不同供氮水平的核桃幼苗生长及叶绿素荧光特性

doi:10.12302/j.issn.1000-2006.202104016

国家林草科技领军期刊
中国精品科技期刊
中国高校百佳科技期刊
江苏省新闻出版政府奖期刊奖
RCCSE林学权威期刊（A+）
CSCD核心期刊
Scopus数据库收录期刊
中文核心期刊
SCD核心期刊

作者加群：102861116

微信公众号：南京林业大学学报

高级检索

南京林业大学学报（自然科学版） ›› 2022, Vol. 46 ›› Issue (2): 119-126.doi: 10.12302/j.issn.1000-2006.202104016

不同供氮水平的核桃幼苗生长及叶绿素荧光特性

黄小辉¹^,²(), 吴焦焦³, 王玉书², 冯大兰², 孙向阳¹^,^*()

1.北京林业大学林学院,北京 100083
2.三峡库区森林生态保护与恢复重庆市市级重点实验室,重庆 400036
3.四川农业大学林学院,四川成都 611130

收稿日期:2021-04-12 接受日期:2021-10-14 出版日期:2022-03-30 发布日期:2022-04-08
通讯作者: 孙向阳
基金资助:
重庆市科研院所绩效激励引导专项(cstc2018jxjl20001);重庆市科技兴林项目(cstd2019-1)

Growth and chlorophyll fluorescence characteristics of walnut (Juglans regia) seedling under different nitrogen supply levels

HUANG Xiaohui¹^,²(), WU Jiaojiao³, WANG Yushu², FENG Dalan², SUN Xiangyang¹^,^*()

1. Forestry College, Beijing Forestry University, Beijing 100083, China
2. Chongqing Municipal Key Laboratory of Forest Ecological Protection and Restoration in The Three Gorges Reservoir Area, Chongqing 400036, China
3. Forestry College, Sichuan Agricultural University, Chengdu 611130, China

Received:2021-04-12 Accepted:2021-10-14 Online:2022-03-30 Published:2022-04-08
Contact: SUN Xiangyang

摘要/Abstract

摘要：

【目的】探讨核桃对缺氮胁迫的适应性及其机制。【方法】以核桃幼苗为研究对象,设置不同程度的缺氮处理:对照(CK)、中度缺氮(MN)和重度缺氮(SN),分析缺氮胁迫对核桃幼苗外部形态特征、生长情况及叶绿素荧光参数的影响。【结果】缺氮会使核桃幼苗失绿黄化,长势下降,地上部分和地下部分生物量、叶绿素a、叶绿素b和类胡萝卜素含量均显著低于对照,且总体随着缺氮程度加深和时间延长显著下降,尤其是处理后期(60~75 d)表现更明显;MN和SN处理下,核桃幼苗的光系统Ⅱ(PSⅡ)最大光化学效率(F_v/F_m)和光条件下PSⅡ天线转化效率(F_v'/F_m' )在前期(0~30 d)和后期(60~75 d)受影响较大,实际光化学效率(Φ_PSII)、电子传递速率(ETR)和光化学猝灭系数(qP)随胁迫程度加深明显下降,反之非光化学猝灭系数(NPQ)随胁迫程度加深而上升,且同样在处理后期(60~75 d)受影响较大;地上部分生物量与地下部分生物量、叶面积、根表面积、叶绿素a含量、F_v/F_m和ETR呈显著正相关,叶绿素a含量与叶面积、叶片厚度、F_v/F_m 和F_v'/F_m' 显著正相关。【结论】缺氮会抑制核桃幼苗对氮素的吸收和同化,阻碍根系生长,影响核桃幼苗对营养物质的吸收利用,导致植株矮小瘦弱、叶片变小、叶色变淡。在缺氮胁迫下,核桃光合色素含量下降也会造成叶绿体吸收光照的能力减弱,降低光合电子传递速率及对光能的利用效率,进而限制光合速率,影响植株生长。

关键词: 缺氮, 核桃, 形态特征, 叶绿素荧光参数

Abstract:

【Objective】The adaptability of walnut (Juglans regia L.) to nitrogen deficiency and respective adaptive mechanisms were studied.【Method】Different nitrogen deficiency treatments, including a control (CK), moderate nitrogen deficiency (MN), and severe nitrogen deficiency (SN), were used to study the effects of nitrogen deficiency stress on morphological characteristics, growth, and chlorophyll fluorescence parameters of walnut seedlings.【Result】Nitrogen deficiency caused walnut seedlings to lose green but a show yellow coloration, and the growth was decreased. Biomass of the above-ground and below-ground parts, chlorophyll a, chlorophyll b, and carotenoid content were significantly lower compared to the control, and the decrease was more pronounced with the increasing nitrogen deficiency and over time, especially in the late treatment (60-75 d). The values of F_v/F_mand F_v'/F_m' of walnut seedlings treated subjected to MN and SN treatments were significantly affected at the early (0-30 d) and late stage (60-75 d). The actual photochemical efficiency (Φ_PSII), electron transfer rate (ETR), and photochemical quenching coefficient (qP) decreased significantly with the increasing stress, whereas the non-photochemical quenching coefficient (NPQ) increased with the increasing stress, and the effect was more pronounced in the late stage (60-75 d). The aboveground biomass was positively correlated with the belowground biomass, leaf area, root surface area, chlorophyll a content, F_v/F_m, and ETR, whereas the chlorophyll a content was positively correlated with the leaf area, leaf thickness, F_v/F_m, and F_v/F_m'. 【Conclusion】Nitrogen deficiency inhibits absorption and assimilation of nitrogen, hinders the root growth, and affects absorption and utilization of nutrients in walnut seedlings; as a result, the seedlings show dwarfism, weak growth, and smaller leaves with the brighter coloration. Under the nitrogen deficiency stress, the decrease in photosynthetic pigment content in walnuts can also reduce the ability of chloroplasts to absorb light and reduce photosynthetic electron transfer rates and the utilization efficiency of light energy, thereby limiting the photosynthetic rates and plant growth.

Key words: nitrogen deficiency, walnut, morphological characteristics, chlorophyll fluorescence parameters

中图分类号:

S687.9
S718

黄小辉,吴焦焦,王玉书,等. 不同供氮水平的核桃幼苗生长及叶绿素荧光特性[J]. 南京林业大学学报（自然科学版）, 2022, 46(2): 119-126.

HUANG Xiaohui, WU Jiaojiao, WANG Yushu, FENG Dalan, SUN Xiangyang. Growth and chlorophyll fluorescence characteristics of walnut (Juglans regia) seedling under different nitrogen supply levels[J].Journal of Nanjing Forestry University (Natural Science Edition), 2022, 46(2): 119-126.DOI: 10.12302/j.issn.1000-2006.202104016.

图/表 7

表1

图1

表2

表3

表4

图2

表5

核桃幼苗不同指标的相关性分析"

指标 index	ABS	LS	LT	RB	RSA	RSR	Chl a	Chl b	Car	F_v/F_m	$F v'$ / $F m'$	Φ_PSⅡ	ETR	qP	NPQ
ABS	1.00
LS	0.84^*	1.00
LT	0.38	0.75	1.00
RB	0.95^**	0.71	0.21	1.00
RSA	0.89^*	0.75	0.20	0.87^*	1.00
RSR	0.42	0.19	-0.27	0.66	0.58	1.00
Chl a	0.81^*	0.84^*	0.83^*	0.75	0.70	-0.24	1.00
Chl b	-0.42	-0.11	0.34	-0.51	-0.63	-0.56	0.11	1.00
Car	0.14	0.22	0.36	0.02	0.10	-0.27	0.44	-0.35	1.00
F_v/F_m	0.80^*	0.61	0.26	0.35	0.71	0.11	0.83^*	-0.37	0.20	1.00
$F v'$ / $F m'$	0.57	0.40	0.35	-0.08	0.31	-0.37	0.88^*	-0.02	0.32	0.81^*	1.00
Φ_PSⅡ	0.14	0.25	0.54	0.38	0.10	-0.61	0.42	0.34	0.35	0.73	0.83^*	1.00
ETR	0.78^*	0.61	0.51	0.54	0.29	-0.76	0.85^*	0.49	0.28	0.30	0.74	0.96^**	1.00
qP	0.44	0.36	0.54	0.59	0.59	-0.70	0.69	0.63	0.26	-0.24	0.25	0.73	0.78^*	1.00
NPQ	-0.45	-0.13	-0.45	-0.66	-0.61	0.90^**	-0.22	-0.66	-0.23	0.16	-0.33	-0.70	-0.84^*	-0.86^*	1.00

表5

参考文献 29

[1]	傅本重, 邹路路, 朱洁倩, 等. 中国核桃生产现状与发展思路[J]. 江苏农业科学, 2018, 46(18):5-8.
	FU B Z, ZOU L L, ZHU J Q, et al. Current status and development ideas of China’s walnut production[J]. Jiangsu Agric Sci, 2018, 46(18):5-8. DOI: 10.15889/j.issn.1002-1302.2018.18.002. doi: 10.15889/j.issn.1002-1302.2018.18.002
[2]	马庆国, 乐佳兴, 宋晓波, 等. 新中国果树科学研究70年:核桃[J]. 果树学报, 2019, 36(10):1360-1368.
	MA Q G, YUE J X, SONG X B, et al. Fruit scientific research in new China in the past 70 years:walnut[J]. J Fruit Sci, 2019, 36(10):1360-1368. DOI: 10.13925/j.cnki.gsxb.Z10. doi: 10.13925/j.cnki.gsxb.Z10
[3]	郭向华. 主要矿质元素含量与早实核桃产量质量的关系[D]. 保定: 河北农业大学, 2006.
	GUO X H. The relationship of main mineral elements contents and yield,quality of early bearing walnut[D]. Baoding: Hebei Agricultural University, 2006.
[4]	樊卫国, 葛慧敏, 吴素芳, 等. 氮素形态及配比对铁核桃苗生长及营养吸收的影响[J]. 林业科学, 2013, 49(5):77-84.
	FAN W G, GE H M, WU S F, et al. Effect of nitrogen forms and the ratios on growth and nutrient absorption of Juglans sigillata seedling[J]. Sci Silvae Sin, 2013, 49(5):77-84. DOI: 10.11707/j.1001-7488.20130511. doi: 10.11707/j.1001-7488.20130511
[5]	王益明, 卢艺, 张慧, 等. 指数施肥对美国山核桃幼苗生长及叶片养分含量的影响[J]. 中国土壤与肥料, 2018(6):136-140.
	WANG Y M, LU Y, ZHANG H, et al. Effects of exponential fertilization on growth and foliar nutrient status of pecan seedlings[J]. Soils Fertil Sci China, 2018(6):136-140. DOI: 10.11838/sfsc.20180619. doi: 10.11838/sfsc.20180619
[6]	宋岩, 张锐, 鱼尚奇, 等. 不同施氮水平对核桃砧木苗形态特征及生理特性的影响[J]. 福建农业学报, 2020, 35(3):309-316.
	SONG Y, ZHANG R, YU S Q, et al. Morphology and physiology of walnut stock seedlings as affected by nitrogen fertilizations[J]. Fujian J Agric Sci, 2020, 35(3):309-316. DOI: 10.19303/j.issn.1008-0384.2020.03.010. doi: 10.19303/j.issn.1008-0384.2020.03.010
[7]	林郑和, 陈荣冰, 陈常颂. 植物对氮胁迫的生理适应机制研究进展[J]. 湖北农业科学, 2011, 50(23):4761-4764.
	LIN Z H, CHEN R B, CHEN C S. Research progress on physiological adaptability of plants to nitrogen deficiency[J]. Hubei Agric Sci, 2011, 50(23):4761-4764. DOI: 10.14088/j.cnki.issn0439-8114.2011.23.003. doi: 10.14088/j.cnki.issn0439-8114.2011.23.003
[8]	李强, 罗延宏, 龙文靖, 等. 低氮胁迫对不同耐低氮性玉米品种苗期生长和生理特性的影响[J]. 草业学报, 2014, 23(4):204-212.
	LI Q, LUO Y H, LONG W J, et al. Effect of low nitrogen stress on different low nitrogen tolerance maize cultivars seedling stage growth and physiological characteristics[J]. Acta Prataculturae Sin, 2014, 23(4):204-212. DOI: 10.11686/cyxb20140425. doi: 10.11686/cyxb20140425
[9]	EVANS J R, CLARKE V C. The nitrogen cost of photosynthesis[J]. J Exp Bot, 2019, 70(1):7-15. DOI: 10.1093/jxb/ery366. doi: 10.1093/jxb/ery366
[10]	LIU X, YIN C M, XIANG L, et al. Transcription strategies related to photosynthesis and nitrogen metabolism of wheat in response to nitrogen deficiency[J]. BMC Plant Biol, 2020, 20(1):448. DOI: 10.1186/s12870-020-02662-3. doi: 10.1186/s12870-020-02662-3
[11]	蔡瑞国, 张敏, 戴忠民, 等. 施氮水平对优质小麦旗叶光合特性和子粒生长发育的影响[J]. 植物营养与肥料学报, 2006, 12(1):49-55.
	CAI R G, ZHANG M, DAI Z M, et al. Effects of nitrogen application rate on flag leaf photosynthetic characteristics and grain growth and development of high-quality wheat[J]. Plant Nutr Fertil Sci, 2006, 12(1):49-55. DOI: 10.3321/j.issn:1008-505X.2006.01.009. doi: 10.3321/j.issn:1008-505X.2006.01.009
[12]	ANTAL T, MATTILA H, HAKALA-YATKIN M, et al. Acclimation of photosynthesis to nitrogen deficiency in Phaseolus vulgaris[J]. Planta, 2010, 232(4):887-898. DOI: 10.1007/s00425-010-1227-5. doi: 10.1007/s00425-010-1227-5
[13]	黄小辉, 冯大兰, 刘芸, 等. 模拟石漠化异质生境中桑树的生长和叶绿素荧光特性[J]. 北京林业大学学报, 2016, 38(10):50-58.
	HUANG X H, FENG D L, LIU Y, et al. Growth and chlorophyll fluorescence characteristics of mulberry trees in simulated environment of heterogeneous habitats of a rocky desertification area[J]. J Beijing For Univ, 2016, 38(10):50-58. DOI: 10.13332/j.1000-1522.20150324. doi: 10.13332/j.1000-1522.20150324
[14]	WEI W, YE C, HUANG H C, et al. Appropriate nitrogen application enhances saponin synthesis and growth mediated by optimizing root nutrient uptake ability[J]. J Ginseng Res, 2020, 44(4):627-636. DOI: 10.1016/j.jgr.2019.04.003. doi: 10.1016/j.jgr.2019.04.003
[15]	陈静, 刘连涛, 孙红春, 等. 外源NO对缺氮胁迫下棉花幼苗形态及生长的调控效应[J]. 中国农业科学, 2014, 47(23):4565-4575.
	CHEN J, LIU L T, SUN H C, et al. Regulatory effects of exogenous nitric oxide on morphology of cotton seedlings under nitrogen stress[J]. Sci Agric Sin, 2014, 47(23):4565-4575. DOI: 10.3864/j.issn.0578-1752.2014.23.005. doi: 10.3864/j.issn.0578-1752.2014.23.005
[16]	刘芳, 林李华, 张立丹, 等. 缺氮和恢复供氮对香蕉苗生长和根系形态参数的影响[J]. 果树学报, 2019, 36(1):67-75.
	LIU F, LIN L H, ZHANG L D, et al. Effects of N deficiency and resupply of N nutrient on banana growth and root morphological parameters[J]. J Fruit Sci, 2019, 36(1):67-75. DOI: 10.13925/j.cnki.gsxb.20180168. doi: 10.13925/j.cnki.gsxb.20180168
[17]	TRUBAT R, CORTINA J, VILAGROSA A. Plant morphology and root hydraulics are altered by nutrient deficiency in Pistacia lentiscus (L.)[J]. Trees, 2006, 20(3):334-339. DOI: 10.1007/s00468-005-0045-z. doi: 10.1007/s00468-005-0045-z
[18]	FERREIRA V S, PINTO R F, SANT’ANNA C. Low light intensity and nitrogen starvation modulate the chlorophyll content of Scenedesmus dimorphus[J]. J Appl Microbiol, 2016, 120(3):661-670. DOI: 10.1111/jam.13007. doi: 10.1111/jam.13007
[19]	朱超, 冀爱青, 宁婵娟, 等. 氮素形态对早实核桃叶片光合特性和果实品质的影响[J]. 河南农业大学学报, 2012, 46(5):526-529,534.
	ZHU C, JI A Q, NING C J, et al. Effects of different forms of nitrogen on leaf photosynthetic characteristics and nuts quality of early-fruiting walnut[J]. J Henan Agric Univ, 2012, 46(5):526-529,534. DOI: 10.16445/j.cnki.1000-2340.2012.05.016. doi: 10.16445/j.cnki.1000-2340.2012.05.016
[20]	ROSCIOLI J D, GHOSH S, LAFOUNTAIN A M, et al. Quantum coherent excitation energy transfer by carotenoids in photosynthetic light harvesting[J]. J Phys Chem Lett, 2017, 8(20):5141-5147. DOI: 10.1021/acs.jpclett.7b01791. doi: 10.1021/acs.jpclett.7b01791
[21]	PAN S G, LIU H D, MO Z W, et al. Effects of nitrogen and shading on root morphologies,nutrient accumulation,and photosynthetic parameters in different rice genotypes[J]. Sci Rep, 2016, 6:32148. DOI: 10.1038/srep32148. doi: 10.1038/srep32148
[22]	ZHANG T, YANG S B, GUO R, et al. Warming and nitrogen addition alter photosynthetic pigments,sugars and nutrients in a temperate meadow ecosystem[J]. PLoS One, 2016, 11(5):e0155375. DOI: 10.1371/journal.pone.0155375. doi: 10.1371/journal.pone.0155375
[23]	李小涵, 武建军, 吕爱锋, 等. 不同CO₂浓度变化下干旱对冬小麦叶面积指数的影响差异[J]. 生态学报, 2013, 33(9):2936-2943.
	LI X H, WU J J, LÜ A F, et al. The difference of drought impacts on winter wheat leaf area index under different CO₂ concentration[J]. Acta Ecol Sin, 2013, 33(9):2936-2943.
[24]	高雪, 贾中立, 林凯丽, 等. 水旱条件下小麦叶面积指数和叶绿素含量QTL定位[J]. 植物遗传资源学报, 2021, 22(4):1109-1119.
	GAO X, JIA Z L, LIN K L, et al. QTL mapping of leaf area index and chlorophyll content in wheat with normal irrigation and under drought stress[J]. J Plant Genet Resour, 2021, 22(4):1109-1119. DOI: 10.13430/j.cnki.jpgr.20210129002. doi: 10.13430/j.cnki.jpgr.20210129002
[25]	YAO X D, LI C H, LI S Y, et al. Effect of shade on leaf photosynthetic capacity,light-intercepting,electron transfer and energy distribution of soybeans[J]. Plant Growth Regul, 2017, 83(3):409-416. DOI: 10.1007/s10725-017-0307-y. doi: 10.1007/s10725-017-0307-y
[26]	张青青, 周再知, 王西洋, 等. 施肥对柚木光合生理和叶绿素荧光特性的影响[J]. 中南林业科技大学学报, 2021, 41(4):31-38.
	ZHANG Q Q, ZHOU Z Z, WANG X Y, et al. Effects of fertilization on photosynthetic and chlorophyll fluorescence characteristics of Tectona grandis[J]. J Central South Univ For Technol, 2021, 41(4):31-38. DOI: 10.14067/j.cnki.1673-923x.2021.04.004. doi: 10.14067/j.cnki.1673-923x.2021.04.004
[27]	PARK S, FISCHER A L, STEEN C J, et al. Chlorophyll-carotenoid excitation energy transfer in high-light-exposed thylakoid membranes investigated by snapshot transient absorption spectroscopy[J]. J Am Chem Soc, 2018, 140(38):11965-11973. DOI: 10.1021/jacs.8b04844. doi: 10.1021/jacs.8b04844
[28]	WANG Y, JIN W W, CHE Y H, et al. Atmospheric nitrogen dioxide improves photosynthesis in mulberry leaves via effective utilization of excess absorbed light energy[J]. Forests, 2019, 10(4):312. DOI: 10.3390/f10040312. doi: 10.3390/f10040312
[29]	相昆, 李宪利, 王晓芳, 等. 水分胁迫下外源NO对核桃叶绿素荧光的影响[J]. 果树学报, 2006, 23(4):616-619.
	XIANG K, LI X L, WANG X F, et al. Effects of exogenous nitric oxide on the chlorophyll fluorescence parameters of walnut under water stress[J]. J Fruit Sci, 2006, 23(4):616-619. DOI: 10.3969/j.issn.1009-9980.2006.04.029. doi: 10.3969/j.issn.1009-9980.2006.04.029

营养条件 nutrient conditions		处理 treatment
营养条件 nutrient conditions		CK	MN	SN
大量元素用量/ (mg·L^-1) macro element	Ca(NO₃)₂·4H₂O	945	472.5	—
	KNO₃	607	303.5	—
	NH₄H₂PO₄	115	57.5	—
	MgSO₄	493.0	493.0	493.0
	CaCl₂	—	222.2	444.5
	KCl	—	223.7	447.3
	NaH₂PO₄·H₂O	—	70.9	141.7
铁盐用量/(g·L^-1) (pH=5.5) iron salt	FeSO₄·7H₂O	5.56	5.56	5.56
铁盐用量/(g·L^-1) (pH=5.5) iron salt	Na₂EDTA	7.46	7.46	7.46
微量元素用量/ (mg·L^-1) (pH=6.0) micro element	KI	0.83	0.83	0.83
	MnSO₄	22.30	22.30	22.30
	Na₂MoO₄	0.25	0.25	0.25
	CuSO₄	0.025	0.025	0.025
	CoCl₂	0.025	0.025	0.025
	H₃BO₃	6.20	6.20	6.20
	ZnSO₄	8.60	8.60	8.60

处理时间/d treatment time	地上部分生物量/g above-ground biomass			叶面积/cm² leaf area			叶片厚度/mm leaf thickness
处理时间/d treatment time	CK	MN	SN	CK	MN	SN	CK	MN	SN
0	1.58±0.09 Fa	1.63±0.08 Fa	1.59±0.09 Ea	59.4±3.08 Ca	57.8±3.10 Ea	58.2±4.09 Ba	0.15±0.01 Ba	0.16±0.01 Ba	0.14±0.01 Aa
15	3.13±0.18 Ea	2.09±0.12 Eb	2.28±0.11 Db	95.5±7.21 Ba	65.3±6.15 Db	59.9±6.13 Bb	0.21±0.01 Aa	0.16±0.02 Bb	0.15±0.01 Ab
30	5.95±0.24 Da	4.14±0.27 Db	3.54±0.19 Cc	96.8±8.20 Ba	77.3±6.16 Cb	61.2±5.18 Bc	0.22±0.02 Aa	0.19±0.01 Ab	0.13±0.01 Ac
45	11.38±0.59 Ca	9.34±0.43 Cb	6.34±0.32 Bc	112.3±8.45 Aa	89.7±6.39 Bb	66.8±6.23 Ac	0.22±0.02 Aa	0.16±0.01 Bb	0.13±0.01 Ac
60	17.43±0.83 Ba	13.24±0.64 Bb	7.28±0.46 Ac	115.4±9.85 Aa	96.6±7.69 Ab	69.0±6.52 Ac	0.19±0.01 Aa	0.17±0.01 Ba	0.12±0.02 Ab
75	24.61±1.69 Aa	14.48±1.12 Ab	7.57±0.63 Ac	119.3±9.83 Aa	97.9±7.18 Ab	70.9±7.13 Ac	0.21±0.01 Aa	0.15±0.01 Bb	0.13±0.01 Aa

处理时间/d treatment time	根系生物量/g root biomass			根系表面积/cm² root surface-area			根冠比/% root-shoot ratio
处理时间/d treatment time	CK	MN	SN	CK	MN	SN	CK	MN	SN
0	1.12±0.08 Fa	1.21±0.10 Fa	1.17±0.09 Fa	35.9±3.4 Fa	38.4±3.7 Fa	36.8±3.2 Fa	0.71±0.05 Ba	0.74±0.06 Da	0.73±0.05 Ea
15	3.17±0.21 Ea	1.63±0.15 Eb	1.59±0.13 Eb	252.8±17.2Ea	194.9±14.1 Eb	88.1±7.1 Ec	1.01±0.06 Aa	0.78±0.04 Db	0.70±0.05 Eb
30	3.76±0.20 Da	3.21±0.16 Db	3.38±0.18 Db	464.5±28.2 Da	423.4±26.6 Db	342.3±21.5 Dc	0.63±0.04 Cc	0.78±0.05 Db	0.95±0.08 Ca
45	8.84±0.45 Ca	8.14±0.39 Ca	5.13±0.23 Cb	977.5±40.5 Ca	811.4±38.4Cb	688.9±30.3 Cc	0.78±0.05 Bb	0.87±0.06 Ca	0.81±0.06 Dab
60	16.34±0.85 Ba	13.25±0.69 Bb	8.78±0.52Bc	1002.4±64.3 Ba	870.5±54. 9 Bb	808.7±50.5 Bb	0.94±0.07 Ab	1.00±0.09 Bb	1.21±0.11 Ba
75	23.97±1.83 Aa	19.59±1.18 Ab	15.05±1.13 Ac	1214.6±75.4 Aa	941.6±61. 8 Ab	892.7±51.6 Ac	0.97±0.09 Ac	1.35±0.12 Ab	1.99±0.14 Aa

处理时间/d treatment time	叶绿素a含量/(mg·g^-1) chlorophyll a content			叶绿素b含量/(mg·g^-1) chlorophyll b content			类胡萝卜素含量/(mg·g^-1) carotenoid content
处理时间/d treatment time	CK	MN	SN	CK	MN	SN	CK	MN	SN
0	2.62±0.21 Da	2.58±0.23 Aa	2.59±0.24 ABa	1.86±0.12 Ba	1.93±0.15 Aa	1.97±0.14 Ba	0.36±0.03 Ca	0.33±0.03 Ca	0.35±0.03 Ba
15	2.95±0.24 Ca	2.57±0.22 Aa	2.87±0.26 Aa	2.62±0.21 Aa	2.11±0.19 Ab	2.24±0.19 Ab	0.13±0.01 Db	0.27±0.02 Da	0.23±0.02 Ca
30	3.43±0.28 Ba	2.92±0.28 Ab	2.60±0.24 Bc	1.40±0.11 Da	1.16±0.08 Bb	1.01±0.09 Cb	0.97±0.08 Aa	0.83±0.07 Aa	0.64±0.05 Ab
45	2.92±0.25 Ca	2.76±0.26 Ba	2.61±0.23 Ba	1.86±0.15 Ba	0.99±0.06 Cb	0.89±0.07 Db	0.35±0.03 Ca	0.34±0.03 Ca	0.34±0.03 Ba
60	4.08±0.37 Aa	3.32±0.31 Ab	2.79±0.27 Bc	1.62±0.13 Ca	1.27±0.11 Bb	1.15±0.10 Cb	0.54±0.05 Ba	0.44±0.04 bBb	0.39±0.03 Bb
75	3.86±0.34 Aa	2.70±0.25 Bb	1.87±0.17 Cc	1.11±0.10 Ea	0.96±0.08 Cb	0.61±0.07 Ec	0.50±0.04 Ba	0.36±0.03 Cb	0.22±0.02 Cc

不同供氮水平的核桃幼苗生长及叶绿素荧光特性

Growth and chlorophyll fluorescence characteristics of walnut (Juglans regia) seedling under different nitrogen supply levels

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 7

参考文献 29

相关文章 15

编辑推荐

Metrics

本文评价

作者

读者

审稿

[1]	李涌福, 杨庆华, 陈林, 张敏, 向其柏, 王贤荣, 段一凡. 木犀属内分组关系的分类修订[J]. 南京林业大学学报（自然科学版）, 2024, 48(3): 58-62.
[2]	田梦阳, 朱树林, 窦全琴, 季艳红. 薄壳山核桃-茶间作对‘安吉白茶’速生期光合特性的影响[J]. 南京林业大学学报（自然科学版）, 2024, 48(2): 86-96.
[3]	魏静, 谭星, 王昌盛, 闫瑞, 李林珂, 宁月, 刘芸. 引种美国红枫在两种紫色土区的生长和光合特性比较[J]. 南京林业大学学报（自然科学版）, 2024, 48(1): 97-105.
[4]	魏亚娟, 郭靖, 党晓宏, 解云虎, 汪季, 李小乐, 吴慧敏. 吉兰泰荒漠绿洲过渡带不同生境下白刺灌丛沙堆形态特征与影响机制[J]. 南京林业大学学报（自然科学版）, 2023, 47(5): 172-180.
[5]	刘佳磊, 白润娥, 张锴, 文才艺, 闫凤鸣. 我国桂花树上常见粉虱种类记述[J]. 南京林业大学学报（自然科学版）, 2023, 47(5): 237-244.
[6]	邱靖, 李嘉宝, 朱大海, 陈昕. 花楸属直脉组7种/变种基因组大小及叶表皮微形态特征的分类学意义[J]. 南京林业大学学报（自然科学版）, 2023, 47(3): 77-86.
[7]	张赟齐, 董宁光, 郝艳宾, 陈永浩, 张俊佩, 侯智霞, 苏淑钗, 吴佳庆, 齐建勋. 109份丰产核桃单株坚果表型多样性分析及性状评价[J]. 南京林业大学学报（自然科学版）, 2023, 47(3): 87-96.
[8]	孙荣喜, 潘昕昊, 仲小茹, 李桂盛. 不同种源米槠种子形态特征与营养成分变异分析[J]. 南京林业大学学报（自然科学版）, 2023, 47(2): 27-34.
[9]	李建挥, 李柏海, 吴思政, 柏文富, 禹霖, 聂东伶, 严佳文, 熊颖, 向祖恒, 彭先凤. 湘西地区核桃坚果表型特征及多样性研究[J]. 南京林业大学学报（自然科学版）, 2023, 47(1): 171-179.
[10]	唐敏, 杨开宇, 张赛男, 陈利英, 刘洋, 张雪梅, 齐国辉. 硒对核桃种仁抗氧化酶活性及果实品质的影响[J]. 南京林业大学学报（自然科学版）, 2022, 46(5): 127-134.
[11]	贾展慧, 贾晓东, 许梦洋, 莫正海, 翟敏, 宣继萍, 张计育, 王刚, 王涛, 郭忠仁. 薄壳山核桃原花青素合成关键酶基因的克隆与表达分析[J]. 南京林业大学学报（自然科学版）, 2022, 46(5): 49-57.
[12]	田梦阳, 窦全琴, 谢寅峰, 汤文华, 季艳红. 4个薄壳山核桃品种的光合特性研究[J]. 南京林业大学学报（自然科学版）, 2022, 46(5): 67-74.
[13]	季琳琳, 陈素传, 吴志辉, 常君, 陶汝鹏, 周米生, 蔡新玲. 山核桃果实主要经济性状和养分含量的差异分析[J]. 南京林业大学学报（自然科学版）, 2022, 46(1): 131-137.
[14]	季艳红, 潘平平, 窦全琴, 谢寅峰. 不同泥炭替代基质对薄壳山核桃幼苗生长及叶绿素荧光特性的影响[J]. 南京林业大学学报（自然科学版）, 2022, 46(1): 145-155.
[15]	季艳红, 汤文华, 窦全琴, 谢寅峰. 施肥对薄壳山核桃容器苗生长及养分积累的影响[J]. 南京林业大学学报（自然科学版）, 2021, 45(6): 47-56.