流苏树叶片代谢物地理变异及其综合评价

牛牧歌; 刘来硕; 王锦楠; 陈荣; 武玉柱; 郭历阳; 李际红

doi:10.12302/j.issn.1000-2006.202409003

流苏树叶片代谢物地理变异及其综合评价

牛牧歌, 刘来硕, 王锦楠, 陈荣, 武玉柱, 郭历阳, 李际红

南京林业大学学报（自然科学版） ›› 2025, Vol. 49 ›› Issue (5) : 123-130.

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南京林业大学学报（自然科学版） ›› 2025, Vol. 49 ›› Issue (5) : 123-130. DOI: 10.12302/j.issn.1000-2006.202409003

研究论文

流苏树叶片代谢物地理变异及其综合评价

牛牧歌 ¹^,³ ,
刘来硕 ¹ ,
王锦楠 ¹ ,
陈荣 ² ,
武玉柱 ¹ ,
郭历阳 ¹ ,
李际红 ¹^,^*

作者信息 +

Geographical variation of metabolites in Chionanthus retusus leaves and its comprehensive evaluation

NIU Muge ¹^,³ ,
LIU Laishuo ¹ ,
WANG Jinnan ¹ ,
CHEN Rong ² ,
WU Yuzhu ¹ ,
GUO Liyang ¹ ,
LI Jihong ¹^,^*

Author information +

文章历史 +

摘要

【目的】探究流苏树(Chionanthus retusus)叶片代谢物的地理变异,筛选代谢物含量优良的种源,为流苏树茶用品种选育和流苏树种质资源的保护、开发提供理论基础。【方法】以8个省份15个种源的150份流苏树单株叶片为材料,对其茶多酚、可溶性糖、谷氨酸、抗坏血酸、类黄酮和花青素含量指标进行研究,通过方差分析、变异分析、相关性分析、聚类分析、主成分分析及综合评价,筛选代谢物含量优良的流苏树种源。【结果】种源间和种源内流苏树叶片的6种代谢物指标的差异均达到极显著水平(P<0.001),流苏树种质资源性状变异丰富;代谢物相关性结果显示,流苏树叶片中谷氨酸与可溶性糖含量呈极显著正相关,茶多酚和抗坏血酸、谷氨酸的含量呈显著正相关。种源地气候因子与代谢物相关性结果显示,等温性与谷氨酸含量间、年降水量与花青素含量间存在显著的正相关关系;聚类分析将15个流苏树种源划分为3个类群,并未按照地理位置分布进行聚类分析。通过综合评价筛选出4个优良种源,即河南南召、湖北宜昌、山东临沂和河北承德种源。【结论】15个种源流苏树叶片6类代谢物在种源内与种源外均存在显著差异,筛选出的河南南召、湖北宜昌、山东临沂和河北承德4个优良种源遗传变异丰富,代谢物含量优良。

Abstract

【Objective】To investigate the geographical variation of leaf metabolites in Chionanthus retusus and select provenances with superior metabolite content, this study provides a foundation for breeding tea-oriented varieties of Chionanthus retusus, as well as the conservation and utilization of its germplasm resources. 【Method】Using leaf samples from 150 individual Chionanthus retusus trees representing 15 provenances across eight provinces, this study analyzed the contents of tea polyphenols, soluble sugars, glutamig acid, ascorbic acid, flavonoids, and anthocyanins. Through variance analysis, coefficient of variation assessment, correlation analysis, cluster analysis, principal component analysis (PCA), and comprehensive evaluation, we identified provenances with superior metabolite profiles. 【Result】Both inter-provenance and intra-provenance differences reached highly significant levels (P < 0.001), indicating rich trait variation in Chionanthus retusus germplasm resources. Metabolite correlation analysis revealed a highly significant positive correlation between glutamic acid and soluble sugars, as well as significant positive correlations between tea polyphenols and ascorbic acid, and between tea polyphenols and glutamic acid. Climate-metabolite correlation analysis demonstrated significant positive relationships between isothermality (bio-climatic factor) and glutamic acid, and between annual precipitation and anthocyanins. Cluster analysis grouped the 15 Chionanthus retusus provenances into three distinct clusters, which did not align with their geographical distribution. Comprehensive evaluation finally identified four superior provenances: Nanzhao (Henan), Yichang (Hubei), Linyi (Shandong), and Chengde (Hebei). 【Conclusion】Significant differences were detected in six classes of leaf metabolites both within and among 15 Chionanthus retusus provenances. Through comprehensive evaluation, four superior provenances were identified: Nanzhao (Henan), Yichang (Hubei), Linyi (Shandong), and Chengde (Hebei).

导出引用

牛牧歌, 刘来硕, 王锦楠, 等. 流苏树叶片代谢物地理变异及其综合评价[J]. 南京林业大学学报（自然科学版）. 2025, 49(5): 123-130 https://doi.org/10.12302/j.issn.1000-2006.202409003

NIU Muge, LIU Laishuo, WANG Jinnan, et al. Geographical variation of metabolites in Chionanthus retusus leaves and its comprehensive evaluation[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2025, 49(5): 123-130 https://doi.org/10.12302/j.issn.1000-2006.202409003

中图分类号： S791

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