瓦雷兹芽孢杆菌HZM9对榉树生长及其根部土壤微生物的影响

杨浩业; 徐腾; 郁世军; 尚楚; 王克春; 樊奔

doi:10.12302/j.issn.1000-2006.202308042

杨浩业, 徐腾, 郁世军, 尚楚, 王克春, 樊奔

南京林业大学学报（自然科学版） ›› 2025, Vol. 49 ›› Issue (3) : 213-219.

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南京林业大学学报（自然科学版） ›› 2025, Vol. 49 ›› Issue (3) : 213-219. DOI: 10.12302/j.issn.1000-2006.202308042

研究论文

瓦雷兹芽孢杆菌HZM9对榉树生长及其根部土壤微生物的影响

杨浩业 ¹ ,
徐腾 ¹ ,
郁世军 ² ,
尚楚 ² ,
王克春 ² ,
樊奔 ¹^,^*

作者信息 +

Effects of Bacillus velezensis HZM9 on tree growth of Zelkova schneideriana and microbial communities in their root soil

YANG Haoye ¹ ,
XU Teng ¹ ,
YU Shijun ² ,
SHANG Chu ² ,
WANG Kechun ² ,
FAN Ben ¹^,^*

Author information +

文章历史 +

摘要

【目的】使用生防菌剂对尖孢镰刀菌(Fusarium oxysporum)造成的榉树(Zelkova schneideriana)枯萎病进行防治处理,研究其应用效果以及对榉树根部周围土壤微生物的影响。【方法】使用瓦雷兹芽孢杆菌(Bacillus velezensis)HZM9,对江苏句容市某种植园中遭受尖孢镰刀菌侵袭的榉树林进行抗病促生防治。一年后测定榉树胸径等生长指标,同时对榉树根部周围土壤采样,测定了土壤脲酶、碱性磷酸酶、过氧化氢酶、蔗糖酶等4种土壤酶活性以及土壤微生物的群落组成。【结果】相比于不加菌剂的对照,HZM9菌株处理使榉树胸径生长平均加快了10%,榉树的死亡率由8.7%降至5.3%,但对土壤酶活性的影响均不显著。此外,HZM9菌剂改变了土壤微生物的群落结构,显著提高了被孢霉等有益微生物的相对丰度。【结论】HZM9菌株对榉树生长具有明显抗病促生作用,同时能够显著影响榉树根部土壤微生物的群落结构,有望对榉树生产发挥长期的积极作用。

Abstract

【Objective】The study aimed to determine the effects of a biocontrol agent used to treat zelkova (Zelkova schneideriana) wilt disease caused by Fusarium oxysporum on the microorganisms in the rhizospheric soil of zelkova. 【Method】The disease control efficiency and growth promoting effects of Bacillus velezensis HZM9 on zelkova trees challenged by F. oxysporum in a plantation in Jurong City, Jiangsu Province, were determined in this study. The diameter at breast height (DBH) and death rate of the zelkova trees were analyzed after one year of treatment. Samples of rhizospheric soil were collected from the region around the roots of zelkova trees, following which the activities of four enzymes (soil urease, alkaline phosphatase, catalase, and sucrase) and the structure of the microbial community in the soil were analyzed. 【Result】Compared to that of the untreated control, B. velezensis HZM9 treatment increased the DBH of zelkova trees by 17%, and reduced their death rate from 8.7% to 5.3%. Treatment with B. velezensis HZM9 had little influence on the activities of soil enzymes but increased the soil microbial diversity. In particular, the relative abundance of the beneficial microbial groups, including Mortierella sp., improved significantly. 【Conclusion】The findings indicated that B. velezensis HZM9 can significantly promote the growth of zelkova trees and alter the structure of the soil microbial community, which may benefit the long-term production of zelkova trees.

导出引用

杨浩业, 徐腾, 郁世军, 等. 瓦雷兹芽孢杆菌HZM9对榉树生长及其根部土壤微生物的影响[J]. 南京林业大学学报（自然科学版）. 2025, 49(3): 213-219 https://doi.org/10.12302/j.issn.1000-2006.202308042

YANG Haoye, XU Teng, YU Shijun, et al. Effects of Bacillus velezensis HZM9 on tree growth of Zelkova schneideriana and microbial communities in their root soil[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2025, 49(3): 213-219 https://doi.org/10.12302/j.issn.1000-2006.202308042

中图分类号： S476.9

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