冷藏条件下土样保存时间对甲螨(甲螨亚目)和跳虫(弹尾纲)类物种分离的影响

杜志琦; 孟庆繁; 冯立超; 葛佳荣; 黄劲斌; 史晶晶; 李洪锐; 岑祖才

doi:10.12302/j.issn.1000-2006.202112042

杜志琦, 孟庆繁, 冯立超, 葛佳荣, 黄劲斌, 史晶晶, 李洪锐, 岑祖才

南京林业大学学报（自然科学版） ›› 2023, Vol. 47 ›› Issue (2) : 213-218.

PDF(1397 KB)

国家林草科技领军期刊
中国精品科技期刊
中国高校百佳科技期刊
江苏省新闻出版政府奖期刊奖
RCCSE林学权威期刊（A+）
CSCD核心期刊
Scopus数据库收录期刊
中文核心期刊
SCD核心期刊

作者加群：102861116

微信公众号：南京林业大学学报

高级检索

PDF(1397 KB)

南京林业大学学报（自然科学版） ›› 2023, Vol. 47 ›› Issue (2) : 213-218. DOI: 10.12302/j.issn.1000-2006.202112042

研究论文

冷藏条件下土样保存时间对甲螨(甲螨亚目)和跳虫(弹尾纲)类物种分离的影响

杜志琦 ¹ ,
孟庆繁 ¹^,^* ,
冯立超 ²^,^* ,
葛佳荣 ¹ ,
黄劲斌 ¹ ,
史晶晶 ¹ ,
李洪锐 ¹ ,
岑祖才 ¹

作者信息 +

Effects of soil different preservation time on the soil animals separation results of Oribatida and Collembola by low temperature storage

DU Zhiqi ¹ ,
MENG Qingfan ¹^,^* ,
FENG Lichao ²^,^* ,
GE Jiarong ¹ ,
HUANG Jinbin ¹ ,
SHI Jingjing ¹ ,
LI Hongrui ¹ ,
CEN Zucai ¹

Author information +

文章历史 +

摘要

【目的】探究土壤在低温储存条件下,不同储存时间对土壤动物分离效果的影响,为土壤动物的大量采集与分离提供参考。【方法】于2018年10月6日在吉林市松花湖自然保护区天然蒙古栎(Quercus mongolica)林,采用5点采样法钻取不同深度的土壤,置于4 ℃冰箱内储存,分别于采集当日、7、14、21、28天,对土样内动物样品(甲螨亚目(Oribatida)与弹尾纲(Collembola)类物种,下文统称为甲螨与跳虫)采用Berlese-Tullgren分离法分离,在显微镜下对分离得到的甲螨和跳虫根据形态学特征进行种属鉴定。【结果】随土样保存时间的延长,甲螨、跳虫的个体数和物种数总体呈下降趋势;相同保存时间、不同土壤深度下,甲螨与跳虫的种类差异显著(P<0.05),总体呈下降趋势;在枯落物层收集到的甲螨与跳虫,其物种数量与5、10、15 cm土层下的物种数量差异显著,也呈现下降趋势。【结论】冷藏条件下土壤保存时间延长会降低中型土壤动物的分离效率,甲螨在土壤采集当天分离效果最好,而跳虫分离基本不受土壤冷藏保存时间的影响;甲螨和跳虫的收集主要在枯落物层,土壤深度增加,土壤动物减少。

Abstract

【Objective】 This research aims to explore the effects of different storage time on the separation effects of soil animals under the condition of soil low temperature storage, so as to provide a reference for a large number of collection and separation of soil animals.【Method】 On October 6, 2018, the natural Mongolian oak (Quercus mongolica) forest in Songhua Lake Nature Reserve, Jilin City, the soil at different depths was drilled by the five point sampling method, brought back to the laboratory and stored in the indoor 4 ℃ refrigerator. The Berlese-Tullgren method was used to separate the soil animal samples of Oribatida and Collembola on the same day, 7, 14, 21 and 28 days later. The species and genera of Oribatida and Collembola were determined according to the morphological characteristics under the microscope.【Result】 With the increase of preservation time, the individual number and the number of species of soil animals of Oribatida and Collembola showed a downward trend; Under the same preservation time and different depths, the species of Oribatida and Collembola had significant differences with other depths (P<0.05), and showed a downward trend. The number of species of Oribatida and Collembolan collected in litter layer was significantly different from that in 5, 10 and 15 cm soil layers (P<0.05), and also showed a downward trend.【Conclusion】 Increasing the soil preservation time under cold storage will reduce the separation efficiency of medium-sized soil animals. The separation effect of Oribatida is the best on the day of soil collection, and the isolation of Collembola was not affected by soil storage time. Oribatida and Collembola animals were mainly collected in the litter layer. The soil depth increased while the soil animals decreased.

导出引用

杜志琦, 孟庆繁, 冯立超, 等. 冷藏条件下土样保存时间对甲螨(甲螨亚目)和跳虫(弹尾纲)类物种分离的影响[J]. 南京林业大学学报（自然科学版）. 2023, 47(2): 213-218 https://doi.org/10.12302/j.issn.1000-2006.202112042

DU Zhiqi, MENG Qingfan, FENG Lichao, et al. Effects of soil different preservation time on the soil animals separation results of Oribatida and Collembola by low temperature storage[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2023, 47(2): 213-218 https://doi.org/10.12302/j.issn.1000-2006.202112042

中图分类号： S154.5;S714.3

参考文献

列表( 原文顺序 | 文献年度倒序 | 文中引用次数倒序 ) 可视化分析

[1]	GILLER P S. The diversity of soil communities, the ‘Poor man’s tropical rainforest’[J]. Biodiversity & Conservation, 1996, 5(2): 135-168. DOI:10.1007/BF00055827. 本文引用 [1]

[2]	BEST M L, WELSH J. The trophic role of a forest salamander: impacts on invertebrates, leaf litter retention, and the humification process[J]. Ecosphere, 2014, 5(2): 1-19. DOI:10.1890/ES13-00302.1. 本文引用 [1]

[3]	VARGO D L. Soil invertebrates of American Samoa[J]. Micronesica, 2000, 33: 1-10. 本文引用 [1]

[4]	贺纪正, 陆雅海, 傅伯杰. 土壤生物学前沿[M]. 北京: 科学出版社, 2015. HE J Z, LU Y H, FU B J. Frontier of soil biology[M]. Beijing: Science Press, 2015. 本文引用 [1]

[5]	尹文英. 土壤动物学研究的回顾与展望[J]. 生物学通报, 2001, 36(8): 1-3. YIN W Y. Review and prospect of soil zoology research[J]. Bulletin of Biology, 2001, 36(8): 1-3. DOI:10.3969/j.issn.0006-3193.2001.08.001 本文引用 [1]

[6]	黄红英, 李栋. 干漏斗实验装置的改进与创新[J]. 生物学通报, 2018, 53(1): 57-58. HUANG H Y, LI D. The improvement and innovation of Tullgren[J]. Bull Biol, 2018, 53(1): 57-58. DOI:10.3969/j.issn.0006-3193.2018.01.019. 本文引用 [1]

[7]	IRMLER U. Changes in the fauna and its contribution to mass loss and N release during leaf litter decomposition in two deciduous forests[J]. Pedobiologia, 2000, 44(2): 105-118. DOI:10.1078/S0031-4056(04)70032-3. 本文引用 [1]

[8]	殷秀琴, 马祝阳. Tullgren法对土壤动物的分离效率[J]. 东北师大学报(自然科学版), 2002, 34(2): 84-91. YIN X Q, MA Z Y. Segregate efficiency of Tullgren method to middle-small-sized soil animal[J]. J Northeast Norm Univ (Nat Sci Ed), 2002, 34(2): 84-91. DOI: 10.3321/j.issn:1000-1832.2002.02.017. 本文引用 [1]

[9]	魏雪, 吴鹏飞. 土壤松紧度对Tullgren法分离小型节肢动物的影响[J]. 西南农业学报, 2014, 27(3): 1136-1142. WEI X, WU P F. Influence of soil compactness on extraction of soil microarthropods by tullgren method[J]. Southwest China J Agric Sci, 2014, 27(3):1136-1142. DOI:10.16213/j.cnki.scjas.2014.03.015. 本文引用 [1]

[10]

铁烈华, 张林成, 冯茂松, 等. 烘虫温度和时间对中小型土壤动物烘虫分离效果的影响[J]. 四川农业大学学报, 2015, 33(1): 45-50.

TIE

L H

, ZHANG

L C

, FENG

M S

, et al. Baking temperature and time on the effect of soil meso-and micro-fauna separation results[J]. J Sichuan Agric Univ, 2015, 33(1): 45-50. DOI:10.16036/j.issn.1000-2650.2015.01.008.

本文引用 [1]

[11]	CARTER M, GREGORICH E. Soil sampling and methods of analysis[M].2nd Ed. Roca Rat: Lewis Publishing House, 2007. 本文引用 [1]

[12]	ANDRIUZZI W S, ADAMS B J, BARRETT J E, et al. Observed trends of soil fauna in the Antarctic dry valleys: early signs of shifts predicted under climate change[J]. Ecology, 2018, 99(2):312-321.DOI:10.1002/ecy.2090. 本文引用 [1]

[13]	尹文英. 中国亚热带土壤动物[M]. 北京: 科学出版社, 1992. YIN W Y. Subtropical soil animals of China[M]. Beijing: Science Press, 1992. 本文引用 [1]

[14]	李春鸣. 土壤样品的采集和处理[J]. 西北民族大学学报(自然科学版), 2003, 24(3): 74-75. LI C M. Collection and treatment of soil samples[J]. J Northwest Minor Univ Natl (Nat Sci), 2003, 24(3): 74-75. DOI:10.14084/j.cnki.cn62-1188/n.2003.03.020. 本文引用 [1]

[15]	吾玛尔·阿布力孜. 土壤螨类的采集与玻片标本的制作[J]. 生物学通报, 2012, 47(1): 57-59. Omar·Abliz. Collection of soil mites and preparation of slide specimens[J]. Microbiol China, 2012, 47(1): 57-59. DOI:10.3969/j.issn.0006-3193.2012.01.022. 本文引用 [1]

[16]	尹文英. 中国土壤动物检索图鉴[M]. 北京: 科学出版社, 1998. YIN W Y. Pictorical keys to soil animals of China[M]. Beijing: Science Press, 1998. 本文引用 [1]

[17]	青木淳一. 日本产土壤动物检索[M]. 东京: 东海大学出版会, 1993. 本文引用 [1]

[18]

李东育, 陈晓露, 韩大勇, 等. 不同管理模式下苹果园昆虫群落物种组成及其多样性[J]. 江苏农业学报, 2021, 37(3):589-596.

D Y

, CHEN

X L

, HAN

D Y

, et al. Species composition and diversity of insect community in apple orchards with different management modes[J]. Jiangsu J Agr Sci, 2021, 37(3):589-596. DOI:10.3969/j.issn.1000-4440.2021.03.006.

本文引用 [1]

[19]	勾影波, 苏永春. 土壤温度和含水量对螨类和弹尾类动物数量的影响[J]. 常熟理工学院学报, 2007(2): 57-62. GOU Y B, SU Y C. Effects of temperature and water content in soil on the quantity of Acarina and Collembola[J]. J Chang Inst Technol, 2007, 21(2): 57-62. DOI:10.16101/j.cnki.cn32-1749/z.2007.02.013. 本文引用 [1]

[20]	邱军, 傅荣恕. 土壤温湿度对甲螨和跳虫数量的影响[J]. 山东师大学报(自然科学版), 2004, 19(4): 72-74. QIU J, FU R S. The effects of temperature and soil water content for the number of oribatida and Collembola[J]. J Shandong Norm Univ (Nat Sci), 2004, 19(4): 72-74. DOI:10.3969/j.issn.1001-4748.2004.04.021. 本文引用 [1]

[21]	马辰. 长白山地森林土壤跳虫的分布格局及其对环境变化的响应[D]. 长春: 东北师范大学, 2019. MA C. The responses of soil Collembolan’s distribution pattern to environmental changes in the forests of Changbai Mountains[D]. Changchun: Northeast Normal University, 2019. 本文引用 [1]

[22]

牙克甫·艾散江, 吾玛尔·阿布力孜, 买尔旦·艾斯卡尔, 等. 新疆7种微生境的土壤甲螨多样性特征[J]. 浙江农业学报, 2020, 32(4): 696-704.

Yakup·Hasnjan, Omar·Abliz, Mardan·Askra, et al. Diversity characteristics of soil Oribatid mites in 7 microenvironments in Xinjiang[J]. Acta Agric Zhejiangensis, 2020, 32(4): 696-704. DOI:10.3969/j.issn.1004-1524.2020.04.17

本文引用 [1]

[23]	NIELSEN U N, OSLER G H R, VAN DER WAL R, et al. Soil pore volume and the abundance of soil mites in two contrasting habitats[J]. Soil Biol Biochem, 2008, 40(6): 1538-1541. DOI:10.1016/j.soilbio.2007.12.029. 本文引用 [1]