凉山油橄榄主栽品种叶中营养物质和酚类含量的季节性变化

doi:10.12302/j.issn.1000-2006.202105020

国家林草科技领军期刊
中国精品科技期刊
中国高校百佳科技期刊
江苏省新闻出版政府奖期刊奖
RCCSE林学权威期刊（A+）
CSCD核心期刊
Scopus数据库收录期刊
中文核心期刊
SCD核心期刊

作者加群：102861116

微信公众号：南京林业大学学报

高级检索

南京林业大学学报（自然科学版） ›› 2022, Vol. 46 ›› Issue (4): 169-176.doi: 10.12302/j.issn.1000-2006.202105020

凉山油橄榄主栽品种叶中营养物质和酚类含量的季节性变化

王碧霞¹(), 杜小奇¹, 邓燕¹, 蔡晓梅¹, 苏光灿²

1.西华师范大学环境科学与工程学院,四川南充 637002
2.凉山州中泽新技术开发有限责任公司,四川西昌 615000

收稿日期:2021-05-11 修回日期:2021-08-25 出版日期:2022-07-30 发布日期:2022-08-01
基金资助:
西华师范大学博士科研启动基金项目(20E046);四川省南充市科技局项目(19YFZJ0108);四川省教育厅科研项目(SDJJ1915)

Seasonal variations of the nutrients and phenols from olive leaves in main cultivation varieties at Liangshan, Sichuan Province

WANG Bixia¹(), DU Xiaoqi¹, DENG Yan¹, CAI Xiaomei¹, SU Guangcan²

1. College of Environmental Science and Engineering, China West Normal University, Nanchong 637002, China
2. Liangshan Zhongze New Technology Development Co., Ltd, Xichang 615000, China

Received:2021-05-11 Revised:2021-08-25 Online:2022-07-30 Published:2022-08-01

摘要/Abstract

摘要：

【目的】油橄榄叶是油橄榄产业发展过程中产生的农林废弃物,研究不同品种及叶龄油橄榄叶中主要营养物质和酚类含量在不同季节的变化,为油橄榄叶在药品、保健品和功能性食品等方面的高值化应用提供理论依据和技术支撑。【方法】以四川凉山6个油橄榄主栽品种的老叶和嫩叶为研究对象,检测其游离氨基酸(FAAC)、可溶性糖(SSC)和可溶性蛋白(SPC)等营养物质含量差异,并通过高效液相色谱法(HPLC)分析7种主要酚类成分(橄榄苦苷、芦丁、木犀草素-4'-O-葡萄糖苷、芹菜素-7-O-葡萄糖苷、木犀草素、槲皮素和芹菜素)含量的季节变化。【结果】凉山地区油橄榄老叶和嫩叶中主要营养物质和酚类成分呈季节性不均匀分布状态,7种酚类成分在夏秋季节波动幅度大,FAAC、SSC、SPC含量在2—3月和5—6月相对较高;6个品种中,‘鄂植’与‘卡林’叶中营养物质和酚类成分比其他品种丰富,且嫩叶中其含量水平比老叶的高。【结论】油橄榄叶中营养物质丰富和酚类成分稳定的最佳季节为春季,‘鄂植’和‘卡林’嫩叶可作为潜在的高值化应用品种材料来源加以开发和利用。

关键词: 油橄榄叶, 酚类成分, 营养物质, 季节变化, 四川凉山

Abstract:

【Objective】 Olive (Olea europaea) leaves are an agricultural and forestry waste in the olive farming industry. This study aimed to determine their main nutrients and phenolic components, and analyze the content changes between the old and young leaves among olive varieties from January to December. The results can lay a foundation and provide technical support for the high-value application of olive leaves in medicines, health products, and functional foods, etc. 【Method】 Among the six main olive cultivars harvested in Liangshan, Sichuan Province, China, differences in the concentration of free amino acids (FAAC), soluble sugars (SSC), and soluble proteins (SPC) between the old and young leaves were investigated, and level changes in seven main phenolic compounds (oleuropein, rutin, luteolin-4'-O-glucoside, apigenin-7-O-glucoside, luteolin, quercetin and apigenin) from January to December were evaluated using high performance liquid chromatography (HPLC). 【Result】 The nutrients and phenols of the old and young leaves among the six olive cultivars were unevenly distributed during the year. The phenolic compounds fluctuated greatly in summer and autumn. FAAC, SSC and SPC exhibited relatively high levels approximately at 2-3 and 5-6 months. Compared with the other varieties, the leaves of ‘Ezhi’ and ‘Kalinjot’ cultivars had of nutrients and phenolic components, with the young leaves possessing higher concentration than the old leaves. 【Conclusion】 The most suitable season to obtain nutrients and phenols from the olive leaves was spring, especially in March, and the best choice was the young leaves of ‘Ezhi’ and ‘Kalinjot’, which could be exploited and utilized reasonably as a potential source.

Key words: olive leaves, phenolic compounds, nutrients, seasonal variation, Liangshan,Sichuan Province

中图分类号:

S789
S718

王碧霞,杜小奇,邓燕,等. 凉山油橄榄主栽品种叶中营养物质和酚类含量的季节性变化[J]. 南京林业大学学报（自然科学版）, 2022, 46(4): 169-176.

WANG Bixia, DU Xiaoqi, DENG Yan, CAI Xiaomei, SU Guangcan. Seasonal variations of the nutrients and phenols from olive leaves in main cultivation varieties at Liangshan, Sichuan Province[J].Journal of Nanjing Forestry University (Natural Science Edition), 2022, 46(4): 169-176.DOI: 10.12302/j.issn.1000-2006.202105020.

图/表 7

图1

图2

表1

表2

The analysis of main phenolic contents for the seasons and leaf ages from olive leaves单位:mg/g"

月份 month	叶龄 leaf age	橄榄苦苷 oleuropein	芦丁 rutin	木犀草素-4'- O-葡萄糖苷 luteolin-4'-O- glucoside	芹菜素-7-O- 葡萄糖苷 apigenin-7-O- glucoside	木犀草素 luteolin	槲皮素 quercetin
1	老叶OL	43.41±4.49 i	3.65±0.40 a	28.77±3.12 efgh	1.18±0.12 e	0.016±0.001 0 cd	0.050±0.004 7 de
	嫩叶YL	50.64±0.74 hi	3.82±0.23 a	37.73±2.29 cd	1.85±0.05 cd	0.020±0.001 9 cd	0.059±0.002 6 cd
2	老叶OL	24.35±0.75 kl	1.08±0.05 ef	28.45±1.17 efgh	1.19±0.04 e	0.003±0.000 3 d	0.056±0.003 0 cd
	嫩叶YL	66.36±3.74 ef	0.33±0.02 hijk	42.43±1.93 bc	2.48±0.10 b	0.004±0.000 6 d	0.044±0.007 4 defg
3	老叶OL	43.25±0.04 i	0.31±0.01 hijk	27.44±7.06 fgh	2.30±0.05 b	0.004±0.000 3 d	0.025±0.000 9 efgh
	嫩叶YL	116.87±6.60 a	0.23±0.05 ijk	31.63±1.62 defg	1.77±0.14 cd	0.008±0.000 3 d	0.191±0.015 1 a
4	老叶OL	59.52±3.27 fg	0.01±0.01 k	1.41±0.27 l	0.41±0.04 h	0.028±0.003 5 c	nd
	嫩叶YL	19.59±0.04 l	0.05±0.01 jk	1.86±0.18 l	0.57±0.06 gh	0.019±0.001 7 cd	nd
5	老叶OL	87.16±1.37 c	0.45±0.01 hi	33.58±0.38 def	2.36±0.0 4b	0.013±0.001 0 cd	nd
	嫩叶YL	43.23±0.79 i	0.36±0.04 hijk	27.93±3.02 efgh	1.82±0.19 cd	0.071±0.019 9 b	nd
6	老叶OL	33.98±0.05 j	1.50±0.01 cd	44.58±0.43 abc	3.06±0.04 a	0.012±0.000 5 cd	0.025±0.000 5 efgh
	嫩叶YL	98.67±5.39 b	0.83±0.035 fg	47.76±0.13 ab	2.44±0.04 b	0.010±0.000 3 cd	0.134±0.016 6 b
7	老叶OL	20.77±0.13 l	0.49±0.01 ghi	35.20±0.77 de	1.89±0.05 c	0.005±0.000 6 d	0.123±0.015 0 b
	嫩叶YL	58.02±0.33 g	0.43±0.06 hij	22.26±3.32 hij	1.54±0.22 d	0.007±0.000 5 d	0.004±0.000 7 h
8	老叶OL	23.94±0.11 kl	0.61±0.02 ghi	30.71±0.62 defg	2.18±0.0 4b	0.010±0.001 0 cd	0.029±0.002 5 e fgh
	嫩叶YL	55.85±0.41 gh	0.44±0.01 hi	33.29±0.29 def	1.78±0.03 cd	nd	0.114±0.001 5 b
9	老叶OL	6.15±0.08 m	0.64±0.03 gh	12.28±0.42 k	0.77±0.03 fg	0.004±0.000 1 d	nd
	嫩叶YL	5.82±0.01 m	0.66±0.03 gh	17.49±0.72 jk	1.10±0.04 e	0.008±0.000 7 cd	nd
10	老叶OL	58.55±0.12 g	1.21±0.11 de	11.77±1.29 k	0.73±0.07 fg	0.003±0.000 3 d	0.019±0.002 2 gh
	嫩叶YL	76.43±3.29 d	1.36±0.26 de	19.87±4.69 ij	0.97±0.22 ef	0.002±0.000 1 d	0.049±0.007 0 def
11	老叶OL	29.27±0.25 jk	1.75±0.04 c	18.52±0.50 ijk	2.38±0.05 b	0.020±0.001 0 cd	0.022±0.000 5 fgh
	嫩叶YL	72.26±0.25 de	2.45±0.06 b	51.19±1.12 a	3.19±0.06 a	0.005±0.000 3 d	nd
12	老叶OL	4.44±0.21 m	1.29±0.08 de	25.59±1.43 ghi	1.60±0.08 cd	0.118±0.008 0 a	0.033±0.002 5 defg
	嫩叶YL	6.24±0.13 m	0.84±0.04 fg	21.98±1.26 hij	1.25±0.01 e	nd	0.082±0.002 5 c

表2

表3

表4

表5

参考文献 24

[1]	MEDINA E, ROMERO C, GARCÍA P, et al. Characterization of bioactive compounds in commercial olive leaf extracts,and olive leaves and their infusions[J]. Food Funct, 2019, 10(8):4716-4724.DOI:10.1039/C9FO00698B.
[2]	WU Z Q, YUE G Z, ZHU Q P, et al. Purification,dynamic changes and antioxidant activities of oleuropein in olive (Olea europaea L.) leaves[J]. J Food Biochem, 2015, 39(5):566-574.DOI:10.1111/jfbc.12152.
[3]	SU C J, SUN J F, ZHU W Z, et al. History,distribution,and potential of the olive industry in China:a review[J]. Sustainability, 2018, 10(5):1426.DOI:10.3390/su10051426.
[4]	CHENG ZZ, ZHAN M M, YANG Z S, et al. The major qualitative characteristics of olive (Olea europaea L.) cultivated in southwest China[J]. Front Plant Sci, 2017, 8:559.DOI:10.3389/fpls.2017.00559.
[5]	ÖZCAN M M, MATTHÄUS B. A review: benefit and bioactive properties of olive (Olea europaea L.) leaves[J]. Eur Food Res Technol, 2017, 243(1):89-99.DOI:10.1007/s00217-016-2726-9.
[6]	刘红梅, 方成鑫, 郭力, 等. 不同采收季节对油橄榄茶品质的影响[J]. 保鲜与加工, 2019, 19(1):127-132.
	LIU H M, FANG C X, GUO L, et al. Effect of different harvest seasons on quality of olive tea[J]. Storage Process, 2019, 19(1):127-132.DOI:10.3969/j.issn.1009-6221.2019.01.022.
[7]	ACAR-TEK N, AGA ĞÜNDÜZ D. Olive leaf (Olea europaea L. Folium):potential effects on glycemia and lipidemia[J]. Ann Nutr Metab, 2020, 76(1):10-15.DOI:10.1159/000505508.
[8]	GHASEMI S, KOOHI D E, EMMAMZADEHHASHEMI M S B, et al. Investigation of phenolic compounds and antioxidant activity of leaves extracts from seventeen cultivars of Iranian olive (Olea europaea L.)[J]. J Food Sci Technol, 2018, 55(11):4600-4607.DOI:10.1007/s13197-018-3398-1.
[9]	DEMIR S, CETINKAYA H. Effects of saline conditions on polyphenol and protein content and photosynthetic response of different olive (Olea europaea L.) cultivars[J]. Appl Ecol Env Res, 2020, 18(2):2599-2610.DOI:10.15666/aeer/1802_25992610.
[10]	PASKOVIĆ I, LUKIĆ I, ŽURGA P, et al. Temporal variation of phenolic and mineral composition in olive leaves is cultivar dependent[J]. Plants (Basel), 2021, 9(9):1099.DOI:10.3390/plants9091099.
[11]	DIAB M A, IBRAHIM A K, HADAD G M, et al. Seasonal variations in antioxidant components of Olea europaea in leaves of different cultivars,seasons,and oil products in Sinai[J]. Food Anal Methods, 2021, 14(4):773-783.DOI:10.1007/s12161-020-01919-9.
[12]	WANG B X, QU J P, FENG S L, et al. Seasonal variations in the chemical composition of Liangshan olive leaves and their antioxidant and anticancer activities[J]. Foods, 2019, 8(12):657.DOI:10.3390/foods8120657.
[13]	RANALLI A, CONTENTO S, LUCERA L, et al. Factors affecting the contents of iridoid oleuropein in olive leaves (Olea europaea L.)[J]. J Agric Food Chem, 2006, 54(2):434-440.DOI:10.1021/jf051647b.
[14]	TALHAOUI N, TAAMALLI A, GÓMEZ-CARAVACA A M, et al. Phenolic compounds in olive leaves: analytical determination, biotic and abiotic influence,and health benefits[J]. Food Res Int, 2015, 77:92-108.DOI:10.1016/j.foodres.2015.09.011.
[15]	WILSON R P. Amino acids and proteins[M]// ROBERT P W. Fish nutrition. Amsterdam: Elsevier, 2003: 143-179.
[16]	BUYSSE J, MERCKX R. An improved colorimetric method to quantify sugar content of plant tissue[J]. J Exp Bot, 1993, 44(10):1627-1629.DOI:10.1093/jxb/44.10.1627.
[17]	MEDA A, LAMIEN C E, ROMITO M, et al. Determination of the total phenolic,flavonoid and proline contents in Burkina Fasan honey,as well as their radical scavenging activity[J]. Food Chem, 2005, 91(3):571-577.DOI:10.1016/j.foodchem.2004.10.006.
[18]	JAPÓN-LUJÁN R, LUQUE-RODRÍGUEZ J M, LUQUE DE CASTRO M D. Dynamic ultrasound-assisted extraction of oleuropein and related biophenols from olive leaves[J]. J Chromatogr A, 2006, 1108(1):76-82.DOI:10.1016/j.chroma.2005.12.106.
[19]	SALAH M, ABDELMELEK H, ABDERRABBA M. Study of phenolic composition and biological activities assessment of olive leaves from different varieties grown in Tunisia[J]. Med Chem (Los Angeles), 2012, 2(5):107-111.DOI:10.4172/2161-0444.1000124.
[20]	SAVOURNIN C, BAGHDIKIAN B, ELIAS R, et al. Rapid high-performance liquid chromatography analysis for the quantitative determination of oleuropein in Olea europaea leaves[J]. J Agric Food Chem, 2001, 49(2):618-621.DOI:10.1021/jf000596+.
[21]	TALHAOUI N, GÓMEZ-CARAVACA A M, ROLDÁN C, et al. Chemometric analysis for the evaluation of phenolic patterns in olive leaves from six cultivars at different growth stages[J]. J Agric Food Chem, 2015, 63(6):1722-1729.DOI:10.1021/jf5058205.
[22]	姜东, 于振文, 李永庚, 等. 冬小麦叶茎粒可溶性糖含量变化及其与籽粒淀粉积累的关系[J]. 麦类作物学报, 2001, 21(3):38-41.
	JIANG D, YU Z W, LI Y G, et al. Changes of soluble sugar contents in leaf,stem and grain in winter wheat and its relationship with grain starch accumulation[J]. Acta Tritical Crops, 2001, 21(3):38-41.
[23]	杨敏, 李隆云, 杨水平, 等. 青蒿生育期可溶性糖和氨基酸含量动态变化[J]. 时珍国医国药, 2009, 20(2):260-261.
	YANG M, LI L Y, YANG S P, et al. Dynamic change of soluble saccharides and amino acids during the growing period of Artemisia annua L[J]. Lishizhen Med Mater Med Res, 2009, 20(2):260-261.DOI:10.3969/j.issn.1008-0805.2009.02.002.
[24]	陈仕江, 钟国跃, 徐金辉. 黄连生育期间可溶性糖和氨基酸含量动态的研究[J]. 中国中药杂志, 2005, 30(17):1324-1327.
	CHEN S J, ZHONG G Y, XU J H. Dynamic change of soluble saccharides and amino acids during the growing period of Coptis chinensis[J]. China J Chin Mater Med, 2005, 30(17):1324-1327.DOI:10.3321/j.issn:1001-5302.2005.17.005.

成分 compound	校准方程 calibration equition	R²	校准范围/ (mg·mL^-1) linear calibration range
橄榄苦苷oleuropein	y=687.63 x-10.877 0	0.999 1	0~2.000 0
芦丁rutin	y=1 915.30 x+2.606 3	0.998 8	0~0.200 0
木犀草素-4'-O-葡萄糖苷 luteolin-4'-O-glucoside	y=256.15 x+7.045 5	0.998 1	0~2.500 0
芹菜素-7-O-葡萄糖苷 apigenin-7-O-glucoside	y=2 745.80 x+0.129 9	0.999 9	0~0.250 0
木犀草素luteolin	y=3 157.00 x-5.031 8	0.999 7	0~0.150 0
槲皮素quercetin	y=378 228.00 x-1.012 1	0.998 9	0~0.000 4
芹菜素apigenin	y=4 910.80 x-3.607 1	0.999 1	0~-0.025 0

品种 cltuviars	叶龄 leaf age	橄榄苦苷 oleuropein	芦丁 rutin	木犀草素-4'-O- 葡萄糖苷 luteolin-4'-O- glucoside	芹菜素-7-O- 葡萄糖苷 apigenin-7-O- glucoside	木犀草素 luteolin	槲皮素 quercetin	芹菜素 apigenin
‘皮削利’ ‘Picholine’	老叶OL	28.12±0.40 g	2.78±0.08 b	42.89±1.44 b	2.30±0.09 cd	0.003±0.001 f	0.007±0.002 5 ef	nd
	嫩叶YL	62.34±0.76 d	1.46±0.08 def	25.55±2.09 cd	2.06±0.13 d	0.022±0.003 b	0.010±0.003 5 a	nd
‘科拉蒂’ ‘Coratina’	老叶OL	34.07±0.29 f	2.11±0.02 bcd	49.99±0.50 a	2.39±0.03 bc	0.004±0.001 ef	0.002±0.000 3 f	nd
	嫩叶YL	65.40±1.42 cd	3.71±0.32 a	52.57±2.62 a	2.69±0.13 a	0.011±0.001 cde	0.014±0.005 5 ef	nd
‘阿斯’ ‘Ascolana tenera’	老叶OL	43.62±0.21 e	0.75±0.11f g	26.13±1.30 cd	1.55±0.14 efg	0.015±0.001 bc	0.018±0.000 1 e	0.043±0.004 ab
	嫩叶YL	40.74±0.12 e	0.53±0.02 g	29.51±1.41 c	2.58±0.10 ab	0.013±0.001 cd	0.007±0.000 5 ef	nd
‘鄂植’ ‘Ezhi’	老叶OL	27.30±3.63 gh	2.52±0.29 bc	49.94±3.65 a	2.07±0.01 d	0.009±0.003 def	0.007±0.002 0 ef	0.0120±0.009c
	嫩叶YL	23.83±0.09 h	4.38±0.59 a	40.12±1.32 b	1.39±0.01 efg	0.078±0.008 a	0.075±0.005 5 b	0.059±0.002 a
‘卡林’ ‘Kalinjot’	老叶OL	43.42±0.10 e	2.16±0.14 bcd	30.24±1.51 c	1.79±0.03 e	0.014±0.001 bc	0.017±0.002 0 e	nd
	嫩叶YL	94.38±0.73 a	4.02±0.12 a	29.83±1.25 c	1.64±0.01 ef	0.018±0.002 bc	0.061±0.010 0 c	0.025±0.003 bc
‘戈达尔’ ‘Gordal’	老叶OL	66.32±0.45 c	1.35±0.05 ef	38.15±2.79 b	1.60±0.04 ef	0.007±0.001 def	0.015±0.000 1 e	nd
	嫩叶YL	85.56±0.67 b	1.95±0.19 cde	20.63±0.65 d	1.29±0.02 g	0.015±0.001 bc	0.040±0.003 5 d	nd

月份 months	游离氨基酸FAAC		可溶性糖SSC		可溶性蛋白SPC
月份 months	老叶OL	嫩叶YL	老叶OL	嫩叶YL	老叶OL	嫩叶YL
1	18.57±0.64 b	12.06±0.96 def	12.89±0.19 e	16.07±0.58 d	3.29±0.14 cdefgh	3.57±0.77 bcdefg
2	17.67±0.56 b	27.25±1.31 a	9.40±0.36 gh	12.07±0.29 de	3.85±0.25 bcde	4.07±0.27 bcd
3	14.07±1.04 cd	16.16±2.14 bc	15.76±0.29 d	18.83±0.50 b	2.76±0.19 efghi	4.62±0.22 b
4	3.43±0.07 jk	2.92±0.12 k	9.08±0.28 h	8.89±0.59 h	1.43±0.06 j	2.11±0.56 hij
5	7.83±0.91 hi	8.69±0.69 gh	15.50±0.76 d	16.68±0.47 cd	5.97±0.79 a	2.87±0.21 defgh
6	5.70±0.39 ij	26.80±2.17 a	17.86±0.81 bc	21.23±1.30 a	2.34±0.41 hij	4.44±0.50 bc
7	8.70±0.25 gh	15.92±1.11 bc	7.30±0.05 i	4.11±0.77 j	2.78±0.31 efghi	2.55±0.24 fghij
8	7.72±0.58 hi	8.64±0.76 gh	11.91±0.77 de	10.09±0.37 fgh	2.49±0.37 ghij	3.05±0.35 defgh
9	11.27±0.52 efg	13.62±0.62 cde	4.28±0.07 j	4.33±0.59 j	2.09±0.09 hij	1.64±0.15 ij
10	5.07±0.27 jk	4.58±0.20 jk	6.89±0.35 i	11.02±0.45 efg	2.17±0.27 hij	3.57±0.36 bcdefg
11	8.74±0.61 gh	9.49±0.91 fgh	5.73±0.48 ij	11.21±0.42 def	2.17±0.15 hij	3.34±0.27 cdefgh
12	18.57±0.64 b	26.36±0.62 a	5.13±0.19 j	4.43±0.28 j	4.01±0.25 bcd	3.74±0.26 bcdef

品种 cultivars	游离氨基酸FAAC		可溶性糖SSC		可溶性蛋白SPC
品种 cultivars	老叶OL	嫩叶YL	老叶OL	嫩叶YL	老叶OL	嫩叶YL
‘皮削利’‘Picholine’	19.33±0.05 f	25.95±2.47 e	9.28±0.44 e	15.87±0.76 bc	5.66±0.11 f	6.89±0.07 bcd
‘科拉蒂’‘Coratina’	27.80±1.94 de	33.37±2.32 c	15.40±0.23 bc	17.45±0.94 b	6.72±0.53 cde	5.89±0.08 ef
‘阿斯’‘Ascolana tenera’	18.94±0.98 f	34.23±1.71 c	12.24±0.45 d	5.86±0.05 f	6.80±0.05 bcd	7.67±0.35 ab
‘鄂植’‘Ezhi’	30.56±0.32 cde	32.34±0.11 cd	16.09±0.77 bc	15.63±0.36 bc	7.33±0.06 abc	6.12±0.14 def
‘卡林’‘Kalinjot’	31.15±0.53 cde	42.64±2.24 b	9.71±0.20 e	21.54±0.75 a	6.18±0.42 def	8.15±0.45 a
‘戈达尔’‘Gordal’	33.45±0.66 c	47.67±3.13 a	14.10±0.79 cd	8.34±0.48 e	7.10±0.02 bc	7.34±0.09 abc

凉山油橄榄主栽品种叶中营养物质和酚类含量的季节性变化

Seasonal variations of the nutrients and phenols from olive leaves in main cultivation varieties at Liangshan, Sichuan Province

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 7

参考文献 24

相关文章 15

编辑推荐

Metrics

本文评价

作者

读者

审稿

[1]	宫楠, 祖鑫, 解志军, 朱长红, 李淑娴. 紫荆种子吸胀和层积过程中不同相态水分变化的核磁共振检测[J]. 南京林业大学学报（自然科学版）, 2023, 47(6): 42-50.
[2]	刘倩倩, 彭孝楠, 刘鑫, 王舒甜, 戴康龙, 徐海兵, 董丽娜, 张金池. 踩踏干扰下紫金山土壤质量季节变化特征[J]. 南京林业大学学报（自然科学版）, 2022, 46(3): 185-193.
[3]	邹雨婷, 朱铭玮, 李永荣, 翟金庭, 李淑娴. ‘凤丹’种子发育及其营养物质含量和相关酶活性的动态变化[J]. 南京林业大学学报（自然科学版）, 2021, 45(5): 62-70.
[4]	俞琳琳, 胡海波, 余伟. 城市绿地类型对大气PM_2.5浓度的影响[J]. 南京林业大学学报（自然科学版）, 2020, 44(3): 179-184.
[5]	魏黔春, 江泽平, 刘建锋, 史胜青, 赵秀莲, 常二梅. 侧柏古树扦插试验及插穗营养物质变化[J]. 南京林业大学学报（自然科学版）, 2020, 44(1): 63-71.
[6]	张悦, 冯会丽, 王维枫, 薛建辉, 吴永波, 于水强. 洪泽湖地区杨树人工林碳水通量昼夜和季节变化特征[J]. 南京林业大学学报（自然科学版）, 2019, 43(5): 113-120.
[7]	蒋婵,李青青,刘莉莉,周杰珑,陈粉粉,杨亚晋,张璇,郭爱伟. 大熊猫10种潜在食竹营养成分的季节变化[J]. 南京林业大学学报（自然科学版）, 2018, 61(05): 148-154.
[8]	张丹丹,范俊俊,赵明明,蔡喜彦,张往祥. 外施植物生长调节剂对细柄阿丁枫扦插生根的影响[J]. 南京林业大学学报（自然科学版）, 2018, 61(01): 41-47.
[9]	张文文,肖晗冉,杨宝玲,董可嘉,阮宏华,郑阿宝,沈彩芹,曹国华. 不同土地利用类型土壤动物对土壤氮矿化季节变化的影响[J]. 南京林业大学学报（自然科学版）, 2016, 59(06): 20-26.
[10]	许文欢,邓芳芳,方水元,王国兵,阮宏华,曹国华. 生物炭对杨树人工林土壤微生物量及碳源代谢多样性的影响[J]. 南京林业大学学报（自然科学版）, 2016, 59(05): 14-20.
[11]	吴雅琼,汪贵斌,曹福亮,祖元刚. 基质、插穗及促根剂对喜树扦插生根的影响[J]. 南京林业大学学报（自然科学版）, 2016, 59(03): 1-8.
[12]	王艮梅,罗琳琳,郑聚锋. 苏北不同代次和林龄杨树人工林土壤酶活性季节变化特征[J]. 南京林业大学学报（自然科学版）, 2014, 57(04): 45-50.
[13]	许凯,徐钰,葛之葳,于水强,王国兵,范换,周丹燕,阮宏华. 氮添加对杨树人工林土壤活性有机碳季节变化的影响[J]. 南京林业大学学报（自然科学版）, 2014, 57(03): 19-23.
[14]	杨秀莲，王良桂. 桂花种子对赤霉素处理的生理生化响应[J]. 南京林业大学学报（自然科学版）, 2012, 55(01): 63-67.
[15]	吴春林,,陶忠芳,,王国兵,阮宏华. 次生栎林与火炬松人工林的土壤微生物量氮动态[J]. 南京林业大学学报（自然科学版）, 2010, 53(06): 38-42.