转录因子等在植物体胚发生中的作用机制

张稼霁; 郝兆东; 鲁路; 郑仁华; 施季森; 陈金慧

doi:10.12302/j.issn.1000-2006.202412025

转录因子等在植物体胚发生中的作用机制

张稼霁, 郝兆东, 鲁路, 郑仁华, 施季森, 陈金慧

南京林业大学学报（自然科学版） ›› 2025, Vol. 49 ›› Issue (4) : 46-56.

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南京林业大学学报（自然科学版） ›› 2025, Vol. 49 ›› Issue (4) : 46-56. DOI: 10.12302/j.issn.1000-2006.202412025

专题报道Ⅱ：发展林业新质生产力系列专题一：林木体细胞胚胎发生专题（执行主编尹佟明陈金慧施季森）

转录因子等在植物体胚发生中的作用机制

张稼霁 ¹ ,
郝兆东 ¹ ,
鲁路 ¹ ,
郑仁华 ² ,
施季森 ¹ ,
陈金慧 ¹^,^*

作者信息 +

Mechanisms of transcription factors and others in somatic embryogenesis of plants

ZHANG Jiaji ¹ ,
HAO Zhaodong ¹ ,
LU Lu ¹ ,
ZHENG Renhua ² ,
SHI Jisen ¹ ,
CHEN Jinhui ¹^,^*

Author information +

文章历史 +

摘要

体胚发生是在植物离体培养条件下实现无性繁殖再生的关键途径之一,该过程通过体细胞重编程形成类似合子胚的结构,最终发育成完整的植株。体胚发生技术作为一种高效的植物再生技术,在植物遗传转化和基因编辑中具有应用潜力,在植物的遗传改良研究中具有重要价值。体胚发生过程涉及复杂的分子调控网络,笔者综述近年来关于植物体胚发生分子调控机制的研究进展,重点探讨关键转录因子,如WOX(WUSCHEL-RELATED HOMEOBOX)、AP2/ERF(APETALA2/ETHYLENE-RESPONSIVE FACTOR)和MADS-box基因家族等,以及相关的信号转导途径。这些转录因子在体胚发生过程中发挥着至关重要的作用。同时深入探讨体胚发生技术在植物育种等生物技术领域的广阔应用前景,随着研究的不断深入,这项技术将在植物生物技术和农林业发展中占据核心地位。

Abstract

Somatic embryogenesis is one of the key ways to achieve asexual reproduction of plants under in vitro culture conditions. The process undergoes somatic cell, treated by the exogenous hormone, reprogramming to form a zygote-like structure that eventually develops into a complete plant. As an efficient plant regeneration technology, somatic embryogenesis has the potential to be applied in plant Agrobacterium-mediated genetic transformation and CRISPR-based genome editing technologies, and is of great value in genetic improvement of plants. Recent advances have revealed that somatic embryogenesis is governed by a hierarchical molecular network involving multiple regulatory tiers. In this review, we review the recent research progress on the molecular regulatory mechanisms of somatic embryogenesis in plants, focusing on the key transcription factors, such as WOX (WUSCHEL-RELATED HOMEOBOX) gene family, AP2/ERF (APETALA2/ETHYLENE-RESPONSIVE FACTOR) and MADS-box gene family, as well as the related signaling pathways in which they are involved in. The members of WOX gene family, particularly WUS and WOX2/5/8/9, play a central role in maintaining stem cell pluripotency and establishing polarity during the development of embryos. The LAFL network (LEC1-ABI3-FUS3-LEC2), integrates hormonal signals, which regulate auxin biosynthesis (YUCs and TAA1) and the balance of endogenous ABA/GA, and epigenetic signals, which modified epigenetically, to regulate somatic embryogenesis. AP2/ERF superfamily members, notably BABY BOOM and PLETHORA 5(PLT5), are essential for somatic cells to acquire totipotency. Members of the MADS-box gene family, including AGL15 and AGL18, regulate somatic embryogenesis mainly by modulating GA-related pathways. These transcription factors play crucial roles in somatic embryogenesis. This paper delves into the broad application prospects of somatic embryogenesis in plant breeding and other biotechnology field. With the deepening of research, this technology will occupy a central position in plant biotechnology and agricultural development.

导出引用

张稼霁, 郝兆东, 鲁路, 等. 转录因子等在植物体胚发生中的作用机制[J]. 南京林业大学学报（自然科学版）. 2025, 49(4): 46-56 https://doi.org/10.12302/j.issn.1000-2006.202412025

ZHANG Jiaji, HAO Zhaodong, LU Lu, et al. Mechanisms of transcription factors and others in somatic embryogenesis of plants[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2025, 49(4): 46-56 https://doi.org/10.12302/j.issn.1000-2006.202412025

中图分类号： S722

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