南京林业大学学报(自然科学版) ›› 2025, Vol. 49 ›› Issue (3): 52-62.doi: 10.12302/j.issn.1000-2006.202408010
• 专题报道Ⅱ:乡村全面振兴视域下蓝莓优新品种培育研究 • 上一篇 下一篇
赵慧芳1(), 赵俸艺1, 刘洪霞1, 吴文龙1, 吴雅琼1,*(
), 李维林2,*(
)
收稿日期:
2024-08-12
接受日期:
2024-09-27
出版日期:
2025-05-30
发布日期:
2025-05-27
通讯作者:
*李维林(wlli@njfu.cn),研究员,负责试验方案设计;作者简介:
赵慧芳(nuzhenzi-1984@163.com),助理研究员。
基金资助:
ZHAO Huifang1(), ZHAO Fengyi1, LIU Hongxia1, WU Wenlong1, WU Yaqiong1,*(
), LI Weilin2,*(
)
Received:
2024-08-12
Accepted:
2024-09-27
Online:
2025-05-30
Published:
2025-05-27
摘要:
【目的】综合评价南京地区46个蓝莓品种的果实特性,为蓝莓良种筛选和育种提供参考信息。【方法】测定蓝莓果实外观和内在品质指标,考察感官特点并评分,通过相关性、主成分和聚类分析对46个蓝莓品种进行分类评定。【结果】从3种类型来看,北高丛蓝莓果实最大,硬度和酸度最高,感官评分最高;南高丛蓝莓果实偏小,硬度最低,甜度最高,总多酚含量最高,感官评分最低;兔眼蓝莓的果实较大,果皮占比最高,总花色苷含量最高。外观指标中去果粉色度a*的变异系数最高(2 926.91%),其次为原果色度(370.96%);品质指标中固酸比的变异系数最高(42.77%),其次为果实总花色苷含量(39.78%);感官评分的变异系数最小(低于10%)。相关性分析结果表明,果实越大果皮占比越低,总多酚含量越低。总酸含量是果实酸甜度的决定性指标。外观和综合评分与代表果实大小的单果质量、横径和纵径呈极显著正相关,可见大果是感官评定时的一个首选指标。聚类分析将46个品种分为3类,其中第Ⅰ类果实大,硬度高,酸度高;第Ⅱ类果实较大,甜度高;第Ⅲ类果实较小,果皮占比高,属于高花色苷和多酚含量的加工类型。以主成分分析将11个外观和品质指标降维为4个主成分,累计贡献率为75.217%。感官评定发现,46个蓝莓品种的果实除大小、酸甜度和营养成分含量高低的差异之外,还有果粉厚度、口感以及果香味的差异,并通过感官评定确定了7个大果品种‘天后’、‘新千年’、‘珠宝’、‘钱德勒’、‘布里吉塔’、‘奥罗拉’和‘巨蓝’,3个甜度较高的品种‘安娜’、‘甜心’和‘灿烂’,4个口感极佳的品种‘阳光’、‘玉蓝’、‘布里吉塔’和‘贝姬蓝’,3个果香浓郁的品种‘玉蓝’、‘甜心’和‘红粉佳人’。综合评定发现,感官评定与主成分及聚类分析的结果基本一致。【结论】46个蓝莓品种中,‘巨蓝’、‘钱德勒’、‘甜心’、‘布里吉塔’、‘新千年’、‘贝姬蓝’、‘天后’、‘灿烂’、‘玉蓝’、‘红粉佳人’等10个品种果实的鲜食品质较好(大果、高甜度、口感脆硬和果香浓郁),推荐用于鲜食; ‘园蓝’、‘中植4号’、‘寨选4号’、‘巴尔德温’和‘森吐里昂’等5个品种果实的营养品质较好(高花色苷、多酚含量和高果皮占比),推荐用于加工。这些品种也可以作为相应育种目标的亲本材料。
中图分类号:
赵慧芳,赵俸艺,刘洪霞,等. 蓝莓46个品种的果实特性分析及综合评价[J]. 南京林业大学学报(自然科学版), 2025, 49(3): 52-62.
ZHAO Huifang, ZHAO Fengyi, LIU Hongxia, WU Wenlong, WU Yaqiong, LI Weilin. A comprehensive evaluation of fruit characteristics of 46 blueberry cultivars[J].Journal of Nanjing Forestry University (Natural Science Edition), 2025, 49(3): 52-62.DOI: 10.12302/j.issn.1000-2006.202408010.
表1
46个蓝莓品种的外观、品质指标和感官评分的描述统计"
类别 type | 指标 index | 南高丛SHB | 北高丛NHB | 兔眼rabbiteye | 整体overall | ||||
---|---|---|---|---|---|---|---|---|---|
平均值 mean | 变异 系数/ % CV | 平均值 mean | 变异 系数/ % CV | 平均值 mean | 变异 系数/ % CV | 平均值 mean | 变异 系数/ % CV | ||
外观 指标 appearance charac- teristics | 单果质量/g FW | 1.98±0.77 | 39.00 | 2.78±0.82 | 29.40 | 2.18±0.54 | 24.81 | 2.20±0.74 | 33.65 |
横径/mm TD | 15.56±2.18 | 14.00 | 17.82±2.15 | 12.08 | 15.98±1.50 | 9.41 | 16.12±2.06 | 12.78 | |
纵径/mm VD | 12.39±1.68 | 13.55 | 13.34±1.44 | 10.77 | 12.89±0.90 | 7.00 | 12.75±1.40 | 10.94 | |
果形指数FS | 0.80±0.06 | 7.42 | 0.75±0.04 | 5.94 | 0.81±0.04 | 5.16 | 0.80±0.05 | 6.76 | |
硬度/(kg·cm-2) HD | 2.58±0.40 | 15.49 | 2.88±0.47 | 16.36 | 2.80±0.44 | 15.81 | 2.72±0.44 | 16.10 | |
L* | 37.89±3.49 | 9.21 | 38.09±2.22 | 5.82 | 42.89±4.59 | 10.70 | 39.88±4.45 | 11.16 | |
a* | -1.37±0.32 | 23.18 | -1.47±0.16 | 10.56 | 0.05±4.87 | 8 943.68 | -0.83±3.08 | 370.96 | |
b* | -8.30±1.26 | 15.23 | -8.18±0.45 | 5.51 | 8.20±2.05 | 25.02 | -8.24±1.52 | 18.40 | |
L*-去果粉L*-wp | 25.03±1.48 | 5.93 | 25.21±1.69 | 6.70 | 27.27±2.09 | 7.67 | 25.94±2.05 | 7.90 | |
a*-去果粉a*-wp | -0.42±0.26 | 61.96 | -0.44±0.15 | 34.40 | 0.44±4.05 | 917.93 | -0.09±2.53 | 2 926.91 | |
b*-去果粉b*-wp | -3.55±0.67 | 18.91 | -3.39±0.35 | 10.43 | 3.49±1.08 | 30.84 | -3.50±0.81 | 23.05 | |
品质 指标 nutritional charac- teristics | 可溶性固形物质量分数/% SSC | 12.04±1.29 | 10.70 | 11.22±1.19 | 10.57 | 11.63±1.34 | 11.49 | 11.74±1.3 | 11.07 |
总酸质量分数/% TAC | 0.46±0.21 | 44.50 | 0.52±0.18 | 35.28 | 0.50±0.13 | 25.53 | 0.49±0.17 | 35.42 | |
固酸比SAR | 31.43±13.93 | 44.32 | 23.46±6.22 | 26.51 | 25.32±10.33 | 40.79 | 27.65±11.83 | 42.77 | |
果皮占比/% PP | 7.39±2.49 | 33.77 | 5.74±1.52 | 26.55 | 7.50±1.88 | 25.11 | 7.14±2.18 | 30.53 | |
总花色苷(果实)质量分数/(mg·g-1) ACY | 1.35±0.52 | 38.62 | 1.33±0.32 | 24.12 | 1.49±0.68 | 45.63 | 1.40±0.56 | 39.78 | |
总多酚(果实)质量分数/(mg·g-1) TPC | 2.77±0.69 | 24.98 | 2.29±0.30 | 13.11 | 2.48±0.96 | 38.70 | 2.57±0.77 | 30.07 | |
总花色苷(果皮)质量分数/(mg·g-1) PCY | 20.52±8.54 | 41.59 | 20.18±5.68 | 28.15 | 20.81±8.38 | 40.25 | 20.58±7.90 | 38.37 | |
总多酚(果皮)质量分数/(mg·g-1) PPC | 30.94±10.6 | 34.25 | 30.04±8.95 | 29.78 | 27.54±10.12 | 36.74 | 29.45±10.05 | 34.13 | |
感官 评分 sensory score | 外观AP | 6.91±0.54 | 7.84 | 7.60±0.53 | 6.91 | 7.45±0.65 | 8.73 | 7.24±0.65 | 8.91 |
酸甜度SS | 6.62±0.50 | 7.42 | 6.83±0.31 | 4.47 | 6.72±0.55 | 8.20 | 6.69±0.49 | 7.34 | |
口感TS | 6.75±0.40 | 6.12 | 7.13±0.24 | 3.37 | 6.88±0.58 | 8.42 | 6.87±0.47 | 6.85 | |
综合CH | 6.73±0.58 | 8.50 | 7.24±0.33 | 4.56 | 7.12±0.62 | 8.76 | 6.97±0.59 | 8.53 |
表2
不同组别蓝莓果实外观和品质指标的均值和变异系数"
指标 indices | 最小值min | 最大值max | 平均值mean | 变异系数/% CV | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Ⅰ | Ⅱ | Ⅲ | Ⅰ | Ⅱ | Ⅲ | Ⅰ | Ⅱ | Ⅲ | Ⅰ | Ⅱ | Ⅲ | |
单果质量/g FW | 1.70 | 1.20 | 0.89 | 3.88 | 3.84 | 2.50 | 2.55±0.59 | 2.53±0.91 | 1.67±0.41 | 23.22 | 35.77 | 24.87 |
横径/mm TD | 15.23 | 13.13 | 12.05 | 20.49 | 19.99 | 16.85 | 17.29±1.58 | 16.83±2.28 | 14.55±1.28 | 9.11 | 13.55 | 8.77 |
纵径/mm VD | 11.75 | 10.86 | 9.76 | 15.11 | 17.48 | 13.37 | 13.12±0.81 | 13.71±1.96 | 11.86±0.97 | 6.17 | 14.28 | 8.17 |
果形指数FS | 0.67 | 0.74 | 0.76 | 0.83 | 0.92 | 0.92 | 0.76±0.04 | 0.82±0.06 | 0.82±0.04 | 5.56 | 7.73 | 5.18 |
硬度/(kg·cm-2) HD | 1.94 | 1.88 | 2.05 | 3.70 | 3.44 | 3.03 | 2.89±0.51 | 2.64±0.46 | 2.59±0.28 | 17.80 | 17.36 | 10.83 |
可溶性固形物质量分数/% SSC | 10.48 | 8.31 | 9.84 | 14.98 | 13.70 | 13.47 | 11.85±1.16 | 11.12±1.69 | 11.96±1.15 | 21.16 | 37.64 | 22.29 |
总酸质量分数/% TAC | 0.36 | 0.19 | 0.36 | 0.85 | 0.39 | 13.94 | 0.56±0.14 | 0.25±0.06 | 0.54±0.13 | 9.77 | 15.20 | 9.61 |
固酸比SAR | 13.01 | 26.05 | 16.66 | 30.89 | 64.13 | 0.79 | 22.10±5.14 | 45.77±11.11 | 23.13±5.58 | 24.58 | 23.85 | 23.59 |
果皮占比/% PP | 4.78 | 3.53 | 5.48 | 9.30 | 10.51 | 36.42 | 6.44±1.36 | 5.57±2.09 | 8.72±1.94 | 23.24 | 24.28 | 24.12 |
总花色苷(果实)质量分数/(mg·g-1) ACY | 0.05 | 0.81 | 0.70 | 1.73 | 1.86 | 2.81 | 1.12±0.41 | 1.32±0.41 | 1.74±0.60 | 37.02 | 31.04 | 34.27 |
总多酚(果实)质量分数/(mg·g-1) TPC | 0.87 | 1.27 | 1.25 | 2.18 | 1.98 | 3.08 | 1.45±0.33 | 1.54±0.28 | 2.08±0.57 | 22.64 | 18.27 | 27.35 |
表3
各主成分的特征向量、特征值和贡献率"
指标index | PC1 | PC2 | PC3 | PC4 |
---|---|---|---|---|
单果质量FW | 0.483 | 0.126 | -0.133 | 0.149 |
横径HD | 0.480 | 0.172 | -0.075 | 0.176 |
纵径VD | 0.440 | -0.058 | -0.239 | 0.020 |
果形指数FS | -0.187 | -0.384 | -0.253 | -0.295 |
硬度HD | 0.080 | 0.252 | 0.513 | 0.112 |
果皮占比PP | -0.329 | 0.185 | 0.003 | -0.133 |
可溶性固形物含量SSC | -0.147 | 0.192 | 0.532 | 0.128 |
总酸含量/ TAC | -0.160 | 0.591 | -0.212 | -0.118 |
固酸比SAR | 0.104 | -0.559 | 0.387 | 0.087 |
总花色苷(果实)含量ACY | -0.208 | -0.056 | -0.126 | 0.804 |
总多酚(果实)含量TPC | -0.303 | -0.068 | -0.315 | 0.380 |
特征值eigenvalue | 3.760 | 2.137 | 1.366 | 1.010 |
贡献率/% contribution rate | 34.185 | 19.430 | 12.418 | 9.185 |
累计贡献率/% cumulative contribution rate | 34.185 | 53.615 | 66.032 | 75.217 |
表4
参试46个蓝莓品种鲜果的感官描述、外观评分及主成分得分排名"
类型 type | 品种 cultivar | 感官描述 sensory description | 外观评分 appearance score | 酸甜度评分 sour and sweet score | 口感评分 taste score | 综合评分 comprehensive score | 主成分 综合得分 PCA score | 主成分 排名 PCA ranking |
---|---|---|---|---|---|---|---|---|
南高丛 SHB | ‘夏普蓝’ | 多籽 | 6.73±0.18 ijklmn | 7.02±0.18 abcd | 7.05±0.17 abcde | 6.96±0.16 bcdefghij | 0.18 | 15 |
‘奥尼尔’ | 果香浓 | 6.82±0.22 fghigklmn | 6.70±0.26 abcde | 6.85±0.27 abcdef | 7.36±0.26 abcdef | 0.25 | 13 | |
‘阳光’ | 口感脆,少籽 | 7.01±0.14 efghijkl | 7.08±0.19 abcd | 7.20±0.19 abcde | 7.22±0.17 abcdefg | -0.35 | 33 | |
‘玉蓝’ | 口感脆、少籽,果香浓 | 7.42±0.16 abcdefghijk | 7.15±0.20 abc | 7.58±0.17 a | 7.56±0.17 abcd | 0.16 | 16 | |
‘蓝雨’ | 圆球形 | 6.28±0.17 lnm | 6.09±0.19 cde | 6.65±0.15 abcdef | 6.36±0.16 fghijk | -0.76 | 42 | |
‘安娜’ | 甜度高,果香浓 | 7.35±0.22 abcdefghijkl | 7.53±0.26 ab | 7.59±0.23 ab | 7.89±0.17 abc | 0.05 | 20 | |
‘比洛克西’ | — | 6.85±0.26 fghijklmn | 5.54±0.40 e | 6.11±0.38 def | 5.90±0.31 ijk | 0.02 | 23 | |
‘薄雾’ | — | 6.33±0.31 klmn | 6.26±0.32 bcde | 6.29±0.29 bcdef | 5.98±0.29 hijk | -0.80 | 43 | |
‘南方之星’ | — | 7.44±0.29 abcdefghijkl | 6.71±0.33 abcde | 6.77±0.27 abcdef | 7.00±0.29 abcdefghijk | 0.09 | 19 | |
‘顶级’ | 果香浓 | 6.83±0.20 defghijklmn | 6.56±0.32 abcde | 6.87±0.25 abcdef | 6.84±0.22 abcdefghijk | -0.06 | 26 | |
‘天后’ | 大果 | 7.58±0.27 abcdefghijk | 6.65±0.30 abcde | 6.60±0.34 abcdef | 6.91±0.27 abcdefghijk | 0.68 | 7 | |
‘新千年’ | 大果 | 8.00±0.16 abc | 6.63±0.21 abcde | 6.62±0.19 abcdef | 6.92±0.18 cdefghij | 0.87 | 5 | |
‘绿宝石’ | 圆球形 | 6.41±0.20 klmn | 5.82±0.24 de | 6.27±0.22 cdef | 5.68±0.24 k | -0.84 | 44 | |
‘珠宝’ | 大果,圆球形 | 7.65±0.15 abcdefghi | 7.00±0.22 abcd | 6.95±0.20 abcde | 7.23±0.18 abcdefg | 0.04 | 22 | |
‘中植1号’ | 多籽 | 6.01±0.15 n | 6.04±0.23 de | 6.26±0.21 ef | 5.98±0.18 jk | -0.56 | 39 | |
‘寨选4号’ | 多籽 | 6.91±0.20 ghijklmn | 6.77±0.19 abcde | 6.90±0.18 abcdef | 6.93±0.18 bcdefghij | -0.27 | 30 | |
‘中植2号’ | — | 6.05±0.16 mn | 6.44±0.19 bcde | 6.57±0.18 bcdef | 6.33±0.16 ghijk | -1.01 | 46 | |
‘寨选7号’ | — | 6.65±0.17 jklmn | 6.56±0.18 abcde | 6.46±0.19 bcdef | 6.37±0.17 fghijk | -0.68 | 41 | |
‘中植3号’ | — | 7.02±0.24 defghijkl | 6.55±0.25 abcde | 6.82±0.22 abcdef | 6.71±0.22 cdefghijk | -0.54 | 38 | |
‘中植4号’ | 口感脆 | 6.86±0.22 hijklmn | 6.60±0.27 abcde | 6.70±0.28 abcdef | 6.63±0.26 defghijk | 0.04 | 21 | |
北高丛 NHB | ‘钱德勒’ | 大果 | 8.20±0.21 ab | 6.98±0.22 abcde | 7.32±0.19 abcde | 7.50±0.19 abcdefg | 1.12 | 2 |
‘莱克西’ | — | 7.42±0.18 abcdefghijk | 6.51±0.22 abcde | 6.96±0.19 abcde | 7.00±0.19 abcdefghi | 0.20 | 14 | |
‘达柔’ | 果香浓 | 7.36±0.18 abcdefghijk | 6.78±0.20 abcde | 7.26±0.18 abcd | 7.05±0.16 abcdefghi | 0.11 | 18 | |
‘泽西’ | 果粉厚,果香浓 | 6.64±0.16 jklmn | 7.02±0.18 abcd | 7.05±0.17 abcde | 6.97±0.17 abcdefghij | -0.51 | 37 | |
‘布里吉塔’ | 大果,果粉厚,口感脆,少籽 | 8.17±0.15 abcd | 7.06±0.23 abcd | 7.50±0.18 abc | 7.80±0.14 abcd | 0.95 | 4 | |
‘甜心’ | 甜度高,口感脆,果香浓 | 7.90±0.14 abcdefgh | 7.23±0.17 abcd | 7.26±0.17 abcde | 7.56±0.20 abcdef | 1.04 | 3 | |
‘瑞贝尔’ | — | 7.80±0.15 abcdefg | 6.75±0.19 abcde | 6.80±0.16 abcdef | 7.01±0.16 abcdefghi | 0.52 | 9 | |
‘奥罗拉’ | 大果,口感脆 | 7.29±0.18 abcdefghijkl | 6.31±0.20 abcde | 6.90±0.19 abcdef | 7.00±0.17 abcdefghijk | 0.56 | 8 | |
兔眼 rabbiteye | ‘粉蓝’ | 果粉厚 | 7.17±0.22 abcdefghijklmn | 6.52±0.28 abcde | 6.75±0.24 abcdef | 6.88±0.23 abcdefghijk | -0.44 | 36 |
‘园蓝’ | 果粉厚 | 7.17±0.29 abcdefghijklmn | 7.33±0.35 abcd | 7.21±0.31 abcde | 7.49±0.28 abcdef | -0.05 | 25 | |
‘森吐里昂’ | — | 6.87±0.21 cdefghijklmn | 6.13±0.24 bcde | 6.23±0.25 bcdef | 6.27±0.25 fghijk | -0.57 | 40 | |
‘梯芙蓝’ | 果粉厚 | 6.90±0.27 bcdefghijklmn | 6.23±0.29 bcde | 6.54±0.26 abcdef | 6.58±0.24 cdefghijk | -0.10 | 27 | |
‘灿烂’ | 果粉厚,甜度高 | 8.25±0.23 ab | 7.81±0.22 a | 7.81±0.24 a | 8.14±0.25 a | 0.47 | 10 | |
‘顶峰’ | 果粉厚 | 6.87±0.32 cdefghijklmn | 6.15±0.29 bcde | 5.71±0.28 f | 6.22±0.27 fghijk | 0.42 | 11 | |
‘杰兔’ | 果粉厚,多籽 | 7.88±0.30 abcdefghi | 6.35±0.29 abcde | 6.31±0.26 bcdef | 6.87±0.27 abcdefghijk | 0.42 | 12 | |
‘乌达德’ | 果粉厚 | 7.19±0.25 abcdefghijklm | 5.81±0.29 de | 6.44±0.20 abcdef | 6.48±0.18 defghijk | -0.31 | 31 | |
‘精华’ | 果粉厚 | 7.21±0.25 abcdefghijklm | 7.02±0.24 abcde | 7.17±0.21 abcdef | 7.35±0.20 abcdefgh | -0.36 | 34 | |
‘巴尔德温’ | — | 7.98±0.20 abcdefgh | 6.84±0.26 abcde | 7.10±0.22 abcdef | 7.39±0.19 abcdefg | -0.42 | 35 | |
‘巨蓝’ | 大果 | 8.08±0.30 abcdef | 7.08±0.29 abcd | 7.31±0.28 abcde | 7.64±0.28 abcde | 1.23 | 1 | |
‘布蓝特蓝’ | 果粉厚 | 6.87±0.21 cdefghijklmn | 6.42±0.21 abcde | 6.42±0.22 abcdef | 6.58±0.19 cdefghijk | -0.22 | 29 | |
‘贝姬蓝’ | 果粉厚,口感脆,果香浓 | 8.15±0.21 abcde | 7.63±0.22 ab | 7.79±0.20 a | 8.14±0.22 ab | 0.72 | 6 | |
‘沃农’ | 果粉厚 | 8.52±0.22 a | 7.29±0.23 abcd | 7.58±0.21 abc | 7.92±0.21 abc | 0.12 | 17 | |
‘昂丝萝’ | 果粉厚 | 7.08±0.21 bcdefghijklmn | 6.77±0.26 abcde | 6.98±0.20 abcdef | 7.10±0.17 abcdefghijk | -0.33 | 32 | |
‘乡铃’ | 圆球形,多籽 | 6.10±0.19 lmn | 6.28±0.24 abcde | 6.29±0.23 bcdef | 6.36±0.19 efghijk | -0.86 | 45 | |
‘博尼塔’ | 果粉厚,果香浓 | 7.79±0.26 abcdefghij | 6.62±0.24 abcde | 7.02±0.21 abcdef | 7.22±0.22 abcdefghijk | -0.05 | 24 | |
‘红粉佳人’ | 无果粉,果香浓 | 8.05±0.25 abcdef | 6.70±0.25 abcde | 7.17±0.24 abcde | 7.44±0.27 abcdefg | -0.18 | 28 |
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