长期氮磷添加对亚热带森林土壤有机碳储量及其组分的影响

doi:10.12302/j.issn.1000-2006.202301001

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南京林业大学学报（自然科学版） ›› 2024, Vol. 48 ›› Issue (5): 139-146.doi: 10.12302/j.issn.1000-2006.202301001

长期氮磷添加对亚热带森林土壤有机碳储量及其组分的影响

陈蕾如¹(), 温正宇¹^,², 徐小牛¹^,^*(), 尹若勇¹, 高雨¹

1.安徽农业大学林学与园林学院, 安徽合肥 230036
2.安徽科技学院, 安徽滁州 233100

收稿日期:2023-01-01 修回日期:2023-07-25 出版日期:2024-09-30 发布日期:2024-10-03
通讯作者: * 徐小牛(xnxu2007@ahau.edu.cn),教授。
作者简介:
陈蕾如(chenlr199605@163.com)。
基金资助:
国家自然科学基金项目(31770672)

Effects of long-term nitrogen and phosphorus additions on soil organic carbon storage and its components in a subtropical forest

CHEN Leiru¹(), WEN Zhengyu¹^,², XU Xiaoniu¹^,^*(), YIN Ruoyong¹, GAO Yu¹

1. College of Forestry and Landscape Architecture, Anhui Agricultural University, Hefei 230036, China
2. Anhui Science and Technology University, Chuzhou 233100, China

Received:2023-01-01 Revised:2023-07-25 Online:2024-09-30 Published:2024-10-03

1. 长期氮磷添加对亚热带森林土壤有机碳储量及其组分的影响.zip(1702KB)

摘要/Abstract

摘要：

【目的】长期高氮(N)沉降对具有磷(P)限制的亚热带森林土壤有机碳(SOC)及其储量具有影响,探究长期N添加下活性SOC、土壤有机氮(SON)组分对有机碳储量的影响,为亚热带森林土壤的碳(C)固存能力以及亚热带森林生态系统的可持续经营提供依据。【方法】在安徽省祁门县查湾自然保护区的常绿阔叶林内,选取中坡和坡顶两个区位,各设置3种不同的N、P添加处理,即对照(CK,每年N、P添加量为0 kg/hm²)、N添加(N,每年N添加量为100 kg/hm²)、N+P添加(N+P,每年N添加量为100 kg/hm²、P添加量为50 kg/hm²),各处理设置3个重复样地,大小为30 m×15 m,共18块样地。自2011年5月开始,进行为期10年的N及N+P添加野外模拟试验。于2020年10月,采集不同处理样地内[0,40)cm土层的土壤样品,测定SOC含量及储量、活性SOC组分、SON组分以及其他土壤基本理化性质。将采集的土样平均分成两份,一部分土样自然风干压碎后过孔径0.25 mm筛,用于测定土壤SOC及TN含量。另一部分鲜土样经不同处理后,用于测定SOC、SON组分及其他理化性质。【结果】与对照相比,连续10年N及N+P添加并未显著改变SOC储量,但显著降低土壤微生物生物量碳、氮。相关性分析结果表明,土壤有机碳储量与大多数土壤理化性质密切相关,除溶解性有机碳外,与其余有机碳氮组分均极显著正相关。另外,有机碳储量与pH、土壤容重呈显著负相关。线性回归分析结果显示,颗粒有机碳、氮对有机碳储量的解释率分别为75.4%、71.7%,微生物生物量碳、氮对有机碳储量的解释率分别为26.0%、49.3%;而土壤可溶性有机碳与有机碳储量无显著相关关系,可溶性氮对有机碳储量的解释率仅为11.4%。【结论】长期添加N及N+P后,土壤微生物生物量碳氮含量显著下降,并可能不利于土壤有机碳存量,而颗粒有机碳氮是对土壤有机碳储量影响最大的组分。

关键词: 氮磷添加, 土壤有机碳储量, 有机碳组分, 有机氮组分, 亚热带森林

Abstract:

【Objective】The aim of the present study is to simulate the effects of long-term nitrogen (N) deposition on soil organic carbon (SOC) and organic carbon storage in subtropical forests with phosphorus (P) limitation to investigate the effects of active SOC and soil organic nitrogen (SON) components on organic carbon storage under long-term N addition, as well as to provide the basis for soil carbon (C) sequestration capacity and sustainable management of subtropical forest ecosystems.【Method】In an evergreen broad-leaved forest of Zhawan Nature Reserve, Qimen County, Anhui Province, two sites were selected, the middle slope and flat ridge, for the present study. Three different N and P addition treatments were designed at each site as follows: control (CK, N and P addition of 0 kg/hm² per year), N addition (N, N addition of 100 kg/hm² per year), and N+P addition (N+P, N addition of 100 kg/hm² and P addition of 50 kg/hm² per year). There were three replicate plots of 30 m × 15 m for each treatment, with a total of 18 sampling plots. In October 2020, soil samples were collected from the 0-40 cm layer in each plot to determine SOC content, SOC storage, active SOC fraction, SON fraction, and other basic physicochemical properties. The collected soil samples were divided into two parts. One part of the soil sample was naturally air-dried, crushed, and passed through a 0.25 mm sieve for the determination of soil SOC and total nitrogen (TN) content. The other part of the soil sample was treated differently to determine soil SOC, SON fraction, and other physicochemical properties to investigate the effect of long-term simulated N deposition on the organic carbon storage capacity of subtropical forest soils, as well as the effects of SOC and SON fractions on organic carbon storage. 【Result】Compared to the CK treatment, N and P additions did not significantly change the SOC content and storage, but it significantly reduced the soil microbial biomass carbon (MBC) and microbial biomass nitrogen (MBN) contents. Correlation analysis showed that SOC storage was significantly and positively correlated with most soil physical and chemical properties but significantly and negatively correlated with pH and bulk density. Linear regression analysis demonstrated that particulate organic carbon (POC) and particulate organic nitrogen (PON) explained 75.4% and 71.7% of the effects on SOC storage, respectively, while MBC and MBN explained 26.0% and 49.3% of the effects on SOC storage, respectively. There was no significant relationship between dissolved organic carbon (DOC) and SOC storage, and DOC explained 11.4% of the effects on SOC storage.【Conclusion】Long-term N and N+P additions significantly reduce the C and N contents of soil microbial biomass and may be detrimental to SOC stocks, with POC and PON having the greatest impact on SOC storage.

Key words: nitrogen and phosphorus addition, soil organic carbon storage, soil organic carbon component, soil organic nitrogen component, subtropical forest

中图分类号:

S718

陈蕾如,温正宇,徐小牛,等. 长期氮磷添加对亚热带森林土壤有机碳储量及其组分的影响[J]. 南京林业大学学报（自然科学版）, 2024, 48(5): 139-146.

CHEN Leiru, WEN Zhengyu, XU Xiaoniu, YIN Ruoyong, GAO Yu. Effects of long-term nitrogen and phosphorus additions on soil organic carbon storage and its components in a subtropical forest[J].Journal of Nanjing Forestry University (Natural Science Edition), 2024, 48(5): 139-146.DOI: 10.12302/j.issn.1000-2006.202301001.

图/表 8

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参考文献 34

[1]	SMITH P. Soil carbon sequestration and biochar as negative emission technologies[J]. Glob Chang Biol, 2016, 22(3):1315-1324.DOI: 10.1111/gcb.13178.
[2]	LI W B, GAN X L, JIANG Y, et al. Nitrogen effects on grassland biomass production and biodiversity are stronger than those of phosphorus[J]. Environ Pollut, 2022, 309:119720.DOI: 10.1016/j.envpol.2022.119720.
[3]	FINZI A C, COLE J J, DONEY S C, et al. Research frontiers in the analysis of coupled biogeochemical cycles[J]. Front Ecol Environ, 2011, 9(1):74-80.DOI: 10.1890/100137.
[4]	LIU H F, ZHANG J Y, AI Z M, et al. 16-year fertilization changes the dynamics of soil oxidizable organic carbon fractions and the stability of soil organic carbon in soybean-corn agroecosystem[J]. Agric Ecosyst Environ, 2018, 265:320-330.DOI: 10.1016/j.agee.2018.06.032.
[5]	郭洁芸, 王雅歆, 李建龙. 氮添加对中国陆地生态系统植物-土壤碳动态的影响[J]. 生态学报, 2022, 42(12):4823-4833.
	GUO J Y, WANG Y X, LI J L. Effects of nitrogen addition on plant-soil carbon dynamics in terrestrial ecosystems of China[J]. Acta Ecol Sin, 2022, 42(12):4823-4833.DOI: 10.5846/stxb202108192313.
[6]	王朋朋, 王丹, 王昊, 等. 长期氮、磷添加对青藏高原2种高寒草甸植物光合特性的影响[J]. 江苏农业科学, 2019, 47(13):325-329.
	WANG P P, WANG D, WANG H, et al. Effects of long-term nitrogen and phosphorus addition on photosynthetic characteristics of two alpine meadow plants[J]. Jiangsu Agric Sci, 2019, 47(13):325-329.DOI: 10.15889/j.issn.1002-1302.2019.13.076.
[7]	周纪东, 史荣久, 赵峰, 等. 施氮频率和强度对内蒙古温带草原土壤pH及碳、氮、磷含量的影响[J]. 应用生态学报, 2016, 27(8):2467-2476.
	ZHOU J D, SHI R J, ZHAO F, et al. Effects of the frequency and intensity of nitrogen addition on soil pH,the contents of carbon,nitrogen and phosphorus in temperate steppe in Inner Mongolia,China[J]. Chin J Appl Ecol, 2016, 27(8):2467-2476.DOI: 10.13287/j.1001-9332.201608.037.
[8]	江晶, 武均, 张仁陟, 等. 碳氮添加对雨养农田土壤全氮、有机碳及其组分的影响[J]. 水土保持学报, 2019, 33(3):215-220,227.
	JIANG J, WU J, ZHANG R Z, et al. Effects of carbon and nitrogen addition on total nitrogen,organic carbon and their components in rain-fed farmland[J]. J Soil Water Conserv, 2019, 33(3):215-220,227.DOI: 10.13870/j.cnki.stbcxb.2019.03.032.
[9]	HUANG X M, TERRER C, DIJKSTRA F A, et al. New soil carbon sequestration with nitrogen enrichment:a meta-analysis[J]. Plant Soil, 2020, 454(1):299-310.DOI: 10.1007/s11104-020-04617-x.
[10]	习丹, 翁浩东, 胡亚林, 等. 林冠氮添加和林下植被去除对杉木林土壤有机碳组分的影响[J]. 生态学报, 2021, 41(21):8525-8534.
	XI D, WENG H D, HU Y L, et al. Effects of canopy nitrogen addition and understory removal on soil organic carbon fractions in a Chinese fir plantation[J]. Acta Ecol Sin, 2021, 41(21):8525-8534.DOI: 10.5846/stxb202008162130.
[11]	胡海清, 罗斯生, 罗碧珍, 等. 林火干扰对森林生态系统土壤有机碳的影响研究进展[J]. 生态学报, 2020, 40(6):1839-1850.
	HU H Q, LUO S S, LUO B Z, et al. Effects of forest fire disturbance on soil organic carbon in forest ecosystems:a review[J]. Acta Ecol Sin, 2020, 40(6):1839-1850.DOI: 10.5846/stxb201903040401.
[12]	ZHOU Z H, WANG C K. Reviews and syntheses:soil resources and climate jointly drive variations in microbial biomass carbon and nitrogen in China’s forest ecosystems[J]. Biogeosciences, 2015, 12(22):6751-6760.
[13]	曹佳锐, 龚可杨, 别宇静, 等. 水土保持林恢复土壤可溶性碳氮组分动态与三维荧光特征分析[J]. 生态学报, 2021, 41(19):7679-7688.
	CAO J R, GONG K Y, BIE Y J, et al. Analysis of dynamics of soil dissolved carbon and nitrogen fractions and its three-dimensional fluorescence characteristics during restoration of soil and water conservation forests[J]. Acta Ecol Sin, 2021, 41(19):7679-7688.DOI: 10.5846/stxb202006231635.
[14]	TOENSHOFF C, JOERGENSEN R G, STUELPNAGEL R, et al. Dynamics of soil organic carbon fractions one year after the re-conversion of poplar and willow plantations to arable use and perennial grassland[J]. Agric Ecosyst Environ, 2013, 174:21-27.DOI: 10.1016/j.agee.2013.04.014.
[15]	NIU S L, CLASSEN A T, DUKES J S, et al. Global patterns and substrate-based mechanisms of the terrestrial nitrogen cycle[J]. Ecol Lett, 2016, 19(6):697-709.DOI: 10.1111/ele.12591.
[16]	赵丹丹, 王俊, 付鑫. 长期定位施肥对旱作农田土壤全氮及其组分的影响[J]. 水土保持学报, 2016, 30(4):303-307.
	ZHAO D D, WANG J, FU X. Effect of long-term fertilization on soil total nitrogen and its fractions in dryland farming system[J]. J Soil Water Conserv, 2016, 30(4):303-307.DOI: 10.13870/j.cnki.stbcxb.2016.04.050.
[17]	林伟, 马红亮, 裴广廷, 等. 氮添加对亚热带森林土壤有机碳氮组分的影响[J]. 环境科学研究, 2016, 29(1):67-76.
	LIN W, MA H L, PEI G T, et al. Effects of nitrogen addition on soil carbon and nitrogen pools in mid-subtropical forest[J]. Res Environ Sci, 2016, 29(1):67-76.DOI: 10.13198/j.issn.1001-6929.2016.01.09.
[18]	DAI Y, WANG H, CHEN M, et al. Response of soil bacterial communities to nitrogen and phosphorus additions in an age-sequence of subtropical forests[J]. IForest, 2021, 14(1):71-79.DOI: 10.3832/ifor3655-013.
[19]	刘雅洁, 王亮, 樊伟, 等. 海拔对杉木人工林土壤活性有机碳组分的影响[J]. 西北农林科技大学学报(自然科学版), 2021, 49(8):59-69.
	LIU Y J, WANG L, FAN W, et al. Effects of altitude on soil active organic carbon components in Cunninghamia lanceolata plantations[J]. J Northwest A F Univ (Nat Sci Ed), 2021, 49(8):59-69.DOI: 10.13207/j.cnki.jnwafu.2021.08.008.
[20]	许慧, THANG B, 范洪旺. 城市和郊区麻栎林土壤有机碳组分及管理指数变化特征[J]. 江苏农业学报, 2022, 38(2):369-376.
	XU H, THANG B, FAN H W, et al. Variation characteristics of soil organic carbon composition and management index of Ouercus acutissima forests in urban and suburban areas[J]. Jiangsu J Agri Sci, 2022, 38(2):369-376. DOI: 10.3969/i.issn.1000-4440.2022.02.010.
[21]	邓小文, 韩士杰. 氮沉降对森林生态系统土壤碳库的影响[J]. 生态学杂志, 2007, 26(10):1622-1627.
	DENG X W, HAN S J. Impact of nitrogen deposition on forest soil carbon pool[J]. Chin J Ecol, 2007, 26(10):1622-1627.
[22]	李素新, 覃志杰, 刘泰瑞, 等. 模拟氮沉降对华北落叶松人工林土壤微生物碳和微生物氮的动态影响[J]. 水土保持学报, 2020, 34(1):268-274.
	LI S X, QIN Z J, LIU T R, et al. Effects of simulated nitrogen deposition on soil microbial carbon and nitrogen dynamics of Larix principis-rupprechtii plantation[J]. J Soil Water Conserv, 2020, 34(1):268-274.DOI: 10.13870/j.cnki.stbcxb.2020.01.039.
[23]	苏梓锐, 曾发旭, 郑成洋. 氮添加对亚热带常绿阔叶林土壤有机碳及土壤呼吸的影响[J]. 北京大学学报(自然科学版), 2022, 58(3):517-525.
	SU Z R, ZENG F X, ZHENG C Y. Effects of nitrogen addition on soil organic carbon and soil respiration in subtropical evergreen broad-leaved forest[J]. Acta Sci Nat Univ Pekin, 2022, 58(3):517-525.DOI: 10.13209/j.0479-8023.2022.017.
[24]	向元彬, 周世兴, 肖永翔, 等. 模拟氮沉降和降雨量改变对华西雨屏区常绿阔叶林土壤有机碳的影响[J]. 生态学报, 2017, 37(14):4686-4695.
	XIANG Y B, ZHOU S X, XIAO Y X, et al. Effects of simulated nitrogen deposition and precipitation changes on soil organic carbon in an evergreen broad-leaved forest that is part of the Rainy Area of Western China[J]. Acta Ecol Sin, 2017, 37(14):4686-4695.DOI: 10.5846/stxb201604250784.
[25]	CHEN X M, LIU J X, DENG Q, et al. Effects of elevated CO₂ and nitrogen addition on soil organic carbon fractions in a subtropical forest[J]. Plant Soil, 2012, 357(1):25-34.DOI: 10.1007/s11104-012-1145-3.
[26]	ZAK D R, HOLMES W E, BURTON A J, et al. Simulated atmospheric $NO_{3}^{-}$ deposition increases soil organic matter by slowing decomposition[J]. Ecol Appl, 2008, 18(8):2016-2027.DOI: 10.1890/07-1743.1.
[27]	祁瑜, MULDE J, 段雷, 等. 模拟氮沉降对克氏针茅草原土壤有机碳的短期影响[J]. 生态学报, 2015, 35(4):1104-1113.
	QI Y, MULDER J, DUAN L, et al. Short-term effects of simulating nitrogen deposition on soil organic carbon in a Stipa krylovii steppe[J]. Acta Ecol Sin, 2015, 35(4):1104-1113.DOI: 10.5846/stxb201304230779.
[28]	涂利华, 胡庭兴, 张健, 等. 模拟氮沉降对华西雨屏区慈竹林土壤活性有机碳库和根生物量的影响[J]. 生态学报, 2010, 30(9):2286-2294.
	TU L H, HU T X, ZHANG J, et al. Effects of simulated nitrogen deposition on soil active carbon pool and root biomass in Neosinocalamus affinis plantation,rainy area of West China[J]. Acta Ecol Sin, 2010, 30(9):2286-2294.
[29]	全智, 刘轩昂, 刘东. 土壤可溶性有机氮研究进展[J]. 应用生态学报, 2022, 33(1):277-288.
	QUAN Z, QUAN Z, LIU X A, LIU D. Research progress on soil soluble organic nitrogen[J]. Chin J Appl Ecol, 2022, 33(1):277-288.DOI: 10.13287/j.1001-9332.202201.010.
[30]	KHALID M, SOLEMAN N, JONES D L. Grassland plants affect dissolved organic carbon and nitrogen dynamics in soil[J]. Soil Biol Biochem, 2007, 39(1):378-381.DOI: 10.1016/j.soilbio.2006.07.007.
[31]	杜雪, 王海燕. 中国森林土壤有机碳活性组分及其影响因素[J]. 世界林业研究, 2022, 35(1):76-81.
	DU X, WANG H Y. Active components of forest soil organic carbon and its influencing factors in China[J]. World For Res, 2022, 35(1):76-81.DOI: 10.13348/j.cnki.sjlyyj.2021.0068.y.
[32]	CHEN J G, XIAO W, ZHENG C Y, et al. Nitrogen addition has contrasting effects on particulate and mineral-associated soil organic carbon in a subtropical forest[J]. Soil Biol Biochem, 2020, 142:107708.DOI: 10.1016/j.soilbio.2020.107708.
[33]	ROUSK J, BROOKES P C, BÅÅTH E. Contrasting soil pH effects on fungal and bacterial growth suggest functional redundancy in carbon mineralization[J]. Appl Environ Microbiol, 2009, 75(6):1589-1596.DOI: 10.1128/AEM.02775-08.
[34]	YE C L, CHEN D M, HALL S J, et al. Reconciling multiple impacts of nitrogen enrichment on soil carbon:plant,microbial and geochemical controls[J]. Ecol Lett, 2018, 21(8):1162-1173.DOI: 10.1111/ele.13083.

项目 item	df	土壤有机碳储量 SOC storage		土壤有机碳含量 SOC content		可溶性有机碳含量 DOC content		颗粒有机碳含量 POC content		微生物生物量碳含量 MBC content		可溶性有机氮含量 DON content		颗粒有机氮含量 PON content		微生物生物量氮含量 MBN content
项目 item	df	F	P	F	P	F	P	F	P	F	P	F	P	F	P	F	P
处理 treatment	2	1.33	0.27	0.66	0.52	1.57	0.22	0.59	0.56	15.55^**	<0.01	9.54^**	<0.01	0.35	0.71	8.89^**	<0.01
土层 layer	3	62.13^**	<0.01	107.64^**	<0.01	4.79^**	<0.01	55.50^**	<0.01	6.97^**	<0.01	14.07^**	<0.01	52.55^**	<0.01	12.73^**	<0.01
处理×土层 treatment×layer	6	0.12	0.99	0.19	0.98	0.14	0.99	0.04	1.00	0.56	0.76	1.47	0.20	0.11	1.00	0.12	0.99

长期氮磷添加对亚热带森林土壤有机碳储量及其组分的影响

Effects of long-term nitrogen and phosphorus additions on soil organic carbon storage and its components in a subtropical forest

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[2]	李建华, 夏虹露, 唐卫平, 黄晗. 亚热带典型森林类型可燃物载量分配特征[J]. 南京林业大学学报（自然科学版）, 2023, 47(5): 57-64.
[3]	赵晓雅, 关梦冉, 孙孟瑶, 王泽夫, 徐小牛. 氮磷添加对亚热带常绿阔叶林凋落物产量及其养分含量的影响[J]. 南京林业大学学报（自然科学版）, 2020, 44(6): 55-62.
[4]	曹林,朱兴洲,代劲松,许子乾,佘光辉. 基于机载小光斑LiDAR数据插值的亚热带森林丘陵地形的误差分析[J]. 南京林业大学学报（自然科学版）, 2014, 38(04): 7-13.