不同楸树品种对茎腐病的抗性差异研究

doi:10.12302/j.issn.1000-2006.202203017

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南京林业大学学报（自然科学版） ›› 2024, Vol. 48 ›› Issue (2): 155-165.doi: 10.12302/j.issn.1000-2006.202203017

不同楸树品种对茎腐病的抗性差异研究

祝艳艳(), 贾瑞瑞, 付钰, 常林, 岳远征, 杨秀莲, 王良桂^*()

南京林业大学风景院林学院,江苏省风景园林重点实验室,江苏南京 210037

收稿日期:2022-03-06 修回日期:2022-04-19 出版日期:2024-03-30 发布日期:2024-04-08
通讯作者: *王良桂(wlg@njfu.com.cn),教授。
作者简介:祝艳艳(3151680171@qq.com)。
基金资助:
江苏省农业科技自主创新资金项目(CX(19)2038);江苏省林业科技创新与推广项目(LYKJ(20019)05)

Differences in resistance of Catalpa bungei cultivars to stem rot

ZHU Yanyan(), JIA Ruirui, FU Yu, CHANG Lin, YUE Yuanzheng, YANG Xiulian, WANG Lianggui^*()

College of Landscape Architecture, Nanjing Forestry University,Jiangsu Provincial Key Laboratory of Landscape Architecture,Nanjing 210037,China

Received:2022-03-06 Revised:2022-04-19 Online:2024-03-30 Published:2024-04-08

摘要/Abstract

摘要：

【目的】探究不同品种和不同繁殖方式楸树(Catalpa bungei)苗对烟草疫霉(Phytophthora nicotianae)(菌株QS.1)侵染的抗性差异,为楸树引种栽培、抗病育种提供依据。【方法】以‘洛楸1号’组培苗(LQ-Z)、‘金丝楸’(JSQ)、‘楸杂1号’(QZ)、‘豫楸1号’(YQ)的1年生组培苗,以及‘洛楸1号’扦插苗(LQ-Q)、‘楸树5-8’(Q5-8)、‘楸树8-1’(Q8-1)的1年生扦插苗,6个生产上推广的楸树品种为试验材料,用烟草疫霉(菌株QS.1)进行侵染,测定楸树相关渗透物质含量、保护酶活性和光合气体参数等变化,分析不同处理楸树幼苗感染烟草疫霉(菌株QS.1)后的生理指标变化。【结果】菌株QS.1侵染后2~4 d,供试的各楸树品种均感病。楸树品种组培苗中,‘楸杂1号’和‘豫楸1号’对菌株QS.1侵染的抗性最弱,‘金丝楸’的抗病力最强;楸树品种扦插苗中,‘楸树5-8’和‘楸树8-1’对菌株QS.1侵染抗性较强;‘洛楸1号’扦插苗抗病性大于组培苗,除‘金丝楸’外,楸树品种的扦插苗较组培苗具有更高的抗性。【结论】不同楸树品种在接菌后感染致病存在差异,不同楸树品种的抗烟草疫霉(菌株QS.1)致病能力与光合性能的维持及防御相关酶活性的调节有关。

关键词: 楸树品种, 烟草疫霉(菌株QS.1), 抗性差异, 生理指标, 繁殖方式

Abstract:

【Objective】 To investigate the differences in resistance to Phytophthora nicotianae (strain QS.1) infestation by Catalpa bungei cultivars and breeding methods, providing basis for C. bungei introduction and cultivation and disease resistance breeding. 【Method】 The annual seedlings of ‘Jinsiqiu’ (JSQ),‘Qiuza 1 hao’ (QZ),‘Yuqiu 1 hao’ (YQ),‘Luoqiu 1 hao’ (LQ-Z)tissue cultured seedlings,and cutting seedling‘Luoqiu 1 hao’(LQ-Q),‘Qiu5-8’(Q5-8), ‘Qiu 8-1’(Q8-1),six C. bungei cultivars were used as experimental materials and infested with P. nicotianae strain QS.1 to determine relevant osmotic substance content,protective enzyme activity and photosynthetic gas parameters. 【Result】 After infection 2-4 d by strain QS.1,all C. bungei cultivars tested were susceptible to the disease and in the case of seedlings ‘Qiuza 1 hao’ and ‘Yuqiu 1 hao’ had the weakest resistance to the disease caused by the strain QS.1,while ‘Jinsiqiu’ had the strongest resistance to the disease; as far as the cuttings of C. bungei cultivars were concerned,Q5-8 and Q8-1 were more resistant to the infection of strain QS.1; ‘Luoqiu 1 hao’ cuttings were more resistant to the disease than the histoculture seedlings,and the cuttings of other species had stronger resistance to QS.1 infection than the histoculture seedlings,except for‘JSQ’.【Conclusion】 There were differences in infection pathogenesis among different C. bungei cultivars after inoculation. The pathogenic ability of different C. bungei cultivars against P. nicotianae strain QS.1 is related to the maintenance of photosynthetic performance and the regulation of defense-related enzyme activities.

Key words: Catalpa bungei cultivars, Phtophthora nicotianae (strain QS.1), resistance difference, physiological index, propagation method

中图分类号:

S763.7

祝艳艳,贾瑞瑞,付钰,等. 不同楸树品种对茎腐病的抗性差异研究[J]. 南京林业大学学报（自然科学版）, 2024, 48(2): 155-165.

ZHU Yanyan, JIA Ruirui, FU Yu, CHANG Lin, YUE Yuanzheng, YANG Xiulian, WANG Lianggui. Differences in resistance of Catalpa bungei cultivars to stem rot[J].Journal of Nanjing Forestry University (Natural Science Edition), 2024, 48(2): 155-165.DOI: 10.12302/j.issn.1000-2006.202203017.

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繁殖方式 propagation methods	楸树品种 varieties	发病率/% incidence rate	感病指数/% disease infected index	抗病评价 resistance evaluation
组培 tissue culture	JSQ	16.67	8.58±0.54 c	高抗
	QZ	70.37	62.04±0.80 a	高感
	YQ	68.96	61.76±0.66 a	高感
	LQ-Z	60.00	44.49±0.50 b	中抗
扦插 cutting	LQ-Q	28.00	9.51±0.64 c	高抗
	Q5-8	55.00	41.36±0.74 a	中抗
	Q8-1	44.00	38.36±0.79 b	中抗

相关因子 correlation factors	CAT	SOD	POD	Pro	SS	SP	REC	Chla	Chlb	Ci	Gs	Pn	Tr
CAT	1
SOD	0.662^**	1
POD	0.711^**	0.647^**	1
Pro	-0.040	-0.324	0.002	1
SS	0.461^*	0.298	0.605^**	0.340	1
SP	0.555^**	0.403^*	0.381^*	0.107	0.280	1
REC	-0.267	-0.578^**	-0.252	0.104	-0.184	-0.050	1
Chla	0.398^*	0.487^**	0.253	-0.384^*	0.097	0.512^**	-0.122	1
Chlb	0.391^*	0.496^**	0.253	-0.329	0.175	0.549^**	-0.104	0.952^**	1
Ci	0.155	0.281	0.194	0.128	0.221	0.256	-0.351	-0.115	0.003	1
Gs	0.273	0.451^*	0.021	-0.194	-0.160	0.560^**	-0.427^*	0.572^**	0.585^**	0.160	1
Pn	0.227	0.365	-0.102	-0.200	-0.228	0.411^*	-0.358	0.643^**	0.606^**	-0.136	0.879^**	1
Tr	0.213	0.433^*	0.060	-0.315	-0.132	0.309	-0.377^*	0.540^**	0.549^**	0.016	0.755^**	0.735^**	1

相关因子 correlation factors	因子载荷load
相关因子 correlation factors	1	2	3	4
CAT	0.822	-0.149	0.382	0.013
SOD	0.817	-0.207	0.411	-0.064
POD	0.802	-0.373	-0.247	0.287
Pro	0.779	0.328	-0.196	-0.344
SS	0.745	-0.524	-0.086	0.212
SP	0.726	-0.403	-0.198	0.017
REC	0.673	0.221	0.221	0.493
Chla	0.643	0.539	0.148	-0.078
Chlb	0.203	0.797	0.161	0.102
Ci	0.457	0.745	0.133	-0.249
Gs	-0.475	-0.150	0.727	0.176
Pn	0.169	0.420	-0.575	0.228
Tr	-0.309	0.392	-0.041	0.753
特征值eigenvalue	5.142	2.630	1.436	1.221
贡献率/% contribution rate	39.556	30.229	11.048	9.392
累计贡献率/% cumulative contribution rate	39.556	69.785	80.833	90.225

品种 species	各因子得分 score of each factor				综合得分 compre- hensive score	排序 sorting
品种 species	1	2	3	4	综合得分 compre- hensive score	排序 sorting
JSQ	0.991	1.645	1.196	-0.269	0.831	2
QZ	-1.964	-0.894	0.907	0.338	-0.827	6
YQ	-2.654	-0.434	0.325	0.663	-1.041	7
LQ-Z	0.795	-1.330	-0.567	0.596	0.040	4
LQ-Q	2.683	-0.538	-0.603	0.443	0.929	1
Q5-8	-0.048	-0.111	-1.174	-0.152	-0.185	5
Q8-1	0.198	1.663	-0.085	-1.619	0.253	3

不同楸树品种对茎腐病的抗性差异研究

Differences in resistance of Catalpa bungei cultivars to stem rot

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