青钱柳二倍体和四倍体叶特征比较研究

刘夏岚, 宋子琪, 胡凤荣, 尚旭岚

南京林业大学学报（自然科学版） ›› 2024, Vol. 48 ›› Issue (4) : 76-84.

PDF(2639 KB)
南京林业大学学报（自然科学版）
PDF(2639 KB)
南京林业大学学报（自然科学版） ›› 2024, Vol. 48 ›› Issue (4) : 76-84. DOI: 10.12302/j.issn.1000-2006.202305024
专题报道Ⅱ：乡村振兴视域下药用树种青钱柳培育研究（执行主编 李维林、方升佐）

青钱柳二倍体和四倍体叶特征比较研究

作者信息 +

A comparative study on leaf characters between diploid and tetraploid of Cyclocarya paliurus

Author information +
文章历史 +

摘要

【目的】比较青钱柳(Cyclocarya paliurus)二倍体和四倍体叶表型特征、解剖结构和气孔特征等方面的差异,旨在获得不同倍性青钱柳叶片特征的基础数据。【方法】以青钱柳二倍体和四倍体苗木为材料,对32项叶片特征指标进行测定,并进行独立样本t检验、相关性分析和主成分分析。【结果】除中部小叶的最大叶宽位置和锯齿宽度在倍性间无显著差异外,其余30项叶特征在倍性间均达到显著或极显著差异。其中,四倍体小叶数量极显著少于二倍体,而复叶面积和比叶质量极显著大于二倍体;四倍体顶端小叶和中部小叶的叶长、叶宽和叶面积极显著大于二倍体,而叶形指数极显著小于二倍体;四倍体锯齿密度极显著小于二倍体;四倍体叶片厚度、上表皮厚度、下表皮厚度、栅栏组织厚度和海绵组织厚度均极显著大于二倍体,而栅海比和紧密度极显著小于二倍体;四倍体气孔长度和气孔宽度极显著大于二倍体,而气孔密度极显著小于二倍体。相关性分析结果表明,青钱柳表型特征与解剖结构和气孔特征各指标间存在广泛而显著的相关关系。主成分分析提取了反映两种倍性青钱柳叶特征差异的指标,分别为小叶叶宽、小叶面积、叶形指数、海绵组织厚度、气孔长度、栅栏组织厚度和紧密度。【结论】青钱柳二倍体和四倍体的叶特征存在显著差异,小叶叶宽、小叶面积和叶形指数等叶表型特征可用于青钱柳二倍体和四倍体初筛。

Abstract

【Objective】To compare the differences in leaf phenotypes characters, anatomical structures and stomatal characteristics between diploid and tetraploid Cyclocarya paliurus, we obtained data on the leaf characteristics of C. paliurus with different ploidy.【Method】Diploid and tetraploid seedlings of C. paliurus were used as study materials, and 32 leaf traits were measured. Independent samples t-tests, a correlation analysis, and principal component analysis (PCA) were conducted.【Result】There were no significant differences between diploid and tetraploid seedlings for sawtooth width and the position of the maximum leaf width of the middle leaflet. For the other 30 leaf traits, there were significant or extremely significant differences between diploid and tetraploid seedlings. Among them, the number of leaflets of tetraploids was significantly less than that of diploids, while the area and specific leaf weight of compound leaves of tetraploids were significantly greater than those of diploids. The length, width and area of the terminal and middle leaflets of tetraploids were significantly greater than those of diploids, while the leaf shape index was significantly smaller than that of diploids. The sawtooth density of tetraploids was significantly smaller than that of diploids. The leaflet thickness, the upper epidermis thickness, lower epidermis thickness, palisade tissue thickness, and sponge tissue thickness of tetraploids were all significantly higher than those of diploids, while the ratio of palisade tissue to sponge tissue and cell tense ratio were significantly smaller than those of diploids. The stomatal length and width of tetraploids were significantly greater than those of diploids, while the stomatal density was significantly smaller than that of diploids. The correlation analysis results indicated that there were broad and significant correlations between the phenotypic characteristics, anatomical structure, and stomatal characteristics of C. paliurus. The indicators reflecting the differences of leaf characteristics between diploid and tetraploid C. paliurus were extracted by a PCA, including leaflet width, leaflet area, leaf shape index, sponge tissue thickness, stomatal length, palisade tissue thickness and cell tense ratio.【Conclusion】There were significant differences in leaf traits between diploid and tetraploid C. paliurus, and leaf phenotypic characteristics, such as leaflet width, leaflet area, and leaf shape index, can be used for the preliminary screening of diploid and tetraploid C. paliurus.

关键词

青钱柳 / 多倍体 / 表型特征 / 解剖结构 / 气孔特征

Key words

Cyclocarya paliurus / polyploid / phenotypic characters / anatomical structure / stomatal characteristics

引用本文

EndNote

Ris (Procite)

Bibtex

导出引用
刘夏岚, 宋子琪, 胡凤荣, 等. 青钱柳二倍体和四倍体叶特征比较研究[J]. 南京林业大学学报（自然科学版）. 2024, 48(4): 76-84 https://doi.org/10.12302/j.issn.1000-2006.202305024
LIU Xialan, SONG Ziqi, HU Fengrong, et al. A comparative study on leaf characters between diploid and tetraploid of Cyclocarya paliurus[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2024, 48(4): 76-84 https://doi.org/10.12302/j.issn.1000-2006.202305024
中图分类号： S722   

参考文献

列表( 原文顺序 | 文献年度倒序 | 文中引用次数倒序 ) 可视化分析
[1]
WOOD T E, TAKEBAYASHI N, BARKER M S, et al. The frequency of polyploid speciation in vascular plants[J]. Proc Natl Acad Sci USA, 2009, 106(33):13875-13879.DOI: 10.1073/pnas.0811575106.
本文引用 [1]
[2]
GUO W, YANG J, SUN X D, et al. Divergence in eco-physiological responses to drought mirrors the distinct distribution of Chamerion angustifolium cytotypes in the Himalaya-Hengduan mountains region[J]. Front Plant Sci, 2016, 7:1329.DOI: 10.3389/fpls.2016.01329.
本文引用 [1]
[3]
袁静, 周冰莹. 植物叶缘锯齿发育调控的研究进展[J]. 中国农学通报, 2021, 37(26):24-31.
YUAN J, ZHOU B Y. The regulation of leaf margin serration development in plants:a review[J]. Chin Agric Sci Bull, 2021, 37(26):24-31.
本文引用 [1]
[4]
晁亚琮, 武荣花, 蒋卉, 等. 同源多倍体化对植物侧生器官形态建成影响的研究进展[J]. 河南农业科学, 2021, 50(5):1-6.
CHAO Y C, WU R H, JIANG H, et al. Research progress on effect of autopolyploid on plant lateral organ morphogenesis[J]. J Henan Agric Sci, 2021, 50(5):1-6.DOI: 10.15933/j.cnki.1004-3268.2021.05.001.
本文引用 [1]
[5]
方升佐. 青钱柳产业发展历程及资源培育研究进展[J]. 南京林业大学学报(自然科学版), 2022, 46(6):115-126.
FANG S Z. A review on the development history and the resource silviculture of Cyclocarya paliurus industry[J]. J Nanjing For Univ (Nat Sci Ed), 2022, 46(6):115-126.DOI: 10.12302/j.issn.1000-2006.202206019.
本文引用 [1]
[6]
方升佐, 尚旭岚, 洑香香. 青钱柳种子生物学研究[M]. 北京: 中国林业出版社, 2017.
FANG S Z, SHANG X L, FU X X. Seed biology of Cyclocarya paliurus[M]. Beijing: China Forestry Publishing House, 2017.
本文引用 [1]
[7]
XIA X C, MAO D X, DAI H M, et al. Effect of Cyclocarya paliurus polysaccharides on streptozotocin-induced diabetic nephropathy in rats[J]. J Tradit Chin Med, 2020, 40(6):956-964.DOI: 10.19852/j.cnki.jtcm.2020.06.007.
本文引用 [1]
[8]
肖岩, 马博稷, 李冰涛, 等. 青钱柳醇提物中化学成分的UHPLC-Q-TOF/MS分析[J]. 中国实验方剂学杂志, 2022, 28(16):196-204.
XIAO Y, MA B J, LI B T, et al. Analysis of chemical constituents in ethanol extract of Cyclocarya paliurus dried leaves by UHPLC-Q-TOF/MS[J]. Chin J Exp Tradit Med Formulae, 2022, 28(16):196-204.DOI: 10.13422/j.cnki.syfjx.20212051.
本文引用 [1]
[9]
WANG Y R, CUI B S, HAN S W, et al. New dammarane triterpenoid saponins from the leaves of Cyclocarya paliurus[J]. J Asian Nat Prod Res, 2018, 20(11):1019-1027.DOI: 10.1080/10286020.2018.1457653.
本文引用 [1]
[10]
陈絮蒙, 王雅靖, 陈靓, 等. 青钱柳活性物质及其代谢调节作用的研究与应用进展[J]. 食品与发酵工业, 2023, 49(12):336-344.
CHEN X M, WANG Y J, CHEN L, et al. Research and application progress of active substances and their metabolic regulatory effects of Cyclocarya paliurus[J]. Food Ferment Ind, 2023, 49(12):336-344.DOI: 10.13995/j.cnki.11-1802/ts.035077.
本文引用 [2]
[11]
聂小华, 吴聪聪, 林胜利, 等. 青钱柳中活性物质及其功能特性研究进展[J]. 浙江工业大学学报, 2022, 50(2):222-227.
NIE X H, WU C C, LIN S L, et al. Advance on active compounds and functional properties of Cyclocarya paliurus (Batal.) Iljinskaja[J]. J Zhejiang Univ Technol, 2022, 50(2):222-227.DOI: 10.3969/j.issn.1006-4303.2022.02.017.
本文引用 [1]
[12]
田力, 徐骋炜, 尚旭岚, 等. 青钱柳药用优良单株评价与选择[J]. 南京林业大学学报(自然科学版), 2021, 45(1):21-28.
TIAN L, XU C W, SHANG X L, et al. Evaluation and selection on superior individuals for medicinal use of Cyclocarya paliurus[J]. J Nanjing For Univ (Nat Sci Ed), 2021, 45(1):21-28.DOI: 10.12302/j.issn.1000-2006.202002018.
本文引用 [1]
[13]
徐展宏, 朱莹, 金慧颖, 等. 不同叶色青钱柳叶片色素、多酚含量及光合特性的差异[J]. 南京林业大学学报(自然科学版), 2022, 46(2):103-110.
XU Z H, ZHU Y, JIN H Y, et al. Variations in the contents of leaf pigments and polyphenols and photosynthesis traits in Cyclocarya paliurus with different leaf colors[J]. J Nanjing For Univ (Nat Sci Ed), 2022, 46(2):103-110.DOI: 10.12302/j.issn.1000-2006.202105048.
本文引用 [1]
[14]
吴玲利, 韩航, 曾艳玲, 等. 青钱柳组织培养及快速繁殖[J]. 植物生理学报, 2019, 55(1):61-68.
WU L L, HAN H, ZENG Y L, et al. Tissue culture and rapid propagation of Cyclocarya paliurus[J]. Plant Physiol J, 2019, 55(1):61-68.DOI: 10.13592/j.cnki.ppj.2018.0360.
本文引用 [1]
[15]
QU Y Q, SHANG X L, ZENG Z Y, et al. Whole-genome duplication reshaped adaptive evolution in a relict plant species,Cyclocarya paliurus[J]. Genomics Proteomics Bioinformatics, 2023:S1672 -S0229(23)00033-5.DOI: 10.1016/j.gpb.2023.02.001.
本文引用 [1]
[16]
MAO X, FU X X, HUANG P, et al. Heterodichogamy,pollen viability,and seed set in a population of polyploidy Cyclocarya paliurus (Batal) Iljinskaja (Juglandaceae)[J]. Forests, 2019, 10(4):347.DOI: 10.3390/f10040347.
本文引用 [1]
[17]
QU Y Q, WANG Q, YU Y H, et al. Comparative analysis of the complete chloroplast genome between tetraploidy and diploidy of Cyclocarya paliurus (Batal.) Iljinskaja[J]. Mitochondrial DNA B Resour, 2021, 6(9):2669-2671.DOI: 10.1080/23802359.2021.1964402.
本文引用 [1]
[18]
熊中人. 中国花楸属复叶组植物叶特征及其分类学意义[D]. 南京: 南京林业大学, 2019.
XIONG Z R. Leaf characters of Sorbus section Sorbus from China and their taxonomic significance[D]. Nanjing: Nanjing Forestry University, 2019.DOI: 10.27242/d.cnki.gnjlu.2019.000029.
本文引用 [1]
[19]
郭燕, 张树航, 李颖, 等. 燕山板栗种质资源叶片表型性状多样性研究[J]. 园艺学报, 2022, 49(8):1673-1688.
GUO Y, ZHANG S H, LI Y, et al. Diversity analysis of leaves phenotypic traits of Yanshan chestnut[J]. Acta Hortic Sin, 2022, 49(8):1673-1688.DOI: 10.16420/j.issn.0513-353x.2021-0760.
本文引用 [1]
[20]
曹林青, 钟秋平, 邹玉玲, 等. 油桐种质资源叶片结构变异及与环境因子的关系[J]. 南京林业大学学报(自然科学版), 2023, 47(4):95-102.
CAO L Q, ZHONG Q P, ZOU Y L, et al. Leaf structure variations and relationship with environmental fators among germplasm resources of Vernicia fordii[J]. J Nanjing For Univ (Nat Sci Ed), 2023, 47(4):95-102.DOI: 10.12302/j.issn.1000-2006.202108020.
本文引用 [1]
[21]
郭素娟, 武燕奇. 板栗叶片解剖结构特征及其与抗旱性的关系[J]. 西北农林科技大学学报(自然科学版), 2018, 46(9):51-59.
GUO S J, WU Y Q. Leaf anatomical structure characteristics and drought resistance of Chinese chestnut[J]. J Northwest A F Univ (Nat Sci Ed), 2018, 46(9):51-59.DOI: 10.13207/j.cnki.jnwafu.2018.09.007.
本文引用 [1]
[22]
孙清荣, 孙美娟, 孙洪雁, 等. 梨(Pyrus communis L.)多倍体新种质表型变异多样性研究[J]. 果树学报, 2016, 33(1):1-7.
SUN Q R, SUN M J, SUN H Y, et al. Diversity of phenotypic variation of polyploid plants new germplasm in pear(Pyrus communis L.)[J]. J Fruit Sci, 2016, 33(1):1-7.DOI: 10.13925/j.cnki.gsxb.20150042.
本文引用 [1]
[23]
陶抵辉. 植物多倍体的研究与应用[J]. 生物技术通报, 2010(7):22-27.
TAO D H. Studies and application on plant polyploids[J]. Biotechnol Bull, 2010(7):22-27.DOI: 10.13560/j.cnki.biotech.bull.1985.2010.07.015.
本文引用 [1]
[24]
唐军荣, 李斌, 朱丽娜, 等. 滇杨多倍体苗期叶片形态及光合生理比较分析[J]. 林业科学研究, 2016, 29(1):103-109.
TANG J R, LI B, ZHU L N, et al. Analysis on leaf morphology and photosynthesis physiology of polyploidized Populus yunnanensis seedlings[J]. For Res, 2016, 29(1):103-109.DOI: 10.13275/j.cnki.lykxyj.2016.01.015.
本文引用 [4]
[25]
刘金霞, 刘盼, 米娜瓦尔·亚森, 等. 南疆地区枣二倍体及其同源四倍体表型性状比较[J]. 西北农业学报, 2022, 31(5):595-602.
LIU J X, LIU P, Minavar Y S, et al. Comparison of phenotypic characters between diploid and autotetraploid of Chinese jujube in southern Xinjiang[J]. Acta Agric Boreali Occidentalis Sin, 2022, 31(5):595-602.DOI: 10.7606/j.issn.1004-1389.2022.05.008.
本文引用 [1]
[26]
温国, 孙皓浦, 党江波, 等. 多倍体与二倍体枇杷叶片特征及抗旱性初步分析[J]. 果树学报, 2019, 36(8):968-979.
WEN G, SUN H P, DANG J B, et al. A preliminary study on leaf characteristics and drought resistance of polyploid and diploid loquat[J]. J Fruit Sci, 2019, 36(8):968-979.DOI: 10.13925/j.cnki.gsxb.20190082.
本文引用 [2]
[27]
祁传磊, 靳春莲, 李开隆, 等. 不同倍性大青杨的光合特性及叶片解剖结构比较[J]. 植物生理学通讯, 2010, 46(9):917-922.
QI C L, JIN C L, LI K L, et al. Comparison of photosynthetic characteristics and leaf anatomy structure of different ploidy Populus ussuriensis Kom[J]. Plant Physiol Commun, 2010, 46(9):917-922.DOI: 10.13592/j.cnki.ppj.2010.09.022.
本文引用 [1]
[28]
张源源, 郭涵, 袁红章, 等. 橡胶树不同倍性的气孔性状差异研究[J]. 热带作物学报, 2017, 38(3):389-394.
ZHANG Y Y, GUO H, YUAN H Z, et al. Stomatal differences among diploid,triploid and tetraploid in rubber tree[J]. Chin J Trop Crops, 2017, 38(3):389-394.DOI: 10.3969/j.issn.1000-2561.2017.03.001.
本文引用 [2]
[29]
余文迪, 刘娟旭, 余义勋, 等. 桂中报春苣苔四倍体诱导及鉴定[J/OL]. 分子植物育种, 2023:1-12.(2023-02-10). https://kns.cnki.net/kcms/detail/46.1068.S.20230210.1133.004.html.
https://kns.cnki.net/kcms/detail/46.1068.S.20230210.1133.004.html
YU W D, LIU J X, YU Y X, et al. Induction and identification of tetraploid in Primulina guizhongensis[J/OL]. Mol Plant Breed, 2023:1-12.(2023-02-10). https://kns.cnki.net/kcms/detail/46.1068.S.20230210.1133.004.html.
https://kns.cnki.net/kcms/detail/46.1068.S.20230210.1133.004.html
本文引用 [1]
[30]
李娟娟, 邓伟, 许泽楠, 等. 黄连木多倍体诱导及鉴定[J]. 东北林业大学学报, 2022, 50(10):18-22.
LI J J, DENG W, XU Z N, et al. Induction and identification of polyploid from Pistacia chinensis[J]. J Northeast For Univ, 2022, 50(10):18-22.DOI: 10.13759/j.cnki.dlxb.2022.10.004.
本文引用 [1]
[31]
张石虎, 古敏, 周玮, 等. 黄梁木多倍体离体诱导及其性状变异[J]. 分子植物育种, 2022, 20(7):2372-2383.
ZHANG S H, GU M, ZHOU W, et al. In vitro induction of polyploid and its traits variation of Neolamarckia cadamba[J]. Mol Plant Breed, 2022, 20(7):2372-2383.DOI: 10.13271/j.mpb.020.002372.
本文引用 [1]
[32]
徐鹏飞, 杨艳红, 张毓婷, 等. 毛竹四倍体诱导及初步鉴定[J]. 林业科学, 2020, 56(8):55-62.
XU P F, YANG Y H, ZHANG Y T, et al. Induction and preliminary identification of tetraploid in Phyllostachys edulis[J]. Sci Silvae Sin, 2020, 56(8):55-62.DOI: 10.11707/j.1001-7488.20200807.
本文引用 [1]
[33]
郗连连, 李嘉宝, 朱凯琳, 等. 花楸属3种植物的基因组大小与叶气孔特征分析[J]. 植物科学学报, 2020, 38(1):32-38.
XI L L, LI J B, ZHU K L, et al. Variation in genome size and stomatal traits among three Sorbus species[J]. Plant Sci J, 2020, 38(1):32-38.DOI: 10.11913/PSJ.2095-0837.2020.10032.
本文引用 [1]
[34]
徐斌, 彭莉霞, 杨会肖, 等. 杜鹃红山茶叶片主要性状的遗传多样性分析[J]. 植物研究, 2015, 35(5):730-734.
XU B, PENG L X, YANG H X, et al. Genetic diversity analysis for leaf main traits of Camellia azalea[J]. Bull Bot Res, 2015, 35(5):730-734.DOI: 10.7525/j.issn.1673-5102.2015.05.015.
本文引用 [1]
[35]
林祺英, 李芳, 蔡汝鹏, 等. 海南荔枝资源叶片性状多样性分析[J]. 热带生物学报, 2023, 14(6):628-635.
LIN Q Y, LI F, CAI R P, et al. Diversity analysis of leaf traits of litchi genetic resources in Hainan[J]. J Trop Biol, 2023, 14(6):628-635.DOI: 10.15886/j.cnki.rdswxb.20220126.
本文引用 [1]

基金

国家自然科学基金项目(31971642)
国家自然科学基金项目(32271959)
江苏省重点研发计划(现代农业)重点项目(BE2019388)

编辑: 吴祝华
PDF(2639 KB)

Accesses

Citation

Detail
段落导航
相关文章

作者

读者

审稿

www.nldxb.njfu.edu.cn

通信地址：南京市龙蟠路南京林业大学《南京林业大学学报》编辑部
电子邮件：njlyzrb@vip.163.com
联系电话：025-85428247
邮政编码：210037
备案号：苏ICP备-010872

/

〈 〉