缺硼胁迫对油茶生理及硼转运基因表达的影响

张梦雪; 刘君滢; 范杰; 丁文娜; 罗杰; 梅莉

doi:10.12302/j.issn.1000-2006.202409045

张梦雪, 刘君滢, 范杰, 丁文娜, 罗杰, 梅莉

南京林业大学学报（自然科学版） ›› 2026, Vol. 50 ›› Issue (2) : 119-128.

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南京林业大学学报（自然科学版） ›› 2026, Vol. 50 ›› Issue (2) : 119-128. DOI: 10.12302/j.issn.1000-2006.202409045

研究论文

缺硼胁迫对油茶生理及硼转运基因表达的影响

作者信息 +

The effects of boron deficiency on physiology and boron transport-related genes expression of Camellia oleifera

Author information +

文章历史 +

摘要

【目的】筛选耐低硼油茶(Camellia oleifera)品种,并解析油茶耐低硼的生理和硼转运分子机制。【方法】以7个品种油茶苗为材料,比较不同品种对缺硼胁迫的形态和生理响应,采用隶属函数法评价筛选耐低硼品种;研究4个硼转运基因在耐缺硼和不耐缺硼品种中表达量的差异,初步解析硼高效和硼低效油茶品种的耐性差异机理。【结果】①在缺硼胁迫下,7个品种表现为叶片黄化卷曲,根系褐化脱落及生长受阻等症状。②缺硼胁迫降低7个油茶品种的净光合速率等光合参数,部分品种硼含量显著下降;缺硼胁迫使油茶叶和根中可溶性糖等渗透调节物含量降低,同时也降低过氧化氢、超氧阴离子的含量与超氧化物歧化酶的活性。③隶属函数分析结果表明,‘长林40’(‘Changlin 40’)和‘长林4’(‘Changlin 4’)较耐缺硼,‘华金’(‘Huajin’)最不耐缺硼。④缺硼处理下,4个硼转运基因(CoBOR1、CoBOR1-like、CoBOR2、CoNIP5;1)在‘长林4’和‘华金’中均显著上调表达,耐缺硼品种‘长林4’根系和叶中4个硼转运基因的表达量均显著高于不耐低硼品种‘华金’。【结论】不同油茶品种对缺硼胁迫的耐性差异显著,硼高效品种通过上调表达硼转运基因,提高光合效率、抗氧化及渗透调节能力等提升其耐缺硼能力。

Abstract

【Objective】This study aims to screen boron (B) deficiency tolerant Camellia oleifera cultivars, analyze the physiology of B deficiency tolerance and the molecular mechanism of B transport in C. oleifera.【Method】The physiological responses of seven C. oleifera cultivars seedlings under B deficiency stress were investigated. The affiliation function value method was used to evaluate the resistance of different cultivars to B deficiency. And the tolerance differences between B-efficient and B-inefficient C. oleifera cultivars from the perspective of four B transporter gene expression were analyzed.【Result】(1) Under the B deficiency stress environment, the symptoms of seven cultivars were yellowing and curling of leaves, browning and shedding of roots, and stunting of growth. (2) B deficiency stress reduced the net photosynthetic rate and other photosynthetic parameters, and the B content in some C. oleifera cultivars decreased significantly. The B deficiency reduced the content and activity of osmotic regulators such as soluble sugars, hydrogen peroxide and superoxide anions in leaves and roots, as well as superoxide dismutase. (3) The results of membership function analysis showed that ‘Changlin 40’ and ‘Changlin 4’ were more tolerant to B deficiency, while ‘Huajin’ was the least tolerant to B deficiency. (4) The expressions of four B transporter genes (CoBOR1, CoBOR1-like, CoBOR2, andCoNIP5;1) in ‘Changlin 4’ and ‘Huajin’ were significantly up-regulated, and the expression levels of the four genes in the roots and leaves of the B-deficiency-tolerant cultivar ‘Changlin 4’ were significantly higher than those in the B-deficiency-tolerant cultivar ‘Huajin’.【Conclusion】The tolerance of different C. oleifera cultivars to B deficiency stress varies greatly. The B-efficient cultivars could enhance their ability of boron deficiency tolerance by up-regulating the expression of B transport genes, improving photosynthetic efficiency, antioxidant capacity and osmotic regulation capacity.

导出引用

张梦雪, 刘君滢, 范杰, 等. 缺硼胁迫对油茶生理及硼转运基因表达的影响[J]. 南京林业大学学报（自然科学版）. 2026, 50(2): 119-128 https://doi.org/10.12302/j.issn.1000-2006.202409045

ZHANG Mengxue, LIU Junying, FAN Jie, et al. The effects of boron deficiency on physiology and boron transport-related genes expression of Camellia oleifera[J]. Journal of Nanjing Forestry University (Natural Sciences Edition）. 2026, 50(2): 119-128 https://doi.org/10.12302/j.issn.1000-2006.202409045

中图分类号： S722

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