油松抗性相关激素与代谢物对油松毛虫取食与剪叶刺激的响应

doi:10.12302/j.issn.1000-2006.202206014

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南京林业大学学报（自然科学版） ›› 2024, Vol. 48 ›› Issue (1): 219-226.doi: 10.12302/j.issn.1000-2006.202206014

油松抗性相关激素与代谢物对油松毛虫取食与剪叶刺激的响应

赵亚楠(), 孙天骅, 王利峰, 许强, 刘军侠, 高宝嘉, 周国娜()

河北农业大学林学院,河北保定 071000

收稿日期:2022-06-24 修回日期:2023-02-14 出版日期:2024-01-30 发布日期:2024-01-24
通讯作者: 周国娜
基金资助:
国家自然科学基金项目(30771739)

Plant hormones and metabolites response to feeding stimulation by pine caterpillar (Dendrolimus tabulaeformis) and leaf clipping control in Chinese pine (Pinus tabuliformis)

ZHAO Ya’nan(), SUN Tianhua, WANG Lifeng, XU Qiang, LIU Junxia, GAO Baojia, ZHOU Guona()

College of Forestry,Hebei Agricultural University,Baoding 071000,China

Received:2022-06-24 Revised:2023-02-14 Online:2024-01-30 Published:2024-01-24
Contact: ZHOU Guona

摘要/Abstract

摘要：

【目的】通过对油松毛虫(Dendrolimus tabulaeformis)取食刺激后的油松(Pinus tabuliformis)针叶进行分析,明确针叶树响应昆虫取食后树体内化合物的代谢途径变化,为油松的生长与保护提供参考。【方法】在河北省承德市平泉市黄土梁子林场选取生长状况良好的油松纯林,对距地面高度一致的4个方向针叶进行10头油松毛虫取食(feeding stimulation,FS)与剪叶刺激(leaf clipping control, LCC)处理,摘取取食点或剪叶点下方3 cm的油松针叶,对照组不做任何处理(记为处理0 h,CK),取完整松针,对不同处理后0、2 与8 h的松针进行代谢组分析和总类黄酮的验证,对0、0.5、1.0、1.5、2.0、4.0、6.0和8.0 h的松针进行茉莉酸(JA)、水杨酸(SA)、生长素(IAA)与脱落酸(ABA)相关植物激素含量的测定。【结果】通过代谢组学分析,FS处理后2.0 h与LCC处理后2.0 h的差异积累代谢物(different accumulated metabolites,DAMs)主要注释和富集的前3条通路为类黄酮生物合成、氨基酸合成代谢、精氨酸和脯氨酸代谢;FS处理后8.0 h与LCC处理后8.0 h的DAMs主要注释和富集的前3条通路为类黄酮生物合成、亚油酸生物合成、黄酮和黄酮醇的生物合成。因此,与LCC处理相比,FS处理可以显著诱导油松针叶取食点处类黄酮类物质的表达上调。植物激素JA、IAA表现出与代谢组中相应物质一致的表达趋势。相关性分析显示,JA与SA、JA与IAA、IAA与ABA之间呈显著正相关关系(P<0.05),SA与IAA和ABA之间呈负相关关系。【结论】类黄酮途径是参与油松抗性形成的主要代谢途径之一,单纯的机械损伤不会引起JA、IAA与ABA的显著差异,且JA、IAA、ABA参与油松抗性形成与生长发育过程。

关键词: 油松, 油松毛虫, 取食刺激, 诱导抗性, 植物激素, 类黄酮途径, 代谢组学

Abstract:

【Objective】This experiment was conducted with the aim of defining changes in metabolic pathways in response to mandibulate insect feeding and providing references for the growth and protection of Chinese pine (Pinus tabuliformis).【Method】Pure P. tabuliformis forest in good growth status in Huangtuliangzi Forest Farm of Pingquan City, Chengde City, Hebei Province was the experimental field. Branches with consistent branch length and height above the ground in four directions were stimulated by ten pine caterpillars (Dendrolimus tabulaeformis) for feeding stimulation and leaf clipping control for mechanical damage. The pine needles were collected 3 cm below the chewing or cutting sites. The CK group received no treatment (0 h, recorded as 0 h), and 10 intact pine needles were collected. Metabolome and total flavonoid contents were measured in the needles at 0, 2 and 8 h after the different treatment modes. The plant hormones JA, SA, IAA and ABA were measured using needles at 0, 0.5, 1.0, 1.5, 2.0, 4.0, 6.0, and 8.0 h after different treatments.【Result】Analysis of metabolomics showed that the top three pathways mainly annotated and enriched for DAMs compared to 2.0 h after FS and LCC are flavonoid biosynthesis, amino acid metabolism, and arginine and proline metabolism; the top three pathways mainly annotated and enriched for DAMs compared to 8.0 h after FS and LCC are flavonoid biosynthesis, linoleic acid metabolism, and flavonoid and flavonol biosynthesis. Thus, D. tabulaeformis feeding stimulation can significantly induce the upregulation of flavonoid expression at the chewing sites of needles when compared to the leaf clipping control. The plant hormones, JA and IAA, showed expression trends consistent with those of the corresponding substances in the metabolome. A significant positive correlation between JA and SA, JA and IAA and IAA and ABA (P< 0.05) was observed. 【Conclusion】Thus, the flavonoid pathway is one of the main pathways involved in resistant formation in conifers. Simple damage mechanisms did not induce significant differences in JA, IAA and ABA; thus, JA, IAA and ABA are involved in the development of resistance and growth during biotic stress.

Key words: Pinus tabuliformis, Dendrolimus tabulaeformis, feeding stimulation, induced resistant, plant hormone, flavonoid pathway, metabolome

中图分类号:

S718
S763

赵亚楠,孙天骅,王利峰,等. 油松抗性相关激素与代谢物对油松毛虫取食与剪叶刺激的响应[J]. 南京林业大学学报（自然科学版）, 2024, 48(1): 219-226.

ZHAO Ya’nan, SUN Tianhua, WANG Lifeng, XU Qiang, LIU Junxia, GAO Baojia, ZHOU Guona. Plant hormones and metabolites response to feeding stimulation by pine caterpillar (Dendrolimus tabulaeformis) and leaf clipping control in Chinese pine (Pinus tabuliformis)[J].Journal of Nanjing Forestry University (Natural Science Edition), 2024, 48(1): 219-226.DOI: 10.12302/j.issn.1000-2006.202206014.

图/表 6

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物质名称 compound	0 h和FS 2 h 0 h vs FS 2 h	0 h和FS 8 h 0 h vs FS 8 h	FS 2 h和LCC 2 h FS 2 h vs LCC2 h	FS 8 h和LCC 8 h FS 8 h vs LCC8 h
异樱花素isosakuranetin	-2.20	0.00	1.46	0.00
山柰酚kaempferol	-1.54	0.00	-1.28	0.00
短叶松素pinobanksin	1.52	1.32	-1.26	0.00
二氢杨梅素(蛇葡萄素)dihydromyricetin	8.04	8.58	-5.82	-2.44
柚皮素naringenin	0.00	0.00	-1.50	-15.23
香橙素(二氢山柰酚) dihydrokaempferol	0.00	1.33	-1.13	-2.28
球松素pinostrobin	0.00	0.00	-6.03	-6.89
柚皮素查尔酮naringenin chalcone	1.61	1.45	-1.37	0.00
根皮素phloretin	2.51	1.98	-1.76	0.00
圣草酚eriodictyol	3.78	3.68	-3.29	-3.05
紫铆素butin	1.52	1.95	-1.67	-2.38
二氢槲皮素(花旗松素)dihydroquercetin	7.03	7.13	-6.57	-3.06
白杨素chrysin	0.00	0.00	0.00	-1.48
乔松素pinocembrin	-1.12	0.00	0.00	-8.03
橙皮素hesperetin	0.00	1.18	0.00	-1.49
新橙皮苷neohesperidin	0.00	0.00	0.00	-1.08
黄颜木素fustin	-1.23	0.00	0.00	1.24
3-O-乙酰短叶松黄烷酮pinobanksin 3-O-acetate	0.00	0.00	0.00	-1.30
槲皮素quercetin	0.00	0.00	0.00	0.00

油松抗性相关激素与代谢物对油松毛虫取食与剪叶刺激的响应

Plant hormones and metabolites response to feeding stimulation by pine caterpillar (Dendrolimus tabulaeformis) and leaf clipping control in Chinese pine (Pinus tabuliformis)

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