杂交鹅掌楸良种‘南林-金森 E1 号’器官离体再生体系的建立

殷健超; 杨顶杰; 郝兆东; 郑仁华; 施季森; 陈金慧

doi:10.12302/j.issn.1000-2006.202510001

殷健超, 杨顶杰, 郝兆东, 郑仁华, 施季森, 陈金慧

南京林业大学学报（自然科学版） ›› 2026, Vol. 50 ›› Issue (4) : 75-83.

PDF(28935 KB)

国家林草科技领军期刊
中国精品科技期刊
中国高校百佳科技期刊
江苏省新闻出版政府奖期刊奖
RCCSE林学权威期刊（A+）
CSCD核心期刊
Scopus数据库收录期刊
中文核心期刊
SCD核心期刊

作者加群：102861116

微信公众号：南京林业大学学报

高级检索

PDF(28935 KB)

南京林业大学学报（自然科学版） ›› 2026, Vol. 50 ›› Issue (4) : 75-83. DOI: 10.12302/j.issn.1000-2006.202510001

第二十八届中国科协年会———全球气候变化下的林草智能设计育种专题（执行主编　曹福亮　范国强　尹佟明　张怀清）
专题报道Ⅰ

杂交鹅掌楸良种‘南林-金森 E1 号’器官离体再生体系的建立

殷健超 ¹ ,
杨顶杰 ¹ ,
郝兆东 ¹ ,
郑仁华 ² ,
施季森 ¹ ,
陈金慧 ¹^,^*

作者信息 +

Establishment of in vitro regeneration system for organs of Liriodendron hybrids elite cultivar L. × sino-americanum ‘Nanlin-Jinsen E1’

Author information +

文章历史 +

摘要

【目的】利用拥有自主知识产权的杂交鹅掌楸(Liriodendron hybrid)优良品种‘南林-金森 E1 号’(L. × sino-americanum ‘Nanlin-Jinsen E1’)的茎段和茎尖作为试验材料,通过组织培养技术,构建杂交鹅掌楸优良品种的器官离体再生体系。【方法】利用茎段、茎尖外植体开展杂交鹅掌楸良种的不定芽诱导、伸长培养、生根培养,最终获得完整再生植株,建立起稳定高效的器官离体再生体系。【结果】不定芽诱导的最佳基本培养基为MS培养基;茎段外植体不定芽诱导的最佳培养基为MS + 0.50 mg/L 6-BA + 0.100 mg/L NAA + 0.005 mg/L TDZ;茎尖外植体不定芽诱导的最佳培养基为MS + 0.50 mg/L 6-BA +0.100 mg/L NAA + 0.020 mg/L TDZ;伸长培养的最佳植物生长调节剂与培养基组合为MS + 0.01 mg/L GA₃;生根培养的最佳组合为1/2 MS + 0.300 mg/L NAA;生根培养的最佳蔗糖质量浓度为20.0 g/L。【结论】以茎段、茎尖为外植体,筛选杂交鹅掌楸良种在不定芽诱导、伸长培养、生根培养中的最佳组合,建立杂交鹅掌楸良种器官离体再生体系,为杂交鹅掌楸良种的优快繁殖提供一条新的有效途径。

Abstract

【Objective】The objective of this study is to establish an in vitro organ regeneration system for the Liriodendron hybrid elite cultivar L. × sino-americanum ‘Nanlin-Jinsen E1’. This will be achieved using stem segments and shoot apical meristems from the Liriodendron hybrid elite cultivar, which possesses independent intellectual property rights, as experimental materials through tissue culture.【Method】Using stem segments and stem tips as explants, we conducted bud induction, elongation culture, and rooting culture of L. × sino-americanum ‘Nanlin-Jinsen E1’, ultimately obtaining fully regenerated plants and establishing a stable and efficient organ regeneration system in vitro.【Result】The optimal basic medium for inducing adventitious buds is MS medium; the optimal culture medium for inducing adventitious buds from stem explants is MS + 0.50 mg/L 6-BA + 0.100 mg/L NAA + 0.005 mg/L TDZ; the optimal culture medium for inducing adventitious buds from stem tip explants is MS + 0.50 mg/L 6-BA + 0.100 mg/L NAA + 0.020 mg/L TDZ; the optimal medium for plant growth regulators for elongation culture is MS + 0.01 mg/L GA₃; the optimal medium for rooting culture is 1/2 MS + 0.300 mg/L NAA; the optimal sucrose concentration for rooting culture is 20.0 g/L.【Conclusion】Using stem segments and tips as explants, the optimal experimental conditions for inducing adventitious buds, elongation culture, and rooting culture of L. × sino-americanum ‘Nanlin-Jinsen E1’ were obtained. A system for in vitro organ regeneration of L. × sino-americanum ‘Nanlin-Jinsen E1’ was established, providing a new and effective way for the optimal and rapid reproduction of Liriodendron hybrids excellent variety.

导出引用

殷健超, 杨顶杰, 郝兆东, 等. 杂交鹅掌楸良种‘南林-金森 E1 号’器官离体再生体系的建立[J]. 南京林业大学学报（自然科学版）. 2026, 50(4): 75-83 https://doi.org/10.12302/j.issn.1000-2006.202510001

Yin Jianchao, Yang Dingjie, Hao Zhaodong, et al. Establishment of in vitro regeneration system for organs of Liriodendron hybrids elite cultivar L. × sino-americanum ‘Nanlin-Jinsen E1’[J]. Journal of Nanjing Forestry University (Natural Sciences Edition）. 2026, 50(4): 75-83 https://doi.org/10.12302/j.issn.1000-2006.202510001

中图分类号： S722

参考文献

列表( 原文顺序 | 文献年度倒序 | 文中引用次数倒序 ) 可视化分析

[1]	魏一鸣. 浅析鹅掌楸的园林价值[J]. 河北农机, 2021(1):121-122. Wei Y M. Analysis on the garden value of Liriodendron chinense[J]. Hebei Agricultural Machinery, 2021(1):121-122. DOI:10.15989/j.cnki.hbnjzzs.2021.01.067. 本文引用 [1]

[2]	郝日明, 贺善安, 汤诗杰, 等. 鹅掌楸在中国的自然分布及其特点[J]. 植物资源与环境, 1995, 4(1):1-6. Hao R M, He S A, Tang S J, et al. Geographical distribution of Liriodederon chinense in China and its significance[J]. Journal of Plant Resources and Environment, 1995, 4(1):1-6. 本文引用 [1]

[3]	臧春鑫. 《中国生物多样性红色名录》解读[J]. 中华环境, 2016(9):33-36. Zang C X. Interpretation of the red list of biodiversity in China[J]. China Environment, 2016(9):33-36. 本文引用 [1]

[4]	田敏, 李纪元, 范正琪. 杂交鹅掌楸离体培养中器官发生的研究[J]. 林业科学研究, 2005, 18(5):546-550. Tian M, Li J Y, Fan Z Q. Study on organogenesis of Liriodendron chinense × L.tulipifera[J]. Forest Research, 2005, 18(5):546-550. DOI:10.13275/j.cnki.lykxyj.2005.05.008. 本文引用 [1]

[5]	Li B, Lin F R, Huang P, et al. Development of nuclear SSR and chloroplast genome markers in diverse Liriodendron chinense germplasm based on low-coverage whole genome sequencing[J]. Biological Research, 2020, 53(1):21. DOI:10.1186/s40659-020-00289-0. 本文引用 [1]

[6]	陈金慧, 施季森, 诸葛强, 等. 杂交鹅掌楸体细胞胚胎发生研究[J]. 林业科学, 2003, 39(4):49-53,177. Chen J H, Shi J S, Zhuge Q, et al. Studies on the somatic embryogenesis of Liriodendron hybrids(L.chinense × L.Tulipifera)[J]. Scientia Silvae Sinicae, 2003, 39(4):49-53,177. 本文引用 [2]

[7]	李婷婷, 陈金慧, 龙伟, 等. 杂交鹅掌楸体胚发生悬浮系建立及同步化发育的调控研究[C]// 第六届全国林木遗传育种大会论文集. 杭州,2008:88-89. 本文引用 [1]

[8]

陈婷婷, 王鹏凯, 张稼霁, 等. 脱落酸和玉米素结合使用对杂交鹅掌楸体胚分化及发育的影响[J]. 林业科学, 2019, 55(3):64-71.

Chen

T T

, Wang

P K

, Zhang

J J

, et al. Effects of combined ABA and ZT treatment on somatic embryogenesis and development of Liriodendron sino-americanum[J]. Scientia Silvae Sinicae, 2019, 55(3):64-71. DOI:10.11707/j.1001-7488.20190307.

本文引用 [1]

[9]

鲁路, 陆叶, 盛宇, 等. 不同活性炭对杂交鹅掌楸体胚发生的影响[J]. 南京林业大学学报(自然科学版), 2016, 40(2):59-64.

, Lu

, Sheng

, et al. Effects of different activated carbon on somatic embryogenesis of Liriodendron hybrids[J]. Journal of Nanjing Forestry University (Natural Sciences Edition), 2016, 40(2):59-64. DOI:10.3969/j.issn.1000-2006.2016.02.010.

本文引用 [1]

[10]	CHEN X Y, LIU Y, LU L, et al. Establishment of a glucocorticoid inducible system for regulating somatic embryogenesis in Liriodendron hybrids[J]. Forestry Research, 2024, 4:e006. DOI:10.48130/forres-0024-0003. 本文引用 [1]

[11]	Zhang J J, Hao Z D, Ruan X X, et al. Role of BABY BOOM transcription factor in promoting somatic embryogenesis and genetic transformation in a woody magnoliid Liriodendron[J]. Plant,Cell & Environment, 2025, 48(7):4859-4872. DOI:10.1111/pce.15483. 本文引用 [1]

[12]	赵翔宇. 植物组织培养在林木遗传育种中的应用[J]. 河南农业, 2022(11):51-52. Zhao X Y. Application of plant tissue culture in forest genetic breeding[J]. Agriculture of Henan, 2022(11):51-52. DOI:10.15904/j.cnki.hnny.2022.11.011. 本文引用 [1]

[13]	Cardoso J C, Sheng Gerald L T, Teixeira da Silva J A. Micropropagation in the twenty-first century[M]//Plant Cell Culture Protocols. New York: Springer,2018:17-46. DOI:10.1007/978-1-4939-8594-4_2. 本文引用 [1]

[14]	Steward F C, Mapes M O, Kent A E, et al. Growth and development of cultured plant cells:biochemical and morphogenetic studies with cells yield new evidence on their metabolism and totipotency[J]. Science, 1964, 143(3601):20-27. DOI:10.1126/science.143.3601.20. 本文引用 [1]

[15]

高燕, 莫建彬, 付艳茹, 等. 铁线莲‘朱卡’组织培养技术及再生体系的建立[J]. 南京林业大学学报(自然科学版), 2021, 45(3):109-116.

Gao

, Mo

J B

, Fu

Y R

, et al. Tissue culture and plant regeneration of Clematis ‘Julka’[J]. Journal of Nanjing Forestry University (Natural Sciences Edition), 2021, 45(3):109-116. DOI:10.12302/j.issn.1000-2006.201912012.

本文引用 [1]

[16]

孙永莲, 戴晓港, 李小平, 等. 簸箕柳组培再生体系的建立[J]. 南京林业大学学报(自然科学版), 2019, 43(2):31-37.

Sun

Y L

, Dai

X G

, Li

X P

, et al. Plant regeneration of Salix suchowensis through tissue culture[J]. Journal of Nanjing Forestry University (Natural Sciences Edition), 2019, 43(2):31-37. DOI:10.3969/j.issn.1000-2006.201801034.

本文引用 [1]

[17]	Šmeringai J, Schrumpfová P P, Pernisová M. Cytokinins-regulators of de novo shoot organogenesis[J]. Frontiers in Plant Science, 2023, 14:1239133. DOI:10.3389/fpls.2023.1239133. 本文引用 [1]

[18]	刘霞, 孙冲. 木本植物试管苗玻璃化成因与控制研究进展[J]. 西南林业大学学报, 2013, 33(5):93-97. Liu X, Sun C. Causes and control of vitrification on the plantlets of woody plants[J]. Journal of Southwest Forestry College, 2013, 33(5):93-97. DOI:10.3969/j.issn.2095-1914.2013.05.017. 本文引用 [1]

[19]	Zeng Q Q, Han Z Q, Kang X Y. Adventitious shoot regeneration from leaf,petiole and root explants in triploid (Populus alba × P.glandulosa) × P.tomentosa[J]. Plant Cell,Tissue and Organ Culture (PCTOC), 2019, 138(1):121-130. DOI:10.1007/s11240-019-01608-4. 本文引用 [1]

[20]

黄娟, 吴鹏飞, 李惠通, 等. 杉木不同器官愈伤组织和再生芽的诱导效率[J]. 森林与环境学报, 2017, 37(2):129-135.

Huang

, Wu

P F

, Li

H T

, et al. Efficiency of callus and regeneration bud induction from different explants of Cunninghamia lanceolata[J]. Journal of Forest and Environment, 2017, 37(2):129-135. DOI:10.13324/j.cnki.jfcf.2017.02.001.

本文引用 [1]

[21]	Du M M, Bou Daher F, Liu Y Y, et al. Biphasic control of cell expansion by auxin coordinates etiolated seedling development[J]. Science Advances, 2022, 8(2):eabj1570. DOI:10.1126/sciadv.abj1570. 本文引用 [1]

[22]

Mirheidari

, Hatami

, Ghorbanpour

. Effect of different concentrations of IAA,GA3 and chitosan nano-fiber on physio-morphological characteristics and metabolite contents in Roselle (Hibiscus sabdariffa L.)[J]. South African Journal of Botany, 2022, 145:323-333. DOI:10.1016/j.sajb.2021.07.021.

本文引用 [1]

[23]	Habibi S, Ebadi A, Ladanmoghadam A R, et al. Effect of plant growth regulators on fruit splinting in Thompson navel orange[J]. Acta Scientiarum Polonorum Hortorum Cultus, 2021, 20(2):83-92. DOI:10.24326/asphc.2021.2.9. 本文引用 [1]

[24]	Chen L, Hao L G, Condon A G, et al. Exogenous GA₃ application can compensate the morphogenetic effects of the GA-responsive dwarfing gene Rht12 in bread wheat[J]. PLoS One, 2014, 9(1):e86431. DOI:10.1371/journal.pone.0086431. 本文引用 [1]

[25]	Siddiqua A, Thippesha D, Shivakumar B, et al. Effect of growth regulators on rooting and shooting of stem cuttings in dragon fruit [Hylocereus undatus (Haworth) Britton & rose][J]. Journal of Pharmacognosy and Phytochemistry, 2018, 7(5):1595-1598. 本文引用 [1]

[26]	Kumar R, Singh A K, Tomar K S, et al. Effect of plant growth regulators on rooting of Bougainvillea var.‘Manila Red’ through hardwood cuttings[J]. Journal of Ornamental Horticulture, 2021, 24(1):58-62. DOI:10.5958/2249-880x.2021.00009.8. 本文引用 [1]

[27]	刘艳艳, 司灿, 何春梅, 等. 龙珠果茎段离体培养和组培苗耐盐性分析[J]. 热带亚热带植物学报, 2021, 29(4):360-366. Liu Y Y, Si C, He C M, et al. Stem culture in vitro of Passiflora foetida and salt tolerance of seedlings[J]. Journal of Tropical and Subtropical Botany, 2021, 29(4):360-366. DOI:10.11926/jtsb.4323. 本文引用 [1]

[28]	Cui X H, Murthy H N, Wu C H, et al. Sucrose-induced osmotic stress affects biomass,metabolite,and antioxidant levels in root suspension cultures of Hypericum perforatum L[J]. Plant Cell,Tissue and Organ Culture (PCTOC), 2010, 103(1):7-14. DOI:10.1007/s11240-010-9747-z. 本文引用 [1]

[29]

Baque

M A

, Elgirban

, Lee

E J

, et al. Sucrose regulated enhanced induction of anthraquinone,phenolics,flavonoids biosynthesis and activities of antioxidant enzymes in adventitious root suspension cultures of Morinda citrifolia (L.)[J]. Acta Physiologiae Plantarum, 2012, 34(2):405-415. DOI:10.1007/s11738-011-0837-2.

本文引用 [1]

[30]	Li K P, Han H, Xie Y H, et al. Optimization of factors influencing adventitious rooting in hybrid larch[J]. Phyton, 2021, 90(2):583-593. DOI:10.32604/phyton.2021.013912. 本文引用 [1]