H2S供体NaHS对渗透胁迫下美国剑麻干旱适应性及抗氧化特性的影响

doi:10.12302/j.issn.1000-2006.202305030

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南京林业大学学报（自然科学版） ›› 2024, Vol. 48 ›› Issue (2): 121-128.doi: 10.12302/j.issn.1000-2006.202305030

H₂S供体NaHS对渗透胁迫下美国剑麻干旱适应性及抗氧化特性的影响

沈瑒(), 狄晶晶, 陈颖^*(), 冯凯, 陆锦玲, 胡宇辰

南京林业大学生命科学学院,南方现代林业协同创新中心,江苏南京 210037

收稿日期:2023-05-25 修回日期:2023-08-27 出版日期:2024-03-30 发布日期:2024-04-08
通讯作者: *陈颖(chynjfu@163.com),教授。
作者简介:沈瑒(2276582506@qq.com)。
基金资助:
国家重点研发计划(2017YFD0601301);江苏高校优势学科建设工程资助项目(PAPD)

Effects of H₂S donor NaHS on the adaptability and antioxidant properties of Agave americana plantlets under an in vitro culture of osmotic stress

SHEN Yang(), DI Jingjing, CHEN Ying^*(), FENG Kai, LU Jinling, HU Yuchen

Co-Innovation Center for Sustainable Forestry in Southern China, College of Life Sciences, Nanjing Forestry University, Nanjing 210037, China

Received:2023-05-25 Revised:2023-08-27 Online:2024-03-30 Published:2024-04-08

摘要/Abstract

摘要：

【目的】美国剑麻(Agave americana)是热带地区一种具有高经济价值的、重要的景天科酸代谢(CAM) 植物,研究其干旱适应性可为CAM植物的耐旱机制研究提供理论依据。【方法】通过对美国剑麻进行1.0 mmol/L NaHS (NS)、10% PEG(聚乙二醇, PEG10)、20% PEG (PEG20)、1.0 mmol/L NaHS+10%PEG (NS+PEG10)、1.0 mmol/L NaHS+20% PEG (NS+PEG20) 的处理,以不添加PEG和NaHS的处理为对照,研究美国剑麻组培苗对PEG渗透胁迫的响应,以及渗透胁迫下H₂S供体NaHS对其渗透调节和抗氧化特性的影响。【结果】美国剑麻组培苗在20% PEG高浓度处理下仍然存活,具有一定的抗旱能力。但植株出现了鲜质量下降(PEG20处理比对照降低了16.6%)、超微结构发生变化、丙二醛(MDA)和H₂O₂含量增加等受害症状和氧化胁迫反应。然而美国剑麻可以通过增加脯氨酸、可溶性糖的水平调节渗透压,降低胁迫强度。在高浓度PEG存在下,添加NaHS可降低脯氨酸和H₂O₂含量,从而减少脯氨酸和H₂O₂的过度积累;通过调节超氧化物歧化酶(SOD)等5种抗氧化酶活性,提高还原型谷胱甘肽(GSH)含量清除活性氧和活性羰基物,降低美国剑麻的脂质过氧化水平,增强其抗逆性。【结论】美国剑麻本身具有一定的耐旱性,H₂S能够通过调控美国剑麻的渗透调节和抗氧化水平,使其更加适应干旱的环境。

关键词: 美国剑麻, 渗透胁迫, 硫化氢, 抗旱性, 渗透调节, 抗氧化作用

Abstract:

【Objective】 Agave americana is an important crassulacean acid metabolism (CAM) plant with high economic value in tropical areas. Studying its drought adaptability could provide evidence of the drought tolerance mechanism, and support for resource development and engineering research regarding the utilization of CAM plants. 【Method】 In this study, A. americana plantlets were treated with 1.0 mmol/L NaHS (NS), 10% polyethylene glycol (PEG10), 20% PEG (PEG20), 1.0 mmol/L NaHS+10% PEG (NS+PEG10), and 1.0 mmol/L NaHS+20% PEG (NS+PEG20) under an in vitro culture. The responses to PEG osmotic stress were studied and the effects of H₂S donor NaHS on osmotic regulation and antioxidant properties in A. americana were investigated. 【Result】 The results showed that the A. americana plantlets could survive under the 20% PEG (high concentration) treatment and had a degree of drought resistance. However, injury symptoms and oxidative stress reactions occurred, with the fresh mass decreasing by 16.6% in PEG20. The cell ultrastructure changed, and the malondialdehyde (MDA) and H₂O₂ contents increased in PEG20. Despite this, the plantlets regulated osmotic pressure and reduced the stress intensity by increasing the levels of proline and soluble sugars. In the presence of PEG, H₂S donor NaHS could reduce the excessive accumulation of proline and H₂O₂. Furthermore, H₂S could activate superoxide dismutase (SOD) and five antioxidases, and increased the glutathione (GSH) content to clear active oxygen species (ROS) and active carbonyl compounds, subsequently enhancing the antioxidant capacity. 【Conclusion】 The A. americana plantlets had a certain degree of drought tolerance. The H₂S had an important role in osmotic regulation and regulating antioxidant levels, making the plantlets better adapted to drought conditions.

Key words: Agave americana, osmotic stress, H₂S, drought resistance, osmotic regulation, oxidation resistance

中图分类号:

S759.3

沈瑒,狄晶晶,陈颖,等. H₂S供体NaHS对渗透胁迫下美国剑麻干旱适应性及抗氧化特性的影响[J]. 南京林业大学学报（自然科学版）, 2024, 48(2): 121-128.

SHEN Yang, DI Jingjing, CHEN Ying, FENG Kai, LU Jinling, HU Yuchen. Effects of H₂S donor NaHS on the adaptability and antioxidant properties of Agave americana plantlets under an in vitro culture of osmotic stress[J].Journal of Nanjing Forestry University (Natural Science Edition), 2024, 48(2): 121-128.DOI: 10.12302/j.issn.1000-2006.202305030.

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