C源与NP添加对Cd胁迫下林地土壤呼吸作用的影响

doi:10.12302/j.issn.1000-2006.202204011

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南京林业大学学报（自然科学版） ›› 2024, Vol. 48 ›› Issue (1): 140-146.doi: 10.12302/j.issn.1000-2006.202204011

C源与NP添加对Cd胁迫下林地土壤呼吸作用的影响

孙劲伟¹(), 王圣燕¹, 范弟武¹, 朱咏莉¹^,²^,³^,^*()

1.南京林业大学生态与环境学院,江苏南京 210037
2.南京林业大学南方现代林业协同创新中心,江苏南京 210037
3.江苏洪泽湖湿地生态系统国家定位观测研究站,江苏洪泽 223100

收稿日期:2022-04-07 修回日期:2022-06-16 出版日期:2024-01-30 发布日期:2024-01-24
通讯作者: 朱咏莉
基金资助:
国家自然科学基金项目(41977354);江苏高校优势学科建设工程资助项目(PAPD)

Effects of C, N and P additions on soil respiration in woodland under Cd stress

SUN Jinwei¹(), WANG Shengyan¹, FAN Diwu¹, ZHU Yongli¹^,²^,³^,^*()

1. College of Ecology and Environment, Nanjing Forestry University, Nanjing 210037,China
2. Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China
3. National Positioning Observation Station of Hung-tse Lake Wetland Ecosystem in Jiangsu Province, Hongze 223100, China

Received:2022-04-07 Revised:2022-06-16 Online:2024-01-30 Published:2024-01-24
Contact: ZHU Yongli

摘要/Abstract

摘要：

【目的】研究C源与矿质营养氮磷(NP)添加对土壤呼吸作用毒物兴奋效应(Hormesis)的影响。【方法】以模式土壤为对象,接种杨树林地土壤微生物,设置单独添加葡萄糖(GC)、单独添加矿质营养NP(NP)、同时添加葡萄糖和NP(GC+NP),以及二者均无添加的对照(CK)4个处理,研究Cd胁迫下土壤呼吸作用潜在的Hormesis效应。【结果】①NP和GC+NP处理,林地土壤呼吸速率在Cd剂量为0.02、0.10、0.40、2.50、13.00 mg/kg时均显著高于对照,且表现出显著的多重Hormesis效应交替出现的现象,刺激幅度变化在66.6%~262.6%。②土壤中不添加Cd时,GC与NP处理土壤呼吸速率之和大于GC+NP处理,土壤呼吸对二者的添加表现为拮抗效应;当Cd剂量在0.01~0.20 mg/kg区间时,GC与NP处理土壤呼吸速率之和小于GC+NP处理,C源与NP添加对土壤呼吸的影响表现为协同效应;Cd剂量>0.20 mg/kg时,二者之间表现为协同与拮抗效应的交替出现。【结论】外源添加N、P条件下,Cd诱导林地土壤呼吸表现出显著的Hormesis效应。当面临逐渐增加的Cd胁迫时,C源与NP添加对土壤呼吸的交互作用表现为由拮抗向协同效应转变。

关键词: 土壤呼吸, Cd胁迫, 毒物兴奋效应, 模式土壤, 葡萄糖, 氮磷添加

Abstract:

【Objective】 Artificial standard soil was used to investigate the potential stimulatory effects of low-dose C, N, and P additions on soil respiration and Hormesis under heavy metal stress. 【Method】The four treatments were: GC (glucose), NP (nitrogen and phosphorus), GC+NP (glucose, nitrogen and phosphorus), and, no additions (CK). The soil samples were inoculated with soil microorganisms from forest land to determine the potential Hormesis effect of exogenous addition of glucose, N and P on soil respiration under Cd stress. 【Result】In the case of the NP and GC+NP treatments, the soil respiration rate was significantly higher than that of the control at Cd doses of 0.02, 0.10, 0.40, 2.50, and 13.00 mg/kg, respectively. There was a significant alternating phenomenon of multiple hormetic effects with stimulation amplitudes between 66.6% and 262.6%. When there was no Cd added to the soil, the sum of the soil respiration rates in the GC and NP treatments was greater than that in the GC+NP treatment. The interaction between C source and NP addition on soil respiration showed an antagonistic effect. When the Cd dose was 0.01 to 0.20 mg/kg, the sum of soil respiration rates in GC and NP treatments was lower than the corresponding rates in GC+NP treatments, and the effects of C source and NP additions on soil respiration showed a synergistic effect. Synergistic and antagonistic effects appeared alternately when the Cd dose was over 0.20 mg/kg. 【Conclusion】The Cd-induced soil respiration rate had a significant Hormesis effect under exogenous NP addition. With increasing Cd stress, the interaction between the C source and NP addition on soil respiration changed from antagonistic to synergistic effects.

Key words: soil respiration, cadmium stress, hormesis, artificial standard soil, glucose, nitrogen and phosphorus addition

中图分类号:

孙劲伟,王圣燕,范弟武,等. C源与NP添加对Cd胁迫下林地土壤呼吸作用的影响[J]. 南京林业大学学报（自然科学版）, 2024, 48(1): 140-146.

SUN Jinwei, WANG Shengyan, FAN Diwu, ZHU Yongli. Effects of C, N and P additions on soil respiration in woodland under Cd stress[J].Journal of Nanjing Forestry University (Natural Science Edition), 2024, 48(1): 140-146.DOI: 10.12302/j.issn.1000-2006.202204011.

图/表 3

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图2

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剂量/ (mg·kg^-1) dose	R/(mg·kg^-1·h^-1)			交互效应/% interaction	剂量/ (mg·kg^-1) dose	R/(mg·kg^-1·h^-1)			交互效应/% interaction
剂量/ (mg·kg^-1) dose	GC-CK	NP-CK	(GC+NP)-CK	交互效应/% interaction	剂量/ (mg·kg^-1) dose	GC-CK	NP-CK	(GC+NP)-CK	交互效应/% interaction
0(CK)	18.5±9.2	0.2±5.2	16.9±11.7	拮抗	0.60	10.9±6.6	-2.6±1.5	10.5±8.1	协同
0.01	13.5±7.5	-1.4±2.3	16.4±4.1	协同	1.00	13.5±7.6	-0.4±1.5	10.3±6.3	拮抗
0.02	12.4±3.3	-2.5±2.1	11.8±8.8	协同	2.50	11.8±6.6	-0.5±1.5	13.4±7.9	协同
0.04	11.9±6.4	-2.7±3.8	9.4±5.8	协同	5.00	11.0±6.5	-1.4±1.2	11.2±8.1	协同
0.10	8.0±4.7	-2.4±9.0	6.5±3.2	协同	10.00	11.3±6.2	-1.1±1.4	10.2±6.1	协同
0.20	10.9±6.3	-1.7±1.3	9.7±7.5	协同	13.00	12.7±8.8	-1.2±1.0	11.9±5.1	协同
0.30	12.4±8.2	-1.6±0.9	10.6±6.3	拮抗	20.00	10.8±6.4	-1.0±1.1	9.7±5.9	拮抗
0.40	13.0±6.8	-0.3±4.9	11.8±5.9	拮抗	30.00	11.9±6.5	-1.6±2.2	9.8±7.9	拮抗

C源与NP添加对Cd胁迫下林地土壤呼吸作用的影响

Effects of C, N and P additions on soil respiration in woodland under Cd stress

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