彩叶桂叶片发育过程中叶色表型与色素成分变化

崔祺; 吴昀; 李东泽; 吴凡; 韩蕊莲; 黄均华; 胡绍庆

doi:10.12302/j.issn.1000-2006.202109057

崔祺, 吴昀, 李东泽, 吴凡, 韩蕊莲, 黄均华, 胡绍庆

南京林业大学学报（自然科学版） ›› 2023, Vol. 47 ›› Issue (2) : 79-86.

PDF(1863 KB)

国家林草科技领军期刊
中国精品科技期刊
中国高校百佳科技期刊
江苏省新闻出版政府奖期刊奖
RCCSE林学权威期刊（A+）
CSCD核心期刊
Scopus数据库收录期刊
中文核心期刊
SCD核心期刊

作者加群：102861116

微信公众号：南京林业大学学报

高级检索

PDF(1863 KB)

南京林业大学学报（自然科学版） ›› 2023, Vol. 47 ›› Issue (2) : 79-86. DOI: 10.12302/j.issn.1000-2006.202109057

研究论文

彩叶桂叶片发育过程中叶色表型与色素成分变化

崔祺 ¹ ,
吴昀 ¹ ,
李东泽 ¹^,^* ,
吴凡 ¹ ,
韩蕊莲 ¹^,² ,
黄均华 ¹ ,
胡绍庆 ¹

作者信息 +

Changes of coloration and pigment compositions during leaf development of Osmanthus fragrans colour group cultivar

CUI Qi ¹ ,
WU Yun ¹ ,
LI Dongze ¹^,^* ,
WU Fan ¹ ,
HAN Ruilian ¹^,² ,
HUANG Junhua ¹ ,
HU Shaoqing ¹

Author information +

文章历史 +

摘要

【目的】探究彩叶桂叶片发育过程中叶色表型与色素成分的关系。【方法】以彩叶桂品种‘虔南桂妃’(Osmanthus fragrans ‘Qiannan Guifei’)5个发育时期(S1—S5)的叶片为研究对象,选用英国皇家园艺学会比色卡和色差仪测量其叶色表型,同时采用可见光分光光度法对5个时期的叶片进行叶绿素和类胡萝卜素的测定,利用超高效液相色谱-串联三重四级杆-飞行时间质谱(UPLC-Triple-TOF/MS)联用技术定性定量分析叶片中类黄酮成分与含量,进一步分析了叶色与各色素成分之间的相关性。【结果】随着叶片不断发育,彩叶桂叶片颜色变化大致分为两个阶段:第1个阶段由红转黄(S1—S3),第2个阶段由黄转绿(S3—S5)。叶片颜色参数明度L^*值和蓝度b^*值先增加后下降,而红a^*值和彩度C^*值则呈下降趋势。在叶片中共鉴定出了8种类黄酮,包括1种花青素(Cy3R)、3种黄酮(Ap7G、Ap7R和vicenin-2)以及4种黄酮醇(Km3R、Km7G、Km3G7R、Qu3R)。定量分析发现:叶绿素和类胡萝卜素在S1—S3时期含量较低且较为稳定,在S3—S5时期二者含量及其比值呈显著增加的趋势;Cy3R的含量在S1—S3时期显著降低,在S3—S5时期含量极低,未检测出;Qu3R、Km3R、Km7G和Ap7G含量从S1—S5显著降低,而vicenin-2和Km3G7R含量则呈现出持续增加的趋势;Ap7R的含量在S1—S3时期较为稳定,之后先下降后增加。【结论】彩叶桂‘虔南桂妃’叶色由红转黄主要是因为花青素(Cy3R)、黄酮(Ap7G)和黄酮醇(Qu3R、Km3R和Km7G)含量的降低,由黄转绿则是因为叶绿素和类胡萝卜素含量的比值增加。

Abstract

【Objective】 This study aimed to explore the relationship between leaf color changes and pigment composition in Osmanthus fragrans color group during the leaf development. 【Method】 Different-colored leaves of the O. fragrans color group cultivar ‘Qiannan Guifei’ were selected from five developmental stages (S1-S5) for phenotype mensuration using the Royal Horticultural Society Color Chart and colorimeter. Chlorophyll and carotenoid concentrations of the leaves were determined using visible light spectrophotometry. Flavonoids were qualitatively and quantitatively characterized using ultra-performance liquid chromatography triple-time-of-flight/mass spectrometry (UPLC-Triple-TOF/MS). The correlation between leaf color and pigment components was also analyzed. 【Result】 The results indicated that the changes in leaf color could be roughly divided into two stages in relation to the leaf development: the first stage was from red to yellow (S1-S3), and the second stage was from yellow to green (S3-S5). The color parameters L^* and b^* initially increased and then decreased, while the values of a^* and C^* decreased. Eight flavonoids were detected in the leaves of ‘Qiannan Guifei’, including one anthocyanidin (Cy3R), three flavones (Ap7G, Ap7R and vicenin-2), and four flavonols (Km3R, Km7G, Km3G7R and Qu3R). The quantitative analysis showed that chlorophyll and carotenoid concentrations were relatively low and stable during stages S1-S3, whereas the concentrations and ratios of the two pigments increased significantly during stages S3-S5. Cy3R concentration significantly decreased from stages S1 to stage S3, and it occurred in undetectable trace amounts in stages S3-S5. The concentrations of Qu3R, Km3R, Km7G and Ap7G also decreased from stage S1 to stage S5, while vicenin-2 and Km3G7R showed a continuous increase. Ap7R was stable from stage S1 to stage S3, and then it initially decreased and then increased. 【Conclusion】 The decrease of anthocyanidin (Cy3R), flavone (Ap7G) and flavonols (Qu3R, Km3R and Km7G) was critical in promoting changes in leaf color from red to yellow in ‘Qiannan Guifei’, while the increase in the ratio of chlorophylls to carotenoids resulted in the leaves changing from yellow to green.

导出引用

崔祺, 吴昀, 李东泽, 等. 彩叶桂叶片发育过程中叶色表型与色素成分变化[J]. 南京林业大学学报（自然科学版）. 2023, 47(2): 79-86 https://doi.org/10.12302/j.issn.1000-2006.202109057

CUI Qi, WU Yun, LI Dongze, et al. Changes of coloration and pigment compositions during leaf development of Osmanthus fragrans colour group cultivar[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2023, 47(2): 79-86 https://doi.org/10.12302/j.issn.1000-2006.202109057

中图分类号： S685.13

参考文献

列表( 原文顺序 | 文献年度倒序 | 文中引用次数倒序 ) 可视化分析

[1]	向其柏, 刘玉莲. 中国桂花品种图志[M]. 杭州: 浙江科学技术出版社, 2008. XIANG Q B, LIU Y L. An illustrated monograph of the sweet osmanthus cultivars in China[M]. Hangzhou: Zhejiang Science & Technology Press, 2008. 本文引用 [1]

[2]	向民, 段一凡, 向其柏. 木犀属品种国际登录中心年报(1)彩叶桂品种群的建立[J]. 南京林业大学学报(自然科学版), 2014, 38(1):2,187. XIANG M, DUAN Y F, XIANG Q B. Establishment of a new group-Osmanthus fragrans Colour Group[J]. J Nanjing For Univ (Nat Sci Ed), 2014, 38(1):2,187. 本文引用 [1]

[3]	冯园园, 李清莹, 胡绍庆. 彩叶桂品种资源与研究现状[J]. 浙江理工大学学报(自然科学版), 2019, 41(6):793-798. FENG Y Y, LI Q Y, HU S Q. Resources and research status of Osmanthus fragrans Colour Group cultivars[J]. J Zhejiang Sci Tech Univ (Nat Sci Ed), 2019, 41(6):793-798. 本文引用 [1]

[4]

冯园园, 李清莹, 黄均华, 等. 25个彩叶桂无性系(品种)的数量分类研究[J]. 南京林业大学学报(自然科学版), 2021, 45(1):107-115.

FENG

Y Y

, LI

Q Y

, HUANG

J H

, et al. Numerical classification of 25 color-leafed Osmanthus fragrans clones (cultivars)[J]. J Nanjing For Univ (Nat Sci Ed), 2021, 45(1):107-115.DOI:10.12302/j.issn.1000-2006.201911045.

本文引用 [1]

[5]	商彩丽, 刘青, 王明晓, 等. 植物叶色变异的分子机理研究进展[J]. 山东农业科学, 2021, 53(7):127-134. SHANG C L, LIU Q, WANG M X, et al. Research progress on molecular mechanisms of leaf color variation in plants[J]. Shandong Agric Sci, 2021, 53(7):127-134.DOI:10.14083/j.issn.1001-4942.2021.07.021. 本文引用 [1]

[6]	张磊, 曹德美, 胡建军. 植物叶色形成调控机制研究进展[J]. 植物遗传资源学报, 2021, 22(2):293-303. ZHANG L, CAO D M, HU J J. Advance of the regulation mechanism of leaf color formation in plants[J]. J Plant Genet Resour, 2021, 22(2):293-303.DOI:10.13430/j.cnki.jpgr.20200805001. 本文引用 [1]

[7]

朱书香, 杨建民, 王中华, 等. 4种李属彩叶植物色素含量与叶色参数的关系[J]. 西北植物学报, 2009, 29(8):1663-1669.

ZHU

S X

, YANG

J M

, WANG

Z H

, et al. Relationship between pigment contents and leafcolor parameters of four leaf-colored species of Prunus[J]. Acta Bot Boreali Occidentalia Sin, 2009, 29(8):1663-1669.DOI:10.3321/j.issn:1000-4025.2009.08.026.

本文引用 [1]

[8]

冯露, 吴际洋, 鞠易倩, 等. 紫叶紫薇呈色生理及光合特性研究[J]. 北京林业大学学报, 2017, 39(12):93-101.

FENG

, WU

J Y

, JU

Y Q

, et al. Leaf color characteristics and photosynthetic characteristics of purple-leafed Lagerstroemia indica ‘Ebony Ember’[J]. J Beijing For Univ, 2017, 39(12):93-101.DOI:10.13332/j.1000-1522.20170238.

本文引用 [1]

[9]	YANG T, LI K T, HAO S X, et al. The use of RNA sequencing and correlation network analysis to study potential regulators of crabapple leaf color transformation[J]. Plant Cell Physiol, 2018, 59(5):1027-1042.DOI:10.1093/pcp/pcy044. 本文引用 [1]

[10]	江军, 谭志明, 王海涛, 等. 彩叶桂花新品种‘虔南桂妃’[J]. 园艺学报, 2015, 42(6):1219-1220. JIANG J, TAN Z M, WANG H T, et al. A new colorful sweet Osmanthus cultivar ‘Qiannan Guifei’[J]. Acta Hortic Sin, 2015, 42(6):1219-1220.DOI:10.16420/j.issn.0513-353x.2014-0517. 本文引用 [1]

[11]	马立辉, 杨琼, 谢英赞, 等. 3个彩叶桂花品种的扦插繁殖试验[J]. 林业调查规划, 2015, 40(5):153-156. MA L H, YANG Q, XIE Y Z, et al. Cutting propagation experiment of three colorful sweet-scented Osmanthus[J]. For Invent Plan, 2015, 40(5):153-156.DOI:10.3969/j.issn.1671-3168.2015.05.033. 本文引用 [1]

[12]	杨国栋, 陈林, 程岩. 彩叶桂品种 “虔南桂妃” 嫩枝扦插技术研究[J]. 林业调查规划, 2018, 43(2):42-46,53. YANG G D, CHEN L, CHENG Y. Study on softwood cutting techniques of Osmanthus fragrans “QiannanGuifei”[J]. For Invent Plan, 2018, 43(2):42-46,53.DOI:10.3969/j.issn.1671-3168.2018.02.008. 本文引用 [1]

[13]	禹霖, 柏文富, 李建挥, 等. ‘彩虹’彩桂扦插繁殖技术研究[J]. 林业科技, 2019, 44(1):35-38. YU L, BAI W F, LI J H, et al. Research on the propagation of Osmanthus fragrans Siji Group ‘Caihong’ cuttings[J]. For Sci Technol, 2019, 44(1):35-38.DOI:10.19750/j.cnki.1001-9499.2019.01.011. 本文引用 [1]

[14]	陈碧露, 张远福, 罗永松, 等. 两种彩叶桂花新品种叶绿素和花青素的比较分析[J]. 农业科学, 2018(9):1040-1044. CHEN B L, ZHANG Y F, LUO Y S, et al. Comparative analysis of chlorophyll and anthocyanin in two new colorful sweet Osmanthus cultivars[J]. Hans J Agric Sci, 2018(9):1040-1044. 本文引用 [1]

[15]

CHEN

, YANG

X L

, XIE

, et al. Biochemical and comparative transcriptome analyses reveal key genes involved in major metabolic regulation related to colored leaf formation in Osmanthus fragrans ‘Yinbi Shuanghui’ during development[J]. Biomolecules, 2020, 10: 549. DOI:10.3390/biom10040549

本文引用 [1]

[16]	LUO J R, SHI Q Q, NIU L X, et al. Transcriptomic analysis of leaf in tree peony reveals differentially expressed pigments genes[J]. Molecules, 2017, 22(2):324.DOI:10.3390/molecules22020324. 本文引用 [2]

[17]	HASHIMOTO F, TANAKA M, MAEDA H, et al. Changes in flower coloration and sepal anthocyanins of cyanic Delphinium cultivars during flowering[J]. Biosci.Biotechnol.Biochem., 2002, 66(8):1652-1659.DOI:10.1271/bbb.66.1652. 本文引用 [1]

[18]	WANG L S, SHIRAISHI A, HASHIMOTO F, et al. Analysis of petal anthocyanins to investigate flower coloration of Zhongyuan (Chinese) and Daikon Island (Japanese) tree peony cultivars[J]. J Plant Res, 2001, 114(1):33-43.DOI:10.1007/PL00013966. 本文引用 [1]

[19]

钟培星, 王亮生, 李珊珊, 等. 芍药开花过程中花色和色素的变化[J]. 园艺学报, 2012, 39(11):2271-2282.

ZHONG

P X

, WANG

L S

, LI

S S

, et al. The changes of floral color and pigments composition during the flowering period in Paeonia lactiflora Pallas[J]. Acta Hortic Sin, 2012, 39(11):2271-2282.DOI:10.16420/j.issn.0513-353x.2012.11.023.

本文引用 [1]

[20]	王宁杭. 望春玉兰花瓣类黄酮组成及转录组分析[D]. 杭州: 浙江农林大学, 2019. WANG N H. The flavonoid components and transcriptome analysis of Magnolia biondii[D]. Hangzhou: Zhejiang A & F University, 2019. 本文引用 [2]

[21]	SILVA D B, TURATTI I C C, GOUVEIA D R, et al. Mass spectrometry of flavonoid vicenin-2,based sunlight barriers in Lychnophora species[J]. Sci Rep, 2014, 4:4309.DOI:10.1038/srep04309. 本文引用 [2]

[22]	MENOZZI-SMARRITO C, WONG C C, MEINL W, et al. First chemical synthesis and in vitro characterization of the potential human metabolites 5-O-feruloylquinic acid 4'-sulfate and 4'-O-glucuronide[J]. J Agric Food Chem, 2011, 59(10):5671-5676.DOI:10.1021/jf200272m. 本文引用 [2]

[23]	BLAZICS B, ALBERTI Á, KURSINSZKI L, et al. Identification and LC-MS-MS determination of acteoside,the main antioxidant compound of Euphrasia rostkoviana,using the isolated target analyte as external standard[J]. J Chromatogr Sci, 2011, 49(3):203-208.DOI:10.1093/chrsci/49.3.203. 本文引用 [2]

[24]

李辛雷, 王佳童, 孙振元, 等. 金花茶和白色山茶及其3个杂交品种类黄酮成分与花色的关系[J]. 园艺学报, 2019, 46(6):1145-1154.

X L

, WANG

J T

, SUN

Z Y

, et al. Flavonoid components and their effects on flower colors in Camellia nitidissima,white C. Japonica and their three hybrid cultivars[J]. Acta Hortic Sin, 2019, 46(6):1145-1154.DOI:10.16420/j.issn.0513-353x.2018-0603.

本文引用 [2]

[25]	BERGANTIN C, MAIETTI A, CAVAZZINI A, et al. Bioaccessibility and HPLC-MS/MS chemical characterization of phenolic antioxidants in red chicory (Cichorium intybus)[J]. J Funct Foods, 2017, 33:94-102.DOI:10.1016/j.jff.2017.02.037. 本文引用 [2]

[26]	LUAN G X, WANG H L, LV H H, et al. Separation and purification of five flavone glucosides and one lignan from Caragana korshinskii Kom.by the combination of HSCCC and semi-preparative RPLC[J]. Chromatographia, 2016, 79(13/14):823-831.DOI:10.1007/s10337-016-3090-4. 本文引用 [2]

[27]

VIEIRA

G S

, MARQUES

A S F

, MACHADO

M T C

, et al. Determination of anthocyanins and non-anthocyanin polyphenols by ultra performance liquid chromatography/electrospray ionization mass spectrometry (UPLC/ESI-MS) in jussara (Euterpe edulis) extracts[J]. J Food Sci Technol, 2017, 54(7):2135-2144.DOI:10.1007/s13197-017-2653-1.

本文引用 [2]

[28]	NAIR V D, GOPI R, PANNEERSELVAM R. Isolation and structure elucidation of natural antioxidants from leaves of Rauvolfia beddomethe: an endemic/endangered medicinal plant from southwestern Ghats of India[J]. Pharm Chem J, 2012, 46(1):35-44.DOI:10.1007/s11094-012-0730-3. 本文引用 [2]

[29]	SEO O N, KIM G S, PARK S, et al. Determination of polyphenol components of Lonicera japonica Thunb.using liquid chromatography-tandem mass spectrometry:contribution to the overall antioxidant activity[J]. Food Chem, 2012, 134(1):572-577.DOI:10.1016/j.foodchem.2012.02.124. 本文引用 [2]

[30]	TANAKA Y, TSUDA S, KUSUMI T. Metabolic engineering to modify flower color[J]. Plant Cell Physiol, 1998, 39(11):1119-1126.DOI:10.1093/oxfordjournals.pcp.a029312. 本文引用 [1]

[31]	WANG Y G, FU J X, ZHANG C, et al. HPLC-DAD-ESI-MS analysis of flavonoids from leaves of different cultivars of sweet Osmanthus[J]. Molecules, 2016, 21(9):1224.DOI:10.3390/molecules21091224. 本文引用 [1]

[32]	孙明霞, 王宝增, 范海, 等. 叶片中的花色素苷及其对植物适应环境的意义[J]. 植物生理学通讯, 2003, 39(6):688-694. SUN M X, WANG B Z, FAN H, et al. Anthocyanins of leaves and their enviromental significance in plant stress responses[J]. Plant Physiol Commun, 2003, 39(6):688-694.DOI:10.13592/j.cnki.ppj.2003.06.051. 本文引用 [2]

[33]

葛雨萱, 周肖红, 刘洋, 等. 黄栌属种质资源、栽培繁殖、化学成分、叶色调控研究进展[J]. 园艺学报, 2014, 41(9):1833-1845.

Y X

, ZHOU

X H

, LIU

, et al. Recent advances in germplasm,cultivation,propagation,chemical components and leaf color regulation of Cotinus[J]. Acta Hortic Sin, 2014, 41(9):1833-1845.DOI:10.16420/j.issn.0513-353x.2014.09.015.

本文引用 [1]

[34]	SHEN J, JIANG C Q, YAN Y F, et al. Effect of increased UV-B radiation on carotenoid accumulation and total antioxidant capacity in tobacco (Nicotiana tabacum L.) leaves[J]. Genet Mol Res, 2017, 16(1): 2017Mar8;16(1).DOI:10.4238/gmr16018438. 本文引用 [1]

[35]	SHEN J Z, ZOU Z W, ZHANG X Z, et al. Metabolic analyses reveal different mechanisms of leaf color change in two purple-leaf tea plant (Camellia sinensis L.) cultivars[J]. Hortic Res, 2018, 5:7.DOI:10.1038/s41438-017-0010-1. 本文引用 [1]