基于SSR标记的皱皮木瓜遗传多样性分析及品种分子身份证构建

李慧; 侯立娜; 王天琪; 毕宁宁; 李圣波; 刘忠华

doi:10.12302/j.issn.1000-2006.202301027

李慧, 侯立娜, 王天琪, 毕宁宁, 李圣波, 刘忠华

南京林业大学学报（自然科学版） ›› 2025, Vol. 49 ›› Issue (1) : 59-68.

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南京林业大学学报（自然科学版） ›› 2025, Vol. 49 ›› Issue (1) : 59-68. DOI: 10.12302/j.issn.1000-2006.202301027

研究论文

基于SSR标记的皱皮木瓜遗传多样性分析及品种分子身份证构建

李慧 ¹^,² ,
侯立娜 ¹^,² ,
王天琪 ² ,
毕宁宁 ² ,
李圣波 ³ ,
刘忠华 ¹^,²^,^*

作者信息 +

The genetic diversity analysis and molecular ID establishment of Chaenomeles speciosa based on SSR markers

LI Hui ¹^,² ,
HOU Lina ¹^,² ,
WANG Tianqi ² ,
BI Ningning ² ,
LI Shengbo ³ ,
LIU Zhonghua ¹^,²^,^*

Author information +

文章历史 +

摘要

【目的】皱皮木瓜(Chaenomeles speciosa)具有较高的观赏价值食用价值与药用价值,我国是皱皮木瓜的起源分布中心,研究皱皮木瓜种质资源遗传多样性,并构建品种分子身份证,以解决近年来因缺乏品种间统一的分类标准,同名异种、同种异名和品种间来源及演化不清等问题。【方法】以收集的168 份皱皮木瓜种质资源为材料,利用SSR标记结合毛细管电泳法对木瓜遗传多样性和群体内遗传分化程度进行分析,根据观测等位基因数(N_a)、Shannon’s信息指数(I)和多态性信息含量(PIC)的筛选能区分全部种质的引物组合,并基于字符串编码构建DNA分子身份证。【结果】26 对引物在168 个皱皮木瓜品种中共扩增出304 个等位基因,平均每个位点扩增出11.577 个;期望杂合度(H_e)、I和PIC的平均值分别为0.748、1.731和0.607。根据聚类分析的结果可将该群体分为2 大类,进一步分为6 个类群;种群结构分析将供试材料分为2 个亚类。从26 对引物中筛选出11 对核心引物构建了168 份皱皮木瓜种质资源的条形码和二维码身份证。【结论】所选扩增位点的变异程度高、鉴别力度大,在进行遗传多样性分析、核心引物筛选,以及指纹图谱构建中可优先选用。该研究为皱皮木瓜的良种鉴定、遗传资源管理及种质资源数据库的构建等提供了参考。

Abstract

【Objective】Chaenomeles speciosa has high ornamental, edible, and medicinal values. China is the origin and distribution center of C. speciosa. The genetic diversity of Chaenomeles speciosa germplasm resources was studied, and the molecular identity card of varieties was constructed to solve the problems of lack of unified classification criteria among varieties, homonymous and synoonymous, and unclear origin and evolution among varieties in recent years.【Method】A total of 168 C. speciosa varieties and SSR (simple sequence repeats) markers were combined with capillary electrophoresis to analyze the genetic diversity and the degree of genetic differentiation. The observed number of alleles (N_a), Shannon’s information index (I), and the polymorphism information content (PIC) were employed to screen primer combinations that can distinguish the entire germplasm and construct DNA molecular IDs based on string codes. 【Result】The results showed that 26 pairs of primers amplified 304 alleles in 168 C. speciosa varieties, with an average of 11.577 per locus. The average expected heterozygosity (H_e), I and PIC were determined as 0.748, 1.731 and 0.607, respectively. Based on the cluster analysis, the population could be divided into two groups, and further into six groups. Moreover, based on population structure analysis, the tested materials was divided into two subgroups. Four pairs of core primers were selected from 26 pairs of primers to construct barcode and two-dimensional code identification cards of C. speciosa varieties. 【Conclusion】The selected amplification loci have a high degree of variation and strong discrimination, and are thus suitable for applications in genetic diversity analysis, core primer screening, and fingerprint construction. The results can provide a reference for the variety identification, genetic resource management, and the construction of a germplasm resource database of C. speciosa.

导出引用

李慧, 侯立娜, 王天琪, 等. 基于SSR标记的皱皮木瓜遗传多样性分析及品种分子身份证构建[J]. 南京林业大学学报（自然科学版）. 2025, 49(1): 59-68 https://doi.org/10.12302/j.issn.1000-2006.202301027

LI Hui, HOU Lina, WANG Tianqi, et al. The genetic diversity analysis and molecular ID establishment of Chaenomeles speciosa based on SSR markers[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2025, 49(1): 59-68 https://doi.org/10.12302/j.issn.1000-2006.202301027

中图分类号： S793.9;S567.9

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