资源植物黑老虎的比较叶绿体基因组学研究

doi:10.12302/j.issn.1000-2006.202404014

国家林草科技领军期刊
中国精品科技期刊
中国高校百佳科技期刊
江苏省新闻出版政府奖期刊奖
RCCSE林学权威期刊（A+）
CSCD核心期刊
Scopus数据库收录期刊
中文核心期刊
SCD核心期刊

作者加群：102861116

微信公众号：南京林业大学学报

高级检索

南京林业大学学报（自然科学版） ›› 2024, Vol. 48 ›› Issue (6): 71-78.doi: 10.12302/j.issn.1000-2006.202404014

资源植物黑老虎的比较叶绿体基因组学研究

翟学昌¹(), 彭丽¹, 颜海飞²^,^*(), 朱柯帆³, 张淑燕²^,⁴, 张彩云⁵, 鲁显楷²

1.江西环境工程职业学院,江西赣州 341000
2.中国科学院华南植物园,广东省应用植物学重点实验室,广东广州 510650
3.信丰县林木良种场,江西赣州 341624
4.华南农业大学生命科学学院,广东广州 510642
5.广东食品药品职业学院,广东广州 510520

收稿日期:2024-04-10 修回日期:2024-09-05 出版日期:2024-11-30 发布日期:2024-12-10
通讯作者: *颜海飞(yanhaifei@scbg.ac.cn),副研究员。
作者简介:
翟学昌(xuechangzhai@163.com),副教授。
基金资助:
江西省教育厅科学技术研究项目(171271)

Comparative chloroplast genomics of the important resource plant Kadsura coccinea

ZHAI Xuechang¹(), PENG Li¹, YAN Haifei²^,^*(), ZHU Kefan³, ZHANG Shuyan²^,⁴, ZHANG Caiyun⁵, LU Xiankai²

1. Jiangxi Environmental Engineering Vocational College, Ganzhou 341000, China
2. Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China
3. Xinfeng County Seed Farm of Jiangxi Province, Ganzhou 341624, China
4. College of Life Sciences, South China Agricultural University, Guangzhou 510642, China
5. Guangdong Food and Drug Vocational College, Guangzhou 510520, China

Received:2024-04-10 Revised:2024-09-05 Online:2024-11-30 Published:2024-12-10

摘要/Abstract

摘要：

【目的】发掘我国重要资源植物黑老虎(Kadsura coccinea)的叶绿体基因组高变异区域及SSR变异位点,为其遗传多样性及种质资源评估奠定基础。【方法】获取5个黑老虎个体的叶绿体基因组,通过基因注释,采用生物信息学方法开展核苷酸多态性、简单重复序列(simple sequarce repeat,SSR)分析,并以2个五味子属植物为外类群分析南五味子属的系统发育关系。【结果】黑老虎完整叶绿体基因组为典型的四分体环状结构,长度为145 413~145 903 bp。其中,大单拷贝区的长度范围为94 457~94 757 bp,小单拷贝区的长度为18 032~18 047 bp,重复区为16 431~16 552 bp。黑老虎叶绿体基因组编码125个基因,蛋白编码基因、tRNA基因、rRNA基因分别为82、35、8个;总G(鸟嘌呤)和C(胞嘧啶)的碱基数占总碱基数的比例(GC占比)为39.7%。采用滑动窗口法分析发现黑老虎叶绿体基因组上petN-psbM和trnS-GCU-trnG-UCC具有很高的核苷酸多态性(P_i > 0.03)。该物种叶绿体基因组上共有212个SSR位点,其中24个SSR位点在5个黑老虎个体间存在多态变异,具有应用前景。系统发育分析表明,此次研究中黑老虎个体聚成一支,与同属其他物种关系较远。【结论】本研究首次系统比较了多个黑老虎个体的叶绿体基因组,发掘出基因组内的高变异区域及变异SSR位点,为其遗传多样性及种质资源评估奠定了基础。

关键词: 黑老虎, 质体基因组, 遗传多样性, SSR位点, 系统发育

Abstract:

【Objective】This study aims to investigate the chloroplast genomes and SSR loci of Kadsura coccinea, an important plant resource in China, to establish a basis for assessing its genetic diversity and germplasm resources.【Method】The study obtained and analyzed the chloroplast genomes of five individuals of K. coccinea through genetical annotation, nucleotide polymorphism analysis, and SSR analysis using bioinformatics methods. Additionally, the phylogenetic analysis of Kadsura were reconstructed using two Schisandra spp. as outgroups.【Result】The chloroplast genome of K. coccinea showed a typical quadripartite structure, with genome lengths ranging from 145 413 to 145 903 base pairs (bp). The large single-copy region (LSC) spaned from 94 457 to 94 757 bp, while the small single-copy region (SSC) encompassed 18 032 to 18 047 bp. It encodes a total of 125 genes, including 82 protein-coding genes, 35 tRNA genes, and 8 rRNA genes. The genome had a total GC content of 39.7% and demonstrated substantial nucleotide polymorphisms (P_i > 0.03) in the intergenic regions of petN-psbM and trnS-GCU-trnG-UCC. A total of 212 single sequence repeat (SSR) loci were identified across the chloroplast genomes of this species. Mononucleotide repeats were the most prevalent, followed by trinucleotide repeats, and pentanucleotide repeats were the least frequent. Among these loci, 24 polymorphic SSR loci were found among five individuals of K. coccinea, indicating their potential utility in future. Phylogenetic analysis robustly clusters K. coccinea individuals into a distinct group, revealing no close relationship with other congeneric species.【Conclusion】This study presents the first systematic comparison of chloroplast genomes among multiple K. coccinea individuals. Our findings identified highly variable regions and SSR loci that can be valuable for evaluating the genetic diversity and germplasm resource of this species.

Key words: Kadsura coccinea, plastid genome, genetic diversity, SSR loci, phylogeny

中图分类号:

S718
Q754

翟学昌,彭丽,颜海飞,等. 资源植物黑老虎的比较叶绿体基因组学研究[J]. 南京林业大学学报（自然科学版）, 2024, 48(6): 71-78.

ZHAI Xuechang, PENG Li, YAN Haifei, ZHU Kefan, ZHANG Shuyan, ZHANG Caiyun, LU Xiankai. Comparative chloroplast genomics of the important resource plant Kadsura coccinea[J].Journal of Nanjing Forestry University (Natural Science Edition), 2024, 48(6): 71-78.DOI: 10.12302/j.issn.1000-2006.202404014.

图/表 6

图1

表1

表2

图2

图3

图4

参考文献 50

[1]	XIA N H, LIU Y H, SOUNDERS R M K. Schisandraceae[C]// WU Z Y, RAVEN P H, HONG D Y. Floral of China. Beijing: Science Press, 2008: 39-47.
[2]	林祁, 段林东, 姚炳矾. 南五味子属(五味子科)三种植物之补记[J]. 植物分类学报, 2005, 43(6):567-570.
	LIN Q, DUAN L D, YAO B F. Notes on three species of the genus Kadsura Juss.(Schisandraceae)[J]. Acta Phytotaxon Sin, 2005, 43(6):567-570.
[3]	黄珊珊, 黄晓玲, 宋卉, 等. 中药黑老虎的研究进展[J]. 海峡药学, 2021, 33(11):38-40.
	HUANG S S, HUANG X L, SONG H, et al. Advances in traditional Chinese medicine Kadsura coccinea[J]. Strait Pharm J, 2021, 33(11):38-40.DOI: 10.3969/j.issn.1006-3765.2021.11.012.
[4]	苏维, 王欣悦, 付港, 等. 南五味子属植物的化学成分、药理作用及临床应用研究进展[J]. 中国中药杂志, 2024, 49(1):26-38.
	SU W, WANG X Y, FU G, et al. Research progress on chemical constituents from Kadsura genus and its pharmacological activities and clinical application[J]. China J Chin Mater Med, 2024, 49(1):26-38.DOI: 10.19540/j.cnki.cjcmm.20230718.201.
[5]	杨赛男, 戴斌, 潘清平, 等. 黑老虎植物资源利用研究进展[J]. 湖南生态科学学报, 2022, 9(3):112-120.
	YANG S N, DAI B, PAN Q P, et al. Research progress on the application value and comprehensive utilization of Kadsura coccinea resources[J]. J Hunan Ecol Sci, 2022, 9(3):112-120.DOI: 10.3969/j.issn.2095-7300.2022.03.015.
[6]	杨芝干. 地标奇果:通道“黑老虎”[J]. 生命世界, 2019(9):66-69.
	YANG Z G. Landmark fruit-tongdao “Black Tiger”[J]. Life World, 2019(9):66-69.
[7]	林旭俊, 陆文, 李善志, 等. 药用植物黑老虎的资源调查[J]. 热带林业, 2019, 47(2):34-36.
	LIN X J, LU W, LI S Z, et al. Investigation on the resources of medicinal plant Kadsura coccinea[J]. Trop For, 2019, 47(2):34-36.DOI: 10.3969/j.issn.1672-0938.2019.02.009.
[8]	韦霄, 梁惠凌, 唐辉, 等. 广西南五味子属植物的分布与利用[J]. 广西农业科学, 2006, 37(2):117-119.
	WEI X, LIANG H L, TANG H, et al. Distribution and utilization of Kadsura in Guangxi[J]. Guangxi Agric Sci, 2006, 37(2):117-119.DOI: 10.3969/j.issn.2095-1191.2006.02.006.
[9]	邹建文, 罗先权, 饶红欣, 等. 常绿木质藤本植物黑老虎基因组SSR特征分析及引物开发[J]. 中南林业科技大学学报, 2021, 41(4):130-138.
	ZOU J W, LUO X Q, RAO H X, et al. Characters of genomic SSRs and development of 28 SSR markers for Kadsura coccinea,an evergreen woody vine[J]. J Cent South Univ For Technol, 2021, 41(4):130-138.DOI: 10.14067/j.cnki.1673-923x.2021.04.015.
[10]	ZOU J W, HE R H, RAO H X, et al. Genetic diversity and population genetic structure in Kadsura coccinea (Schisandraceae),an evergreen woody vine from Hunan,China[J]. J For Res, 2023, 28(5):364-373.DOI: 10.1080/13416979.2023.2220192.
[11]	DONG Y Q, WEI X P, QIANG T Y, et al. RAD-Seq and ecological niche reveal genetic diversity,phylogeny,and geographic distribution of Kadsura interior and its closely related species[J]. Front Plant Sci, 2022,13:857016.DOI: 10.3389/fpls.2022.857016.
[12]	赵儒楠, 褚晓洁, 刘维, 等. 鹅耳枥属树种叶绿体基因组结构及变异分析[J]. 南京林业大学学报(自然科学版), 2021, 45(2):25-34.
	ZHAO R N, CHU X J, LIU W, et al. Structure and variation analyses of chloroplast genomes in Carpinus[J]. J Nanjing For Univ (Nat Sci Ed), 2021, 45(2):25-34.DOI: 10.12302/j.issn.1000-2006.202009007.
[13]	袁钰晨, 谢旭强, 徐立清, 等. 不同年龄阶段胡桃楸天然更新幼树的光合生理特性[J]. 森林工程, 2023, 39(4):29-37.
	YUAN Y C, XIE X Q, XU L Q, et al. Photosynthetic physiological characteristics of naturally regenerated Juglans mandshurica saplings at different ages[J]. For Eng, 2023, 39(4):29-37.
[14]	WANG J, KAN S L, LIAO X Z, et al. Plant organellar genomes:much done,much more to do[J]. Trends Plant Sci, 2024, 29(7):754-769.DOI: 10.1016/j.tplants.2023.12.014.
[15]	邓叶, 李翔, 李平, 等. 黑老虎种质资源与分子生物学研究进展[J]. 湖南生态科学学报, 2024, 11(1):96-104.
	DENG Y, LI X, LI P, et al. Research progress on germplasm resources and molecular biology of Kadsura coccinea[J]. J Hunan Ecol Sci, 2024, 11(1):96-104.DOI: 10.3969/j.issn.2095-7300.2024.01.012.
[16]	LIU L Y, FU Y P, LI Y Q, et al. The complete chloroplast genome sequence of Kadsura ananosma[J]. Mitochondrial DNA B Resour, 2020, 5(1):768-769.DOI: 10.1080/23802359.2020.1715866.
[17]	QIN H Z, DENG L L, SHI Y C. Complete chloroplast genome of Kadsura coccinea (Lem.) A.C.Sm.(Schisandraceae):genome structure and evolution[J]. Mitochondrial DNA B Resour, 2021, 6(3):1222-1223.DOI: 10.1080/23802359.2021.1904798.
[18]	YANG J, WANG X A, GAO M Y, et al. The complete chloroplast genome of ‘black tiger 2’ (Kadsura coccinea (Lem.) A.C.Smith) in southeast of China and phylogenetic relationships[J]. Mitochondrial DNA B Resour, 2019, 5(1):296-297.DOI: 10.1080/23802359.2019.1698328.
[19]	DOYLE J J, DOYLE J L. A rapid DNA isolation procedure for small quantities of fresh leaf tissue[J]. Phytochem Bull, 1987, 19:11-15
[20]	WANG Q H, WANG H, FU Y P, et al. The complete chloroplast genome sequence of Kadsura heteroclita[J]. Mitochondrial DNA B Resour, 2020, 5(3):2197-2198.DOI: 10.1080/23802359.2020.1768963.
[21]	FU Y P, LI Y Q, CHEN W, et al. The complete chloroplast genome sequence of Kadsura interior[J]. Mitochondrial DNA B Resour, 2020, 5(1):515-516.DOI: 10.1080/23802359.2019.1710297.
[22]	JIN L, LIU J J, XIAO T W, et al. Plastome-based phylogeny improves community phylogenetics of subtropical forests in China[J]. Mol Ecol Resour, 2022, 22(1):319-333.DOI: 10.1111/1755-0998.13462.
[23]	WEI X P, LI H J, CHE P, et al. Comparing chloroplast genomes of traditional Chinese herbs Schisandra sphenanthera and S.chinensis[J]. Chin Herb Med, 2020, 12(3):247-256.DOI: 10.1016/j.chmed.2019.09.009.
[24]	GUO H J, LIU J S, LUO L, et al. Complete chloroplast genome sequences of Schisandra chinensis:genome structure,comparative analysis,and phylogenetic relationship of basal angiosperms[J]. Sci China Life Sci, 2017, 60(11):1286-1290.DOI: 10.1007/s11427-017-9098-5.
[25]	JIN J J, YU W B, YANG J B, et al. GetOrganelle:a fast and versatile toolkit for accurate de novo assembly of organelle genomes[J]. Genome Biol, 2020, 21(1):241.DOI: 10.1186/s13059-020-02154-5.
[26]	TILLICH M, LEHWARK P, PELLIZZER T, et al. GeSeq-versatile and accurate annotation of organelle genomes[J]. Nucleic Acids Res, 2017, 45(W1):W6-W11.DOI: 10.1093/nar/gkx391.
[27]	KEARSE M, MOIR R, WILSON A, et al. Geneious basic:an integrated and extendable desktop software platform for the organization and analysis of sequence data[J]. Bioinformatics, 2012, 28(12):1647-1649.DOI: 10.1093/bioinformatics/bts199.
[28]	LOHSE M, DRECHSEL O, BOCK R. OrganellarGenomeDRAW (OGDRAW):a tool for the easy generation of high-quality custom graphical maps of plastid and mitochondrial genomes[J]. Curr Genet, 2007, 52(5/6):267-274.DOI: 10.1007/s00294-007-0161-y.
[29]	KATOH K, STANDLEY D M. MAFFT multiple sequence alignment software version 7:improvements in performance and usability[J]. Mol Biol Evol, 2013, 30(4):772-780.DOI: 10.1093/molbev/mst010.
[30]	ROZAS J, FERRER-MATA A, SÁNCHEZ-DELBARRIO J C, et al. DnaSP 6:DNA sequence polymorphism analysis of large data sets[J]. Mol Biol Evol, 2017, 34(12):3299-3302.DOI: 10.1093/molbev/msx248.
[31]	BEIER S, THIEL T, MÜNCH T, et al. MISA-web:a web server for microsatellite prediction[J]. Bioinformatics, 2017, 33(16):2583-2585.DOI: 10.1093/bioinformatics/btx198.
[32]	STAMATAKIS A. RAxML version 8:a tool for phylogenetic analysis and post-analysis of large phylogenies[J]. Bioinformatics, 2014, 30(9):1312-1313.DOI: 10.1093/bioinformatics/btu033.
[33]	GOULD S B, WALLER R R, MCFADDEN G I. Plastid evolution[J]. Annu Rev Plant Biol, 2008, 59: 491-517. DOI:10.1146/annurev.arplant.59.032607.092915
[34]	杨圆圆, 于世河, 卜鹏图, 等. 不同培育模式下日本落叶松林灌草和土壤养分特征研究[J]. 森林工程, 2023, 39(6):12-25.
	YANG Y Y, YU S H, BU P T, et al. Study on shrub-grass and soil nutrient characteristics of Larix kaempferi forest under different cultivation modes[J]. For Eng, 2023, 39(6):12-25.
[35]	JANSEN R K, RUHLMAN T A. Plastid genomes of seed plants[M]//BOCK R, KNOOP V. Advances in Photosynthesis and Respiration. Dordrecht: Springer Netherlands,2012:103-126.DOI: 10.1007/978-94-007-2920-9_5.
[36]	SAUNDERS R M. Monograph of Kadsura (Schisandraceae)[C]// ANDERSON C. Systematic Botany Monographs. St. Louis: Missouri Botanical Garden Press, 1998.
[37]	刘玉壶. 木兰科[C]//中国科学院中国植物志编辑委员会. 中国植物志. 北京: 科学出版社, 1996.
	LIU Y H. Magnoliaceae[C]//The Agenda of the Chinese Academy. Flora Reipublicae Popularis Sinicae. Beijing: Science Press,1996
[38]	SMITH A C. The families Illiciaceae and Schisandraceae[J]. Sargentia, 1947, 7:1-224.DOI: 10.5962/p.265318.
[39]	毕海燕, 林祁, 刘长江, 等. 南五味子属(五味子科)的种子形态及其分类学意义[J]. 植物分类学报, 2002, 40(6):501-510.
	BI H Y, LIN Q, LIU C J, et al. Seed morphology of Kadsura Juss.(Schisandraceae) in relation to its taxonomic significance[J]. Acta Phytotaxon Sin, 2002, 40(6):501-510.
[40]	LIU Z, HAO G, LUO Y B, et al. Phylogeny and androecial evolution in Schisandraceae,inferred from sequences of nuclear ribosomal DNA ITS and chloroplast DNA trnL-F regions[J]. Int J Plant Sci, 2006, 167(3):539-550.DOI: 10.1086/501476.
[41]	GUO H J, LI X W, QI Y D, et al. Identification of Dian Ji Xue Teng (Kadsura interior) with DNA barcodes[J]. World J Tradit Chin Med, 2017, 3(1):11-15.DOI: 10.15806/j.issn.2311-8571.2016.0017.
[42]	HAO G, CHYE M L, SAUNDERS R M K. A phylogenetic analysis of the Schisandraceae based on morphology and nuclear ribosomal ITS sequences[J]. Bot J Linn Soc, 2001, 135(4):401-411.DOI: 10.1006/bojl.2000.0420.
[43]	CHASE M W, SOLTIS D E, OLMSTEAD R G, et al. Phylogenetics of seed plants:an analysis of nucleotide sequences from the plastid gene rbcL[J]. Ann Mo Bot Gard, 1993, 80(3):528-580.DOI: 10.2307/2399846.
[44]	QIU Y L, LEE J, BERNASCONI-QUADRONI F, et al. The earliest angiosperms:evidence from mitochondrial,plastid and nuclear genomes[J]. Nature, 1999, 402(6760):404-407.DOI: 10.1038/46536.
[45]	SOLTIS D E, SOLTIS P S, CHASE M W, et al. Angiosperm phylogeny inferred from 18S rDNA,rbcL,and atpB sequences[J]. Bot J Linn Soc, 2000, 133(4):381-461.DOI: 10.1111/j.1095-8339.2000.tb01588.x.
[46]	SHAW J, LICKEY E B, BECK J T, et al. The tortoise and the hare Ⅱ:relative utility of 21 noncoding chloroplast DNA sequences for phylogenetic analysis[J]. Am J Bot, 2005, 92(1):142-166.DOI: 10.3732/ajb.92.1.142.
[47]	SHAW J, LICKEY E B, SCHILLING E E, et al. Comparison of whole chloroplast genome sequences to choose noncoding regions for phylogenetic studies in angiosperms:the tortoise and the hare Ⅲ[J]. Am J Bot, 2007, 94(3):275-288.DOI: 10.3732/ajb.94.3.275.
[48]	SHAW J, SHAFER H L, LEONARD O R, et al. Chloroplast DNA sequence utility for the lowest phylogenetic and phylogeographic inferences in angiosperms:the tortoise and the hare Ⅳ[J]. Am J Bot, 2014, 101(11):1987-2004.DOI: 10.3732/ajb.1400398.
[49]	WEISING K. DNA fingerprinting in plants:principles,methods,and applications[M].2nd ed.Boca Raton, FL: Taylor & Francis Group, 2005.
[50]	YAN H F. Phylogeographic structure of Primula obconica (Primulaceae) inferred from chloroplast microsatellites (cpSSRs) markers[J]. Acta Phytotaxon Sin, 2007, 45(4):488-496.DOI: 10.1360/aps06214.

编辑推荐

Metrics

阅读次数

全文

302

HTML			PDF

最新录用	在线预览	正式出版	最新录用	在线预览	正式出版
0	0	8	0	0	294

来源	本网站	其他网站

次数	228	74
比例	75%	25%

摘要

488

最新录用	在线预览	正式出版

0	0	487

来源	本网站	其他网站

次数	33	455
比例	7%	93%

本文评价

www.nldxb.njfu.edu.cn

通信地址：南京市龙蟠路南京林业大学《南京林业大学学报》编辑部
电子邮件：njlyzrb@vip.163.com
联系电话：025-85428247
邮政编码：210037
备案号：苏ICP备-010872

类别category	基因功能gene function	基因gene
光合作用相关 photosynthesis	二磷酸核酮糖羧化酶大亚基	rbcL
	光系统Ⅰ	psaA、psaB、psaC、psaI、psaJ
	光系统Ⅱ	psbA、psbB、psbC、psbD、psbE、psbF、psbH、psbI、psbJ、 psbK、psbL、psbM、psbN、psbT、psbZ
	ATP合酶	atpA、atpB、atpE、atpF^、atpH、atpI*
	细胞色素b/f复合体	petA、petB^、petD^、petG、petL、petN
	NADPH脱氢酶	ndhA^、ndhB^(2)、ndhC、ndhD、ndhE、 ndhF、ndhG、ndhH、ndhI、ndhJ、ndhK
	细胞色素c	ccsA
自身表达相关 self-replication	RNA聚合酶	rpoA、rpoB、rpoC1^、rpoC2*
	核糖体蛋白	rpl2^、rpl14、rpl16、rpl20、rpl22、rpl23、rpl32、rpl33、rpl36、 rps2、rps3、rps4、rps7(2)、rps8、rps11、rps12^(2)、rps14、 rps15、rps16^、rps18、rps19*
	翻译起始因子	infA
RNA相关 RNA-related	核糖体RNAs	rrn4.5(2)、rrn5(2)、rrn16(2)、rrn23(2)
	转运RNAs	trnA-UGC^(2)、trnC-GCA、trnD-GUC、trnE-UUC、trnF-GAA、 trnfM-CAU、trnG-GCC、trnG-UCC^、trnH-GUG、trnI-CAU、trnI-GAU^(2)、 trnK-UUU^、trnL-CAA、trnL-UAA^、trnL-UAG、trnM-CAU、trnN-GUU(2)、 trnP-UGG、trnQ-UUG、trnR-ACG(2)、trnR-UCU、trnS-CGA、trnS-GCU、 trnS-GGA、trnS-UGA、trnT-GGU、trnT-UGU、trnV-GAC(2)、trnV-UAC^、 trnW-CCA、trnY-GUA
	ATP依赖型蛋白酶	clpP^**
其他 others	成熟酶	matK
	乙酰辅酶A羧化酶亚基	accD
	外膜蛋白基因	cemA
	保守的开放阅读框	ycf1(2)、ycf2、ycf3^*、ycf*4

资源植物黑老虎的比较叶绿体基因组学研究

Comparative chloroplast genomics of the important resource plant Kadsura coccinea

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 6

参考文献 50

相关文章 15

编辑推荐

Metrics

本文评价

作者

读者

审稿

编号 No.	物种 species	总长/bp genome size	大单拷贝区长度/bp LSC size	重复区长度/bp IR size	小单拷贝区长度/bp SSC size	GC占比/% GC proportion	总基因数 gene number	蛋白质编码基因数 number of protein- coding genes	rRNA 基因数 number of rRNA genes	tRNA 基因数 number of tRNA genes
HLH-1A	黑老虎	145 617	94 481	16 552	18 032	39.7	125	82	8	35
HLH-2A	黑老虎	145 617	94 481	16 552	18 032	39.7	125	82	8	35
MN480469	黑老虎	145 608	94 457	16 552	18 047	39.7	125	82	8	35
MT934443	黑老虎	145 413	94 511	16 431	18 040	39.7	125	82	8	35
NC_057265	中泰南五味子	145 903	94 757	16 552	18 042	39.7	125	82	8	35
NC_057266	异形南五味子	153 289	85 774	24 657	18 201	39.6	129	84	8	37
NC_050348	异形南五味子	153 201	85 774	24 656	18 115	39.6	129	84	8	37
MW801021	南五味子	153 106	85 593	24 670	18 173	39.6	129	84	8	37

[1]	毛立彦, 李慧敏, 龙凌云, 黄秋伟, 唐毓玮, 於艳萍, 黄歆怡, 檀小辉, 农晓慧, 朱天龙, 陆祖双. 基于SSR分子标记的睡莲遗传多样性分析[J]. 南京林业大学学报（自然科学版）, 2024, 48(5): 57-68.
[2]	闫平玉, 张磊, 王佳兴, 冯可乐, 王浩浩, 张含国. 红松天然种群遗传多样性分析及核心种质构建[J]. 南京林业大学学报（自然科学版）, 2024, 48(5): 69-80.
[3]	马建慧, 陈昕, 耿礼阳, 汤晨茜, 魏雪妍. 蔷薇科花楸属白毛系的系统发生分析[J]. 南京林业大学学报（自然科学版）, 2024, 48(4): 25-36.
[4]	伊贤贵, 李蒙, 王贤荣. 樱亚属分类学研究进展[J]. 南京林业大学学报（自然科学版）, 2024, 48(3): 46-57.
[5]	李涌福, 杨庆华, 陈林, 张敏, 向其柏, 王贤荣, 段一凡. 木犀属内分组关系的分类修订[J]. 南京林业大学学报（自然科学版）, 2024, 48(3): 58-62.
[6]	邢冰冰, 李垚, 毛岭峰. 植物功能性状系统发育保守性的类群和地理分异研究——以中国被子植物最大株高为例[J]. 南京林业大学学报（自然科学版）, 2024, 48(1): 59-66.
[7]	鲁旭东, 董禹然, 李垚, 毛岭峰. 中国亚热带杉木人工林不同林分发育阶段的群落构建机制[J]. 南京林业大学学报（自然科学版）, 2024, 48(1): 67-73.
[8]	王芝懿, 李振芳, 彭婵, 陈英, 张新叶. 基于荧光SSR标记的紫薇遗传多样性分析[J]. 南京林业大学学报（自然科学版）, 2023, 47(2): 61-69.
[9]	王欢利, 严灵君, 黄犀, 王仲伟, 汤诗杰. 南京椴群体遗传多样性和遗传结构分析[J]. 南京林业大学学报（自然科学版）, 2023, 47(1): 145-153.
[10]	冯一宁, 李因刚, 祁铭, 周鹏燕, 周琦, 董乐, 徐立安. 基于SSR标记的福建省闽楠代表性群体遗传多样性分析[J]. 南京林业大学学报（自然科学版）, 2022, 46(4): 102-108.
[11]	吕锋, 解孝满, 韩彪, 鲁仪增, 王磊, 董昕, 王艳, 陆璐, 刘莉, 宗绍宁, 李文清. 基于SSR标记的麻栎天然群体遗传多样性分析[J]. 南京林业大学学报（自然科学版）, 2022, 46(3): 109-116.
[12]	葛大朋, 任媛, 赵俊, 王玉婷, 刘学庆, 苑兆和. 西藏石榴野生群体的SSR遗传多样性分析[J]. 南京林业大学学报（自然科学版）, 2022, 46(3): 127-133.
[13]	何旭东, 郑纪伟, 教忠意, 窦全琴, 黄利斌. 基于SLAF-seq技术的舒玛栎群体遗传多样性与遗传结构分析[J]. 南京林业大学学报（自然科学版）, 2022, 46(2): 81-87.
[14]	高景斌, 徐六一, 叶建仁. 马尾松松材线虫病抗性无性系的筛选和遗传多样性分析[J]. 南京林业大学学报（自然科学版）, 2021, 45(5): 109-118.
[15]	陈兴彬, 徐海宁, 肖复明, 孙世武, 娄永峰, 邹元熹, 徐小强. 陈山红心杉1.5代种子园遗传多样性和子代父本分析[J]. 南京林业大学学报（自然科学版）, 2021, 45(3): 87-92.