元宝枫组织培养及快速繁殖技术研究

马秋月; 李倩中; 李淑顺; 朱璐; 颜坤元; 李淑娴; 张斌; 闻婧

doi:10.12302/j.issn.1000-2006.202009049

元宝枫组织培养及快速繁殖技术研究

马秋月, 李倩中, 李淑顺, 朱璐, 颜坤元, 李淑娴, 张斌, 闻婧

南京林业大学学报（自然科学版） ›› 2021, Vol. 45 ›› Issue (2) : 220-224.

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南京林业大学学报（自然科学版） ›› 2021, Vol. 45 ›› Issue (2) : 220-224. DOI: 10.12302/j.issn.1000-2006.202009049

研究论文

元宝枫组织培养及快速繁殖技术研究

马秋月 ¹ ,
李倩中 ¹ ,
李淑顺 ¹ ,
朱璐 ¹ ,
颜坤元 ¹ ,
李淑娴 ² ,
张斌 ² ,
闻婧 ¹^,^*

作者信息 +

Study on tissue culture and rapid propagation of Acer truncatum Bunge

MA Qiuyue ¹ ,
LI Qianzhong ¹ ,
LI Shushun ¹ ,
ZHU Lu ¹ ,
YAN Kunyuan ¹ ,
LI Shuxian ² ,
ZHANG Bin ² ,
WEN Jing ¹^,^*

Author information +

文章历史 +

摘要

【目的】通过对元宝枫外植体灭菌方法和培养基配方的筛选,建立元宝枫快速繁殖体系,为开展元宝枫优良种苗繁育推广研究奠定基础,并为元宝枫后期优良种苗的规模化生产提供理论与技术支撑。【方法】以元宝枫带芽茎段为外植体,研究不同消毒剂处理方法、不同基本培养基类型(1/2 MS、MS、1/2 NN69、NN69)以及不同浓度外源生长调节剂(IBA)对腋芽诱导、生根等过程的影响。【结果】元宝枫茎段在体积分数75%乙醇中处理30 s和0.1%HgCl₂灭菌180 s时,成活率最高,但外植体不宜在9月前后采集。在NN69培养基中添加0.2 mg/L IBA时,腋芽诱导率最高(97.2%),且用时最短(10~15 d)。在1/2 NN69中添加0.4 mg/L IBA时有利于生根,生根率可达87.8%。【结论】元宝枫带芽茎段适宜的灭菌方法为75%乙醇处理30 s,再以质量分数0.1% HgCl₂处理180 s;腋芽诱导的适宜培养基为NN69+IBA(0.2 mg/L);适宜元宝枫茎段生根的培养基为1/2 NN69+IBA(0.4 mg/L)。以泥炭、珍珠岩、蛭石体积比为7:2:1的混合基质为移栽基质时,试管苗移栽成活率可达95.0%。

Abstract

【Objective】To establish a rapid propagation system, disinfection method and optimal medium were screened, which will lay the foundation for research on the breeding of Acer truncatum seeding. 【Method】The effects of different sterilization treatment mehods, basic media (1/2 MS, MS, 1/2 NN69, NN69), and plant hormones (IBA) on axillary buds and rooting were investigated using stems with axillary buds of A. truncatum. 【Result】When the stem with axillary buds was sterilized with 75% (volume fraction) alcohol for 30 s and 0.1% (mass fraction) HgCl₂ for 180 s, the survival rate was the highest. The NN69 medium supplemented with 0.2 mg/L IBA showed the highest axillary bud induction rate (97.2%) with the shortest time (10-15 d). The 1/2 NN69 medium supplemented with 0.4 mg/L IBA was suitable for plantlet rooting, with a rooting rate of 87.8%. 【Conclusion】The optimal sterilization method for stem segments of A. truncatum with axillary buds was 75% alcohol for 30 s and 0.1% HgCl₂ for 180 s. The optimal medium for axillary bud induction was NN69 with 0.2 mg/L IBA, and the optimal rooting medium was 1/2 NN69 with 0.4 mg/L IBA. The successful cultivation substrate was prepared by mixing peat, humus,and roseite at a volume ratio of 7:2:1, and the survival rate was 95.0% when the plantlets were moved to the nursery. The results of this study can provide a basis for large-scale propagation of A. truncatum.

导出引用

马秋月, 李倩中, 李淑顺, 等. 元宝枫组织培养及快速繁殖技术研究[J]. 南京林业大学学报（自然科学版）. 2021, 45(2): 220-224 https://doi.org/10.12302/j.issn.1000-2006.202009049

MA Qiuyue, LI Qianzhong, LI Shushun, et al. Study on tissue culture and rapid propagation of Acer truncatum Bunge[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2021, 45(2): 220-224 https://doi.org/10.12302/j.issn.1000-2006.202009049

中图分类号： S718

参考文献

列表( 原文顺序 | 文献年度倒序 | 文中引用次数倒序 ) 可视化分析

[1]	任红剑, 丰震, 乔谦, 等. 元宝枫叶片形态特征的地理变异[J]. 西北林学院学报, 2018,33(1):113-119. REN H J, FENG Z, QIAO Q, et al. Geographic variation trend of leaf morphology in Acer truncatum[J]. J Northwest For Univ, 2018,33(1):113-119.DOI: 10.3969/j.issn.1001-7461.2018.01.18. 本文引用 [1]

[2]	MA Q Y, SUN T L, LI S S, et al. The Acer truncatum genome provides insights into nervonic acid biosynjournal[J]. Plant J, 2020,104(3):662-678.DOI: 10.1111/tpj.14954. 本文引用 [1]

[3]

黄相中, 李聪, 古昆, 等. 元宝枫叶提取物的稳定性及抗氧化活性研究[J]. 云南大学学报(自然科学版), 2003,25(5):442-445,448.

HUANG X

, LI

, GU

, et al. Studies on antioxidative activity and stability of the extracts of Acer truncatum leave[J]. J Yunnan Univ (Nat Sci Ed), 2003,25(5):442-445,448.DOI: 10.3321/j.issn:0258-7971.2003.05.017.

本文引用 [1]

[4]	王性炎, 樊金栓, 王姝清. 中国含神经酸植物开发利用研究[J]. 中国油脂, 2006,31(3):69-71. WANG X Y, FAN J S, WANG S Q. Development situation and outlook of nervonic acid plants in China[J]. China Oils Fats, 2006,31(3):69-71.DOI: 10.3321/j.issn:1003-7969.2006.03.025. 本文引用 [1]

[5]	LI Q, CHEN J, YU X, et al. A mini review of nervonic acid:source,production,and biological functions[J]. Food Chem, 2019,301:125286. DOI: 10.1016/j.foodchem.2019.125286. 本文引用 [1]

[6]	MEDEMA S, MOCKING R J T, KOETER M W J, et al. Levels of red blood cell fatty acids in patients with psychosis,their unaffected siblings,and healthy controls[J]. Schizophr Bull, 2016,42(2):358-368.DOI: 10.1093/schbul/sbv133. 本文引用 [1]

[7]	KAGEYAMA Y, KASAHARA T, NAKAMURA T, et al. Plasma nervonic acid is a potential biomarker for major depressive disorder:a pilot study[J]. Int J Neuropsychopharmacol, 2018,21(3):207-215.DOI: 10.1093/ijnp/pyx089. 本文引用 [1]

[8]	于震宇. 元宝枫愈伤组织培养及其黄酮类化合物积累的研究[D]. 杨凌:西北农林科技大学, 2004. YU Z Y. Studies on callus culture and flavonoid formation of Acer Truncatum Bunge[D]. Yangling:Northwest & University, 2004. 本文引用 [1]

[9]	李艳菊, 陶加洪, 王兰珍, 等. 元宝枫组织培养研究[J]. 北京林业大学学报, 2005,27(3):104-107. LI Y J, TAO J H, WANG L Z, et al. Tissue culture of Acer truncatum[J]. J Beijing For Univ, 2005,27(3):104-107.DOI: 10.3321/j.issn:1000-1522.2005.03.022. 本文引用 [3]

[10]	袁云香, 商澎. 小果卫矛组织培养与快速繁殖[J]. 植物生理学报, 2020,56(6):1243-1247. YUAN Y X, SHANG P. Tissue culture and rapid propagation of Euonymus microcarpus[J]. Plant Physiology Journal, 2020,56(6):1243-1247. DOI: 10.13592/j.cnki.ppj.2020.0100. 本文引用 [1]

[11]	刘占彬, 袁庆华. 多花黑麦草种子外植体组织培养灭菌方法研究[J]. 草地学报, 2009,17(4):474-479. LIU Z B, YUAN Q H. Study on the sterilization methods in tissue culture of Italian ryegrass seed explant[J]. Acta Agrectir Sin, 2009,17(4):474-479.DOI: 10.11733/j.issn.1007-0435.2009.04.012. 本文引用 [1]

[12]	张帆, 叶露, 董姬妃, 等. 高盆樱桃的组培快繁研究[J]. 南京林业大学学报(自然科学版), 2018,42(3):111-116. ZHANG F, YE L, DONG J F, et al. Study on tissue culture and rapid propagation of Cerasus cerasoides var.cerasoides[J]. J Nanjing For Univ (Nat Sci Ed), 2018,42(3):111-116.DOI: 10.3969/j.issn.1000-2006.201706047. 本文引用 [2]

[13]	官锦燕, 谭嘉娜, 罗剑飘, 等. 牛樟的组织培养和植株再生[J]. 南京林业大学学报(自然科学版), 2016,40(4):63-68. GUAN J Y, TAN J N, LUO J P, et al. Tissue culture and plant regeneration of Cinnamomum kanehirae Hay[J]. J Nanjing For Univ (Nat Sci Ed), 2016,40(4):63-68.DOI: 10.3969/j.issn.1000-2006.2016.04.010. 本文引用 [2]

[14]	张静, 牛喆, 范卫芳, 等. 狭叶黄芩组织培养再生体系的建立[J]. 植物研究, 2020,40(1):50-57. ZHANG J, NIU Z, FAN W F, et al. Establishing tissue culture and regeneration system of Scutellaria regeliana[J]. Bull Bot Res, 2020,40(1):50-57. 本文引用 [3]

[15]	苏梦云. 美国枫香茎段组织培养与植株再生[J]. 林业科学研究, 2005,18(1):98-101. SU M Y. Tissue culture of stem segments and plantlet regenation of Liquidambar styraciflua[J]. For Res, 2005,18(1):98-101. 本文引用 [1]

[16]	史港影, 南程慧, 伊贤贵, 等. 雪落樱再生体系的建立[J]. 南京林业大学学报(自然科学版), 2014(S1):20-24. SHI G Y, NAN C H, YI X G, et al. Establishment of regeneration system in Cerasus xueluoensis[J]. J Nanjing For Univ (Nat Sci Ed), 2014(S1):20-24. DOI: 10.3969/j.issn.1000-2006.2014.S1.005. 本文引用 [1]

[17]

牟海飞, 刘洁云, 黄永才, 等. 火龙果离体培养茎段灭菌及芽诱导增殖研究[J]. 西南农业学报, 2017,30(6):1439-1444.

MOU H

, LIU J

, HUANG Y

, et al. Study on stem disinfection,bud induction and proliferation of Hylocereus undatus in vitro culture[J]. Southwest China J Agric Sci, 2017,30(6):1439-1444.DOI: 10.16213/j.cnki.scjas.2017.6.034.

本文引用 [1]

[18]	李艳敏, 孟月娥, 赵秀山, 等. 金叶复叶槭组织培养技术研究[J]. 河南农业科学, 2008,37(7):98-99. LI Y M, MENG Y E, ZHAO X S, et al. Studies on techniques of tissue culture and rapid propagation of Acer negundo ‘Aureomarginatum’[J]. J Henan Agric Sci, 2008,37(7):98-99.DOI: 10.3969/j.issn.1004-3268.2008.07.031. 本文引用 [1]

[19]	李岩岩. 茶条槭组织培养与快速繁殖技术研究[J]. 山东林业科技, 2010,40(2):48-50. LI Y Y. Studies on techniques of tissue culture and rapid propagation of Acer ginnala[J]. J Shandong For Sci Technol, 2010,40(2):48-50.DOI: 10.3969/j.issn.1002-2724.2010.02.015. 本文引用 [1]

[20]	顾地周, 丛小力, 姜云天, 等. 色木槭的组织培养与快速繁殖[J]. 植物生理学通讯, 2008,44(2):314. GU D Z, CONG X L, JIANG Y T, et al. Tissue culture and rapid propagation of Acer mono Maxim[J]. Plant Physiol Commun, 2008,44(2):314. 本文引用 [1]

[21]	王盼盼. 黑木相思不同优良无性系组培快繁技术比较研究[D]. 福州: 福建农林大学, 2011. WANG P P. A comparative study on the techniques of tissue culture and rapid propagation in different fine clones of Acacia melanoxylon[D]. Fuzhou:Fujian Agriculture and Forestry University, 2011. 本文引用 [1]

[22]	李玲莉, 郭素娟, 李吉跃. 北美柔枝松组织培养研究[J]. 北京林业大学学报, 2010,32(3):209-212. LI L L, GUO S J, LI J Y. Tissue culture protocol of limber pine of north America[J]. J Beijing For Univ, 2010,32(3):209-212. 本文引用 [1]

[23]

陈茜, 范佳露, 王宝腾, 等. 落叶型冬青雌、雄株茎段的组织培养技术[J]. 南京林业大学学报(自然科学版), 2017,41(6):181-186.

CHEN

, FAN J

, WANG B

, et al. Micropropagation of Ilex verticillata axillary buds from male and female seedlings[J]. J Nanjing For Univ (Nat Sci Ed), 2017,41(6):181-186.DOI: 10.3969/j.issn.1000-2006.201704002.

本文引用 [1]

[24]	宋跃朋, 陈盼飞, 卜琛皞, 等. 高产优质毛白杨良种组培繁育体系构建[J]. 北京林业大学学报, 2019,41(7):121-127. SONG Y P, CHEN P F, PU C G, et al. Construction of tissue culture breeding system of high yield and quality species in Populus tomentosa[J]. J Beijing For Univ, 2019,41(7):121-127. DOI: 10.13332/j.1000-1522.20180428. 本文引用 [1]