青海省甘蒙柽柳根际土壤细菌群落特征及功能分析

周妍旭, 李强, 刘蕾, 王祥福, 孙启武, 杨芾, 厚凌宇, 刘勇, 吴梁厚, 周宏权, 王宝荣

南京林业大学学报（自然科学版） ›› 2026, Vol. 50 ›› Issue (2) : 236-244.

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南京林业大学学报（自然科学版）
PDF(4333 KB)
南京林业大学学报（自然科学版） ›› 2026, Vol. 50 ›› Issue (2) : 236-244. DOI: 10.12302/j.issn.1000-2006.202409015
研究论文

青海省甘蒙柽柳根际土壤细菌群落特征及功能分析

作者信息 +

Analysis of rhizosphere soil bacterial community characteristics and functional bacterial excavation of Tamarix austromongolica in Qinghai Province

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文章历史 +

摘要

【目的】对青海省甘蒙柽柳(Tamarix austromongolica)根际土壤细菌类群多样性、群落组成和促生功能进行系统分析,为青海省甘蒙柽柳树种保护及其原生微生物资源开发提供基础。【方法】以青海省海南藏族自治州然果村(RG)、上鹿圈村(SL)、上加吾沟村(SJ)等3个保护区的甘蒙柽柳根际土壤为研究对象,基于高通量测序技术分析甘蒙柽柳根际土壤细菌多样性、群落组成,采用传统稀释培养方法分离根际土壤细菌,并利用功能平板和特异性颜色变化进行功能细菌筛选。【结果】然果村(RG)与上鹿圈村(SL)、上加吾沟村(SJ)甘蒙柽柳根际土壤细菌Alpha和Beta多样性存在显著差异,但3个保护区的细菌优势菌门均为变形菌门(Proteobacteria)、酸杆菌门(Acidobacteriota)和放线菌门(Actinomycetota);假单胞菌属(Pseudomona)、未分类细菌和unclassified_Gemmatimonadaceae分别为不同保护区的生物标记类群;利用共现网络筛选获得32个核心类群;从不同保护区的甘蒙柽柳根际土壤中共分离获得55株细菌,其中2株为潜在新种;11株属于核心类群链霉菌属(Streptomyces)、鞘氨醇单胞菌属(Sphingomonas)、溶杆菌属(Lysobacter)和德沃斯氏菌属(Devosia);有27株具有潜在促生功能,其中12株具有溶解无机磷能力、8株具有矿化有机磷能力、8株具有解钾能力,15株具有分泌IAA能力,分离自RG的菌株Oceanbacillus sojae RGGN1同时具有4种功能。【结论】不同保护区甘蒙柽柳根际土壤细菌多样性和群落组成存在一定差异,利用可培养方法明确11株核心类群,获得2个潜在新种和27株具有潜在促生功能的菌株,丰富地域特色和自主知识产权的优质功能菌株资源库。

Abstract

【Objective】This study systematically analyzed the diversity, community composition, and growth-promoting functions of rhizosphere soil bacteria in Tamarix austromongolica from Qinghai Province. The research aims to provide a basis for the conservation of this tree species and the development of its indigenous microbial resources.【Method】Using the rhizosphere soil of T. austromongolica in three conservation areas, Ranguo Village (RG), Shanglujuan Village (SL), and Shangjiawugou Village (SJ) in Hainan Zang Autonomous Prefecture, Qinghai Province as the research object, the bacterial diversity and community composition of the rhizosphere soil of T. austromongolica were analyzed based on high-throughput sequencing, rhizosphere soil bacteria were isolated using the traditional dilution culture method, and functional evaluation was performed with functional plates and specific color changes.【Result】There are significant differences in the Alpha and Beta diversity of rhizosphere soil bacteria in T. austromongolica from Ranguo Village (RG) compared to Shanglujuan Village (SL) and Shangjiawugou Village (SJ). However, the dominant bacterial phyla across all three conservation areas are Proteobacteria, Acidobacteriota, and Actinomycetota. Pseudomonas, unclassified_bacteria, and unclassified_Gemmatimonadaceae are the biomarker for the different conservation areas, respectively. A total of 55 strains are isolated from the rhizosphere soil of T. austromongolica across different conservation areas, among which two strains are potential new species, and 11 strains belong to the core genera of Streptomyces, Sphingomonas, Lysobacter and Devosia. Twenty-seven strains exhibit potential growth-promoting functions, with 12 strains processing inorganic phosphorus-dissolving ability, eight strains demonstrating organic phosphorus-mineralizing ability, eight strains showing potassium-dissolving ability, and 15 strains capable of IAA-producing ability. Among there, the strain Oceanbacillus sojae RGGN1 isolated from RG possessed the functions of inorganic phosphorus-dissolving, organic phosphorus-mineralizing, potassium dissolving and IAA-producing ability. Simultaneously possess three of these functions.【Conclusion】There are certain differences in the rhizosphere soil bacterial diversity and community composition of T. austromongolica in different conservation areas. Using culturable methods, 11 core groups were identified, and two potential new species along with 27 strains exhibiting potential growth-promoting functions were obtained. This work enriches the resource base of high-quality functional bacterial strains with regional characteristics and independent intellectual property rights.

关键词

甘蒙柽柳 / 植物根际促生菌 / 群落结构 / 高通量测序 / 分离培养 / 功能菌评价 / 青海

Key words

Tamarix austromongolica / plant growth-promoting rhizobacteria / community structure / high-throughput sequencing / isolation and cultivation / function bacterial assessment / Qinghai

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周妍旭, 李强, 刘蕾, 等. 青海省甘蒙柽柳根际土壤细菌群落特征及功能分析[J]. 南京林业大学学报（自然科学版）. 2026, 50(2): 236-244 https://doi.org/10.12302/j.issn.1000-2006.202409015
ZHOU Yanxu, LI Qiang, LIU Lei, et al. Analysis of rhizosphere soil bacterial community characteristics and functional bacterial excavation of Tamarix austromongolica in Qinghai Province[J]. Journal of Nanjing Forestry University (Natural Sciences Edition）. 2026, 50(2): 236-244 https://doi.org/10.12302/j.issn.1000-2006.202409015
中图分类号： S718   

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基金

黄河羊曲水电站工程库区甘蒙柽柳保护科研项目
科技基础资源调查专项(2021FY100803)

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