水杨酸对NaHCO3胁迫下桂西北喀斯特地区青冈栎种子萌发的影响

doi:10.12302/j.issn.1000-2006.202008030

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南京林业大学学报（自然科学版） ›› 2021, Vol. 45 ›› Issue (4): 114-122.doi: 10.12302/j.issn.1000-2006.202008030

水杨酸对NaHCO₃胁迫下桂西北喀斯特地区青冈栎种子萌发的影响

邓平¹^,²(), 赵英¹, 王霞¹, 陈秋佑¹, 吴敏¹^,^*()

1.玉林师范学院,桂东南珍稀经济物种保护利用重点实验室培育基地,广西玉林 537000
2.西北农林科技大学林学院,陕西杨凌 712100

收稿日期:2020-08-19 接受日期:2021-01-10 出版日期:2021-07-30 发布日期:2021-07-30
通讯作者: 吴敏
基金资助:
国家自然科学基金项目(31800205);国家自然科学基金项目(31560124);广西自然科学基金项目(2020GXNSFAA259033);广西中青年教师科研基础能力提升项目(2020KY14018);玉林师范学院科研项目(G2020ZK11);大学生创新创业训练计划项目(201710606128)

Effects of salicylic acid on germination of Cyclobalanopsis glauca seeds under NaHCO₃ stress in Karst area of northwest Guangxi

DENG Ping¹^,²(), ZHAO Ying¹, WANG Xia¹, CHEN Qiuyou¹, WU Min¹^,^*()

1. Yulin Normal University, Key Laboratory Breeding Base for the Protection and Utilization of Rare Economic Species in Southeastern Guangxi, Yulin 537000, China
2. College of Forestry, Northwest A & F University, Yangling 712100, China

Received:2020-08-19 Accepted:2021-01-10 Online:2021-07-30 Published:2021-07-30
Contact: WU Min

摘要/Abstract

摘要：

【目的】探讨外源水杨酸(SA)对NaHCO₃胁迫下青冈栎(Cyclobalanopsis glauca )种子萌发特征和生理特性的影响。【方法】研究两个SA浓度(0和0.50 mmol/L)和7个NaHCO₃浓度(0、24、48、72、96、120和144 mmol/L)共14个处理组合下,青冈栎种子萌发参数(发芽率、发芽指数和活力指数)、耐盐指数、相对盐害率、丙二醛(MDA含量)、抗氧化酶[超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)和抗坏血酸过氧化物酶(APX)]活性以及渗透调节物质(可溶性糖、可溶性蛋白和游离脯氨酸)含量的变化。【结果】①无SA处理时,在各NaHCO₃胁迫浓度下,青冈栎种子萌发参数和耐盐指数均低于对照组,相对盐害率则显著高于对照组。经SA处理后,种子萌发参数和耐盐指数均高于无SA处理组,相对盐害率则低于无SA处理组;当NaHCO₃浓度为0~72 mmol/L时,萌发参数和耐盐指数高于对照组,且在NaHCO₃为72 mmol/L时,萌发参数和耐盐指数最高,而相对盐害率最低。②无SA处理时,随NaHCO₃浓度的增加,种子POD和CAT活性先增后减,SOD活性显著低于对照组,MDA含量迅速增加。经SA处理后,在各NaHCO₃胁迫浓度下,种子抗氧化酶活性显著高于无SA处理组,MDA含量显著低于无SA组;且在NaHCO₃为72 mmol/L时,SOD和POD活性最高。③无SA处理组,在NaHCO₃各胁迫浓度下,种子中渗透调节物质都高于对照组;经SA处理后,渗透调节物质有不同程度增加,且在NaHCO₃为72 mmol/L时,其渗透调节物质含量最高。【结论】在喀斯特地区进行青冈栎播种育苗时,用0.50 mmol/L外源SA浸种,对72 mmol/L NaHCO₃胁迫的缓解效应最好。

关键词: 青冈栎, 种子萌发, 水杨酸, 碳酸氢钠, 喀斯特地区

Abstract:

【Objective】This study aimed to alleviate NaHCO₃ stress in Cyclobalanopsis glauca by investigating the effects of exogenous application of salicylic acid (SA) on the seed germination and physiological characteristics, under bicarbonate stress. 【Method】Changes in germination rates and indexes, vigor indexes, salt tolerance indexes, relative salt damage rates, malondialdehyde (MDA) content, antioxidant superoxide dismutase (SOD), peroxidase (POD) and cata-lase (CAT) activities as well as osmotic adjustment substances agents (proline, soluble sugar and soluble protein) content were assessed in C. glauca seeds under NaHCO₃ (0, 24, 48, 72, 96, 120 and 144 mmol/L) stress and exogenous application of salicylic acid (SA: 0 and 0.50 mmol/L) treatment. 【Result】①Germination parameters and salt tole-rance were lower, and salt damage rates were significantly higher under NaHCO₃ stress, without SA, in C. glauca seeds compared to those of the control. However, seeds had higher germination parameters, a higher salt tolerance index, and lower relative salt damage rates under NaHCO₃ stress with SA compared to those without SA. The seed germination parameters and a salt tolerance index were always higher under 0-72 mmol/L NaHCO₃ stress with SA treatment compared with the control group. The germination parameters and a salt tolerance index were maximal, and the relative salt damage rate was minimal under 72 mmol/L NaHCO₃ stress with 0.50 mmol/L of SA treatment. ②The POD and CAT activities of seeds without SA initially increased, then decreased with increasing NaHCO₃ concentrations, and SOD activity was significantly lower than that of the control group, whereas the MDA content rapidly increased. Antioxidant enzyme activities and MDA contents were significantly higher and lower, respectively, under NaHCO₃ stress with, than without SA. The activities of SOD and POD were maximal in 72 mmol/L of NaHCO₃ with 0.50 mmol/L of SA. ③The contents of osmotic adjustment agents were high under NaHCO₃ stress without SA, and increased under NaHCO₃ stress with SA compared with the control. The contents of osmotic adjustment agents were maximal under 72 mmol/L of NaHCO₃ with 0.50 mmol/L of SA.【Conclusion】 The effects of 72 mmol/L NaHCO₃ stress on C. glauca seeds sowed and bred in the Karst areas were alleviated after immersion in 0.50 mmol/L exogenous SA.

Key words: Cyclobalanopsis glauca, seed germination, salicylic acid, NaHCO₃, Karst area

中图分类号:

Q945.79
S718

邓平,赵英,王霞,等. 水杨酸对NaHCO₃胁迫下桂西北喀斯特地区青冈栎种子萌发的影响[J]. 南京林业大学学报（自然科学版）, 2021, 45(4): 114-122.

DENG Ping, ZHAO Ying, WANG Xia, CHEN Qiuyou, WU Min. Effects of salicylic acid on germination of Cyclobalanopsis glauca seeds under NaHCO₃ stress in Karst area of northwest Guangxi[J].Journal of Nanjing Forestry University (Natural Science Edition), 2021, 45(4): 114-122.DOI: 10.12302/j.issn.1000-2006.202008030.

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NaHCO₃ 浓度/ (mmol·L^-1) NaHCO₃ concentration	耐盐指数 salt tolerance index		相对盐害率 relative salt damage rate
NaHCO₃ 浓度/ (mmol·L^-1) NaHCO₃ concentration	0 mmol/L SA	0.50 mmol/L SA	0 mmol/L SA	0.50 mmol/L SA
0	100± 0.00 a	113.22± 7.25 b^*	0 g	-8.97± 0.97 f^**
24	40.92± 5.25 d	111.83± 13.54 b^**	17.24± 1.36 f	3.44± 0.53 d^**
48	60.93± 3.98 c	116.74± 9.85 b^**	20.67± 2.81 e	0.69± 0.10 e^**
72	79.55± 4.25 b	129.34± 10.39 a^**	24.13± 3.45 d	-10.35± 1.89 g^**
96	52.26± 5.48 c	91.90± 7.55 c^**	27.58± 4.01 c	17.24± 1.63 c^**
120	39.45± 2.89 d	75.94± 14.20 e^**	31.03± 2.78 b	24.13± 1.89 b^*
144	29.02± 5.12 e	63.87± 8.65 d^**	37.93± 1.89 a	34.48± 3.33 a