元宝枫嫩枝扦插技术及生根过程的生理生化分析

doi:10.12302/j.issn.1000-2006.202208015

国家林草科技领军期刊
中国精品科技期刊
中国高校百佳科技期刊
江苏省新闻出版政府奖期刊奖
RCCSE林学权威期刊（A+）
CSCD核心期刊
Scopus数据库收录期刊
中文核心期刊
SCD核心期刊

作者加群：102861116

微信公众号：南京林业大学学报

高级检索

南京林业大学学报（自然科学版） ›› 2024, Vol. 48 ›› Issue (5): 123-130.doi: 10.12302/j.issn.1000-2006.202208015

元宝枫嫩枝扦插技术及生根过程的生理生化分析

王玉虓¹(), 张斌², 马秋月³^,^*(), 付威¹, 康真⁴, 朱长红⁴, 李淑娴¹

1.南京林业大学林草学院,南方现代林业协同创新中心, 江苏南京 210037
2.甘肃省地质矿产勘查开发局第三地质矿产勘查院,甘肃兰州 730050
3.江苏省农业科学院,江苏南京 210014
4.襄阳市林业科学技术推广站,湖北襄阳 441022

收稿日期:2022-08-08 修回日期:2022-10-24 出版日期:2024-09-30 发布日期:2024-10-03
通讯作者: * 马秋月(yue.870808@163.com),副研究员。
作者简介:
王玉虓(wangyux1997@163.com)。
基金资助:
国家自然科学基金项目(32001357);国家自然科学基金项目(32471929);江苏省青年基金(面上项目)(BK20211139);江苏高校优势学科建设工程资助项目(PAPD)

Softwood cutting technology for Acer truncatum and physiological and biochemical analysis during rooting process

WANG Yuxiao¹(), ZHANG Bin², MA Qiuyue³^,^*(), FU Wei¹, KANG Zhen⁴, ZHU Changhong⁴, LI Shuxian¹

1. Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry and Grassland, Nanjing Forestry University, Nanjing 210037, China
2. The Third Geological and Mineral Exploration Institute of Gansu Bureau of Geology and Mineral Resources,Lanzhou 730050,China
3. Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
4. Forestry Science and Technology Spreading Station in Xiangyang, Xiangyang 441022, China

Received:2022-08-08 Revised:2022-10-24 Online:2024-09-30 Published:2024-10-03

摘要/Abstract

摘要：

【目的】研究不同处理对元宝枫嫩枝扦插生根的影响,筛选出适合元宝枫嫩枝扦插的最佳处理,分析营养物质含量、酶活性变化与插穗生根的关系,初步探究元宝枫的生根机理。【方法】以幼化和3年生的元宝枫母树为材料,分析生长调节剂(IBA、NAA、生根液)浓度、采穗部位(上部、下部)、幼化处理和扦插时间(6月和9月)等因素对元宝枫扦插生根的影响,调查生根率、愈伤率、根数、根长等生根指标,测定生根过程中营养物质(可溶性糖和可溶性蛋白)含量、氧化酶[过氧化物酶(POD)、多酚氧化酶(PPO)、吲哚乙酸氧化酶(IAAO)]活性的变化。【结果】IBA和NAA处理的最适质量浓度分别为500和800 mg/L,生根率分别为59.3%和52.7%;生根液处理的最适浓度为体积分数1.43%(稀释70倍),生根率为43.9%,三者之间差异显著,经生长调节剂处理后插穗各生根指标均优于对照。此外,6月扦插的生根效果优于9月,幼化处理插穗生根效果优于3年生母树,上部插穗扦插生根效果优于下部插穗,且上述处理均达到显著差异水平。插穗生根过程中,处理组可溶性糖含量的变化呈现先下降后上升的变化趋势,可溶性蛋白含量、POD及PPO活性整体呈先上升后下降的变化趋势;而IAAO活性呈下降—上升—下降的变化趋势。对照变化趋势与处理组大致相同,但处理组峰值出现时间均比对照提前了5~10 d。【结论】元宝枫不定根发生属于混合生根类型。幼化母树的上部插穗使用IBA 500 mg/L处理各生根指标均达到最优,生根率为81.3%。生长调节剂处理可以提高营养物质代谢速度和相关酶合成进程,对插穗生根有促进作用。

关键词: 元宝枫, 生长调节剂, 嫩枝扦插, 营养物质, 氧化酶

Abstract:

【Objective】 In order to investigate the effects of different treatments on the rooting of softwood cuttings of Acer truncatum and find the best treatment method. We analyzed the relationship between changes in nutrient content, oxidase activity, and rooting of cuttings, and preliminarily explored the rooting mechanism of A. truncatum. 【Method】 Using rejuvenated A. truncatum trees and three-year-old A. truncatum mother trees as study materials, we conducted an analysis of the effects of growth regulators [indole-3-butyric acid (IBA) and 1-naphthaleneacetic acid (NAA) and a rooting solution], cutting positions (upper and lower parts), rejuvenation, and cutting time (June and September) on the rooting of A. truncatum. Furthermore, we examined the rate of root growth, callus formation, number of roots, root length, and other indicators of root development. Additionally, changes in nutrient contents (soluble sugar and soluble protein) and changes in oxidase activity [peroxidase (POD), polyphenol oxidase (PPO), and indole-3-acetic acid oxidase (IAAO)] were measured during the rooting process. 【Result】 The optimum IBA and NAA concentrations were 500 and 800 mg/L respectively, and the rooting rates were 59.3% and 52.7%, respectively. The optimal concentration for the rooting solution was a 1.43% volume fraction (70-fold) dilution, which achieved a rooting rate of 43.9%. Significant differences were observed among the three treatments, with all rooting indexes of cuttings treated with growth regulators performed better than those of the control. Additionally, the rooting effect of cuttings in June was better than that in September; the rooting effect of rejuvenated mother trees was better than that of 3-year-old trees; and the rooting effect of upper cuttings was better than that of lower cuttings. There were substantial differences observed following these treatments. During the rooting of the cuttings, the changes in the soluble sugar content following the treatment displayed a decreasing trend followed by an increasing trend. The soluble protein content, POD activity, and PPO activity initially increased and then decreased. However, the IAAO activity displayed a trend of decrease-increase-decrease. The control group exhibited a similar changing trend to that of the treatment group, but the peak time for the treatment group was 5-10 days earlier than that of the control. 【Conclusion】 The adventitious root formation of A. truncatum was categorized as a mixed rooting type. When the upper cuttings of rejuvenated mother trees were treated with IBA 500 mg/L, all rooting indexes were optimized, and the rooting rate peaked at 81.3%. Growth regulator treatment can improve the metabolism rate of nutrients and the synthesis process of related oxidase, and has a facilitative effect on the rooting of cuttings.

Key words: Acer truncatum, growth regulator, softwood cutting, nutrient, oxidase

中图分类号:

S792.35

王玉虓,张斌,马秋月,等. 元宝枫嫩枝扦插技术及生根过程的生理生化分析[J]. 南京林业大学学报（自然科学版）, 2024, 48(5): 123-130.

WANG Yuxiao, ZHANG Bin, MA Qiuyue, FU Wei, KANG Zhen, ZHU Changhong, LI Shuxian. Softwood cutting technology for Acer truncatum and physiological and biochemical analysis during rooting process[J].Journal of Nanjing Forestry University (Natural Science Edition), 2024, 48(5): 123-130.DOI: 10.12302/j.issn.1000-2006.202208015.

图/表 7

表1

图1

表2

表3

表4

图2

图3

参考文献 34

[1]	傅立国. 中国高等植物-第七卷[M]. 青岛: 青岛出版社, 2001.
	FU L G. Higher plants of China:Vol. 7[M]. Qingdao: Qingdao Publishing House, 2001.
[2]	李悦, 蔡亚南, 任安琦, 等. 盐碱胁迫对元宝枫幼苗生长和生理特性的影响[J]. 东北林业大学学报, 2022, 50(8): 5-14,21.
	LI Y, CAI Y N, REN A Q, et al. Effects of saline-alkali stress on growth and physiological characteristics of Acer truncatum seedlings[J]. J Northeast For Univ, 2022, 50(8):5-14,21. DOI:10.13759/j.cnki.dlxb.2022.08.002.
[3]	王琨, 刘少波, 张娜, 等. 元宝枫研究进展[J]. 西北林学院学报, 2021, 36(3): 152-157,280.
	WANG K, LIU S B, ZHANG N, et al. Research progress on Acer truncatum[J]. J Northwest For Univ, 2021, 36(3): 152-157,280. DOI: 10.3969/j.issn.1001-7461.2021.03.23
[4]	王性炎, 王妹清. 新资源食品:元宝枫籽油[J]. 中国油脂, 2011, 36(9):56-59.
	WANG X Y, WANG M Q. New resource food: Acer truncatum seed oil[J]. China Oils Fats, 2011, 36(9): 56-59.
[5]	马秋月, 王亚楠, 李淑顺, 等. 元宝枫种子发育过程中油脂积累与可溶性糖、蛋白质之间的关系[J]. 江苏农业学报, 2021, 37(4): 982-989.
	MA Q Y, WANG Y N, LI S S, et al. The lipid accumulation and its relationship with soluble sugar and protein in Acer truncatum Bunge seeds development[J]. Jiangsu J Agric Sci, 2021, 37(4):982-989. DOI: 10.3969/j.issn.1000-4440.2021.04.022
[6]	MA Q Y, SUN T L, LI S S, et al. The Acer truncatum genome provides insights into nervonic acid biosynthesis[J]. Plant J, 2020, 104(3): 662-678. DOI: 10.1111/tpj.14954.
[7]	谷荣辉. 中国乡土树种元宝枫的化学成分及代谢组学研究[D]. 北京: 中央民族大学, 2019.
	GU R H. Chemical constituents and metabolomics of Acer truncatum, an arbor species native in China[D]. Beijing: Minzu University of China, 2019.
[8]	张洪波. 元宝枫优树繁育技术研究[D]. 北京: 北京林业大学, 2016.
	ZHANG H B. Study on regeneration technique of Acer truncatum elite trees[D]. Beijing: Beijing Forestry University, 2016.
[9]	赵瑞, 沈永宝. 林木扦插繁殖研究进展[J]. 种子, 2019, 38(9): 57-66.
	ZHAO R, SHENG Y B. Research advances in tree cutting propagation[J]. Seed, 2019, 38(9): 57-66. DOI: 10.16590/j.cnki.1001-4705.2019.09.057.
[10]	李岱龙, 王文文, 王娟, 等. 一种元宝枫扦插育苗方法. CN105830708A[P]. 2016.
	LI D L, WANG W W, WANG J, et al. A cutting seedling method of Acer truncatum. CN105830708A[P]. 2016.
[11]	施雷, 张超. 一种元宝枫树扦插方法. CN105993802A[P]. 2016.
	SHI L, ZHANG C. A cutting method of Acer truncatum. CN105993802A[P]. 2016.
[12]	李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000.
	LI H S. Principles and techniques of plant physiological and biochemical experiment[M]. Beijing: Higher Education Press, 2000.
[13]	高俊凤. 植物生理学实验指导[M]. 北京: 高等教育出版社, 2006.
	GAO J F. Experimental guidance for plant physiology[M]. Beijing: Higher Education Press, 2006.
[14]	贾娟, 姚延寿, 史敏华, 等. 生根剂促进槭树植物扦插繁殖的研究进展[J]. 西北林学院学报, 2010, 25(4): 107-109,134.
	JIA J, YAO Y S, SHI M H, et al. Advances in the researchers of rooting agent in cutting propagation of Aceraceae plants[J]. J Northwest For Univ, 2010, 25(4): 107-109,134.
[15]	郑巧巧, 兰思仁, 刘雪蝶, 等. 生长调节剂对‘香妃’含笑扦插生根及相关酶活性的影响[J]. 中南林业科技大学学报, 2020, 40(5): 67-76.
	ZHENG Q Q, LAN S R, LIU X D, et al. Effects of growth regulators on rooting and correlative enzyme activities of Michelia figo ‘Xiangfei’ cuttings[J]. J Central South Univ For Technol, 2020, 40(5): 67-76. DOI: 10.14067/j.cnki.1673-923x.2020.05.009.
[16]	王艺, 贾忠奎, 马履一, 等. 4种植物生长调节剂对红花玉兰嫩枝扦插生根的影响[J]. 林业科学, 2019, 55(7): 35-45.
	WANG Y, JIA Z K, MA L Y, et al. Effects of four plant growth regulators on rooting of the softwood cutting of Magnolia wufengensis[J]. Sci Silvae Sin, 2019, 55(7): 35-45. DOI:10.11707/j.1001-7488.20190704.
[17]	魏茂胜. 不同处理对茶绒杜鹃扦插生根与生长的影响[J]. 森林与环境学报, 2019, 39(1): 27-31.
	WEI M S. Effects of different treatments on the rooting and growth of Rhododendron apricum[J]. J For Environ, 2019, 39(1): 27-31. DOI:10.13324/j.cnki.jfcf.2019.01.005.
[18]	魏黔春, 江泽平, 刘建锋, 等. 侧柏古树扦插试验及插穗营养物质变化[J]. 南京林业大学学报(自然科学版), 2020, 44(1): 63-71.
	WEI Q C, JIANG Z P, LIU J F, et al. Effects of several factors on rooting of cutting propagation of ancient Platycladus orientalias and the changes of nutritive material[J]. J Nanjing For Univ (Nat Sci Ed), 2020, 44(1): 63-71. DOI:10.3969/j.issn.1000-2006.201903057.
[19]	卜晓婷, 乔孝禄, 徐晓华, 等. 母树年龄、采穗时期和抑制物质对纳塔栎生根的影响[J]. 东北林业大学学报, 2022, 50(2): 11-16,28.
	BU X T, QIAO X L, XU X H, et al. Effects of parent stock age, cutting time and rooting inhibitors on stem cutting of Quercus nuttallii[J]. J Northeast For Univ, 2022, 50(2): 11-16,28. DOI: 10.13759/j.cnki.dlxb.2022.02.013.
[20]	郑先波, 王昊, 谭彬, 等. 埋干幼化处理对榛扦插生根的影响[J]. 经济林研究, 2016, 34(2): 147-151.
	ZHENG X B, WANG H, TAN B, et al. Effects of rejuvenating treatments through burying trunk on cutting rooting in Corylus[J]. Nonwood For Res, 2016, 34(2): 147-151. DOI: 10.14067/j.cnki.1003-8981.2016.02.027.
[21]	袁利利. 华北五角枫扦插繁殖技术及生根机理的研究[D]. 泰安: 山东农业大学, 2012.
	YUAN L L. The studies on the propagation techniques and rooting mechanism of stem cuttings of Acer truncatum Bunge[D]. Tai’an: Shandong Agricultural University, 2012.
[22]	薛满满, 常雪薇, 李春明, 等. 美国白蜡嫩枝扦插生根特性[J]. 中南林业科技大学学报, 2022, 42(4): 40-48.
	XUE M M, CHANG X W, LI C M, et al. The rooting characteristics of Fraxinus americana softwood cuttings[J]. J Central South Univ For Technol, 2022, 42(4): 40-48. DOI: 10.14067/j.cnki.1673-923x.2022.04.005.
[23]	NEGISHI N, OISHI M, KAWAOKA A. Chemical screening for promotion of adventitious root formation in Eucalyptus globulus[J]. BMC Proc, 2011, 5(S7): 139. DOI: 10.1186/1753-6561-5-S7-P139.
[24]	侯江涛, 沈聪聪, 张毅芳, 等. 植物扦插繁殖生根机理研究综述[J]. 安徽农业科学, 2019, 47(19): 1-3,6.
	HOU J T, SHEN C C, ZHANG Y F, et al. Review on rooting mechanism of plant cuttings propagation[J]. J Anhui Agric Sci, 2019, 47(19): 1-3,6. DOI: 10.3969/j.issn.0517-6611.2019.19.001.
[25]	吕庚鑫, 孟益德, 庆军, 等. ‘华仲6号’杜仲嫩枝扦插生根的解剖及生理变化[J]. 林业科学, 2022, 58(2): 113-124.
	LV G X, MENG Y D, QING J, et al. Changes of anatomical structure and physiology during softwood cutting rooting of Eucommia ulmoides ‘Huazhong No. 6’[J]. Sci Silvae Sin, 2022, 58(2): 113-124. DOI: 10.11707/j.1001-7488.20220212.
[26]	RAPAKA V K, BESSLER B, SCHREINER M, et al. Interplay between initial carbohydrate availability, current photosynthesis and adventitious root formation in Pelargonium cuttings[J]. Plant Sci, 2005, 168(6): 1547-1560. DOI: 10.1016/j.plantsci.2005.02.006.
[27]	ZHANG J, SHI Y, ZHANG X Z, et al. Melatonin suppression of heat-induced leaf senescence involves changes in abscisic acid and cytokinin biosynthesis and signaling pathways in perennial ryegrass (Lolium perenne L.)[J]. Environ Exp Bot, 2017, 138: 36-45.DOI: 10.1016/j.envexpbot.2017.02.012.
[28]	梁玉堂, 龙庄如. 树木营养繁殖原理和技术[M]. 北京: 中国林业出版社, 1993.
	LIANG Y T, LONG Z R. Principles and techniques of vegetative propagation of trees[M]. Beijing: China Forestry Publishing House, 1993.
[29]	乔孝禄, 卜晓婷, 徐晓华, 等. 纳塔栎嫩枝扦插生根过程中的生理变化[J]. 中南林业科技大学学报, 2021, 41(4): 86-92.
	QIAO X L, BU X T, XU X H, et al. Rooting characteristics of Quercus nuttallii softwood cuttings and its physiological changes[J]. J Central South Univ For Technol, 2021, 41(4): 86-92. DOI: 10.14067/j.cnki.1673-923x.2021.04.010.
[30]	刘欢, 刘济明, 骆畅, 等. GGR-6对米槁插穗生根过程中生理动态的影响[J]. 东北林业大学学报, 2021, 49(10): 42-46,64.
	LIU H, LIU J M, LUO C, et al. Effect of GGR-6 on physiological dynamics of Cinnamomum migao H. W. Li cuttings during rooting[J]. J Northeast For Univ, 2021, 49(10): 42-46,64. DOI: 10.13759/j.cnki.dlxb.2021.10.008.
[31]	SOMKUWAR R G, BONDAGE D D, SURANGE M S, et al. Rooting behaviour, polyphenol oxidase activity, and biochemical changes in grape rootstocks at different growth stages[J]. Turkish J Agric For, 2011, 35(3): 281-287. DOI: 10.3906/tar-0911-62.
[32]	PEDREIRA J, TERESA HERRERA M, ZARRA I, et al. The overexpression of AtPrx37, an apoplastic peroxidase, reduces growth in Arabidopsis[J]. Physiol Plant, 2011, 141(2): 177-187. DOI: 10.1111/j.1399-3054.2010.01427.x.
[33]	凡莉莉, 薛磊, 赖金莉, 等. 大头典竹扦插过程中营养物质和氧化酶活性变化研究[J]. 竹子学报, 2018, 37(1): 54-59.
	FAN L L, XUE L, LAI J L, et al. Change of nutrient content and oxidase activities during cutting propagation of Dendrocalamopsis beecheyana[J]. J Bamboo Res, 2018, 37(1):54-59. DOI: 10.19560/j.cnki.issn1000-6567.2018.01.009.
[34]	吴红, 陆辉, 高克利, 等. 生长调节剂对青钱柳扦插生根及生根过程中保护酶系统的影响[J]. 北方园艺, 2019(20): 106-111.
	WU H, LU H, GAO K L, et al. Effects of growth regulators on rooting characteristics and protective enzyme system of Cyclocarya paliurus cuttings[J]. North Hortic, 2019(20): 106-111. DOI: 10.11937/bfyy.20191100.

生长调节剂 growth regulation	用量 concentration	处理时间 treatment time
对照CK	清水	—
吲哚丁酸IBA	300、500、700 mg/L	速蘸10 s
萘乙酸NAA	800、1 000、1 200 mg/L	速蘸10 s
生根液 rooting solution	体积分数3.33%、2.00%、1.43% (稀释30、50、70倍)	浸泡30 min

处理 treatment	用量 concentration	愈伤率/% callus rate	生根率/% rooting rate	平均根长/cm average root length	平均根数/条 mean number	根系指数 rooting index
	CK1	35.0±1.7 e	32.2±1.9 e	8.8±0.0 cd	1.7±0.1 cd	4.8±0.2 e
萘乙酸 NAA	800 mg/L	53.3±3.3 c	52.7±3.1 b	8.5±0.7 cd	1.9±0.1 abc	8.5±0.2 bc
	1 000 mg/L	64.8±1.8 a	46.4±3.4 cd	9.3±0.7 bc	2.1±0.1 a	9.1±1.0 b
	1 200 mg/L	58.4±2.4 b	44.0±4.0 d	7.9±0.3 d	1.8±0.1 bcd	5.8±0.3 de
吲哚丁酸 IBA	300 mg/L	46.2±2.7 d	50.6±4.2 bc	10.1±0.4 b	2.1±0.2 a	10.9±1.9 a
	500 mg/L	42.7±3.1 d	59.3±4.1 a	8.8±0.1 cd	1.8±0.1 bcd	9.6±0.6 ab
	700 mg/L	44.1±2.3 d	50.4±2.7 bc	9.1±0.0 c	2.1±0.1 a	9.7±0.2 ab
	CK2	45.0±1.7 d	30.3±2.5 e	8.4±0.2 cd	1.8±0.1 d	4.5±0.4 e
生根液体积分数 rooting solution valume fraction	3.33%(30倍)	47.3±4.4 d	36.1±2.8 e	11.7±1.2 a	1.7±0.1 d	7.0±1.3 cd
	2.00%(50倍)	62.7±2.5 ab	35.2±2.0 de	8.5±0.1 cd	2.0±0.2 ab	5.9±0.8 de
	1.43%(70倍)	65.0±5.0 a	43.9±3.5 d	10.1±0.6 b	2.1±0.2 a	9.3±1.1 ab

扦插时间 cutting time	愈伤率/% callus rate	生根率/% rooting rate	平均根长/cm average root length	平均根数/个 mean elements	根系指数 rooting index
6月16日	57.0±2.0 a	65.3±0.6 a	12.7±1.2 a	1.1±0.2 a	9.1±0.4 a
9月14日	43.0±3.0 b	47.7±2.1 b	8.8±0.1 b	0.6±0.0 b	2.5±0.1 b

采穗母树年龄 parent stock age	部位 position	愈伤率/% callus rate	生根率/% rooting rate	平均根长/cm average root length	平均根数/个 mean elements	根系指数 rooting index
幼化 rejuvenation	下部	66.3±1.5 b	72.3±2.1 b	12.3±1.1 a	1.3±0.1 b	11.6±0.3 b
	上部	74.3±3.5 a	81.3±2.1 a	13.0±1.0 a	1.6±0.1 a	16.9±0.3 a
3年生 three-year old	下部	56.7±1.5 c	45.7±2.0 d	8.3±0.6 b	1.1±0.1 c	4.2±0.2 d
	上部	60.7±0.6 c	64.3±1.2 c	9.3±0.6 b	1.1±0.2 c	6.6±0.2 c

元宝枫嫩枝扦插技术及生根过程的生理生化分析

Softwood cutting technology for Acer truncatum and physiological and biochemical analysis during rooting process

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 7

参考文献 34

相关文章 15

编辑推荐

Metrics

本文评价

作者

读者

审稿

[1]	李潘亭, 杜满义, 王玥, 裴顺祥, 辛学兵. 元宝枫幼苗生长及光合特性对水肥耦合的响应[J]. 南京林业大学学报（自然科学版）, 2024, 48(5): 113-122.
[2]	马坛, 田野, 王书军, 李文昊, 段启英, 张庆源. 不同性别南方型黑杨无性系叶片对土壤短期间歇性干旱的生理响应[J]. 南京林业大学学报（自然科学版）, 2024, 48(3): 172-180.
[3]	赵晓龙, 沈家怡, 刘涛, 吴家胜, 胡渊渊. 当年和越年生香榧叶片的光合效率及抗氧化特性的季节性变化[J]. 南京林业大学学报（自然科学版）, 2024, 48(2): 45-50.
[4]	宫楠, 祖鑫, 解志军, 朱长红, 李淑娴. 紫荆种子吸胀和层积过程中不同相态水分变化的核磁共振检测[J]. 南京林业大学学报（自然科学版）, 2023, 47(6): 42-50.
[5]	刘相泉, 赵仁菲, 朱艳芳, 邓仕明, 李吉涛, 邓志军. 复羽叶栾树植冠种子库种子活力变化机制[J]. 南京林业大学学报（自然科学版）, 2023, 47(2): 35-41.
[6]	王碧霞, 杜小奇, 邓燕, 蔡晓梅, 苏光灿. 凉山油橄榄主栽品种叶中营养物质和酚类含量的季节性变化[J]. 南京林业大学学报（自然科学版）, 2022, 46(4): 169-176.
[7]	邹雨婷, 朱铭玮, 李永荣, 翟金庭, 李淑娴. ‘凤丹’种子发育及其营养物质含量和相关酶活性的动态变化[J]. 南京林业大学学报（自然科学版）, 2021, 45(5): 62-70.
[8]	张琳, 程亚男, 张欣, 杨伟婷, 孔庆涛, 谢东锋. 两种植物生长调节剂对木槿插穗生根的影响[J]. 南京林业大学学报（自然科学版）, 2021, 45(3): 123-129.
[9]	李佳琦, 薛晓明, 高捍东. 桢楠种子脱水过程中的生理响应[J]. 南京林业大学学报（自然科学版）, 2021, 45(3): 130-136.
[10]	石欣隆, 杨月琴, 薛娴, 刘伟, 宋程威, 郭丽丽, 侯小改. 壳寡糖对干旱胁迫下‘凤丹’幼苗生长及生理特性的影响[J]. 南京林业大学学报（自然科学版）, 2021, 45(2): 120-126.
[11]	马秋月, 李倩中, 李淑顺, 朱璐, 颜坤元, 李淑娴, 张斌, 闻婧. 元宝枫组织培养及快速繁殖技术研究[J]. 南京林业大学学报（自然科学版）, 2021, 45(2): 220-224.
[12]	王改萍, 王良桂, 王晓聪, 张晨, 章雷, 刘彬. 楸树嫩枝扦插生根发育及根系特征分析[J]. 南京林业大学学报（自然科学版）, 2020, 44(6): 94-102.
[13]	魏黔春, 江泽平, 刘建锋, 史胜青, 赵秀莲, 常二梅. 侧柏古树扦插试验及插穗营养物质变化[J]. 南京林业大学学报（自然科学版）, 2020, 44(1): 63-71.
[14]	廖杨文科, 崔荣荣, 谢寅峰. 氰丙氨酸合酶在乙烯诱导杨树耐盐中的作用[J]. 南京林业大学学报（自然科学版）, 2019, 43(6): 137-142.
[15]	佘露露, 王伟娟, 宋静静, 卢存福, 陈玉珍. 低温驯化对西藏绵头雪莲愈伤组织抗冻性及抗氧化能力的影响[J]. 南京林业大学学报（自然科学版）, 2019, 43(5): 181-186.