文冠果BZR1基因家族鉴定及功能分析

许慧慧; 班卓; 王晨雪; 毕泉鑫; 刘肖娟; 王利兵

doi:10.12302/j.issn.1000-2006.202403036

文冠果BZR1基因家族鉴定及功能分析

许慧慧, 班卓, 王晨雪, 毕泉鑫, 刘肖娟, 王利兵

南京林业大学学报（自然科学版） ›› 2025, Vol. 49 ›› Issue (2) : 12-22.

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南京林业大学学报（自然科学版） ›› 2025, Vol. 49 ›› Issue (2) : 12-22. DOI: 10.12302/j.issn.1000-2006.202403036

专题报道：推进乡村全面振兴视域下的多功能油用树种文冠果研究（执行主编尹佟明李维林）

文冠果BZR1基因家族鉴定及功能分析

作者信息 +

The identification and functional analysis of BZR1 genes in yellowhorn

Author information +

文章历史 +

摘要

【目的】探究文冠果(Xanthoceras sorbifolium)Brassinazole Resistance 1(BZR1)基因家族成员的特征及其在非生物胁迫响应中的作用,为文冠果XsBZR1基因功能的研究和抗逆新品种的选育提供理论参考。【方法】利用生物信息学方法鉴定并系统分析文冠果XsBZR1基因家族;构建35S::XsBZR1-eYFP融合蛋白,对XsBZR1进行亚细胞定位分析;通过实时荧光定量PCR技术分析XsBZR1基因在非生物胁迫下的表达模式;构建XsBZR1- 9基因的过表达载体并转化到拟南芥(Arabidopsis thaliana)中,观察转基因株系与野生型植株在盐胁迫处理下的生长情况。【结果】①在文冠果基因组中共鉴定出9个BZR1基因,分别命名为XsBZR1-1—XsBZR1-9,这些基因不均匀地分布在6条染色体上。②系统进化和共线性分析表明,XsBZR1蛋白与双子叶植物的BZR 1转录因子亲缘关系更为密切。③XsBZR1基因的启动子区域具有大量的光响应、激素响应元件以及胁迫应答响应元件。④亚细胞定位结果和预测结果一致,9个XsBZR1均定位于细胞核。⑤qRT-PCR分析表明,9个XsBZR1基因在不同的非生物胁迫下表现出不同的表达模式。除XsBZR1-7外,其余XsBZR1基因在低温胁迫下3 h时迅速上调表达;盐胁迫下XsBZR1的表达量呈现较大的差异性,XsBZR1-3/4/5/7的表达受到盐胁迫的显著抑制,而XsBZR1-1和XsBZR1-9在盐胁迫处理9 h时表达量提高到约20倍;XsBZR1-3/7/8/9在干旱处理9 h时表达量提高到2倍以上,而其余XsBZR1在干旱处理下的表达水平变化不大;9个XsBZR1均受到ABA的诱导表达,其中XsBZR1-8在ABA处理9 h时表达量提高到35倍。⑥在拟南芥中过表达XsBZR1-9发现,转基因植株在盐胁迫处理后的主根长度显著高于野生型植株。【结论】XsBZR1家族成员参与了文冠果非生物胁迫响应,过表达XsBZR1-9显著提高植物对盐胁迫的耐受性,这为进一步分析XsBZR1基因在文冠果抗逆机制中的作用奠定了基础。

Abstract

【Objective】This study aimed to systematically characterize the BZR1 transcription factor family in yellowhorn (Xanthoceras sorbifolium) and elucidate its functional roles in abiotic stress response, thereby providing insights for breeding stress-resistant cultivars.【Method】Genome-wide identification of XsBZR1 genes was performed using bioinformatics tools. Subcellular localization of XsBZR1 proteins was validated via 35S::XsBZR1-eYFP fusion constructs. Expression profiles under abiotic stresses (low temperature, salt, drought) and abscisic acid (ABA) treatment were analyzed by qRT-PCR. Functional validation was conducted by overexpressing XsBZR1- 9 in Arabidopsis thaliana and assessing salt tolerance phenotypes.【Result】Nine XsBZR1 genes (XsBZR1-1 to XsBZR1-9) were identified and distributed unevenly across six chromosomes. Phylogenetic and synteny analyses revealed close evolutionary relationships with dicotyledonous BZR1 orthologs. Promoter regions harbored abundant cis-regulatory elements associated with light responsiveness (48.9%), hormone signaling (35.0%) and stress adaptation (24.6%). The nuclear localization of all XsBZR1 proteins was experimentally confirmed. Differential expression patterns were observed under stress conditions: low temperature (4 ℃) rapidly induced eight XsBZR1 genes (1.3- to 6.0-fold upregulation at 3 h; P<0.05), except XsBZR1-7. Salt stress (150 mmol/L NaCl) suppressed XsBZR1-3/4/5/7 but strongly upregulated XsBZR1-1 (26.1-fold) and XsBZR1-9 (19.6-fold) at 9 h (P<0.001). Drought stress (mass fraction 25% PEG6000) elevated XsBZR1- 3/7/8/9 expression (>1.9-fold at 9 h), while others remained stable. ABA treatment (100 μmol/L) universally induced XsBZR1 genes, with XsBZR1-8 showing a 35.4-fold increase (P<0.001).Transgenic Arabidopsis overexpressing XsBZR1-9 exhibited enhanced salt tolerance, with taproot lengths twice that of the wild-type under 100 mmol/L NaCl (P<0.01).【Conclusion】The XsBZR1 gene family plays pivotal roles in yellowhorn’s abiotic stress response, with XsBZR1-9 demonstrating significant potential for improving salt tolerance. These findings advance the molecular understanding of stress adaptation mechanisms in woody plants and provide targets for precision breeding.

导出引用

许慧慧, 班卓, 王晨雪, 等. 文冠果BZR1基因家族鉴定及功能分析[J]. 南京林业大学学报（自然科学版）. 2025, 49(2): 12-22 https://doi.org/10.12302/j.issn.1000-2006.202403036

XU Huihui, BAN Zhuo, WANG Chenxue, et al. The identification and functional analysis of BZR1 genes in yellowhorn[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2025, 49(2): 12-22 https://doi.org/10.12302/j.issn.1000-2006.202403036

中图分类号： S722

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