牡丹野生种根际土壤细菌群落特征分析

doi:10.12302/j.issn.1000-2006.202210031

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南京林业大学学报（自然科学版） ›› 2023, Vol. 47 ›› Issue (3): 45-55.doi: 10.12302/j.issn.1000-2006.202210031

所属专题：牡丹培育与应用研究

• 专题报道：牡丹培育与应用研究（执行主编李维林张金池） • 上一篇下一篇

牡丹野生种根际土壤细菌群落特征分析

郭丽丽¹(), 张晨洁¹, 王菲², 沈佳佳¹, 张凯月¹, 何丽霞³, 郭琪¹, 侯小改¹^,^*()

1.河南科技大学农学院/牡丹学院,河南洛阳 471023
2.河南科技学院资源与环境学院,河南新乡 453003
3.甘肃省农业技术推广总站,甘肃兰州 730020

收稿日期:2022-10-24 修回日期:2022-12-04 出版日期:2023-05-30 发布日期:2023-05-25
通讯作者: 侯小改
基金资助:
河南省优秀青年基金项目(202300410119);国家自然科学基金河南省联合基金重点项目(U1804233);河南省中原学者项目(212101510003);河南省高校科技创新人才计划(22HASTIT036)

Analysis of bacterial community characteristics in the rhizosphere soil of wild tree peony

GUO Lili¹(), ZHANG Chenjie¹, WANG Fei², SHEN Jiajia¹, ZHANG Kaiyue¹, HE Lixia³, GUO Qi¹, HOU Xiaogai¹^,^*()

1. College of Agriculture/Tree Peony, Henan University of Science and Technology, Luoyang 471023, China
2. School of Resource and Environment, Henan Institute of Science and Technology, Xinxiang 453003, China
3. Gansu Provincial Agricultural Technology Promotion Station, Lanzhou 730020, China

Received:2022-10-24 Revised:2022-12-04 Online:2023-05-30 Published:2023-05-25
Contact: HOU Xiaogai

摘要/Abstract

摘要：

【目的】植物根际微生物群落由土壤环境和根系代谢活动共同作用产生,在植物生长发育过程中发挥重要功能。解析牡丹野生种在引种地根际土壤细菌群落特征,对有效利用微生物资源和保护野生植物种质资源具有重要的理论意义,为改良牡丹野生种的土壤环境、实现优质种质资源广谱性种植奠定基础。【方法】应用MiSeq高通量测序技术对大花黄牡丹(Paeonia ludlowii)、狭叶牡丹(P. potaninii)、紫牡丹(P. delavayi)、黄牡丹(P. lutea)、紫斑牡丹(P. rockii)、杨山牡丹(P. ostii)、四川牡丹(P. decomposita)、稷山牡丹(P. jishanensis)和卵叶牡丹(P. qiui)9个牡丹野生种根际土壤样品进行16S rRNA基因测序,并分析其与理化指标的相关性。【结果】高通量测序共获得606 536条序列和99个OTU聚类,隶属于24门、84纲、154目、280科和603属。Alpha多样性分析表明杨山牡丹根际微生物群落包含的物种数目最高,Beta分析发现四川牡丹、紫斑牡丹、黄牡丹、杨山牡丹、紫牡丹和大花黄牡丹根际土壤中细菌群落结构更为相似。细菌群落组成分析结果表明:根际土壤样本的核心优势细菌群落主要由变形菌门(Proteobatteria)、酸杆菌门(Acidobacteria)、放线菌门(Actinobacteria)和绿弯菌门(Chloroflexi)组成。酸杆菌门、绿弯菌门、硝化螺旋菌门(Nitrospirae)、芽单胞菌门(Gemmatimonadetes)、亚硝化螺菌属(Nitrosospira)和假单胞菌属(Pseudomonas)等有益菌种在不同野生种中存在显著差异。根际细菌群落功能预测多集中在代谢、遗传信息处理和环境信息处理方面。牡丹野生种根际细菌群落受土壤有机质和速效钾含量影响较大。【结论】不同野生种根际土壤细菌群落结构不同,细菌群落的形成与牡丹种类存在显著相关性,有益菌门/属的富集,对植株生长具有促进作用。今后可有效利用根际微生物资源以改良牡丹野生种的土壤环境,实现优质种质资源广谱性种植,为野生种质资源的保护奠定基础。

关键词: 牡丹, 野生种, 土壤性质, 根际细菌, 群落结构, 群落组成, 相关性分析

Abstract:

【Objective】 The microbial community in the rhizosphere is produced by the interactions of the soil environment and root metabolic activities, and plays an important role in plant growth and development. Analysis of characteristics of bacterial community in the rhizosphere soil of wild tree peony species possesses important theoretical significance for the effective use of microbial resources to protect wild plant germplasm resources and lays a foundation for improving the soil environment of wild tree peony species and realizing the broad-spectrum planting of high-quality germplasm resources. 【Method】 In the present study, MiSeq high-throughput sequencing technology with 16S rRNA genes was employed to investigate bacterial community characteristics in the rhizosphere soil of nine wild tree peony species (Paeonia ludlowii, P. potaninii, P. delavayi, P. lutea, P. rockii, P. ostii, P. decomposita, P. jishanensis and P. qiui) as well as their relevance with physicochemical properties. 【Result】A total of 606 536 sequences and 99 OTU clusters were obtained by high-throughput sequencing, which belonged to 24 phyla, 84 classes, 154 orders, 280 families and 603 genera. Alpha diversity analysis revealed that the rhizosphere microbial community of P. ostii contained the highest number of species. Beta analysis revealed that the bacterial community structures in the rhizosphere soils of P. decomposita, P. rockii, P. lutea, P. ostii, P. delavayi and P. ludlowii were similar. Analysis of bacterial community compositions showed that the core and dominant bacteria in the rhizosphere soil were Proteobacteria, Acidobacteria, Actinobacteria and Chloroflexi. Significant differences were found in beneficial bacteria among different wild tree species, such as Acidobacteria, Chloroflexi, Nitrospirae, Gemmatimonadetes, Nitrosospira and Pseudomonas. Predicted functions of the rhizosphere bacterial community have mostly focused on metabolism, genetic information processing, and environmental information processing. The bacterial community in the rhizosphere of wild peony trees is significantly affected by soil organic matter and available potassium. 【Conclusion】These results indicated that the bacterial community structure in the rhizosphere soil of different wild tree peony species was different, and the formation of a bacterial community was closely related to the varieties of tree peony. The enrichment in beneficial bacteria (genera) promoted plant growth. In the future, rhizospheric microbial resources could be effectively utilized to improve the soil environment of wild tree peonies, to realize the broad-spectrum planting of high-quality germplasm resources, and to lay the foundation for the protection of wild planting resources.

Key words: Paeonia × suffruticosa, wild tree peony, properties of rhizospheric soil, rhizospheric bacteria, community structure, community composition, correlation analysis

中图分类号:

S685.11
Q938

郭丽丽,张晨洁,王菲,等. 牡丹野生种根际土壤细菌群落特征分析[J]. 南京林业大学学报（自然科学版）, 2023, 47(3): 45-55.

GUO Lili, ZHANG Chenjie, WANG Fei, SHEN Jiajia, ZHANG Kaiyue, HE Lixia, GUO Qi, HOU Xiaogai. Analysis of bacterial community characteristics in the rhizosphere soil of wild tree peony[J].Journal of Nanjing Forestry University (Natural Science Edition), 2023, 47(3): 45-55.DOI: 10.12302/j.issn.1000-2006.202210031.

图/表 9

表1

图1

表2

图2

表3

根际土壤细菌在门水平的相对丰度"

种 species	变形菌门 Proteobacteria	酸杆菌门 Acidobacteria	放线菌门 Actinobacteria	绿弯菌门 Chloroflexi	细菌未分类 bacteria_ unclassified	芽单孢菌门 Gemmati- monadetes	拟杆菌门 Bacteroidetes	厚壁菌门 Firmicutes	硝化螺旋菌门 Nitrospirae	蓝菌门 Cyanobacteria	疣微菌门 Verrucomicrobia
四川牡丹 P. decomposita	33.89±0.60 a	35.61±1.42 a	11.27±0.99 b	3.52±0.04 c	4.59±0.15 a	4.36±0.28 a	2.19±0.24 ab	0.78±0.04 b	1.76±0.15 abc	0.03±0.01 a	0.54±0.01 b
紫斑牡丹 P. rockii	32.00±1.64 a	34.84±1.41 ab	9.07±0.46 b	4.10±0.41 bc	6.19±0.92 a	3.34±0.58 abc	3.05±0.90 ab	1.08±0.09 b	2.28±0.11 ab	0.06±0.05 a	1.72±0.31 a
黄牡丹 P. lutea	34.20±7.16 a	24.76±3.45 abc	16.64±1.09 b	3.12±0.28 c	5.78±1.90 a	2.94±0.44 abc	5.12±1.39 ab	0.83±0.24 b	1.28±0.19 bcd	2.19±1.94 a	0.91±0.26 ab
卵叶牡丹 P. qiui	22.09±7.56 ab	22.24±6.00 bc	27.12±9.94 ab	8.92±3.77 bc	7.96±2.10 a	2.77±1.07 abc	1.37±0.66 b	0.73±0.27 b	1.03±0.34 cde	1.95±1.89 a	1.19±0.35 ab
杨山牡丹 P. ostii	33.11±1.72 a	28.38±2.61 ab	14.04±2.94 b	4.37±1.02 bc	5.47±0.50 a	4.71±0.58 a	2.74±1.10 ab	1.43±0.08 b	1.63±0.13 abc	1.23±1.21 a	0.93±0.13 ab
狭叶牡丹 P. potanini	32.35±0.72 a	25.73±1.34 ab	17.79±4.65 b	4.12±0.3 bc	4.73±0.45 a	4.54±1.07 a	3.98±1.02 ab	1.03±0.06 b	1.49±0.06 bcd	1.04±0.63 a	1.21±0.06 ab
紫牡丹 P. delavayi	9.35±3.32 b	12.59±3.27 c	42.13±8.63 a	17.44±5.43 a	6.40±1.56 a	0.53±0.20 c	1.08±0.66 b	0.58±0.06 b	0.28±0.10 e	6.42±6.30 a	0.72±0.24 ab
稷山牡丹 P. jishanensis	13.53±8.46 b	12.80±7.54 c	37.77±11.87 a	12.83±4.50 ab	5.67±1.86 a	0.78±0.69 bc	1.06±0.91 b	12.83±9.64 a	0.59±0.55 de	0.05±0.04 a	0.38±0.23 b
大花黄牡丹 P. ludlowii	33.79±1.01 a	30.85±1.45 ab	9.30±0.54 b	3.89±0.15 bc	5.70±0.39 a	3.58±0.49 ab	5.49±0.18 a	0.96±0.12 b	2.58±0.10 a	0.16±0.08 a	1.21±0.15 ab

表3

图3

图4

图5

图6

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种 species	pH	有机质含量/ (g·kg^-1) organic matter content	全氮含量/ (g·kg^-1) total nitrogen content	速效磷含量/ (mg·kg^-1) available phosphorus content	速效钾含量/ (mg·kg^-1) available potassium content
四川牡丹P. decomposita	8.27±0.05 bc	10.04±0.61 a	0.52±0.06 a	4.33±0.19 e	115.09±4.05 cd
紫斑牡丹P. rockii	8.22±0.00 bc	9.78±2.35 a	0.29±0.01 c	5.28±0.48 e	121.20±1.81 c
黄牡丹P. lutea	8.16±0.02 c	6.08±0.98 b	0.49±0.06 a	17.29±0.44 a	149.12±4.95 b
卵叶牡丹P. qiui	8.16±0.03 c	7.07±0.60 ab	0.42±0.01 abc	13.10±0.29 b	88.73±5.29 e
杨山牡丹P. ostii	8.44±0.05 a	7.48±0.78 ab	0.40±0.05 abc	12.51±0.25 b	95.18±1.77 de
狭叶牡丹P. potanini	8.37±0.05 ab	4.67±0.14 b	0.29±0.03 c	6.32±0.16 d	75.22±6.39 e
紫牡丹P. delavayi	8.16±0.05 c	6.83±1.02 ab	0.32±0.04 bc	2.36±0.18 f	148.64±14.85 b
稷山牡丹P. jishanensis	8.29±0.04 bc	4.87±0.67 b	0.45±0.05 ab	2.56±0.19 f	120.72±5.27 c
大花黄牡丹P. ludlowii	8.21±0.04 bc	6.98±0.18 ab	0.38±0.03 abc	8.35±0.62 c	204.58±9.17 a

种 species	Chao1丰富度估计量 Chao1 richness estimator	物种丰富度指数 species richness index	香农多样性指数 Shannon diversity index	辛普森多样性指数 Simpson diversity index	测序深度指数 sequencing depth index
四川牡丹P. decomposita	3 413.24±43.53 a	2 105.67±68.39 ab	9.89±0.08 a	1.00±0.00 a	0.88±0.00 bc
紫斑牡丹P. rockii	2 803.58±609.36 ab	1 883.33±358.65 ab	9.82±0.35 a	1.00±0.00 a	0.90±0.03 bc
黄牡丹P. lutea	2 566.72±835.08 ab	1 627.33±443.25 ab	9.21±0.52 a	0.99±0.00 a	0.91±0.04 bc
卵叶牡丹P. qiui	1 860.23±527.97 bc	1 255.67±370.46 bc	8.24±1.03 ab	0.98±0.01 ab	0.94±0.02 ab
杨山牡丹P. ostii	3 627.01±524.86 a	2 208.00±204.96 a	9.90±0.31 a	1.00±0.00 a	0.86±0.02 c
狭叶牡丹P. potanini	3 344.83±363.80 ab	2 190.33±136.73 a	9.90±0.35 a	1.00±0.00 a	0.88±0.01b c
紫牡丹P. delavayi	596.59±172.05 c	505.00±140.52 c	5.82±0.69 c	0.90±0.04 c	0.98±0.01 a
稷山牡丹P. jishanensis	834.93±298.09 c	657.67±250.40 c	6.45±1.37 bc	0.93±0.04 bc	0.98±0.01 a
大花黄牡丹P. ludlowii	2 595.47±163.57 ab	1 845.00±55.30 ab	9.87±0.03 a	1.00±0.00 a	0.91±0.01 bc

牡丹野生种根际土壤细菌群落特征分析

Analysis of bacterial community characteristics in the rhizosphere soil of wild tree peony

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