外源脱落酸与低温预处理结合对红松体胚成熟的影响

史玉洁; 任悦; 汤铄然; 杨玲

doi:10.12302/j.issn.1000-2006.202409034

史玉洁, 任悦, 汤铄然, 杨玲

南京林业大学学报（自然科学版） ›› 2025, Vol. 49 ›› Issue (4) : 71-78.

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南京林业大学学报（自然科学版） ›› 2025, Vol. 49 ›› Issue (4) : 71-78. DOI: 10.12302/j.issn.1000-2006.202409034

专题报道Ⅱ：发展林业新质生产力系列专题一：林木体细胞胚胎发生专题（执行主编尹佟明陈金慧施季森）

外源脱落酸与低温预处理结合对红松体胚成熟的影响

史玉洁 ¹ ,
任悦 ¹ ,
汤铄然 ¹ ,
杨玲 ¹^,²^,^*

作者信息 +

Effect of exogenous abscisic acid combined with low temperature pretreatment on Pinus koraiensis somatic embryo maturation

SHI Yujie ¹ ,
REN Yue ¹ ,
TANG Shuoran ¹ ,
YANG Ling ¹^,²^,^*

Author information +

文章历史 +

摘要

【目的】阐明外源脱落酸(ABA)结合低温预处理对红松(Pinus koraiensis)体胚成熟的影响,揭示其对红松胚性细胞分化及代谢物积累的作用机制,筛选最佳培养条件。【方法】以红松胚性愈伤组织为材料,采用4种不同时长(0、2、4、6 d)的低温(4 ℃)结合体胚成熟培养基中6种质量浓度梯度(0、5、10、20、50、100 mg/L)的ABA处理,测定愈伤组织在代谢过程中营养物质的活性及相关生理生化指标,系统分析不同处理组合对体胚成熟的影响。【结果】①20 mg/L ABA结合4 ℃低温处理2 d时,红松体胚产量是对照的9.90倍;②该处理贮藏物质质量分数最高,过氧化物酶(POD)和过氧化氢酶(CAT)活性也达到最高,分别为59.45、98.96 U/g,而H₂O₂摩尔质量浓度在这一处理下最低,为0.53 μmol/mg,表明抗氧化系统有效缓解了氧化损伤;③优化培养条件获得的体胚经萌发培养后,再生植株移栽成活率达到45%。【结论】20 mg/L外源ABA结合4 ℃低温预处理2 d是红松体胚发生与成熟的最适条件。本研究可为红松优质品种高效繁殖提供技术支持,进一步推动红松遗传资源的有效保护、优良品种的选育与快速繁育体系的构建。

Abstract

【Objective】This study aims to elucidate the effects of exogenousabscisic acid(ABA) combined with low-temperature pretreatment on the maturation of Pinus koraiensis somatic embryos, unravel the mechanisms regulating embryogenic cell differentiation and metabolite accumulation, and identify the optimal culture conditions.【Method】Using P. koraiensis embryogenic callus as the material, four cold durations (0, 2, 4 and 6 days) at 4 ℃ were combined with six ABA concentrations (0, 5, 10, 20, 50, 100 mg/L) medium. The activity of nutrients and related physiological and biochemical indicators the callus were measured, and the effects of different treatment combinations on somatic embryo maturation were systematically evaluated.【Result】(1) 20 mg/L ABA with two days cold pretreatment increased somatic embryo yield 9.90 times versus control. (2) In this treatment group, the contents of storage substances reached the highest levels, and activities of peroxidase (POD) and catalase (CAT) were also at their peak, reaching 59.45 and 98.96 U/g, respectively. Meanwhile, H₂O₂ content was the lowest at 0.53 μmol/mg, indicating that the antioxidant system effectively mitigated oxidative damage. (3) Somatic embryos obtained under optimized culture conditions exhibited a regeneration rate of 45% after germination and successful transplantation.【Conclusion】The combination of 20 mg/L exogenous ABA with low-temperature pretreatment at 4 ℃ for two days was identified as the optimal condition for somatic embryo induction and maturation in P. koraiensis. This study provides technical support for the efficient propagation of elite P. koraiensis varieties, contributing to the effective conservation of genetic resources, the selection of superior cultivars, and the development of a rapid propagation system.

导出引用

史玉洁, 任悦, 汤铄然, 等. 外源脱落酸与低温预处理结合对红松体胚成熟的影响[J]. 南京林业大学学报（自然科学版）. 2025, 49(4): 71-78 https://doi.org/10.12302/j.issn.1000-2006.202409034

SHI Yujie, REN Yue, TANG Shuoran, et al. Effect of exogenous abscisic acid combined with low temperature pretreatment on Pinus koraiensis somatic embryo maturation[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2025, 49(4): 71-78 https://doi.org/10.12302/j.issn.1000-2006.202409034

中图分类号： S722.3

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