
平安竹缩短节间形成相关转录因子PjGRAS56功能研究
刘世婴, 蒋嘉雯, 高志鹏, 魏强
南京林业大学学报(自然科学版) ›› 2025, Vol. 49 ›› Issue (4) : 12-22.
平安竹缩短节间形成相关转录因子PjGRAS56功能研究
Functional characterization of PjGRAS56, a transcription factor involved in shortened internode formation in Pseudosasa japonica var. tsutsumiana
【目的】竹子的节间长度是竹材产业利用的重要性状。平安竹(Pseudosasa japonica var. tsutsumiana)为矢竹(P. vjaponica)的稳定矮秆变异体,节间缩短成花瓶状,是研究竹类节间生长的理想材料。前期研究分析发现GRAS转录因子PjGRAS56为平安竹缩短节间形成的关键因子。本研究旨在解析PjGRAS56在竹子节间生长发育中的潜在作用。【方法】利用生物信息学分析PjGRAS56序列特征,激光共聚焦观察其亚细胞定位,酵母转录系统分析转录因子激活活性,水稻(Oryza sativa)与拟南芥(Arabidopsis thaliana)转基因技术分析过表达PjGRAS56植株表型,转录组测序与qRT-PCR分析基因表达变化,徒手切片观察节间细胞长度。【结果】PjGRAS56定位于细胞核与细胞质中,无转录激活活性,且在平安竹节间分裂区中显著下调表达;水稻PjGRAS56过表达植株矮化、节间缩短、分蘖数减少、穗长和种子芒长变短、籽粒数减少,同时叶夹角变小;而在拟南芥中,过表达植株叶片缩小、花茎变短;水稻PjGRAS56过表达植株的节间细胞长度与对照植株无显著差异,但细胞数目显著下降。转录组测序与差异表达基因分析显示,细胞分裂相关基因在过表达植株中显著下调表达。【结论】PjGRAS56是竹子节间生长潜在负调控因子。
【Objective】Internode length is a crucial trait for bamboo industrial utilization. Pseudosasa japonica var. tsutsumiana is a stable dwarf mutant of P. japonica, characterized by shortened, flask-shaped internodes, making it an ideal material for studying internode growth in bamboos. Preliminary studies identified the GRAS transcription factor PjGRAS56 as a key candidate gene responsible for the shortened internode formation in P. japonica var. tsutsumianais. This study aims to elucidate the potential role of PjGRAS56 in bamboo internode development.【Method】We employed bioinformatics to analyze the sequence features of PjGRAS56, confocal laser scanning microscopy to observe its subcellular localization, a yeast transcription system to assess its transcriptional activation activity, transgenic techniques in rice and Arabidopsis to analyze phenotypes of PjGRAS56-overexpressing plants, transcriptome sequencing and qRT-PCR to examine gene expression changes, and hand-sectioning to measure internode cell length.【Result】PjGRAS56 localized to the nucleus and cytoplasm, and lacked transcriptional activation activity. It was significantly down-regulated in the division zone of P. japonica var. tsutsumianais internodes. Overexpression of PjGRAS56 in rice resulted in dwarfism, shortened internodes, reduced tillering, shorter panicles and awns, fewer grains, and reduced leaf angle; overexpression of it in Arabidopsis led to smaller leaves and shorter flower stalks. Although the cell length in internodes of PjGRAS56-overexpressing plants was similar to controls, the cell number was significantly reduced. Transcriptome sequencing and differential gene expression analysis revealed significant down-regulation of cell division-related genes in PjGRAS56-overexpressing plants.【Conclusion】PjGRAS56 is a putative negative regulator of internode growth in bamboo.
平安竹 / 节间长度 / 居间分生组织 / 分裂区 / PjGRAS56
Pseudosasa japonica var. tsutsumiana / internode length / intercalary meristem / division zone / PjGRAS56
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