BR对东京野茉莉种子中脂肪酸合成相关酶活性及油脂积累的影响

doi:10.12302/j.issn.1000-2006.202209026

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南京林业大学学报（自然科学版） ›› 2023, Vol. 47 ›› Issue (6): 35-41.doi: 10.12302/j.issn.1000-2006.202209026

所属专题： “攥紧中国种子”视域下的中国林草种业研究专题Ⅱ

• 专题报道Ⅰ：“攥紧中国种子”视域下的中国林草种业研究专题Ⅱ（执行主编施季森李维林） • 上一篇下一篇

BR对东京野茉莉种子中脂肪酸合成相关酶活性及油脂积累的影响

陈俊娜¹(), 王晓宇¹, 陈晨¹, 彭辉武², 陈娟², 黄卫和², 喻方圆¹^,^*()

1.南京林业大学林草学院,南方现代林业协同创新中心,江苏南京 210037
2.江西省萍乡市林业科学研究所,江西萍乡 337000

收稿日期:2022-09-13 修回日期:2022-10-13 出版日期:2023-11-30 发布日期:2023-11-23
通讯作者: *喻方圆(fyyu@njfu.com.cn),教授。
基金资助:
南京市产学研合作后补助项目(201722081);江苏高校优势学科建设工程资助项目(PAPD);江西省省级林业补助专项项目(2021)

Effects of BR on enzyme activities related to fatty acid synthesis and oil accumulation of Styrax tonkinensis seeds

CHEN Junna¹(), WANG Xiaoyu¹, CHEN Chen¹, PENG Huiwu², CHEN Juan², HUANG Weihe², YU Fangyuan¹^,^*()

1. College of Forestry and Grassland, Nanjing Forestry University, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing 210037, China
2. Pingxiang Institute of Forestry, Jiangxi Province, Pingxiang 337000, China

Received:2022-09-13 Revised:2022-10-13 Online:2023-11-30 Published:2023-11-23

摘要/Abstract

摘要：

【目的】探究油菜素内酯(BR)对越南安息香(东京野茉莉,Styrax tonkinensis)种子发育过程中脂肪酸合成相关酶活性及油脂积累的影响,为揭示外源油菜素内酯促进东京野茉莉种子脂肪酸合成和油脂积累的生理机制提供一定的理论和实践依据。【方法】以10年生东京野茉莉结实母树为研究对象,设置喷施4种浓度(1、5、10、20 μmol/L)油菜素内酯处理,记为BR1、BR5、BR10、BR20,以喷施蒸馏水为对照。分别于花后45、65、95和125 d实施喷施处理,于花后50、70、100和130 d取样,测定种实发育过程中的FAS(脂肪酸合成酶)、ACC(乙酰辅酶A羧化酶)、DGAT(二酰甘油酰基转移酶)活性,以及果实鲜质量和种子粗脂肪质量分数,分析不同浓度BR处理对东京野茉莉种实发育过程中脂肪酸合成相关酶活性及油脂积累的影响。【结果】BR处理对东京野茉莉种实发育过程中脂肪酸合成相关酶活性有显著影响。东京野茉莉种子FAS活性变化十分显著,在花后50 d,各处理间的FAS活性较低,随后上升,到花后100 d达到最大峰值,后缓慢下降。且在花后100 d,BR5和BR10处理的FAS活性同时达到最大值,都为对照组的1.8倍,活性分别为329.104和326.744 nmol/(min·mg)。东京野茉莉种子的ACC活性在发育期呈现“上升—下降—上升”的趋势。在花后70 d时,BR5处理的ACC活性值达到波峰位置,活性为20.50 nmol/mg,是对照组的5.9倍。在4个时期中不同处理的DGAT活性先上升后下降,在花后100 d达到最高峰,各BR处理明显高于对照组,其中BR5处理后的DGAT活性达到最大值,为6.03 nmol/mg,是对照组的1.2倍。BR处理对东京野茉莉种子FAS、ACC和DGAT活性总体上具有促进作用,以BR5处理效果最为显著。在4个时期中,均以中等浓度的BR处理促进东京野茉莉种子粗脂肪质量分数的效果最佳;在花后70 d,各处理下的种子粗脂肪质量分数存在显著性差异,BR5处理下的种子粗脂肪质量分数显著地高于其他处理。不同浓度的BR处理后,东京野茉莉果实鲜质量总体上有所增加。除花后100 d外,其他时期以BR5处理对果实鲜质量的增加效果最好。各处理的东京野茉莉种子脂肪酸合成相关酶活性与种子粗脂肪质量分数和果实鲜质量存在显著的正相关关系。【结论】FAS、ACC和DGAT活性动态变化表明,BR处理能够提高种子脂肪酸合成相关酶活性,促进种实发育过程中脂肪酸的合成。相关分析结果表明,东京野茉莉种子脂肪酸合成相关酶活性与果实鲜质量和种子粗脂肪质量分数存在显著正相关关系。

关键词: 油菜素内酯(BR), 东京野茉莉, 脂肪酸合成相关酶, 油脂积累

Abstract:

【Objective】The study explored the effects of brassinolide (BR) on enzyme activities related to fatty acid synthesis and oil accumulation in seeds of Styrax tonkinensis. The aim was to provide theoretical and practical data for studies of the mechanism by which exogenous BR promotes fatty acid synthesis and oil accumulation in seeds of S. tonkinensis. 【Method】Ten-year-old seed trees of S. tonkinensis were sprayed with four different concentrations of brassinolide solution: 1(BR 1), 5(BR 5), 10(BR 10) and 20(BR 20) μmol/L, with distilled water sprayed as the control. The different BR concentrations were sprayed 45, 65, 95 and 125 d after flowering. Fruit samples were collected 50, 70, 100 and 130 d after flowering. The activities of fatty acid synthase (FAS), 1-aminocyclopropane-1-carboxylate (ACC) and diacylglycerol acyltransferase (DGAT) were measured during seed development. Fruit fresh weight and seed crude fat mass fraction were also determined. The effects of different concentrations of BR on enzyme activities related to fatty acid synthesis in S. tonkinensis seeds were analyzed. 【Result】BR treatment had significant effects on the activities of enzymes related to fatty acid synthesis during the seed development of S. tonkinensis. FAS activity was low at 50 d after flowering, increased, significantly and peaked at 100 d after flowering, and then decreased slowly. At 100 d after flowering, the activity of FAS in the BR5 and BR10 treatments reached the maximum value at the same time. The respective values of 329.104 and 326.744 nmol/(min·mg) exceeded the control value by 1.8 times. The ACC activity of S. tonkinensis seeds increased, then decreased, then increased again. At 70 d after flowering, the ACC activity in the BR5 treatment peaked 20.50 nmol/mg, which was 5.9 times that of the control group. The activity of DGAT in the different treatments first increased and then decreased, reaching a peak at 100 d after flowering. The activity of DGAT in each BR treatment group was significantly higher than that in the control group. DGAT activity in the BR5 treatment was highest at 6.03 nmol/mg, which was 1.2 times of that the control group. BR promoted the activities of FAS, ACC and DGAT in S. tonkinensis seeds, with the most significant effect with the BR5 treatment. During the four periods of time, the medium concentration of BR treatment improved the crude fat fraction of S. tonkinensis seeds. Seventy days after flowering, there were significant differences in the seed crude fat mass fraction under different treatments, and that of the BR5 treatment was significantly higher than that of the other treatments. The fresh weight of S. tonkinensis fruits increased after treatment with different concentrations of BR. The BR5 treatment had the greatest effect on fruit fresh weight, except at 100 d after flowering. There was a significant positive correlation between enzyme activities related to fatty acid synthesis and both seed crude fat mass fraction and fruit fresh weight. 【Conclusion】 The dynamic changes in FAS, ACC and DGAT activities showed that BR treatment increased the activities of enzymes related to fatty acid synthesis and promoted fatty acid synthesis during seed development. The BR5 treatment had the best effect. BR treatment also increased fruit fresh weight and seed crude fat fraction. Correlation analysis showed that the activities of enzymes related to fatty acid synthesis in the seeds of S. tonkinensis were positively correlated with fruit fresh weight and seed crude fat mass fraction.

Key words: brassinolide (BR), Styrax tonkinensis, enzyme activities related to fatty acid synthesis, oil accumulation

中图分类号:

S722.1

陈俊娜,王晓宇,陈晨,等. BR对东京野茉莉种子中脂肪酸合成相关酶活性及油脂积累的影响[J]. 南京林业大学学报（自然科学版）, 2023, 47(6): 35-41.

CHEN Junna, WANG Xiaoyu, CHEN Chen, PENG Huiwu, CHEN Juan, HUANG Weihe, YU Fangyuan. Effects of BR on enzyme activities related to fatty acid synthesis and oil accumulation of Styrax tonkinensis seeds[J].Journal of Nanjing Forestry University (Natural Science Edition), 2023, 47(6): 35-41.DOI: 10.12302/j.issn.1000-2006.202209026.

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处理 treatments	鲜质量/mg fruit fresh mass
处理 treatments	50 d	70 d	100 d	130 d
CK	0.31±0.28 c	0.56±0.30 b	0.67±0.31 c	0.66±0.19 d
BR1	0.40±0.78 b	0.63±0.36 b	0.70±0.14 bc	0.80±0.69 b
BR5	0.54±0.51 a	0.86±0.66 a	—	1.07±0.53 a
BR10	0.39±0.27 b	0.62±0.13 b	0.82±0.14 a	0.79±0.33 b
BR20	0.40±0.41 bc	0.55±0.54 b	0.72±0.21 b	0.70±0.16 c

指标 index	FAS	ACC	DGAT
果实鲜质量 fresh fruit mass	0.585^**	0.481^**	0.445^**
粗脂肪质量分数 mass fraction of crude fat	0.408^**	0.609^**	0.316^*

BR对东京野茉莉种子中脂肪酸合成相关酶活性及油脂积累的影响

Effects of BR on enzyme activities related to fatty acid synthesis and oil accumulation of Styrax tonkinensis seeds

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[1]	吴岐奎,吴亚丽,喻方圆. 不同家系东京野茉莉苗期生长的差异性[J]. 南京林业大学学报（自然科学版）, 2018, 42(02): 191-196.
[2]	徐丽萍,喻方圆. 东京野茉莉雌雄蕊的形态及显微结构[J]. 南京林业大学学报（自然科学版）, 2017, 41(02): 34-40.