当年和越年生香榧叶片的光合效率及抗氧化特性的季节性变化

doi:10.12302/j.issn.1000-2006.202204017

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南京林业大学学报（自然科学版） ›› 2024, Vol. 48 ›› Issue (2): 45-50.doi: 10.12302/j.issn.1000-2006.202204017

• 专题报道Ⅱ: 乡村振兴视域下特色林果培育专题（执行主编李维林方升佐） • 上一篇下一篇

当年和越年生香榧叶片的光合效率及抗氧化特性的季节性变化

赵晓龙¹(), 沈家怡¹, 刘涛², 吴家胜¹, 胡渊渊¹^,^*()

1.亚热带森林培育国家重点实验室,浙江农林大学林业与生物技术学院,浙江杭州 311300
2.杭州市原种场,浙江杭州 311115

收稿日期:2022-03-04 修回日期:2022-04-18 出版日期:2024-03-30 发布日期:2024-04-08
通讯作者: *胡渊渊(hyy_1985@zafu.edu.cn),教授。
作者简介:赵晓龙(zhaoxiaolong199860@qq.com)。
基金资助:
国家自然科学基金区域创新发展联合基金(U20A2049);国家自然科学面上基金项目(32271922)

Seasonal variations in photosynthetic efficiency and antioxidant characteristics of the current and one year-old leaves in Torreya grandis‘Merrillii’

ZHAO Xiaolong¹(), SHEN Jiayi¹, LIU Tao², WU Jiasheng¹, HU Yuanyuan¹^,^*()

1. State Key Laboratory of Subtropical Silviculture, College of Forestry and Biotechnology, Zhejiang A & F University, Hangzhou 311300, China
2. Hangzhou Raw Seed Growing Farm, Hangzhou 311115, China

Received:2022-03-04 Revised:2022-04-18 Online:2024-03-30 Published:2024-04-08

摘要/Abstract

摘要：

【目的】通过比较分析香榧(Torreya grandis ‘Merrillii’)主要功能叶的叶绿素荧光参数、抗氧化酶活性及丙二醛(MDA)含量等参数的季节性变化,初步探讨香榧叶片光合特性对高温、低温的温度变化的适应机制。【方法】以人工林中16年生香榧的当年生叶片和越年生叶片为研究对象,测定5、6、8、11月和次年1月中旬香榧叶片的叶绿素荧光参数、比叶质量(SLW)、抗氧化酶活性及可溶性蛋白含量的变化。【结果】 ①与5月中旬相比,8月中旬香榧当年生和越年生叶片光系统Ⅱ(PSⅡ)的实际最大光化学量子效率(Φ_PSⅡ)均明显上调,而PSⅡ处调节的能量耗散量子效率(Φ_NPQ)均明显下调,其PSⅡ处非调节性能量热耗散量子效率(Φ_NO)未发生明显变化;香榧当年生叶片的超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性均明显增强,过氧化物酶(POD)活性则明显减弱,丙二醛(MDA)含量则无明显变化。②与11月中旬相比, 次年1月的平均温度约降低了近10 ℃,香榧当年生和越年生叶片的Φ_PSⅡ和Φ_NPQ均显著降低,而其Φ_NO呈明显增加趋势,且MDA含量均呈显著增加趋势。【结论】与5月中旬相比,8月中旬温度较高,香榧叶片可以吸收较多光能,并用于电子传递,以热的形式耗散能量较少,其光合机构未发生光抑制,其较强的抗氧化酶体系能协调地助其适应高温高光环境,因此其当年生叶片和越年生叶片的Φ_PSⅡ明显增强;与11月中旬相比,次年1月中旬香榧当年生叶片和越年生叶片在越冬期间受到了冷害,叶片光合机构受到光损伤。综上所述,香榧叶片对环境表现为耐热不耐冷,这可能是其分布北扩的限制因素。

关键词: 香榧, 叶龄, 抗氧化酶, 光合特性, 季节性变化

Abstract:

【Objective】 Determination of seasonal changes in chlorophyll fluorescence parameters, antioxidant enzymes, malondialdehyde (MDA) and other parameters in current and 1 year-old leaves of Torreya grandis ‘Merrillii’ was to study the adaptive mechanism of the photosynthetic characteristics of T. grandis ‘Merrillii’ leaves to temperature changes during season. 【Method】 The chlorophyll fluorescence parameters, specific leaf weight (SLW), antioxidant enzyme activity and soluble protein changes of T. grandis ‘Merrillii’ leaves were measured in May, June, August, November and January of the second year. 【Result】 (1) Compared with mid-May, Φ_PSⅡ of T. grandis ‘Merrillii’ leaves in mid-August was significantly up-regulated, while Φ_NPQ was significantly down-regulated, and Φ_NOwith little change. SOD and CAT activities in T. grandis ‘Merrilli’ current leaves were significantly increased, POD activity was significantly decreased, and MDA with little change.(2) Compared with mid-November, the average temperature in January of the second year decreased by nearly 10 ℃. Φ_PSⅡ and Φ_NPQ of T. grandis ‘Merrillii’ current leaves and one-year old leaves decreased significantly, while their Φ_NOand MDA content increased significantly. 【Conclusion】 Compared with mid-May, T. grandis ‘Merrillii’ current leaves and one-year old leaves markedly improved Φ_PSⅡ in mid-August is due to the high temperature and its leaves to absorb more light, and used for electron transfer, less dissipation of energy in the form of heat, the photosynthetic photoinhibition not occurring, its strong antioxidant enzyme system can coordinate to help them adapt to the high temperature specular environment. Compared with mid-November, T. grandis ‘Merrillii’ current leaves and one-year old leaves suffered cold damage during overwintering in mid-January of the second year, and the photosynthetic organs of leaves suffered light damage. Above all, T. grandis ‘Merrillii’ leaves are resistant to heat weather but not to cold weather, which may be the limiting factor of northern expansion.

Key words: Torreya grandis ‘Merrillii’, different leaf age, antioxidant enzymes, photosynthetic characteristics, seasonal change

中图分类号:

S791.43

赵晓龙,沈家怡,刘涛,等. 当年和越年生香榧叶片的光合效率及抗氧化特性的季节性变化[J]. 南京林业大学学报（自然科学版）, 2024, 48(2): 45-50.

ZHAO Xiaolong, SHEN Jiayi, LIU Tao, WU Jiasheng, HU Yuanyuan. Seasonal variations in photosynthetic efficiency and antioxidant characteristics of the current and one year-old leaves in Torreya grandis‘Merrillii’[J].Journal of Nanjing Forestry University (Natural Science Edition), 2024, 48(2): 45-50.DOI: 10.12302/j.issn.1000-2006.202204017.

图/表 4

参考文献 25

[10]	HUANG Z G, YU W W, LUO H H, et al. Photosynthetic characteristics and their relationships with leaf nitrogen content and nitrogen allocation in leaves at different leaf age[J]. Sci Silvae Sin, 2015, 51(2): 44-51. DOI: 10.11707/j.1001-7488.20150206.
[11]	HU Y Y, ZHANG Y L, YU W W et al. Novel insights into the influence of seed sarcotesta photosyntheis on accumulation of seed dry matter and oil content in Torreya grandis cv. ‘Merrillii’[J]. Front Plant Sci, 2018, 8: 2179. DOI: 10.3389/fpls.2017.02179.
[12]	靳川, 查天山, 贾昕, 等. 毛乌素沙地少柳光系统Ⅱ光保护机制和能量分配动态及其影响因子[J]. 林业科学, 2020, 56(10): 34-44.
	JIN C, ZHA T S, JIA X, et al. Light energy partitioning, photoprotection and influencing factors of photosystem Ⅱ in an exotic species (Salix psammophila) in Mu us sandy land[J]. Sci Silvae Sin, 2020, 56(10): 34-44. DOI: 10.11707/j.1001-7488.20170105.
[13]	LI T T, HU Y Y, DU X H, et al. Salicylic acid alleviates the adverse effects of salt stress in Torreya grandis cv. Merrillii seedlings by activating photosynthesis and enhancing antioxidant systems[J]. PLoS One, 2014, 9(10): e109492. DOI: 10.1371/journal.pone.0109492.
[14]	张志良, 瞿伟菁, 李小方. 植物生理学实验指导[M]. 北京: 高等教育出版社, 2009.
	ZHANG Z L, QU W J, LI X F. Experimental instruction of plant physiology[M]. Beijing: Higher Education Press, 2009.
[15]	BRADFORD M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Anal Biochem, 1976, 72 (1/2): 248-254. DOI: 10.1016/0003-2697(76)90527-3.
[16]	冯玉龙, 曹坤芳, 冯志立, 等. 四种热带雨林树种幼苗比叶重, 光合特性和暗呼吸对生长光环境的适应[J]. 生态学报, 2002, 22(6): 901-910.
	FENG Y L, CAO K F, FENG Z L, et al. Acclimation of Lamina mass per unit area,photosynthetic characteristics and dark respiration to growth light regimes in four tropical rainforest species[J]. Acta Ecol Sin, 2002, 22(6): 901-910. DOI: 10.3321/j.issn:1000-0933.2002.06.015.
[17]	郝向春, 周帅, 翟瑜, 等. 温度变化对南极假山毛榉光合系统的影响[J]. 中南林业科技大学学报. 2019, 39(9):1-7.
[1]	陈力耕, 王辉, 童品璋. 香榧的主要品种及其开发价值[J]. 中国南方果树, 2005, 34(5): 33-34.
	CHEN L G, WANG H, TONG P Z. The main varieties of Torreya grandis and its development value[J]. South China Fruits, 2005, 34(5): 33-34. DOI: 10.3969/j.issn.1007-1431.2005.05.017.
[2]	黎章矩, 程晓建, 戴文圣, 等. 浙江香榧生产历史、现状与发展[J]. 浙江林学院学报, 2005, 21(4):471-474.
	LI Z J, CHENG X J, DAI W S, et al. History and status and development of Torreya grandis in Zhejiang Province[J]. J Zhejiang For Coll, 2004, 21(4): 471-474. DOI: 10.3969/j.issn.2095-0756.2004.04.023.
[3]	WU Z M, ZHANG X, WANG J L, et al. Leaf chloroplast ultrastructure and photosynthetic properties of a chlorophyll-deficient mutant of rice[J]. Photosynthetica, 2014, 52(2): 217-222. DOI: 10.1007/s11099-014-0025-x.
[4]	周振翔, 李志康, 陈颖, 等. 叶绿素含量降低对水稻叶片光抑制与光合电子传递的影响[J]. 中国农业科学, 2016, 49(19): 3709-3720.
	ZHOU Z X, LI Z K, CHEN Y, et al. Effects of reduced chlorophyll content on photoinhibition and photosynthetic electron transport in rice leaves[J]. Sci Agric Sin, 2016, 49(19): 3709-3720. DOI: 10.3864/j.issn.0578-1752.2016.19.004.
[5]	尹永强, 胡建斌, 邓明军. 植物叶片抗氧化系统及其对逆境胁迫的响应研究进展[J]. 中国农学通报, 2007, 23(1): 115-120.
	YIN Y Q, HU J B, DENG M J. Latest development of antioxidant system and responses to stress in plant leaves[J]. Chinese Agri Sci Bull, 2007, 23(1) : 115-120.
[6]	陈婷婷, 符卫蒙, 余景, 等. 彩色稻叶片光合特征及其与抗氧化酶活性、花青素含量的关系[J]. 中国农业科学, 2022, 55(3): 467-478.
	CHEN T T, FU W M, YU J, et al. The photosynthesis characteristics of colored rice leaves and its relation with antioxidant capacity and anthocyanin content[J]. Sci Agric Sin. 2022, 55(3): 467-478. DOI: 10.3864/j.issn.0578-1752.2022.03.004.
[17]	HAO X C, ZHOU S, ZHAI Y, et al. Influence of temperature stress on photosystem of Nothofagus antarctica[J]. J Central South Univ Forestry Technol, 2019, 39(9):1-7. DOI: 10.14067/j.cnki.1673-923x.2019.09.001.
[18]	徐超, 王明田, 杨再强, 等. 高温对温室草莓光合生理特性的影响及胁迫等级构建[J]. 应用生态学报, 2021, 32(1): 231-240.
	XU C, WANG M T, YANG Z Q, et al. Effects of high temperature on photosynthetic physiological characteristics of strawberry seedlings in greenhouse and construction of stress level[J]. Chin J Appl Ecol, 2021, 32(1):231-240.DOI: 10.13287/j.1001-9332.202101.028.
[19]	ISHIDA A, YAMAZAKI J Y, HARAYAMA H, et al. Photoprotection of evergreen and drought-deciduous tree leaves to overcome the dry season in monsoonal tropical dry forests in Thailand[J]. Tree Physiol, 2014, 34(1): 15-28. DOI: 10.1093/treephys/tpt107.
[20]	孙猛, 吕德国, 刘威生. 不同品种群15个杏品种荧光特性研究初探[J]. 中国农学通报, 2010, (6): 171-176.
	SUN M, LYU D G, LIU W S. Studies on the chlorophyll fluorescent characteristics of 15 apricot cultivars among different variety groups[J]. Chin Agric Sci Bull, 2011, 27(6)171-176.DOI: 10.3969/j.issn.1002-2910.2006.03.001.
[21]	UZILDAY B, TURKAN I, SEKMEN A H, et al. Comparison of ROS formation and antioxidant enzymes in Cleome gynandra (C₄) and Cleome spinosa (C₃) under drought stress[J]. Plant Sci, 2012, 182:59-70.DOI: 10.1016/j.plantsci.2011.03.015.
[22]	张强, 郭传友, 张兴旺, 等. 基于光合作用和抗氧化机制的南方铁杉和褐叶青冈越冬策略研究[J]. 植物研究, 2015, 35(2):200-207.
	ZHANG Q, GUO C Y, ZHANG X W, et al. Photosynthesis and antioxidant defense strategies in overwintering plants of Tsuga chinensis and Cyclobalanopsis stewardiana[J]. Bull Bot Res, 2015, 35(2):200-207.DOI: 10.7525/j.issn.1673-5102.2015.02.007.
[23]	王丹, 宣继萍, 郭海林, 等. 结缕草的抗寒性与体内碳水化合物、脯氨酸、可溶性蛋白季节动态变化的关系[J]. 草业学报, 2011, 20(4): 98-107.
	WANG D, XUAN J P, GUO H L, et al. Seasonal changes of freezing tolerance and its relationship to the contents of carbohydrates, proline, and soluble protein of Zoysia[J]. Acta Prataculturae Sin, 2011, 20(4): 98-107.
[7]	刘春燕, 周龙, 贾舟楫, 等. 黄化对吐鲁番葡萄叶片光合及叶绿素荧光特性的影响[J]. 经济林研究, 2018, 36(2): 115-120.
	LIU C Y, ZHOU L, JIA Z J, et al. Effects of chlorosis on photosynthetic and chlorophyll fluorescence characteristics of Turpan grape leaves[J]. Nonwood For Res, 2018, 36(2): 115-120. DOI: 10.14067/j.cnki.1003-8981.2018.02.017.
[8]	黄小辉, 吴焦焦, 王玉书, 等. 不同供氮水平核桃的生长及叶绿素荧光特性[J]. 南京林业大学学报, 2022, 46(2): 119-126.
	HUANG X H, WU J J, WANG Y S, et al. Growth and chlorophyll fluorescence characteristics of walnut (Juglans regia) seedling under different nitrogen supply levels[J]. J Nanjing For Univ (Nat Sci Ed), 2022, 46(2): 119-126. DOI: 10.12302/j.issn.1000-2006.202104016.
[9]	董斌, 蓝来娇, 黄永芳, 等. 干旱胁迫对油茶叶片叶绿素含量和叶绿素荧光参数的影响[J]. 经济林研究, 2020, 38(3):16-25.
	DONG B, LAN L J, HUANG Y F, et al. Effects of drought stress on photosynthetic pigments and chlorophyll fluorescence characteristics in leaves of Camellia oleifera[J]. Nonwood For Res, 2020, 38(3): 16-25. DOI: 10.14067/j.cnki.1003-8981.2020.03.003.
[10]	黄增冠, 喻卫武, 罗宏海, 等. 香榧不同叶龄叶片光合能力与氮含量及其分配关系的比较[J]. 林业科学, 2015, 51(2): 44-51.
[24]	王泽华, 秦伟. 不同居群新疆野苹果生理响应差异与抗寒性的关系[J]. 经济林研究, 2018, 36(2): 20-28.
	WANG Z H, QIN W. Study on the relationship between physiological response and cold hardiness of Malus sieversii in different populations[J]. Nonwood For Res, 2018, 36(2): 20-28.
[25]	CATONI R, GRATANI L. Variations in leaf respiration and photosynthesis ratio in response to air temperature and water availability among Mediterranean evergreen species[J]. J Arid Environ, 2014, 102: 82-88. DOI: 10.1016/j.jaridenv.2013.11.013.

月份 month	平均最高气温 mean max air temperature	平均最低气温 mean min air temperature
5	28.06±3.63 b	18.26±2.00 b
6	27.83±2.41 b	21.40±1.55 b
8	35.06±2.10 a	26.71±1.04 a
11	17.80±2.33 c	11.37±2.74 c
1	8.09±4.25 d	1.81±3.64 d

当年和越年生香榧叶片的光合效率及抗氧化特性的季节性变化

Seasonal variations in photosynthetic efficiency and antioxidant characteristics of the current and one year-old leaves in Torreya grandis‘Merrillii’

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