基于高通量测序技术的松材线虫研究进展

doi:10.12302/j.issn.1000-2006.202204039

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南京林业大学学报（自然科学版） ›› 2022, Vol. 46 ›› Issue (4): 1-7.doi: 10.12302/j.issn.1000-2006.202204039

基于高通量测序技术的松材线虫研究进展

丁晓磊(), 张悦, 林司曦, 叶建仁()

南京林业大学,南方现代林业协同创新中心,南京林业大学林学院, 江苏南京 210037

收稿日期:2022-04-08 修回日期:2022-05-10 出版日期:2022-07-30 发布日期:2022-08-01
通讯作者: 叶建仁
基金资助:
国家重点研发计划(2021YFD1400903);国家自然科学基金青年项目(31800543)

An overview of high-throughput sequencing techniques applied on Bursaphelenchus xylophilus

DING Xiaolei(), ZHANG Yue, LIN Sixi, YE Jianren()

Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China

Received:2022-04-08 Revised:2022-05-10 Online:2022-07-30 Published:2022-08-01
Contact: YE Jianren

摘要/Abstract

摘要：

松材线虫(Bursaphelenchus xylophilus)是松树萎蔫病的病原,严重威胁欧亚等国的松林资源和生态安全。自2011年松材线虫基因组首次公布以来,高通量测序技术成为该病害的重要研究手段之一。笔者从松材线虫的功能基因、非编码RNA、转录表达差异和基因组学等方面综述其致病机理,认为随着高通量测序技术的快速发展,在转录组相关研究方面,可尝试开展空间转录组、单细胞测序等新技术,在染色体基因组的支持下寻找松材线虫中具有潜在调控功能的长链非编码RNA、融合基因和环状RNA等罕见核酸分子,挖掘可能存在的甲基化、RNA编辑、结构变异等未知现象;在基因组学研究方面,可以开展基因家族扩增、大样本全基因组关联分析和数量性状定位等研究,以挖掘与松材线虫致病力和繁殖力等重要性状紧密关联的基因或位点,阐明松材线虫不同种群在进化过程中发生的适应性变化。

关键词: 松材线虫, 高通量测序, 转录组学, 基因组学

Abstract:

As of 2022, four decades have elapsed since the pine wilt disease entered China. The disease caused by the pine wood nematode (Bursaphelenchus xylophilus) poses a major threat to all pine forests in Asia and Europe. Since the announcement of the first B. xylophilus genome assembly in 2011, high-throughput sequencing techniques have been widely used in all aspects of relevant studies. They have facilitated researchers greatly in further exploring the molecular pathogenesis of B. xylophilus from gene functions, non-coding RNA, transcriptomics and genomics. With rapid developments in sequencing techniques, we suggest researchers to use spatial transcriptomics and single cell sequencing methods for identifying long non-coding RNAs, fusion genes, circular RNAs, as well as gene methylations, RNA editings and structural variations with potential regulatory roles. As for genomics, future studies could focus on gene family expansion, genome wide association and quantitative trait locus studies to screen the gene and loci highly associated with the pathogenicity and reproductivity of B. xylophilus and reveal the adaptation mechanisms among different nematode populations during the evolution.

Key words: Bursaphelenchus xylophilus, high-throughput sequencing, transcriptomics, genomics

中图分类号:

S763

丁晓磊,张悦,林司曦,等. 基于高通量测序技术的松材线虫研究进展[J]. 南京林业大学学报（自然科学版）, 2022, 46(4): 1-7.

DING Xiaolei, ZHANG Yue, LIN Sixi, YE Jianren. An overview of high-throughput sequencing techniques applied on Bursaphelenchus xylophilus[J].Journal of Nanjing Forestry University (Natural Science Edition), 2022, 46(4): 1-7.DOI: 10.12302/j.issn.1000-2006.202204039.

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基于高通量测序技术的松材线虫研究进展

An overview of high-throughput sequencing techniques applied on Bursaphelenchus xylophilus

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