鹅掌楸LcPIN1a基因的克隆及其对植株生长发育的影响

doi:10.12302/j.issn.1000-2006.202404005

国家林草科技领军期刊
中国精品科技期刊
中国高校百佳科技期刊
江苏省新闻出版政府奖期刊奖
RCCSE林学权威期刊（A+）
CSCD核心期刊
Scopus数据库收录期刊
中文核心期刊
SCD核心期刊

作者加群：102861116

微信公众号：南京林业大学学报

高级检索

南京林业大学学报（自然科学版） ›› 2024, Vol. 48 ›› Issue (6): 51-61.doi: 10.12302/j.issn.1000-2006.202404005

鹅掌楸LcPIN1a基因的克隆及其对植株生长发育的影响

郝兆东(), 马筱筱, 王丹丹, 陆叶, 施季森, 陈金慧^*()

林木遗传育种全国重点实验室,南方现代林业协同创新中心,南京林业大学林草学院,江苏南京 210037

收稿日期:2024-04-02 修回日期:2024-05-21 出版日期:2024-11-30 发布日期:2024-12-10
通讯作者: *陈金慧(chenjh@njfu.edu.cn),教授。
作者简介:
郝兆东(haozd@njfu.edu.cn),博士,讲师。
基金资助:
国家自然科学基金面上项目(32071784)

Cloning of the Liriodendron chinense LcPIN1a genes and its effect on plant growth and development

HAO Zhaodong(), MA Xiaoxiao, WANG Dandan, LU Ye, SHI Jisen, CHEN Jinhui^*()

State Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry and Grassland, Nanjing Forestry University, Nanjing 210037, China

Received:2024-04-02 Revised:2024-05-21 Online:2024-11-30 Published:2024-12-10

摘要/Abstract

摘要：

【目的】PIN-FORMED(PIN)属于生长素转运蛋白,能够介导生长素的极性运输,在植物的生长和发育过程中扮演着关键角色。本研究旨在解析鹅掌楸PIN1基因对植物生长和发育的影响。【方法】通过蛋白序列同源比对、系统发育树构建以及蛋白结构域预测的方法,鉴定鹅掌楸中PIN1的同源蛋白LcPIN1s。然后,通过转录组数据解析LcPIN1s基因的组织特异性表达情况,并通过实时荧光定量逆转录PCR(RT-qPCR)方法研究LcPIN1s在不同苗龄再生植株的根、茎和叶组织中的表达动态。此外,通过转录组数据解析LcPIN1s基因在低温、高温以及干旱胁迫条件下的时序动态表达,并进一步利用RT-qPCR方法研究LcPIN1s响应干旱胁迫与内源ABA合成之间的关联。最后,通过克隆鹅掌楸叶片中高表达的PIN1同源基因LcPIN1a,构建由CaMV35S启动子驱动的过表达载体(35S:LcPIN1a),通过异源转化拟南芥(Arabidopsis thaliana)和同源转化杂种鹅掌楸(Liriodendron × sinoamericanum),筛选并获得的异源过表达(LcPIN1a-HO)和同源过表达(LcPIN1a-OE)阳性再生植株,分别进行生长和发育性状的测定和分析。【结果】通过生物信息学方法在鹅掌楸基因组中成功鉴定到了3个PIN1同源蛋白,分别命名为LcPIN1a、LcPIN1b和LcPIN1c。组织特异性表达分析显示,LcPIN1a主要在叶片中高表达,而LcPIN1b和LcPIN1c主要在茎和根以及成年后的雌蕊中高表达。另外,鹅掌楸3个PIN1同源基因均受到低温(4 ℃)和干旱(质量分数15% PEG6000)处理的诱导而表现出先上调后下调的表达模式,但在高温(40 ℃)胁迫下则会急剧下调表达。利用聚乙二醇(PEG)、脱落酸(ABA)以及ABA合成抑制剂氟啶酮(Flu)处理,发现LcPIN1s在响应干旱诱导时表现出不同的模式,即LcPIN1a不依赖于内源ABA的合成,而LcPIN1b和LcPIN1c则依赖于内源ABA的合成。最后,通过对拟南芥异源过表达株系(LcPIN1a-HO)再生植株的生长性状统计分析发现,其在根长和株高方面均显著低于野生型,同时雄蕊数发生显著变异,从野生型的6枚雄蕊变为以5枚为主。在杂交鹅掌楸过表达株系(LcPIN1a-OE)中,其体胚成苗率显著降低,并且成苗后的再生植株在根长和株高方面均显著低于野生型,同时根系结构发生明显变化,主根不明显。【结论】鹅掌楸PIN1蛋白在植株的营养和生殖生长方面发挥重要作用,过量表达不利于植株的正常生长和发育。

关键词: 鹅掌楸, 生长素转运蛋白, PIN1基因, 生长发育

Abstract:

【Objective】This study aimed to explore the role of the auxin transporter PIN1 in plant growth and development in Liriodendron chinense.【Method】Three PIN1 homologous proteins were identified in the Liriodendron genome using bioinformatic methods, and expression pattern analyses of the three LcPIN1s genes were performed in different tissues and response to various abiotic stresses. An overexpression vector driven by the CaMV35S promoter was then constructed and transformed into Arabidopsis and Liriodendron×sinoamericanum, followed by phenotypic determination of growth and developmental traits in the transgenic positive lines.【Result】Three PIN1 homologous proteins were identified in the Liriodendron genome, named LcPIN1a, LcPIN1b and LcPIN1c. Expression pattern analyses showed that LcPIN1a was mainly expressed in leaves, while LcPIN1b and LcPIN1c were primarily expressed in roots and stems and stigmas when the plantlets transitioned into reproductive growth. In addition, all three LcPIN1 genes transcriptionally responded to drought stress, with LcPIN1b and LcPIN1c showing dependence on the biosynthesis of endogenous ABA, while LcPIN1a does not. Root length and plant height were significantly reduced in LcPIN1a-heterologous overexpression (LcPIN1a-HO) lines compared to wild-type Arabidopsis. The number of stamens was predominantly five in LcPIN1a-HO lines, whereas wild-type Arabidopsis typically contained six stamens. The regeneration of plantlets in LcPIN1a-overexpressing (LcPIN1a-OE) Liriodendron×sinoamericanum was significantly reduced compared to wild-type plants. In addition, the root length and plant height of LcPIN1a-OE regenerated seedlings were significantly lower than those of the wild type. The root structure of LcPIN1a-OE plants was significantly changed, with the taproot being less distinct.【Conclusion】The PIN1 proteins of L. chinense play a crucial role in vegetative and reproductive growth. Overexpression of LcPIN1 genes can be detrimental to normal plant growth and development.

Key words: Liriodendron chinense, auxin transporter, PIN1 gene, growth and development

中图分类号:

S718
S68

郝兆东,马筱筱,王丹丹,等. 鹅掌楸LcPIN1a基因的克隆及其对植株生长发育的影响[J]. 南京林业大学学报（自然科学版）, 2024, 48(6): 51-61.

HAO Zhaodong, MA Xiaoxiao, WANG Dandan, LU Ye, SHI Jisen, CHEN Jinhui. Cloning of the Liriodendron chinense LcPIN1a genes and its effect on plant growth and development[J].Journal of Nanjing Forestry University (Natural Science Edition), 2024, 48(6): 51-61.DOI: 10.12302/j.issn.1000-2006.202404005.

图/表 7

表1

图1

图2

图3

图4

图5

图6

参考文献 56

[1]	ZHAO Y D. Auxin biosynthesis and its role in plant development[J]. Annu Rev Plant Biol, 2010,61:49-64.DOI: 10.1146/annurev-arplant-042809-112308.
[2]	FUKUI K, HAYASHI K I. Manipulation and sensing of auxin metabolism,transport and signaling[J]. Plant Cell Physiol, 2018, 59(8):1500-1510.DOI: 10.1093/pcp/pcy076.
[3]	NARAMOTO S. Polar transport in plants mediated by membrane transporters:focus on mechanisms of polar auxin transport[J]. Curr Opin Plant Biol, 2017, 40:8-14.DOI: 10.1016/j.pbi.2017.06.012.
[4]	TAN S T, LUSCHNIG C, FRIML J. Pho-view of auxin:reversible protein phosphorylation in auxin biosynthesis,transport and signaling[J]. Mol Plant, 2021, 14(1):151-165.DOI: 10.1016/j.molp.2020.11.004.
[5]	ADAMOWSKI M, FRIML J. PIN-dependent auxin transport:action,regulation,and evolution[J]. Plant Cell, 2015, 27(1):20-32.DOI: 10.1105/tpc.114.134874.
[6]	BLILOU I, XU J, WILDWATER M, et al. The PIN auxin efflux facilitator network controls growth and patterning in Arabidopsis roots[J]. Nature, 2005, 433(7021):39-44.DOI: 10.1038/nature03184.
[7]	FRIML J, BENKOVÁ E, BLILOU I, et al. AtPIN4 mediates sink-driven auxin gradients and root patterning in Arabidopsis[J]. Cell, 2002, 108(5):661-673.DOI: 10.1016/s0092-8674(02)00656-6.
[8]	GÄLWEILER L, GUAN C, MÜLLER A, et al. Regulation of polar auxin transport by AtPIN1 in Arabidopsis vascular tissue[J]. Science, 1998, 282(5397):2226-2230.DOI: 10.1126/science.282.5397.2226.
[9]	FRIML J, VIETEN A, SAUER M, et al. Efflux-dependent auxin gradients establish the apical-basal axis of Arabidopsis[J]. Nature, 2003, 426(6963):147-153.DOI: 10.1038/nature02085.
[10]	MÜLLER A, GUAN C, GÄLWEILER L, et al. AtPIN2 defines a locus of Arabidopsis for root gravitropism control[J]. EMBO J, 1998, 17(23):6903-6911.DOI: 10.1093/emboj/17.23.6903.
[11]	FRIML J, WISNIEWSKA J, BENKOVÁ E, et al. Lateral relocation of auxin efflux regulator PIN3 mediates tropism in Arabidopsis[J]. Nature, 2002, 415(6873):806-809.DOI: 10.1038/415806a.
[12]	DING Z J, WANG B J, MORENO I, et al. ER-localized auxin transporter PIN8 regulates auxin homeostasis and male gametophyte development in Arabidopsis[J]. Nat Commun, 2012,3:941.DOI: 10.1038/ncomms1941.
[13]	SIMON S, SKŮPA P, VIAENE T, et al. PIN6 auxin transporter at endoplasmic reticulum and plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsis[J]. New Phytol, 2016, 211(1):65-74.DOI: 10.1111/nph.14019.
[14]	OKADA K, UEDA J, KOMAKI M K, et al. Requirement of the auxin polar transport system in early stages of Arabidopsis floral bud formation[J]. Plant Cell, 1991, 3(7):677-684.DOI: 10.1105/tpc.3.7.677.
[15]	GOVINDARAJU P, VERNA C, ZHU T B, et al. Vein patterning by tissue-specific auxin transport[J]. Development, 2020, 147(13):dev187666.DOI: 10.1242/dev.187666.
[16]	NOH B, BANDYOPADHYAY A, PEER W A, et al. Enhanced gravi-and phototropism in plant mdr mutants mislocalizing the auxin efflux protein PIN1[J]. Nature, 2003, 423(6943):999-1002.DOI: 10.1038/nature01716.
[17]	LI Y, ZHU J S, WU L L, et al. Functional divergence of PIN1 paralogous genes in rice[J]. Plant Cell Physiol, 2019, 60(12):2720-2732.DOI: 10.1093/pcp/pcz159.
[18]	郝日明, 贺善安, 汤诗杰, 等. 鹅掌楸在中国的自然分布及其特点[J]. 植物资源与环境, 1995, 4(1):1-6.
	HAO R M, HE S A, TANG S J, et al. Geographical distribution of Liridendron chinense in China and its significance[J]. J Plant Resour Environ, 1995, 4(1):1-6.
[19]	PARKS C R, MILLER N G, WENDEL J F, et al. Genetic divergence within the genus Liriodendron (Magnoliaceae)[J]. Ann Mo Bot Gard, 1983, 70(4):658.DOI: 10.2307/2398983.
[20]	PARKS C R, WENDEL J F. Molecular divergence between Asian and north American species of Liriodendron (Magnoliaceae) with implications for interpretation of fossil floras[J]. Am J Bot, 1990, 77(10):1243.DOI: 10.2307/2444585.
[21]	李火根, 施季森. 杂交鹅掌楸良种选育与种苗繁育[J]. 林业科技开发, 2009, 23(3):1-5.
	LI H G, SHI J S. Breeding and propagation of Liriodendron hybrids[J]. J For Eng, 2009, 23(3):1-5.DOI: 10.3969/j.issn.1000-8101.2009.03.001.
[22]	向其柏, 王章荣. 杂交马褂木的新名称:亚美马褂木[J]. 南京林业大学学报(自然科学版), 2012, 36(2):1-2.
	SHANG C B, WANG Z R. A new scientific name of hybrid Liriodendron: L. sino americanum[J]. J Nanjing For Univ (Nat Sci Ed), 2012, 36(2):1-2.DOI: 10.3969/j.issn.1000-2006.2012.02.001.
[23]	CHEN J H, HAO Z D, GUANG X M, et al. Liriodendron genome sheds light on angiosperm phylogeny and species-pair differentiation[J]. Nat Plants, 2019, 5(1):18-25.DOI: 10.1038/s41477-018-0323-6.
[24]	陈金慧, 施季森, 诸葛强, 等. 杂交鹅掌楸体细胞胚胎发生研究[J]. 林业科学, 2003, 39(4):49-53,177.
	CHEN J H, SHI J S, ZHUGE Q, et al. Studies on the somatic embryogenesis of Liriodendron hybrids (L. chinense × L. tulipifera)[J]. Sci Silvae Sin, 2003, 39(4):49-53,177.DOI: 10.3321/j.issn:1001-7488.2003.04.008.
[25]	LI M P, WANG D, LONG X F, et al. Agrobacterium-mediated genetic transformation of embryogenic callus in a Liriodendron hybrid (L.chinense × L.tulipifera)[J]. Front Plant Sci, 2022,13:802128.DOI: 10.3389/fpls.2022.802128.
[26]	HU L F, WANG P K, LONG X F, et al. The PIN gene family in relic plant L.chinense:genome-wide identification and gene expression profiling in different organizations and abiotic stress responses[J]. Plant Physiol Biochem, 2021, 162:634-646.DOI: 10.1016/j.plaphy.2021.03.030.
[27]	LI R, PAN Y, HU L F, et al. PIN3 from Liriodendron may function in inflorescence development and root elongation[J]. Forests, 2022, 13(4):568.DOI: 10.3390/f13040568.
[28]	BERARDINI T Z, REISER L, LI D H, et al. The Arabidopsis information resource:making and mining the “gold standard” annotated reference plant genome[J]. Genesis, 2015, 53(8):474-485.DOI: 10.1002/dvg.22877.
[29]	JOHNSON M, ZARETSKAYA I, RAYTSELIS Y, et al. NCBI BLAST:a better web interface[J]. Nucleic Acids Res, 2008, 36(Web Server issue):5-9.DOI: 10.1093/nar/gkn201.
[30]	GOODSTEIN D M, SHU S Q, HOWSON R, et al. Phytozome:a comparative platform for green plant genomics[J]. Nucleic Acids Res, 2012, 40(Database issue):D1178-D1186.DOI: 10.1093/nar/gkr944.
[31]	SIEVERS F, HIGGINS D G. Clustal Omega for making accurate alignments of many protein sequences[J]. Protein Sci, 2018, 27(1):135-145.DOI: 10.1002/pro.3290.
[32]	TOGKOUSIDIS A, KOZLOV O M, HAAG J, et al. Adaptive RAxML-NG:accelerating phylogenetic inference under maximum likelihood using dataset difficulty[J]. Mol Biol Evol, 2023, 40(10):msad227.DOI: 10.1093/molbev/msad227.
[33]	CHEN C J, WU Y, LI J W, et al. TBtools-Ⅱ:a“one for all,all for one” bioinformatics platform for biological big-data mining[J]. Mol Plant, 2023, 16(11):1733-1742.DOI: 10.1016/j.molp.2023.09.010.
[34]	LETUNIC I, BORK P. Interactive tree of life (iTOL) v5:an online tool for phylogenetic tree display and annotation[J]. Nucleic Acids Res, 2021, 49(W1):293-296.DOI: 10.1093/nar/gkab301.
[35]	OMASITS U, AHRENS C H, MÜLLER S, et al. Protter:interactive protein feature visualization and integration with experimental proteomic data[J]. Bioinformatics, 2014, 30(6):884-886.DOI: 10.1093/bioinformatics/btt607.
[36]	LI T T, YUAN W G, QIU S, et al. Selection of reference genes for gene expression analysis in Liriodendron hybrids’ somatic embryogenesis and germinative tissues[J]. Sci Rep, 2021, 11(1):4957.DOI: 10.1038/s41598-021-84518-w.
[37]	LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2 (-Delta Delta C(T)) Method[J]. Methods, 2001, 25(4):402-408.DOI: 10.1006/meth.2001.1262.
[38]	MAVRODIEV E V, DERVINIS C, WHITTEN W M, et al. A new,simple,highly scalable,and efficient protocol for genomic DNA extraction from diverse plant taxa[J]. Appl Plant Sci, 2021, 9(3):e11413.DOI: 10.1002/aps3.11413.
[39]	CLOUGH S J, BENT A F. Floral dip:a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana[J]. Plant J, 1998, 16(6):735-743.DOI: 10.1046/j.1365-313x.1998.00343.x.
[40]	KRECEK P, SKUPA P, LIBUS J, et al. The PIN-FORMED (PIN) protein family of auxin transporters[J]. Genome Biol, 2009, 10(12):249.DOI: 10.1186/gb-2009-10-12-249.
[41]	SANCHO-ANDRÉS G, SORIANO-ORTEGA E, GAO C J, et al. Sorting motifs involved in the trafficking and localization of the PIN1 auxin efflux carrier[J]. Plant Physiol, 2016, 171(3):1965-1982.DOI: 10.1104/pp.16.00373.
[42]	MARCOTE M J, SANCHO-ANDRÉS G, SORIANO-ORTEGA E, et al. Sorting signals for PIN1 trafficking and localization[J]. Plant Signal Behav, 2016, 11(8):e1212801.DOI: 10.1080/15592324.2016.1212801.
[43]	HUANG F, ZAGO M K, ABAS L, et al. Phosphorylation of conserved PIN motifs directs Arabidopsis PIN1 polarity and auxin transport[J]. Plant Cell, 2010, 22(4):1129-1142.DOI: 10.1105/tpc.109.072678.
[44]	WU W H, ZHU S, XU L, et al. Genome-wide identification of the Liriodendron chinense WRKY gene family and its diverse roles in response to multiple abiotic stress[J]. BMC Plant Biol, 2022, 22(1):25.DOI: 10.1186/s12870-021-03371-1.
[45]	WU W H, ZHU S, ZHU L M, et al. Characterization of the Liriodendron chinense MYB gene family and its role in abiotic stress response[J]. Front Plant Sci, 2021,12:641280.DOI: 10.3389/fpls.2021.641280.
[46]	MROUE S, SIMEUNOVIC A, ROBERT H S. Auxin production as an integrator of environmental cues for developmental growth regulation[J]. J Exp Bot, 2018, 69(2):201-212.DOI: 10.1093/jxb/erx259.
[47]	GU Z L, STEINMETZ L M, GU X, et al. Role of duplicate genes in genetic robustness against null mutations[J]. Nature, 2003, 421(6918):63-66.DOI: 10.1038/nature01198.
[48]	TESSI T M, MAURINO V G, SHAHRIARI M, et al. AZG1 is a cytokinin transporter that interacts with auxin transporter PIN1 and regulates the root stress response[J]. New Phytol, 2023, 238(5):1924-1941.DOI: 10.1111/nph.18879.
[49]	BAWA G, LIU Z X, WU R, et al. PIN1 regulates epidermal cells development under drought and salt stress using single-cell analysis[J]. Front Plant Sci, 2022,13:1043204.DOI: 10.3389/fpls.2022.1043204.
[50]	ZHANG J, VANNESTE S, BREWER P B, et al. Inositol trisphosphate-induced Ca²⁺ signaling modulates auxin transport and PIN polarity[J]. Dev Cell, 2011, 20(6):855-866.DOI: 10.1016/j.devcel.2011.05.013.
[51]	XU M, ZHU L, SHOU H X, et al. A PIN1 family gene,OsPIN1,involved in auxin-dependent adventitious root emergence and tillering in rice[J]. Plant Cell Physiol, 2005, 46(10):1674-1681.DOI: 10.1093/pcp/pci183.
[52]	MRAVEC J, KUBES M, BIELACH A, et al. Interaction of PIN and PGP transport mechanisms in auxin distribution-dependent development[J]. Development, 2008, 135(20):3345-3354.DOI: 10.1242/dev.021071.
[53]	LI K, KAMIYA T, FUJIWARA T. Differential roles of PIN1 and PIN2 in root meristem maintenance under low-B conditions in Arabidopsis thaliana[J]. Plant Cell Physiol, 2015, 56(6):1205-1214.DOI: 10.1093/pcp/pcv047.
[54]	XU K J, SUN F L, WANG Y F, et al. The PIN1 family gene PvPIN1 is involved in auxin-dependent root emergence and tillering in switchgrass[J]. Genet Mol Biol, 2016, 39(1):62-72.DOI: 10.1590/1678-4685-GMB-2014-0300.
[55]	GUAN L, LI Y J, HUANG K H, et al. Auxin regulation and MdPIN expression during adventitious root initiation in apple cuttings[J]. Hortic Res, 2020, 7(1):143.DOI: 10.1038/s41438-020-00364-3.
[56]	ROTH O, YECHEZKEL S, SERERO O, et al. Slow release of a synthetic auxin induces formation of adventitious roots in recalcitrant woody plants[J]. Nat Biotechnol, 2024.DOI: 10.1038/s41587-023-02065-3.

引物名称 primer name	引物序列 primer sequence	用途 purpose
LcPIN1a-qF	AAGAGCCGCAAGAAGAGT	实时荧光定量
LcPIN1a-qR	TGTTTGGATTACGGATTAGC
LcPIN1b-qF	TACCCGGCACCAAATCCAG
LcPIN1b-qR	CGCCTATGTATTCTTGATTCCC
LcPIN1c-qF	GGGAGGAACACGAGCAAT
LcPIN1c-qR	CTTCTTCTTCGCCACAGC
LcPIN1a-pF	ATGATCACTCTCTCCGACTTC	基因克隆
LcPIN1a-pR	CAGCCCAAGGAAAATGTAGTAAAC
LcPIN1a-vF	gagagaacacgggggactctaga ATGATCACTCTCTCCGACTTC	过表达载体构建
LcPIN1a-vR	gatcggggaaattcgagctc CAGCCCAAGGAAAATGTAGTAAAC
LcPIN1a-sF	CAGGTCCCCAGATTAGCCTT	载体验证
LcPIN1a-sR	ACATGAAGCTTACCGTCCTCC

鹅掌楸LcPIN1a基因的克隆及其对植株生长发育的影响

Cloning of the Liriodendron chinense LcPIN1a genes and its effect on plant growth and development

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 7

参考文献 56

相关文章 15

编辑推荐

Metrics

本文评价

作者

读者

审稿

[1]	尹增芳, 欧香, 陈瑶, 杨爱香, 孙李勇. 望春玉兰生物学基础研究进展与展望[J]. 南京林业大学学报（自然科学版）, 2024, 48(2): 256-262.
[2]	魏绪英, 张瑶, 马美霞, 姜雪茹, 陈慧婷, 吴靖, 杨玉, 蔡军火. 石蒜年生长周期内NSC及其代谢酶活性变化[J]. 南京林业大学学报（自然科学版）, 2024, 48(1): 106-114.
[3]	张恒, 陈锐帆, 林嘉蓓, 邓小梅, 奚如春. 微量元素在林木中的应用研究进展[J]. 南京林业大学学报（自然科学版）, 2022, 46(5): 229-239.
[4]	陈菊艳, 邓伦秀, 李鹤, 龙海燕, 徐超然. 遮光对贵州原产两种金花茶生长发育和生理特性的影响[J]. 南京林业大学学报（自然科学版）, 2022, 46(3): 83-90.
[5]	万雅雯, 傅华君, 时培建, 林树燕. 变温对毛竹种子萌发及幼苗生长的影响[J]. 南京林业大学学报（自然科学版）, 2021, 45(4): 97-106.
[6]	姚文静, 王茹, 林树燕, 王星, 杨蒙, 郑毅, 丁雨龙. 翠竹实生苗生长发育规律及构件生物量模型拟合研究[J]. 南京林业大学学报（自然科学版）, 2020, 44(6): 103-110.
[7]	贾志怡, 陈聪, 马宇萱, 李寿银, 樊斌琦, 王焱, 郝德君. 温度对香樟齿喙象生长发育的影响[J]. 南京林业大学学报（自然科学版）, 2020, 44(4): 131-136.
[8]	陆叶,龙晓飞,王鹏凯,陈金慧,施季森. 基于RAD-seq技术的鹅掌楸基因组SNP标记开发[J]. 南京林业大学学报（自然科学版）, 2019, 43(04): 1-7.
[9]	杨海燕,董珊珊,闾连飞,黄正金,吴文龙,李维林. 三个高丛蓝莓品种在南京地区的田间生长表现[J]. 南京林业大学学报（自然科学版）, 2019, 43(03): 157-162.
[10]	时培建,刘梦菂. 鹅掌楸属植物叶片两侧对称性测量的泰勒幂法则[J]. 南京林业大学学报（自然科学版）, 2019, 43(03): 145-151.
[11]	潘文婷,夏莘,夏良放,孙建军,武晓玉,余良富,厉月桥. 造林密度对近熟期鹅掌楸生长和材质的影响[J]. 南京林业大学学报（自然科学版）, 2018, 42(05): 46-52.
[12]	成铁龙,孟岩1,3,陈金慧1,3,施季森. 茉莉酸甲酯对杂交鹅掌楸体胚发育的影响[J]. 南京林业大学学报（自然科学版）, 2017, 41(06): 41-46.
[13]	鲁路,陆叶,盛宇,郑晨,施季森,陈金慧. 不同活性炭对杂交鹅掌楸体胚发生的影响[J]. 南京林业大学学报（自然科学版）, 2016, 40(02): 59-64.
[14]	赵亚琦,吕言,张文军,魏继福,陈金慧,施季森,成铁龙. 鹅掌楸树叶和树皮提取物的抑菌活性研究[J]. 南京林业大学学报（自然科学版）, 2016, 40(02): 76-80.
[15]	石江涛,王丰,骆嘉言. 杂交鹅掌楸应力木解剖特征及光谱分析[J]. 南京林业大学学报（自然科学版）, 2015, 39(03): 125-129.