两种丛枝菌根真菌复合接种对青山杨叶片抗美国白蛾的影响

方静; 张书曼; 严善春; 武帅; 赵佳齐; 孟昭军

doi:10.12302/j.issn.1000-2006.202209054

方静, 张书曼, 严善春, 武帅, 赵佳齐, 孟昭军

南京林业大学学报（自然科学版） ›› 2024, Vol. 48 ›› Issue (2) : 144-154.

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南京林业大学学报（自然科学版） ›› 2024, Vol. 48 ›› Issue (2) : 144-154. DOI: 10.12302/j.issn.1000-2006.202209054

研究论文

两种丛枝菌根真菌复合接种对青山杨叶片抗美国白蛾的影响

作者信息 +

Effects of the compound inoculation of two arbuscular mycorrhizal(AM) fungi on the resistance of Populus pseudo-cathayana × P. deltoides leaves to Hyphantria cunea

Author information +

文章历史 +

摘要

【目的】为研究青山杨叶部害虫生物防控新途径,探索丛枝菌根(arbuscular mycorrhiza,AM)真菌复合接种对青山杨(Populus pseudo-cathayana × P. deltoides)叶片抗美国白蛾的影响。【方法】于温室盆栽条件下对1年生青山杨扦插苗进行根内根孢囊霉(Rhizophagus intraradices, RI)和摩西斗管囊霉(Funneliformis mosseae,FM)单一及复合接种(M)处理,以不接种处理为对照(CK),测定对青山杨叶片次生代谢物质、防御酶和蛋白酶抑制剂方面等化学防御能力的影响,并以美国白蛾(Hyphantria cunea)幼虫为生物测定对象判断青山杨的抗虫效果。【结果】在120 d内M组的菌根侵染率、丛枝着生率和根内泡囊数均高于FM和RI组,同时,M组能在一定程度上提高青山杨叶片的化学防御能力,其中叶片总生物碱、纤维素含量和过氧化物酶(POD)、过氧化氢酶(CAT)、脂氧合酶(LOX)、多酚氧化酶(PPO)、胰凝乳蛋白酶抑制剂(CI)、胰蛋白酶抑制剂(TI)活性显著高于RI、FM和CK组(P<0.05)。取食M组叶片的3龄美国白蛾幼虫取食量、排粪量、纤维素酶活性、乙酰胆碱酯酶(AchE)活性和多功能氧化酶(MFO)活性显著低于取食FM、RI和CK组的(P<0.05),3龄幼虫体长、食物利用率、胰蛋白酶活性、羧酸酯酶(CarE)活性、谷胱甘肽S-转移酶(GSTs)活性显著高于取食RI和FM组的(P<0.05),3龄幼虫食物消耗率和α-淀粉酶活性与RI组差异不显著,其体质量与取食FM组的差异不显著;取食M组叶片的4龄幼虫体质量、取食量、食物消耗率、纤维素酶活性、GSTs活性、CarE活性、AchE活性、MFO活性显著低于取食FM、RI和CK组的(P<0.05),4龄幼虫排粪量显著低于取食RI和CK组的(P<0.05),4龄幼虫胰蛋白酶活性显著高于其余3组(P<0.05),4龄幼虫的体长和食物利用率与取食RI组的差异不显著,4龄幼虫α-淀粉酶活性与取食FM和RI组的差异均不显著;取食M组叶片的5龄幼虫体长、体质量、取食量、排粪量、食物利用率、α-淀粉酶活性、纤维素酶活性、GSTs活性、CarE活性和MFO活性显著低于取食FM、RI和CK组的(P<0.05),5龄幼虫食物消耗率显著低于取食FM和RI组的(P<0.05),5龄幼虫AchE活性显著低于取食FM组的(P<0.05),5龄幼虫胰蛋白酶活性显著高于其余3组(P<0.05)。【结论】将RI与FM复合接种能够诱导青山杨叶片在次生代谢物质、防御酶和蛋白酶抑制剂方面的化学防御性能,RI和FM复合接种对青山杨的抗虫性表现优于RI、FM单一接种和无AM真菌接种,并且对美国白蛾幼虫具有一定的抑制效果,在实际应用时可优先考虑RI和FM复合接种。

Abstract

【Objective】 In order to study a new way of biological control of pests on Populus pseudo-cathayana × P. deltoides leaves, the effects of compound inoculation with arbuscular mycorrhizal (AM) fungi on the resistance of P. pseudo-cathayana×P. deltoides leaves to Hyphantria cunea were investigated. 【Method】 One-year-old P. pseudo-cathayana × P. deltoides cuttings were inoculated with Rhizophagus intraradices (RI) and Funneliformis mosseae (FM) by single and mixed inoculation (M) in a greenhouse, with no inoculation (CK) as the control. The chemical defense ability of secondary metabolites, defense enzymes and protease inhibitors in poplar leaves was determined, and the larva of H. cunea was used as a bioassay object to determine its anti-insect effect.【Result】 In 120 days, the mycorrhizal infection rate, arbuscular growth rate and number of vesicles in the root of the M group were higher than those of the FM and RI groups. Meanwhile, the M group could improve the chemical defense ability of the leaves of P. pseudo-cathayana × P. deltoides to a certain extent. The contents of total alkaloids and cellulose and the activities of peroxidase (POD), catalase (CAT), lipoxygenase (LOX), polyphenol oxidase (PPO), chymotrypsin inhibitor (CI) and trypsin inhibitor (TI) in leaves were significantly higher than those in the RI, FM and CK groups (P<0.05). The food intake, fecal output, cellulase activity, acetylcholinesterase (AchE) activity and multifunctional oxidase (MFO) activity of the third instar larvae in the M group were significantly lower than those in the FM, RI and CK groups (P<0.05). The body length, food availability, trypsin activity, carboxylesterase (CarE) activity, and glutathione S-transferase (GSTs) activity of the third instar larvae were significantly higher than those of the RI and FM groups (P<0.05). The food consumption rate and α-amylase activity of the third instar larvae did not significantly differ from those of the RI group, and the body weight of the third instar larvae did not significantly differ from those of the FM group. Body weight, food intake, food consumption rate, cellulase activity, GSTs activity, CarE activity, AchE activity and MFO activity of the fourth instar larvae were significantly lower than those of the FM, RI and CK groups (P<0.05), the fecal output of the fourth instar larvae was significantly lower than that of the RI and CK groups (P<0.05), and the trypsin activity of the fourth instar larvae was significantly higher than that of the other three groups (P<0.05). The body length and food availability of the fourth instar larvae did not significantly differ from those of the RI group, and the α-amylase activity of the fourth instar larvae did not significantly differ from those of the FM and RI groups. The body length, body weight, food intake, fecal output, food utilization, α-amylase activity, cellulase activity, GSTs activity, CarE activity, and MFO activity of the fifth instar larvae were significantly lower than those in the FM, RI and CK groups (P<0.05). The food consumption rate of the fifth instar larvae was significantly lower than that in the FM and RI groups, and the AchE activity of the fifth instar larvae was significantly lower than that in the FM group (P<0.05). The trypsin activity of the fifth instar larvae was significantly higher than that of the other three groups (P <0.05).【Conclusion】 The mixed inoculation of RI and FM could induce the chemical defense performance of P. pseudo-cathayana × P. deltoides leaves in the aspects of secondary metabolites, defense enzymes, and protease inhibitors. The anti-insect performance of combined RI and FM inoculation was better than that of single RI and FM inoculation and no AM fungi inoculation and had certain inhibitory effect on the larva of H. cunea. In practical application, combined RI and FM inoculation can be prioritized.

导出引用

方静, 张书曼, 严善春, 等. 两种丛枝菌根真菌复合接种对青山杨叶片抗美国白蛾的影响[J]. 南京林业大学学报（自然科学版）. 2024, 48(2): 144-154 https://doi.org/10.12302/j.issn.1000-2006.202209054

FANG Jing, ZHANG Shuman, YAN Shanchun, et al. Effects of the compound inoculation of two arbuscular mycorrhizal(AM) fungi on the resistance of Populus pseudo-cathayana × P. deltoides leaves to Hyphantria cunea[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2024, 48(2): 144-154 https://doi.org/10.12302/j.issn.1000-2006.202209054

中图分类号： S718.8;S763

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