吴茱萸枝屑代料栽培榆黄蘑的潜力评价

叶建强; 张芳芳; 陈丽新; 黄卓忠; 蓝桃菊

doi:10.12302/j.issn.1000-2006.202203016

吴茱萸枝屑代料栽培榆黄蘑的潜力评价

叶建强, 张芳芳, 陈丽新, 黄卓忠, 蓝桃菊

南京林业大学学报（自然科学版） ›› 2024, Vol. 48 ›› Issue (2) : 166-174.

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南京林业大学学报（自然科学版） ›› 2024, Vol. 48 ›› Issue (2) : 166-174. DOI: 10.12302/j.issn.1000-2006.202203016

研究论文

吴茱萸枝屑代料栽培榆黄蘑的潜力评价

叶建强 ¹ ,
张芳芳 ¹^,² ,
陈丽新 ¹ ,
黄卓忠 ¹ ,
蓝桃菊 ¹^,^*

作者信息 +

Potentiality evaluation of Pleurotus citrinopileatus cultivation with Tetradium ruticarpum branch sawdust

Author information +

文章历史 +

摘要

【目的】开展吴茱萸(Tetradium ruticarpum)枝屑栽培食用菌的潜力评价,对延伸吴茱萸产业链和加快吴茱萸枝条降解具有重要意义,也为吴茱萸枝条的高效利用提供参考依据。【方法】以食用菌中栽培周期较短的榆黄蘑(Pleurotus citrinopileatus)为试验菌株,通过测定和分析吴茱萸枝屑、杂木屑和棉籽壳的营养成分,以杂木屑配方(CK1)和棉籽壳配方(CK2)为对照,探讨以吴茱萸枝屑代替CK1中20%(处理1)、40%(处理2)、60%(处理3)、80%(处理4)和100%(处理5)的杂木屑栽培榆黄蘑对其农艺性状、菌包基质降解和经济效益的影响,综合评价吴茱萸枝屑栽培榆黄蘑效果。【结果】吴茱萸枝屑的纤维素、半纤维素和木质素含量与杂木屑的无显著差异(P>0.05,下同),但其半纤维素和木质素含量分别比棉籽壳的低39.64%和16.55%;粗蛋白含量是杂木屑的4.25倍和棉籽壳的1.91倍,碳氮比是杂木屑的0.24倍和棉籽壳的0.55倍;在杂木屑中未检测到硒,而吴茱萸枝屑的硒含量是棉籽壳的7.69倍。吴茱萸枝屑代替杂木屑栽培榆黄蘑,虽然增加了投入,但对菌丝萌发时间、长势、采收时间影响小,且能加快采后恢复,显著增加产量、原料利用率和经济效益,在处理3、4、5时菌丝生长速率显著加快,菌包成活率达到100%,处理4和处理5时菌丝生长速率与CK2的无显著差异;生物学转化率在处理2、3、4、5时高于CK2,以处理4最好,但每包总鲜质量还是显著低于CK2;综合出菇率在处理3至处理4时高于CK1和CK2;菌包降解程度在处理4时最高,原料利用率为71.88%、纤维素降解率为78.08%、半纤维素降解率为86.38%、木质素降解率为71.18%;经济效益以处理4最好,显著好于CK1,相对CK2每包收益只少0.03元,且能显著降低投入、提高投入产出率。【结论】吴茱萸枝屑是一种比杂木屑和棉籽壳富含粗蛋白、硒的原材料,可代替纯杂木屑和纯棉籽壳栽培榆黄蘑,80%代替杂木屑的量可保证菌包成活率,还有较高的菌丝生长速率、产量、第4潮出菇率、高的原料降解率和经济收益,是一种很好的食用菌原料,具体配方为:吴茱萸枝屑62.4%(质量分数,下同)、杂木屑15.6%、麦麸20.0%、石灰1.0%、石膏1.0%。

Abstract

【Objective】 This study assesses the potential of using Tetradium ruticarpum branch sawdust for cultivating edible mushrooms. The research is significant for extending the T. ruticarpum industry chain and expediting branch degradation. It also offers a reference for efficient utilization of T. ruticarpum branches. 【Method】 The Pleurotus citrinopileatus, known for its short cultivation cycle, was chosen as the experimental strain. The nutritional content of T. ruticarpum branch sawdust, mixed wood sawdust and cottonseed hulls was analyzed. Tests involved replacing 20% (treatment 1), 40% (treatment 2), 60% (treatment 3), 80% (treatment 4) and 100% (treatment 5) of the mixed wood sawdust with T. ruticarpum branch sawdust. The effects on agronomic traits, fungus bag substrate degradation, and economic benefits in cultivating P. citrinopileatus were examined, comparing with the mixed wood sawdust (CK1) and cottonseed hulls (CK2) formulations. 【Result】 The cellulose, hemicellulose and lignin contents of T. ruticarpum sawdust were comparable to those of mixed wood sawdust (P>0.05), but its hemicellulose and lignin contents were 39.64% and 16.55% lower than those of cottonseed hulls. Its crude protein content was 4.25 times and 1.91 times that of mixed wood and cottonseed hulls, respectively. Its carbon-nitrogen ratio was 0.24 and 0.55 times that of the two. Selenium was not detected in mixed wood. Selenium content in T. ruticarpum sawdust was 7.69 times that of cottonseed hulls. Using T. ruticarpum sawdust increased inputs but had minimal impact on mycelium germination, growth and harvest times. It accelerated post-harvest recovery, significantly boosted yield, substrate utilization and economic benefits. Higher mycelial growth rates and fungus bag survival rates of 100% were observed in treatments 3,4 and 5, with no significant differences compared to CK2 in treatments 4 and 5. Biological efficiency was higher in treatments 2, 3, 4 and 5 than that of CK2, with treatment 4 yielding the best results. However, the total fresh weight was significantly lower than that of CK2. Fruiting rate higher than CK1 and CK2 was observed in treatments 3 and 4. Substrate degradation was the highest in treatment 4, with substrate utilization at 71.88%, cellulose degradation at 78.08%, hemicellulose degradation at 86.38%, and lignin degradation at 71.18%. Treatment 4 also showed the best economic performance, significantly better than CK1, and nearly equivalent to CK2, with only 0.03 yuan lower in profit per bag. It significantly reduced inputs and improved input-output ratio. 【Conclusion】 The T. ruticarpum branch sawdust is a material richer in crude protein and selenium than mixed wood sawdust and cottonseed hulls, and can replace pure mixed wood sawdust and pure cottonseed hulls for cultivating P. citrinopileatus. Replacing 80% of the mixed wood sawdust can guarantee a good survival rate of fungal packages and also a relatively high mycelial growth rate, yield, fourth flush yield rate, high material degradation and economic performance. It is a excellent edible fungi raw material, with a specific formulation of 62.4% (mass fraction, the same below) T. ruticarpum branch sawdust, 15.6% mixed wood sawdust, 20.0% wheat bran, 1.0% lime, and 1.0% gypsum.

导出引用

叶建强, 张芳芳, 陈丽新, 等. 吴茱萸枝屑代料栽培榆黄蘑的潜力评价[J]. 南京林业大学学报（自然科学版）. 2024, 48(2): 166-174 https://doi.org/10.12302/j.issn.1000-2006.202203016

YE Jianqiang, ZHANG Fangfang, CHEN Lixin, et al. Potentiality evaluation of Pleurotus citrinopileatus cultivation with Tetradium ruticarpum branch sawdust[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2024, 48(2): 166-174 https://doi.org/10.12302/j.issn.1000-2006.202203016

中图分类号： S464

参考文献

列表( 原文顺序 | 文献年度倒序 | 文中引用次数倒序 ) 可视化分析

[1]	WU Z, PETER H R, HONG D, et al. Flora of China[M]. Beijing:Science Press; St. Louis: Missouri Botanical Garden Press, 2008:66-70. 本文引用 [1]

[2]

王承南, 王红娟, 李平, 等. 不同产区三种吴茱萸有效成分的研究[J]. 中南林业科技大学学报, 2012, 32(8):86-89.

WANG

C N

, WANG

H J

, LI

, et al. Studies on effective components of three kinds of Evodia rutoecarpa in different producing areas[J]. J Central South Univ For Technol, 2012, 32(8):86-89.DOI: 10.3969/j.issn.1003-8981.2012.01.024.

本文引用 [1]

[3]	魏宝阳, 曹亮, 李顺祥, 等. 吴茱萸遗传多样性的AFLP分析[J]. 中国中药杂志, 2012, 37(3):278-282. WEI B Y, CAO L, LI S X, et al. AFLP analysis of genetic diversity of Evodia rutaecarpa[J]. China J Chin Mater Med, 2012, 37(3):278-282.DOI: 10.4268/cjcmm20120304 本文引用 [1]

[4]	刘珊珊, 尹元元, 闫利华, 等. 吴茱萸药用植物资源调查[J]. 中国中医药信息杂志, 2016, 23(9):5-9. LIU S S, YIN Y Y, YAN L H, et al. Investigation on resources of medicinal plant of euodiae fructus[J]. Chin J Inf Tradit Chin Med, 2016, 23(9):5-9.DOI: 10.3969/j.issn.1005-5304.2016.09.002. 本文引用 [1]

[5]	南宁新闻网. 邕宁区新乐村:吴茱萸飘香农民喜开颜[EB/OL]. http://www.nnnews.net/p/3007758.html,2019-07-25. Nanning News Network. Farmers were all smiles in the Evodia rutoecarpa harvest season of Xinle Village,Yongning[EB/OL]. http://www.nnnews.net/p/3007758.html,2019-07-25. 本文引用 [1]

[6]	广西壮族自治区农业科学院. 广西农业科学院科技扶贫典型案例汇编[M]. 北京: 中国农业出版社, 2020:5-7. Guangxi Academy of Agricultural Sciences. Compilation of typical cases of poverty alleviation through science and technology in Guangxi Academy of Agricultural Sciences[M]. Beijing: China Agriculture Press, 2020:5-7. 本文引用 [1]

[7]	敖礼林, 敖艳, 周元, 等. 吴茱萸丰产高效栽培关键技术[J]. 科学种养, 2020,(2):21-24. AO L L, AO Y, ZHOU Y, et al. The high yield and effect of Evodia rutoecarpa cultivation techniques[J]. Kuexue Zhongyang, 2020,(2): 21-24.DOI: 10.13270/j.cnki.kxzh.2020.02.009. 本文引用 [1]

[8]	杨会书, 汤龙华, 孙兴旭, 等. 木腐菌保育实验初探[J]. 中国食用菌, 2016, 35(5):73-76 YANG H S, TANG L H, SUN X X, et al. Preliminary study on conservation experiment of wood rot fungi[J]. Edible Fungi China, 2016, 35(5):73-76.DOI: 10.13629/j.cnki.53-1054.2016.05.018. 本文引用 [1]

[9]	陈永娟. 香菇代料栽培技术发展动向[J]. 浙江食用菌, 1996(2): 3-5. CHEN Y J. Development trend of Lentinula edodes cultivation technique on the raw material substitution[J]. Zhejiang Shiyongjun, 1996(2):3-5. 本文引用 [1]

[10]	蔡武纯. 木屑栽培香菇[J]. 食用菌, 1980(1):49. CAI W C. Using sawdust cultivated Lentinula edodes[J]. Edible Fungi, 1980(1):49. 本文引用 [1]

[11]

中国食用菌协会.中国食用菌协会关于印发全国食用菌2018年度产量、产值统计调查结果的函[OB/L]. https://mp.weixin.qq.com/s/ix9fCR9Wygz4rGfvAY7ypw,2020-03-19.

China Edible Fungi Association.A letter was printed and distributed concerning 2018 statistical survey results of yield and value of China edible fungi by China edible fungi association[OB/L]. https://mp.weixin.qq.com/s/ix9fCR9Wygz4rGfvAY7ypw,2020-03-19.

本文引用 [1]

[12]	黄卫华, 陈明杰, 陈洪雨, 等. 葡萄枝栽培金顶侧耳及其营养成分分析[J]. 食用菌学报, 2019, 26(4):64-71. HUANG W H, CHEN M J, CHEN H Y, et al. Pleurotus citrinopileatus cultivated on grape wood branches and its nutrient analysis[J]. Acta Edulis Fungi, 2019, 26(4): 64-71.DOI: 10.16488/j.cnki.1005-9873.2019.04.009. 本文引用 [2]

[13]	杨笑然. 热带作物基质对3种侧耳营养利用及产量品质的影响研究[D]. 长春: 吉林农业大学, 2019. YANG X L. Study on effects of tropical crop substrate for three Pleurotus spices on nutrition utilization,yield and quality[D]. Changchun: Jilin Agricultural University, 2019. 本文引用 [2]

[14]	张婧, 杜阿朋. 利用桉树木屑栽培榆黄蘑试验[J]. 食药用菌, 2017, 25(6):379-381. ZHANG J, DU A P. Cultivation experiment of Pleurotus citrinopileatus using Eucalyptus sawdust[J]. Edible and Medicinal Mushrooms, 2017, 25(6):379-381. 本文引用 [2]

[15]	MUSIEBA F, OKOTH S, MIBEY R K, et al. Suitability of locally available substrates for cultivation of the Kenyan indigenous golden oyster mushroom (Pleurotus citrinopileatus Singer)[J]. Am J Food Technol, 2012, 7(10):650-655.DOI: 10.3923/ajft.2012.650.655. 本文引用 [1]

[16]	郑安波, 郭莹. 玉米芯栽培榆黄蘑配方比较试验[J]. 食用菌, 2020, 42(6):37-38. ZHENG A B, GUO Y. Comparison experiment of the different corncob formulas on Pleurotus citrinopileatus's cultivation[J]. Edible Fungi, 2020, 42(6):37-38.DOI: 10.3969/j.issn.1000-8357.2020.06.012. 本文引用 [1]

[17]	何达崇, 李槐, 戴圣生, 等. 桑枝杆栽培优质榆黄蘑成分分析[J]. 食药用菌, 2013, 21(5):290-292. HE D C, LI H, DAI S A, et al. Analysis of Pleurotus citrinopileatus nutritional contents using nulberry branch sawdust cultivation[J]. Edible Med Mushrooms, 2013, 21(5):290-292. 本文引用 [1]

[18]

FREITAS

A C

, ANTUNES

M B

, RODRIGUES

, et al. Use of coffee by-products for the cultivation of Pleurotus citrinopileatus and Pleurotus salmoneo-stramineus and its impact on biological properties of extracts thereof[J]. Int J Food Sci Tech, 2018, 53(8):1914-1924.DOI: 10.1111/ijfs.13778.

本文引用 [1]

[19]	王雨琼, 周道玮. 白腐菌对玉米秸秆营养价值及抗氧化性能的影响[J]. 动物营养学报, 2017, 29(11)4108-4115 WANG Y Q, ZHOU D W. Effects of white rot fungi on nutritional value and antioxidant property of corn stover[J]. Chin J Animal Nutr, 2017, 29(11):4108-4115.DOI: 10.3969/j.issn.1006-267x.2017.11.033. 本文引用 [2]

[20]	石娇蕊. 降解木质素菌株筛选及玉米秸秆多菌种发酵条件优化[D]. 长春: 吉林大学, 2008. SHI J R. Screening microbial strains for lignin degradation and optimizing the fermentation condition of maize stover by multi-strains[D]. Changchun: Jilin University, 2008. 本文引用 [1]

[21]

中华人民共和国国家卫生和计划生育委员会, 国家食品药品监督管理总局.食品安全国家标准食品中蛋白质的测定:GB 5009.5-2016[S]. 北京: 中国标准出版社, 2016:1-3.

National Health and Family Planning Commission of the People's Republic of China,China Food and Drug Administration. Determination of protein in foods of National food safety standard[S]. Beijing: China Standard Press, 2016:1-3.

本文引用 [1]

[22]	熊素敏, 左秀凤, 朱永义. 稻壳中纤维素、半纤维素和木质素的测定[J]. 粮食与饲料工业, 2005(8): 40-41. XIONG S M, ZUO X F, ZHU Y Y. Determination of cellulose,hemicellulose and ligin in rice hull[J]. Cereal and Feed Industry, 2005(8): 40-41.DOI: 10.3969/j.issn.1003-6202.2005.08.018 本文引用 [1]

[23]	中华人民共和国农业农村部. 有机肥料:NY/T525-2021[S]. 北京: 中国农业出版社, 2021:7-9. Ministry of Agriculture and Rural Affairs of the People's Republic of China. Organic fertilizer[S]. Beijing: China Agricuture Press, 2021:7-9. 本文引用 [1]

[24]

中华人民共和国国家卫生和计划生育委员会, 国家食品药品监督管理总局.食品安全国家标准食品中硒的测定:GB 5009.93-2017[S]. 北京: 中国标准出版社, 2017:7.

National Health and Family Planning Commission of the People's Republic of China,China Food and Drug Administration. Determination of selenium in foods of national food safety standard[S]. Beijing: China Standard Press, 2017:7.

本文引用 [1]

[25]	中国食用菌协会.2019年度全国食用菌统计调查结果分析[OB/L]. https://mp.weixin.qq.com/s/uJgpEP5sG3-lf2ebgbWrcg,2020-12-22. China Edible Fungi Association.Analysis of 2019 statistical survey results of China edible fungi[OB/L]. https://mp.weixin.qq.com/s/uJgpEP5sG3-lf2ebgbWrcg,2020-12-22. 本文引用 [1]

[26]	梁连友. 渭北苹果枝条栽培袋料香菇技术研究与推广[D]. 杨凌: 西北农林科技大学, 2006. LIANG L Y. Study and extension of cultivating shii-take using branches of fruit tree as substrate in Weibei highland[D]. Yangling: Northwest A & F University, 2006. 本文引用 [3]

[27]	陈丽新, 黄卓忠, 韦仕岩. 葡萄枝营养成分分析及栽培秀珍菇试验[J]. 中国食用菌, 2010, 29(6):28-29. CHEN L X, HUANG Z Z, WEI S Y. Analysis of nutritional components of grape branches and cultivation of Pleurotus geesteranus[J]. Edible Fungi China, 2010, 29(6):28-29.DOI: 10.3969/j.issn.1003-8310.2010.06.009. 本文引用 [3]

[28]	王谦, 卢红, 王路遥. 荆条基质下毛木耳培养条件优化[J]. 北方园艺, 2017(12):146-150. WANG Q, LU H, WANG L Y. Culture conditions optimization for Auricularia polytricha in Vitex chip[J]. Northern Horticulture, 2017(12):146-150.DOI:10.11937/bfyy.201712033. 本文引用 [3]

[29]	孙波, 周洪英, 吴洪丽. 桑枝栽培食用菌研究进展[J]. 食用菌, 2015, 37(4):5-7. SUN B, ZHOU H Y, WU H L. Research progress on cultivation of edible fungi by mulberry branches[J]. Edible Fungi, 2015, 37(4):5-7.DOI: 10.3969/j.issn.1000-8357.2015.04.002. 本文引用 [1]

[30]	林金盛, 宋金俤, 陈余红. 利用桑树枝桠栽培香菇试验[J]. 食药用菌, 2014, 22(1): 53-54. LIN J S, SONG J D, CHEN Y. Cultivation experimental of Lentinula edodes by nulberry branch sawdust[J]. Edible and Medicinal Mushroom, 2014, 22(1): 53-54. 本文引用 [1]

[31]	李志刚, 陈霞娜, 丁李春, 等. 适宜葡萄枝屑栽培的香菇菌株筛选[J]. 食用菌, 2022, 44(1):21-23. LI Z G, CHEN X N, DING L C, et al. Screening of Lentinus edodes strains suitable for grape cuttings cultivation[J]. Edible Fungi, 2022, 44(1):21-23.DOI: 10.3969/j.issn.1000-8357.2022.01.006. 本文引用 [1]

[32]	李海铭, 张萌, 李兴, 等. 秸秆微生物降解的研究进展[J]. 山东化工, 2021, 50(9):70-72,74. LI H M, ZHANG M, LI X, et al. Research progress of straw degradation by microorganism[J]. Shandong Chem Ind, 2021, 50(9):70-72,74.DOI: 10.3969/j.issn.1008-021X.2021.09.030. 本文引用 [1]

[33]	刘林峰, 何建清, 张格杰, 等. 园林废弃物木质纤维素降解大型真菌的筛选及能力研究[J]. 高原农业, 2021(3):249-255. LIU L F, HE J Q, ZHANG G J, et al. Screening of degradation of large fungi for lignocellulose from garden waste and its capability[J]. J Plateau Agric, 2021, 5(3):249-255. 本文引用 [1]

[34]	吴桐, 贾锐鱼, 路强强, 等. 白腐菌在木质纤维素酶解中的研究进展[J]. 江苏农业科学, 2021, 49(5):38-45. WU T, JIA R Y, LU Q Q, et al. Research progress of white rot fungi in enzymatic hydrolysis of lignocellulose[J]. Jiangsu Agric Sci, 2021, 49(5):38-45.DOI: 10.15889/j.issn.1002-1302.2021.05.007. 本文引用 [1]

[35]	王彩荣, 李田春. 金顶侧耳降解基质能力的研究[J]. 辽宁农业科学, 1994(3): 27-28+16. WANG C R, LI T C. Study on substrate degradation capacity of Pleurotus citrinopileatus[J]. Liaoning Agricultural Sciences, 1994(3): 27-28,16. 本文引用 [1]

[36]	李楠. 吉林省黑木耳优质高效栽培技术的研究[D]. 长春: 吉林农业大学. LI N. Study on high-quality and efficient cultivation techniques of Auricularia auricula in Jilin Province[D]. Changchun: Jilin Agricultural University, 2008. 本文引用 [1]

[37]	张理堂, 肖兴云, 黄瑞贤, 等. 长白山区金顶侧耳栽培品种(系)比较试验[J]. 吉林蔬菜, 2018(4):43-44. ZHANG L T, XIAO X Y, HUANG R X, et al. Comparative experiment on cultivated varieties (lines) of Pleurotus citrinopileatus in Changbai Mountain area[J]. Jilin Veg, 2018(4):43-44.DOI: 10.16627/j.cnki.cn22-1215/s.2018.04.029. 本文引用 [1]

[38]

黄丹莲, 曾光明, 胡天觉, 等. 白腐菌应用于堆肥处理含木质素废物的研究[J]. 环境污染治理技术与设备, 2005, 6(2):29-32.

HUANG

D L

, ZENG

G M

, HU

T J

, et al. Preliminary study on the application of white-rot fungus in composting of lignin waste[J]. Tech Equip Environ Pollut Control, 2005, 6(2):29-32.DOI: 10.3969/j.issn.1673-9108.2005.02.007.

本文引用 [1]

[39]	HAN K J, MYOUNG-JUN J. Sawdust substitution in growth medium of oyster mushroom for using its by-product spent mushroom substrates as ruminant feed[J]. Korean J Mycol, 2020, 48:407-414.DOI: 10.4489/KJM.20200040. 本文引用 [1]

[40]	司徒成, 余天华, 宋宇欣. 不同食用菌对猕猴桃枝木质纤维素降解的比较[J]. 北方园艺, 2022(5):111-118. SITU C, YU T H, SONG Y X. Comparison on lignocellulose degradation of kiwifruit branches by different edible fungi[J]. North Hortic, 2022(5):111-118.DOI: 10.11937/bfyy.20213707. 本文引用 [1]

[41]

陈国龙, 秦延春, 卢玉文, 等. 不同培养料对秀珍菇生长发育及主要营养成分的影响[J]. 食用菌, 2018, 40(6):33-34,49.

CHEN

G L

, QIN

Y C

, LU

Y W

, et al. Effects of different culture materials on the growth and development of Pleurotus geesteranus and its main nutritional components[J]. Edible Fungi, 2018, 40(6):33-34,49.DOI: 10.3969/j.issn.1000-8357.2018.06.012.

本文引用 [1]

[42]

杨士锋, 张健, 刘军鹏, 等. 应用灰色关联度法筛选香菇栽培料中柠条屑添加量[J]. 食用菌学报, 2017, 24(2):10-14.

YANG

S F

, ZHANG

, LIU

J P

, et al. Optimization of Caragana korshinskii sawdust supplement in a Lentinula edodes cultivation substrate using grey correlation analysis[J]. Acta Edulis Fungi, 2017, 24(2):10-14.DOI: 10.16488/j.cnki.1005-9873.2017.02.03.

本文引用 [1]

[43]

中华人民共和国国务院. 国务院关于印发“十三五”生态环境保护规划的通知[J]. 中华人民共和国国务院公报, 2016(35):40-76.

The State Council of the People's Republic of China. Notice on the issuance of the national plan of the ecological environment protection for the 13th Five-Year Plan[J]. Gazette State Counc People's Repub China, 2016(35):40-76.

本文引用 [1]