彩叶桂叶片发育过程中叶色表型与色素成分变化

doi:10.12302/j.issn.1000-2006.202109057

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南京林业大学学报（自然科学版） ›› 2023, Vol. 47 ›› Issue (2): 79-86.doi: 10.12302/j.issn.1000-2006.202109057

彩叶桂叶片发育过程中叶色表型与色素成分变化

崔祺¹(), 吴昀¹, 李东泽¹^,^*(), 吴凡¹, 韩蕊莲¹^,², 黄均华¹, 胡绍庆¹

1.浙江理工大学建筑工程学院,浙江杭州 310018
2.西北农林科技大学草业与草原学院, 陕西杨凌 712100

收稿日期:2021-09-28 接受日期:2021-12-05 出版日期:2023-03-30 发布日期:2023-03-28
通讯作者: * 李东泽(dongzeli06@163.com),讲师。
基金资助:
浙江省自然科学基金项目(LQ20C150003);浙江省一流学科(土木工程)建设项目(11141131282001)

Changes of coloration and pigment compositions during leaf development of Osmanthus fragrans colour group cultivar

CUI Qi¹(), WU Yun¹, LI Dongze¹^,^*(), WU Fan¹, HAN Ruilian¹^,², HUANG Junhua¹, HU Shaoqing¹

1. School of Civil Engineering and Architecture, Zhejiang Sci-Tech University, Hangzhou 310018, China
2. College of Grassland Agriculture, Northwest A & F University, Yangling 712100, China

Received:2021-09-28 Accepted:2021-12-05 Online:2023-03-30 Published:2023-03-28

摘要/Abstract

摘要：

【目的】探究彩叶桂叶片发育过程中叶色表型与色素成分的关系。【方法】以彩叶桂品种‘虔南桂妃’(Osmanthus fragrans ‘Qiannan Guifei’)5个发育时期(S1—S5)的叶片为研究对象,选用英国皇家园艺学会比色卡和色差仪测量其叶色表型,同时采用可见光分光光度法对5个时期的叶片进行叶绿素和类胡萝卜素的测定,利用超高效液相色谱-串联三重四级杆-飞行时间质谱(UPLC-Triple-TOF/MS)联用技术定性定量分析叶片中类黄酮成分与含量,进一步分析了叶色与各色素成分之间的相关性。【结果】随着叶片不断发育,彩叶桂叶片颜色变化大致分为两个阶段:第1个阶段由红转黄(S1—S3),第2个阶段由黄转绿(S3—S5)。叶片颜色参数明度L^*值和蓝度b^*值先增加后下降,而红a^*值和彩度C^*值则呈下降趋势。在叶片中共鉴定出了8种类黄酮,包括1种花青素(Cy3R)、3种黄酮(Ap7G、Ap7R和vicenin-2)以及4种黄酮醇(Km3R、Km7G、Km3G7R、Qu3R)。定量分析发现:叶绿素和类胡萝卜素在S1—S3时期含量较低且较为稳定,在S3—S5时期二者含量及其比值呈显著增加的趋势;Cy3R的含量在S1—S3时期显著降低,在S3—S5时期含量极低,未检测出;Qu3R、Km3R、Km7G和Ap7G含量从S1—S5显著降低,而vicenin-2和Km3G7R含量则呈现出持续增加的趋势;Ap7R的含量在S1—S3时期较为稳定,之后先下降后增加。【结论】彩叶桂‘虔南桂妃’叶色由红转黄主要是因为花青素(Cy3R)、黄酮(Ap7G)和黄酮醇(Qu3R、Km3R和Km7G)含量的降低,由黄转绿则是因为叶绿素和类胡萝卜素含量的比值增加。

关键词: 彩叶桂, 叶色, 叶绿素, 类黄酮, 花青素, ‘虔南桂妃’

Abstract:

【Objective】 This study aimed to explore the relationship between leaf color changes and pigment composition in Osmanthus fragrans color group during the leaf development. 【Method】 Different-colored leaves of the O. fragrans color group cultivar ‘Qiannan Guifei’ were selected from five developmental stages (S1-S5) for phenotype mensuration using the Royal Horticultural Society Color Chart and colorimeter. Chlorophyll and carotenoid concentrations of the leaves were determined using visible light spectrophotometry. Flavonoids were qualitatively and quantitatively characterized using ultra-performance liquid chromatography triple-time-of-flight/mass spectrometry (UPLC-Triple-TOF/MS). The correlation between leaf color and pigment components was also analyzed. 【Result】 The results indicated that the changes in leaf color could be roughly divided into two stages in relation to the leaf development: the first stage was from red to yellow (S1-S3), and the second stage was from yellow to green (S3-S5). The color parameters L^* and b^* initially increased and then decreased, while the values of a^* and C^* decreased. Eight flavonoids were detected in the leaves of ‘Qiannan Guifei’, including one anthocyanidin (Cy3R), three flavones (Ap7G, Ap7R and vicenin-2), and four flavonols (Km3R, Km7G, Km3G7R and Qu3R). The quantitative analysis showed that chlorophyll and carotenoid concentrations were relatively low and stable during stages S1-S3, whereas the concentrations and ratios of the two pigments increased significantly during stages S3-S5. Cy3R concentration significantly decreased from stages S1 to stage S3, and it occurred in undetectable trace amounts in stages S3-S5. The concentrations of Qu3R, Km3R, Km7G and Ap7G also decreased from stage S1 to stage S5, while vicenin-2 and Km3G7R showed a continuous increase. Ap7R was stable from stage S1 to stage S3, and then it initially decreased and then increased. 【Conclusion】 The decrease of anthocyanidin (Cy3R), flavone (Ap7G) and flavonols (Qu3R, Km3R and Km7G) was critical in promoting changes in leaf color from red to yellow in ‘Qiannan Guifei’, while the increase in the ratio of chlorophylls to carotenoids resulted in the leaves changing from yellow to green.

Key words: Osmanthus fragrans colour group, leaf color, chlorophyll, flavonoid, anthocyanin, ‘Qiannan Guifei’

中图分类号:

S685.13

崔祺,吴昀,李东泽,等. 彩叶桂叶片发育过程中叶色表型与色素成分变化[J]. 南京林业大学学报（自然科学版）, 2023, 47(2): 79-86.

CUI Qi, WU Yun, LI Dongze, WU Fan, HAN Ruilian, HUANG Junhua, HU Shaoqing. Changes of coloration and pigment compositions during leaf development of Osmanthus fragrans colour group cultivar[J].Journal of Nanjing Forestry University (Natural Science Edition), 2023, 47(2): 79-86.DOI: 10.12302/j.issn.1000-2006.202109057.

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时期 stage	比色卡色号 RHSCC No.	L^*	a^*	b^*	C^*
S1	34A	38.77± 2.30 d	28.89± 1.68 a	10.10± 1.77 d	30.64± 1.84 a
S2	170D	53.20± 2.28 c	15.62± 1.19 b	17.63± 1.60 c	23.60± 1.32 b
S3	2D	68.70± 1.69 a	0.16± 1.47 c	29.82± 1.08 a	29.85± 1.10 a
S4	145C	55.00± 2.38 b	-7.61± 2.28 d	24.67± 1.91 b	25.90± 1.99 b
S5	137D	35.06± 1.28 e	-7.90± 0.72 d	16.47± 1.29 c	18.28± 1.31 c

成分编号 No. of component	保留时间/ min retention time	准分子离子 (m/z) molecular ions	碎片离子 (m/z) fragment ions	推定物质 tentative compound	推定依据 constructive basis
P1	6.06	595	287、449、596	矢车菊素-3-O-芸香糖苷 cyaindin-3-O-rutinoside, Cy3R	参考文献[20]
P2	4.30	354	191、353	绿原酸 chlorogenic acid	Scifinder和Reaxy数据库
P3	5.86	594	353、383、413、 473、503、593	葡萄糖基芹菜素 vicenin-2	Scifinder和Reaxy数据库; 参考文献[21]
P4	6.11	368	93、134、173、 191、193、367	阿魏酰奎尼酸 5-feruloylquinic acid	参考文献[22]
P5	7.50	624	623、461、315	阿克苷 acteoside	Scifinder和Reaxy数据库; 参考文献[23]
P6	7.79	610	609、301	斛皮素-3-O-芸香糖苷 quercetin-3-O-rutinoside, Qu3R	参考文献[24]
P7	8.07	448	447、285	山柰酚-7-O-葡萄糖苷 kaempferol-7-O-glucoside, Km7G	参考文献[25]
P8	8.38	594	593、447、285	山柰酚-3-O-葡萄糖基-7-O-鼠李糖苷 kaempferol-3-O-glucopyranosyl-7-O- rhamnopyranoside, Km3G7R	参考文献[26]
P9	8.71	594	593、285	山柰酚-3-O-芸香糖苷 kaempferol-3-rutinoside,Km3R	参考文献[27]
P10	9.06	432	431、269	芹菜素-7-O-葡萄糖苷 apigenin 7-O-glucoside, Ap7G	参考文献[28]
P11	9.22	578	577、269	芹菜素-7-O-芸香糖苷 apigenin-7-O-rutinoside,Ap7R	参考文献[29]

时期stage	Cy3R	Ap7G	Ap7R	Vicenin-2	Qu3R	Km7G	Km3G7R	Km3R	合计total
S1	0.47±0.01 a	1.51±0.01 a	1.99±0.01 b	0.43±0.01 d	2.04±0.04 a	1.68±0.01 a	0.69±0.04 b	0.85±0.14 a	9.19±0.04 a
S2	0.19±0.01 b	1.36±0.01 a	2.06±0.01 b	0.47±0.01 d	1.94±0.02 a	1.31±0.03 b	0.69±0.02 b	0.96±0.02 a	8.80±0.07 b
S3	—	0.99±0.01 c	2.05±0.03 b	0.62±0.01 c	1.78±0.01 b	1.41±0.02 b	0.95±0.02 b	0.73±0.02 a	8.53±0.07 c
S4	—	0.50±0.03 d	1.47±0.03 c	1.25±0.02 b	0.91±0.01 c	1.16±0.01 c	0.83±0.01 b	0.25±0.01 b	6.37±0.04 d
S5	—	0.61±0.04 d	2.91±0.05 a	1.84±0.03 a	0.67±0.01 c	1.09±0.01 c	1.25±0.02 a	0.27±0.01 b	8.64±0.07 c

色素成分 pigment composition	L^*	a^*	b^*	C^*
叶绿素 chlorophyll	-0.469	-0.704^**	-0.035	-0.603^*
类胡萝卜素 carotenoid	-0.519^*	-0.714^**	-0.206	-0.626^*
总类黄酮 total flavonoids	-0.102	0.608^*	0.181	0.429
Cy3R	-0.447	0.936^**	-0.306	0.475
Ap7G	0.009	0.903^**	-0.019	0.508
Ap7R	-0.219	-0.275	0.312	-0.200
Vicenin-2	-0.370	-0.786^**	0.018	-0.586^*
Qu3R	0.321	0.814^**	0.046	0.614^*
Km7G	0.016	0.852^**	-0.072	0.834^**
Km3G7R	-0.037	-0.785^**	0.333	-0.279
Km3R	0.291	0.782^**	0.077	0.464

彩叶桂叶片发育过程中叶色表型与色素成分变化

Changes of coloration and pigment compositions during leaf development of Osmanthus fragrans colour group cultivar

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