长期氮添加对兴安落叶松和水曲柳人工林根系磷获取策略的影响

尹天龙; 刘志; 任浩; 马耀远; 谷加存

doi:10.12302/j.issn.1000-2006.202501024

尹天龙, 刘志, 任浩, 马耀远, 谷加存

南京林业大学学报（自然科学版） ›› 2026, Vol. 50 ›› Issue (1) : 150-159.

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南京林业大学学报（自然科学版） ›› 2026, Vol. 50 ›› Issue (1) : 150-159. DOI: 10.12302/j.issn.1000-2006.202501024

研究论文

长期氮添加对兴安落叶松和水曲柳人工林根系磷获取策略的影响

作者信息 +

Effects of long-term nitrogen addition on root phosphorus acquisition strategy in Larix gmelinii and Fraxinus mandshurica plantations

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文章历史 +

摘要

【目的】揭示长期氮(N)添加对我国东北地区人工林根系磷(P)获取策略的影响,为深入理解根系经济空间理论框架提供参考。【方法】以黑龙江省帽儿山地区兴安落叶松(Larix gmelinii)和水曲柳(Fraxinus mandshurica)人工林为对象,研究长期N添加对2树种土壤表层(0~10 cm)和亚表层(11~20 cm)根系磷酸酶活性(RPA)的影响,并分析RPA与根系形态、菌根侵染程度和根际土壤属性之间的联系。【结果】长期N添加下兴安落叶松和水曲柳根际土壤有效磷和磷酸酶活性有增加趋势。两树种RPA由1级根至3级根逐渐下降,但在土层间差异不显著。同一根序下,水曲柳RPA显著高于兴安落叶松。长期N添加导致兴安落叶松和水曲柳吸收根(前3级根)总RPA下降,尤其是在水曲柳1级根中差异显著。吸收根形态在长期N添加后也发生不同程度的变化,呈现出直径更细、组织密度更低的趋势。主成分分析显示两树种根系功能性状具有多维经济谱特征,两树种RPA与菌根侵染率、根际土壤有效P和磷酸酶活性呈负相关,而与比根长、比根表面积呈正相关,表明RPA在真菌协作维度(fungal collaboration gradient)的“自己动手”(do-it-yourself)策略上发挥作用。【结论】长期N添加后兴安落叶松和水曲柳人工林的P获取策略发生改变,根系变得细长,RPA和菌根侵染率降低,且这种影响在水曲柳人工林中更加明显。

Abstract

【Objective】 The objective of this study was to reveal the effects of long-term nitrogen (N) addition on root phosphorus (P) acquisition strategies of plantations in northeast China, providing a fundamental understanding in the framework of root economics space. 【Method】Following a 20-year N-addition manipulation in Larix gmelinii and Fraxinus mandshurica plantations in Maoershan, Heilongjiang Province, China, we investigated the effect of long-term N addition on root phosphatase activity (RPA) in the surface (0-10 cm) and subsurface (11-20 cm) soils, and explored the relationship between RPA and root morphology, mycorrhizal colonization rate, and rhizosphere soil properties. 【Result】Long-term nitrogen (N) addition promoted an increasing trend in rhizosphere soil available phosphorus and phosphatase activity for both L. gmelinii and F. mandshurica. Root phosphatase activity (RPA) progressively declined from first-to third-order roots in both species, though no significant differences were observed between soil layers. Within the same root order, F. mandshurica consistently exhibited higher RPA than L. gmelinii. Prolonged N addition significantly reduced the total RPA of absorptive roots (first three orders) in both species, with the most pronounced reduction occurring in the first-order roots of F. mandshurica. Additionally, absorptive roots displayed morphological adjustments under N enrichment, including finer root diameters and lower tissue density. Principal component analysis revealed multidimensional coordination of root functional traits, highlighting negative correlations between RPA and mycorrhizal colonization rate, rhizosphere available phosphorus, and bulk soil phosphatase activity, but positive correlations with specific root length and surface area. These patterns collectively indicate that RPA aligns with a “do-it-yourself” phosphorus acquisition strategy along the fungal collaboration gradient, favoring enzymatic investment over symbiotic dependency under N-enriched conditions. 【Conclusion】Collectively, after long-term N addition, the P acquisition strategies of the two trees changed, roots became slender, RPA and the mycorrhizal colonization rate decreased, which were more obvious in F. mandshurica plantations.

导出引用

尹天龙, 刘志, 任浩, 等. 长期氮添加对兴安落叶松和水曲柳人工林根系磷获取策略的影响[J]. 南京林业大学学报（自然科学版）. 2026, 50(1): 150-159 https://doi.org/10.12302/j.issn.1000-2006.202501024

YIN Tianlong, LIU Zhi, REN Hao, et al. Effects of long-term nitrogen addition on root phosphorus acquisition strategy in Larix gmelinii and Fraxinus mandshurica plantations[J]. Journal of Nanjing Forestry University (Natural Sciences Edition）. 2026, 50(1): 150-159 https://doi.org/10.12302/j.issn.1000-2006.202501024

中图分类号： S718

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