机械损伤处理杉木无性系萌蘖及内源激素含量差异

doi:10.12302/j.issn.1000-2006.202006054

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南京林业大学学报（自然科学版） ›› 2021, Vol. 45 ›› Issue (2): 153-158.doi: 10.12302/j.issn.1000-2006.202006054

机械损伤处理杉木无性系萌蘖及内源激素含量差异

张吉玲¹(), 李明阳², 李勇³, 刘丽¹, 费裕翀¹, 曹光球¹^,⁴^,^*()

1.福建农林大学林学院,福建福州 350002
2.福建农林大学植物保护学院,福建福州 350002
3.福建省洋口国有林场,福建顺昌 353200
4.国家林业和草原局杉木工程技术研究中心,福建福州 350002

收稿日期:2020-06-29 接受日期:2020-11-09 出版日期:2021-03-30 发布日期:2021-04-09
通讯作者: 曹光球
基金资助:
国家重点研发计划(2016YFD0600301)

Effects of mechanical damage treatment on the tillering ability and endogenous hormone content of Chinese fir clones

ZHANG Jiling¹(), LI Mingyang², LI Yong³, LIU Li¹, FEI Yuchong¹, CAO Guangqiu¹^,⁴^,^*()

1. College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China
2. College of Plant Protection, Fujian Agricultural and Forestry University, Fuzhou 350002, China
3. Fujian Yangkou State-owned Forest Farm, Shunchang 353200, China
4. China Fir Engineering Research Center, National Forestry and Grassland Administration, Fuzhou 350002, China

Received:2020-06-29 Accepted:2020-11-09 Online:2021-03-30 Published:2021-04-09
Contact: CAO Guangqiu

摘要/Abstract

摘要：

【目的】通过分析机械损伤对不同繁殖方式杉木无性系萌蘖能力的影响,并从内源激素代谢角度阐述其萌蘖机理。【方法】以具有不同萌蘖能力的杉木020扦插苗和020组培苗为试验材料,采用盆栽试验,在分析基部损伤和去顶处理对杉木无性系萌蘖能力影响的基础上,应用酶联免疫吸附法(ELISA)进一步测定了杉木无性系萌蘖高峰期枝叶、基部韧皮部、根尖等不同器官吲哚乙酸(IAA)、赤霉素(GA₃)、脱落酸(ABA)及细胞分裂素(CTK)含量差异,并采用Pearson法分析萌蘖能力与内源激素含量间的相关性。【结果】杉木萌蘖数随着培养时间的延长而增加,去顶和基部损伤处理杉木020扦插苗和020组培苗无性系萌蘖高峰期均为7月,去顶处理杉木无性系萌蘖能力显著高于基部损伤处理,且差异达到显著水平(P < 0.05);随着幼苗的生长,杉木无性系枝叶IAA含量增加,GA₃含量下降;去顶处理有利于杉木幼苗枝叶及根尖ABA的积累,基部损伤处理有利于韧皮部及根尖CTK的积累。【结论】机械损伤条件下,杉木根尖和枝叶中的IAA、ABA和GA₃内源激素含量较高。杉木无性系萌蘖数与机械损伤不同处理呈显著正相关;萌蘖数与GA₃含量呈显著正相关,与IAA、ABA含量呈显著负相关。

关键词: 杉木无性系, 机械损伤, 萌蘖, 内源激素

Abstract:

【Objective】The effect of mechanical damage on the sprouting ability of Cunninghamia lanceolata (Chinese fir) clones with different propagation methods was analyzed, and the sprouting mechanism was expounded from the perspective of endogenous hormone metabolism.【Method】Based on the analysis of the effects of base damage and topping treatments on the sprouting ability of Chinese fir clone 020 cuttings and 020 tissue culture seedlings, indoleacetic acid (IAA), gibberellin (GA₃), abscisic acid (ABA) and cytckinin (CTK) in branches and leaves, base phloem, root tip and other organs of Chinese fir clone at the peak of sprouting were further determined by enzyme-linked immunosorbent assay (ELISA). Pearson method was used to analyze the correlation between sprouting ability and endogenous hormone content.【Result】The number of sprouting tillers increased with the extension of culture time. The peak of sprouting tillers of 020 cuttings and 020 tissue culture seedlings in topping and base damage treatments were in July. The sprouting ability of topping treatments was significantly higher than that of base damage treatments, and the difference reached a significant level (P < 0.05); with the growth of seedlings, IAA content in branches and leaves of Chinese fir clones increased, GA₃ content decreased; topping treatment was conducive to ABA accumulation in branches and leaves and root tips of Chinese fir seedlings, and basal damage treatment was conducive to CTK accumulation in phloem and root tips.【Conclusion】Under the condition of mechanical injury, IAA, ABA and GA₃ endogenous hormone contents in root tips, branches and leaves of Chinese fir were higher. There was a significant positive correlation between the number of sprouting tillers and the mechanical injury treatments, and a significant positive correlation between the number of sprouting tillers and the contents of GA₃, and a significant negative correlation between the number of sprouting tillers and the content of IAA and ABA.

Key words: Cunninghamia lanceolata (Chinese fir) clone, mechanical damage, tillering, endogenous hormones

中图分类号:

S718

张吉玲,李明阳,李勇,等. 机械损伤处理杉木无性系萌蘖及内源激素含量差异[J]. 南京林业大学学报（自然科学版）, 2021, 45(2): 153-158.

ZHANG Jiling, LI Mingyang, LI Yong, LIU Li, FEI Yuchong, CAO Guangqiu. Effects of mechanical damage treatment on the tillering ability and endogenous hormone content of Chinese fir clones[J].Journal of Nanjing Forestry University (Natural Science Edition), 2021, 45(2): 153-158.DOI: 10.12302/j.issn.1000-2006.202006054.

图/表 5

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无性系 clone	处理 treatment	新增萌芽数number of new sprouts
无性系 clone	处理 treatment	2018-04	2018-05	2018-06	2018-07	2018-08
扦插苗 cutting seedlings	基部损伤 basal injury			1.20±0.20 ab	2.10±0.41 b	1.02±0.21 bc
扦插苗 cutting seedlings	去顶topping			1.54±0.22 b	3.12±0.61 a	1.32±0.23 bc
组培苗 tissue culture seedling	基部损伤 basal injury			1.41±0.21 ab	2.32±0.42 bc	1.25±0.23 a
组培苗 tissue culture seedling	去顶topping			1.60±0.23 ab	3.21±0.45 ac	1.41±0.21 bc

项目 item	繁殖方式 mode of reproduction	处理 treatment	器官部位 organ location	萌蘖数 number of sprouts	吲哚乙酸 IAA	赤霉素 GA₃	脱落酸 ABA	细胞分裂素 CTK
繁殖方式mode of reproduction	1.000
处理treatment	0.000	1.000
器官部位organ location	0.000	0.000	1.000
萌蘖数number of sprouts	0.000	0.001^*	0.000	1.000
吲哚乙酸IAA	0.154	-0.286^*	-0.292^*	-0.001^*	1.000
赤霉素GA₃	0.093	0.201	0.064	0.096^*	-0.354^**	1.000
脱落酸ABA	0.234^*	0.222	0.067	-0.193^*	-0.084	0.215	1.000
细胞分裂素CTK	0.038	-0.071	-0.136	-0.210	-0.106	0.010	0.106	1.000

机械损伤处理杉木无性系萌蘖及内源激素含量差异

Effects of mechanical damage treatment on the tillering ability and endogenous hormone content of Chinese fir clones

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