/1 本文引用 [1]

[21]	FARQUHAR G D, SHARKEY T D. Stomatal conductance and photosynjournal[J]. Annu Rev Plant Physiol, 1982, 33(1):317-345.DOI: 10.1146/annurev.pp.33.060182.001533. https://doi.org/10.1146/annurev.pp.33.060182.001533 http://www.annualreviews.org/doi/10.1146/annurev.pp.33.060182.001533 本文引用 [1]

[22]

吴敏, 邓平, 赵英, 等. 喀斯特干旱环境对青冈栎叶片生长及叶绿素荧光动力学参数的影响[J]. 应用生态学报, 2019, 30(12):4071-4081.

, DENG

, ZHAO

, et al. Effects of drought on leaf growth and chlorophyll fluorescence kinetics parameters in Cyclobalanopsis glauca seedlings of Karst areas[J]. Chin J Appl Ecol, 2019, 30(12):4071-4081.DOI: 10.13287/j.1001-9332.201912.001.

https://doi.org/10.13287/j.1001-9332.201912.001

本文引用 [1]

[23]	马慧丽, 吕德国. ‘寒富’及其亲本系叶片光合及叶绿素荧光特性的研究[J]. 北方园艺, 2014(20):23-27. MA H L, LYU D G. Study on photosynthetic and chlorophyll fluorescence characte-ristics of ‘Hanfu’ apple and its parents’ variety[J]. North Hortic, 2014(20):23-27. 本文引用 [1]

[24]

丁雷, 李英瑞, 李勇, 等. 梯度干旱胁迫对水稻叶片光合和水分状况的影响[J]. 中国水稻科学, 2014, 28(1):65-70.

DING

, LI Y

, LI

, et al. Effects of drought stress on photosynjournal and water status of rice leaves[J]. Chin J Rice Sci, 2014, 28(1):65-70.DOI: 10.3969/j.issn.1001-7216.2014.01.009.

https://doi.org/10.3969/j.issn.1001-7216.2014.01.009

本文引用 [1]

[25]	侯楠, 李艾莲. 2种株型丹参光合特性研究[J]. 中国农学通报, 2015, 31(16):134-139. HOU N, LI A L. Variation of photosynjournal characteristics in two kinds of Salvia miltiorrhiza[J]. Chin Agric Sci Bull, 2015, 31(16):134-139. 本文引用 [1]