栎丽虎天牛成虫触角感器的扫描电镜观察

doi:10.12302/j.issn.1000-2006.202004059

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南京林业大学学报（自然科学版） ›› 2021, Vol. 45 ›› Issue (5): 195-200.doi: 10.12302/j.issn.1000-2006.202004059

栎丽虎天牛成虫触角感器的扫描电镜观察

尚军烨(), 徐炜超, 孟庆繁, 赵红蕊, 刘生冬, 李燕^*()

北华大学林学院,国家林业和草原局长白山特色森林资源保育与高效利用重点实验室,吉林吉林 132013

收稿日期:2020-04-29 接受日期:2021-03-05 出版日期:2021-09-30 发布日期:2021-09-30
通讯作者: 李燕
基金资助:
国家自然科学基金青年基金项目(31600517);吉林省教育厅项目(JJKH20170042KJ);北华大学青年科技创新团队和北华大学研究生创新项目(2018013)

Antennal sensilla of adult Plagionotus pulcher (Coleoptera: Cerambycidae) observed with scanning electron microscope

SHANG Junye(), XU Weichao, MENG Qingfan, ZHAO Hongrui, LIU Shengdong, LI Yan^*()

Forestry College of Beihua University, Key Laboratory of National Forestry and Grassland Administration for Conservation and Efficient Utilization of Characteristic Forest Resources in Changbai Mountain, Jilin 132013, China

Received:2020-04-29 Accepted:2021-03-05 Online:2021-09-30 Published:2021-09-30
Contact: LI Yan

摘要/Abstract

摘要：

【目的】 明确栎丽虎天牛成虫触角感器类型、数量及分布特征,为其行为和电生理研究提供基础数据。【方法】 利用扫描电镜对栎丽虎天牛雌雄成虫触角表面结构进行观察。【结果】 栎丽虎天牛雌虫触角明显短于体长,雄虫触角短于或等于体长。雌雄虫均有4种类型11个亚型感器,分别为锥形感器6个亚型、毛形感器3个亚型、刺形感器和钟形感器各1个亚型,按感器数量由多到少依次为:毛形感器>锥形感器>刺形感器>钟形感器。锥形感器分布于触角鞭节,毛形感器在触角各节都有分布,刺形感器主要分布在柄节、梗节、鞭节第1~2亚节和部分鞭节亚节的端部,钟形感器分布在雌虫鞭节的第7~9亚节和雄虫的第3~4亚节。感器数量和类型从触角基部向端部逐渐增加,雌虫感器数量多于雄虫。【结论】 触角端部是感受化学物质的主要部位,鞭节第6~9亚节是电生理试验的首选部位。雌虫触角数量感器较多,表明其在接收性信息素和寄主挥发物方面具有更重要的作用,各感器的功能需要结合行为和电生理试验进一步验证。

关键词: 栎丽虎天牛, 触角, 感器, 扫描电镜(SEM)

Abstract:

【Objective】 The type, quantity and distribution of antennal sensilla were studied. The results provide data for the study of Plagionotus pulcher behavior and electrophysiology. 【Method】 Antennae surface structures of male and female adults were observed using a scanning electron microscope. 【Result】 The antennal length of females was significantly shorter than body length; that of males was also shorter than or equal to body length. Male and female adults had six types and 11 subtypes of sensilla. There were six subtypes of sensillum basiconca, three of sensillum trichodea, one of sensillum chaetica and one of sensillum campaniformia. According to the number of sensors, the order from most to least was sensillum trichodea, sensillum basiconca, sensillum chaetica and sensillum campaniformia. Sensillum basiconca were distributed in the flagella; sensillum trichodea, in all antenna segments; and sensillum chaetica, mainly in the scape, pedicel, distal F1, F2 and other falgellum segments. Sensillum campaniformia was distributed from F7 to F9 in females and from F3 to F4 in males. The number of sensilla and types gradually increased from basal to distal, and the antennal sensilla of females was greater than that of males. 【Conclusion】 The distal part of P. pulcher antennae was the main component of semiochemical components, and the segments from F6 to F9 were preferred for electrophysiological testing. More antennal sensilla in females were beneficial for receiving pheromones from males and volatiles from host plants. The function of each sensilla must be further verified using a combination of behavioral and electrophysiological tests.

Key words: Plagionotus pulcher, antennae, sensilla, scanning electron microscope (SEM)

中图分类号:

S763.38

尚军烨,徐炜超,孟庆繁,等. 栎丽虎天牛成虫触角感器的扫描电镜观察[J]. 南京林业大学学报（自然科学版）, 2021, 45(5): 195-200.

SHANG Junye, XU Weichao, MENG Qingfan, ZHAO Hongrui, LIU Shengdong, LI Yan. Antennal sensilla of adult Plagionotus pulcher (Coleoptera: Cerambycidae) observed with scanning electron microscope[J].Journal of Nanjing Forestry University (Natural Science Edition), 2021, 45(5): 195-200.DOI: 10.12302/j.issn.1000-2006.202004059.

图/表 6

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性别 gender	柄节 scape	梗节 pedicel	鞭节flagellum									总计 total
性别 gender	柄节 scape	梗节 pedicel	F1	F2	F3	F4	F5	F6	F7	F8	F9	总计 total
雌虫female	1.02±0.25	0.52±0.08	1.26±0.18	1.26±1.68	1.45±0.19	1.28±0.21	1.16±0.26	0.95±0.22	0.91±0.25	0.75±0.21	0.76±0.26	15.07±1.53
雄虫male	0.91±0.23	0.50±0.09	1.35±0.14	1.33±0.18	1.58±0.14	1.40±0.17	1.58±0.15	1.40±1.69	10.00±1.16	0.88±0.17	0.92±0.20	13.40±1.13

感器类型 type of sensilla	长度/μm length	基部直径/μm basal diameter	端部 tip	表面 surface	形态 shape
锥形感器Ⅰ	8.74±1.12	2.92±0.22	钝	平滑	微弯或平直
锥形感器Ⅱ	13.02±1.86	2.81±0.18	尖	平滑	平直
锥形感器Ⅲ	8.54±0.92	3.01±0.28	钝	平滑	弯曲
锥形感器Ⅳ	9.61±1.87	3.69±0.21	尖	凹陷	平直
锥形感器Ⅴ	6.36±1.14	2.36±0.14	尖	凹陷	平直
锥形感器Ⅵ	6.31±1.02	3.01±0.18	钝	平滑+纵纹	平直
毛形感器Ⅰ	68.77±5.71	4.84±0.26	尖	浅纵纹	微扭曲
毛形感器Ⅱ	39.45±4.11	3.68±0.22	尖	浅纵纹	微弯
毛形感器Ⅲ	25.68±1.25	2.78±0.17	尖	平滑	平直
刺形感器	149.46±23.15	7.38±1.11	尖	纵纹	平直
钟形感器	3.49±0.40	2.69±0.11	钝	平滑	平直

部位segment	B1	B2	B3	B4	B5	B6	Tr1	Tr2	Tr3	Ch1	Sc1	总和total
柄节scape								0.41		15.75		16.16
梗节pedicel								0.34		10.00		10.34
F1								4.73		1.30		6.03
F2								10.13		2.30		12.43
F3	0.71	0.60					35.56	10.06	0.63	2.00		49.59
F4	1.76	1.20		0.60			25.41		0.63			29.60
F5	2.21	4.07	1.30				31.23		2.60			41.41
F6	8.58	2.92	3.46	2.61			37.77			1.00		56.34
F7	12.71	3.71	1.71		0.60	1.30	47.87		1.05		1.33	69.69
F8	13.13	4.87	2.32	2.56	1.30		45.67		1.06		3.84	73.44
F9	11.11	2.07	1.72	1.45		1.36	48.82		1.26		3.53	71.32
总和total	50.21	19.47	10.51	7.22	1.90	2.67	272.33	25.67	7.22	32.35	8.70	436.35

部位 segment	B1	B2	B3	B4	B5	B6	Tr1	Tr2	Tr3	Ch1	Sc1	总和 total
柄节scape										10.36		10.36
梗节pedicel								2.52		9.69		12.21
F1								3.74		2.52		6.26
F2								10.27		2.45		12.72
F3	1.25	5.52					10.71	15.08			0.62	17.41
F4	3.24	6.62		0.60			32.33				1.26	45.30
F5	5.68	4.46		1.30			39.30					50.74
F6	3.81	2.92	1.90	0.60			52.68		0.84			62.75
F7	4.67	2.38	2.52	2.30	0.60	1.30	47.36		1.26			61.79
F8	5.91	2.47	3.36	2.53		1.82	57.48		1.35			74.92
F9	6.33	2.23	2.73	1.30		1.30	52.13		1.26			67.28
总和total	30.89	26.60	10.51	8.63	0.60	4.42	291.99	31.61	4.71	25.26	1.88	422.34

栎丽虎天牛成虫触角感器的扫描电镜观察

Antennal sensilla of adult Plagionotus pulcher (Coleoptera: Cerambycidae) observed with scanning electron microscope

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