
氮添加下干旱对油茶根系氮吸收与分配的影响
姜孝曾, 祝燕, 周恒伟, 黄兴召, 傅龙龙, 万芳芳
南京林业大学学报(自然科学版) ›› 2025, Vol. 49 ›› Issue (1) : 95-102.
氮添加下干旱对油茶根系氮吸收与分配的影响
Effects of drought on nitrogen uptake and distribution in Camellia oleifera root under nitrogen addition
【目的】探讨油茶(Camellia oleifera)不同根序根系的生长与养分利用对干旱和施氮的响应,分析水和氮素对油茶不同径级根系生长、氮素吸收、分配和利用的影响,为更好地认识气候变暖下植物根系构型与养分策略的关系提供理论支撑。【方法】以2年生‘长林53’(‘Changlin 53’)油茶幼树为试材,利用盆栽试验和15N同位素示踪技术,采用双因素完全随机设计,设置2个干旱水平,即正常灌溉(土壤含水率75%±5%)和干旱(土壤含水率30%±5%);2个施氮水平,即不施氮和施15N标记的硝酸铵(2.88 g/株)。干旱处理75 d后取样测定油茶幼树不同径级根系的生物量、全氮含量(质量分数,下同)、来自肥料氮的百分率(Ndff)、氮素利用率。【结果】干旱胁迫后油茶1~3级,5级和6级根的生物量与其氮含量呈负相关关系,与4级根的则呈正相关关系。干旱显著影响了各径级根系15N含量和Ndff(P<0.05),抑制了各径级根系15N的富集,干旱胁迫下1~3级的Ndff最高。干旱对根系的氮素分配率起促进作用,尤其是5级根氮素增加了93.10%,但在不同程度上抑制了油茶低阶根系的氮素利用率。【结论】施氮促进干旱下根系生物量和氮素分配给粗根,但不利于细根生物量和氮素的积累;干旱显著影响了各级根系对肥料氮的吸收、利用和分配,促进肥料中的氮素向根系尤其5级以上根系分配,但不同程度地抑制了1~4级根系对15N的吸收和利用。
【Objective】This study investigates the growth response and nutrient utilization of the Camellia oleifera root system across different root orders under drought and nitrogen application. It aims to analyze how water and nitrogen affect root growth, nitrogen uptake, distribution and utilization, and to provide theoretical support for understanding the relationship between plant root architecture and nutrient strategies under climate warming. 【Method】 Two-year-old C. oleifera ‘Changlin 53’ trees were used. A pot experiment with 15N isotope tracing technology was conducted, setting two drought levels: normal irrigation (soil moisture content 75% ± 5%) and drought (soil moisture content 30%±5%). Two nitrogen application levels were used: no nitrogen and 15N-labeled ammonium nitrate (2.88 g/plant). After 75 days of drought treatment, the biomass, total nitrogen content, percentage of nitrogen from fertilizer (Ndff), and nitrogen use efficiency of roots of different diameter classes of C. oleifolia seedlings were measured. 【Result】 Under drought stress, there was a negative correlation between biomass and nitrogen content in grade 1-3, grade 5, and grade 6 roots, while a positive correlation was observed in grade 4 roots. Drought significantly affected 15N content and Ndff in all root diameter classes (P < 0.05). Drought inhibited 15N accumulation in all root diameter classes, with Ndff in grades 1-3 being most affected. Drought increased nitrogen distribution in roots, especially in grade 5 roots, which saw a 93.10% increase. However, the nitrogen use efficiency was inhibited to varying degrees across different root diameter classes. 【Conclusion】Nitrogen application increased root biomass and nitrogen allocation to coarse roots under drought but reduced fine root biomass and nitrogen accumulation. Drought significantly impacted the absorption, utilization, and distribution of fertilizer nitrogen in roots of all levels, enhancing nitrogen distribution, particularly in roots above grade 5, but inhibiting 15N absorption and utilization in roots of grades 1-4.
Camellia oleifera / nitrogenous addition / drought stress / root order / nitrogen fertilizer utilization
[1] |
|
[2] |
IPCC. Climate change 2022:mitigation of climate change[M]. Cambridge: Cambridge University Press, 2022.
|
[3] |
董斌, 洪文泓, 黄永芳, 等. 广西4个油茶品种苗期对干旱胁迫的生理响应[J]. 中南林业科技大学学报, 2018, 38(2):1-8.
|
[4] |
|
[5] |
|
[6] |
|
[7] |
|
[8] |
|
[9] |
田丽华. 氮对作物生长发育的影响及其施肥方法[J]. 河北农业科技, 2008(15):40.
|
[10] |
|
[11] |
|
[12] |
|
[13] |
|
[14] |
|
[15] |
黄小辉, 吴焦焦, 王玉书, 等. 不同供氮水平的核桃幼苗生长及叶绿素荧光特性[J]. 南京林业大学学报(自然科学版), 2022, 46(2):119-126.
|
[16] |
|
[17] |
|
[18] |
|
[19] |
|
[20] |
温伦敏. 江西省油茶林土壤肥力现状及养分优化管理建议[J]. 现代农业科技, 2016(9):197,200.
|
[21] |
杨婷婷, 王雪梅, 陈波浪, 等. 施氮对库尔勒香梨树体氮素吸收和积累的影响[J]. 中国土壤与肥料, 2016(4):103-107.
|
[22] |
|
[23] |
|
[24] |
|
[25] |
杨安, 李燕青, 李壮, 等. 氮磷钾肥不同滴灌撒施组合对富士苹果15N吸收分配及利用率的影响[J]. 果树学报, 2022, 39(4):564-573.
|
[26] |
黄海霞, 杨琦琦, 崔鹏, 等. 裸果木幼苗根系形态和生理特征对水分胁迫的响应[J]. 草业学报, 2021, 30(1):197-207.
|
[27] |
冰德叶. 氮添加与凋落物覆盖对油松和华山松幼苗生长的影响[D]. 杨凌: 西北农林科技大学, 2021.
|
[28] |
李敏, 赵熙州, 王好运, 等. 干旱胁迫及外生菌根菌对马尾松幼苗根系形态及分泌物的影响[J]. 林业科学, 2022, 58(7):63-72.
|
[29] |
|
[30] |
张国娟, 濮晓珍, 张鹏鹏, 等. 干旱区棉花秸秆还田和施肥对土壤氮素有效性及根系生物量的影响[J]. 中国农业科学, 2017, 50(13):2624-2634.
|
[31] |
|
[32] |
陶晨悦, 邵珊璐, 史文辉, 等. 氮沉降对干旱胁迫下毛竹实生苗生物量和保护酶活性的影响[J]. 林业科学, 2019, 55(9):31-40.
|
[33] |
曲秋玲, 王国梁, 刘国彬, 等. 施氮对白羊草细根形态和生长的影响[J]. 水土保持通报, 2012, 32(2):74-79.
|
[34] |
王瑞, 陈隆升, 王湘南, 等. 氮素形态对油茶苗木生长及生理指标的影响[J]. 南京林业大学学报(自然科学版), 2019, 43(4):26-32.
|
[35] |
|
[36] |
|
[37] |
陈丽楠, 韩晓日, 孙占祥, 等. 局部根区交替灌溉与氮素耦合对葡萄生长及15N-尿素利用的影响[J]. 核农学报, 2021, 35(2):447-453.
|
[38] |
|
[39] |
马晓东, 钟小莉, 桑钰. 干旱胁迫下胡杨实生幼苗氮素吸收分配与利用[J]. 生态学报, 2018, 38(20):7508-7519.
|
[40] |
李树斌, 周丽丽, 伍思攀, 等. 不同氮素形态对干旱胁迫杉木幼苗养分吸收及分配的影响[J]. 植物营养与肥料学报, 2020, 26(1):152-162.
|
/
〈 |
|
〉 |