以红白忍冬和郁香忍冬盆栽苗为试验材料,研究不同浓度的NaCl胁迫对两者生理指标的影响,并比较它们的耐盐性差异。结果表明,在盐胁迫条件下,两种植物叶片的电导率、MDA含量均不断上升。胁迫期间,红白忍冬的H2O2含量相对于对照没有明显变化,O</sup><sup>-· 2产生速率在处理前期明显上升,上升出现的时间迟于郁香忍冬; 而郁香忍冬的H2O2含量一直维持较高水平,O</sup><sup>-· 2产生速率在处理前期也有所增加。红白忍冬的SOD、APX和CAT活性总体表现出先上升后下降的趋势,而POD活性则一直上升。郁香忍冬在胁迫期间,其SOD活性有小幅增加; APX和CAT的活性均呈现先上升后下降的趋势; POD活性前期没有明显增加,在处理终期时也仅在100 mmol/L胁迫下才显著增加。表明两种植物在遭受盐胁迫时,均能启动抗氧化酶系统来清除活性氧,降低氧化胁迫的伤害。同时,相对于郁香忍冬,红白忍冬通过快速上调抗氧化酶活性,降低盐胁迫对膜脂过氧化作用,保持膜的完整性,从而更好地抵御氧化损害更耐受盐胁迫。
Abstract
Lonicera japonica var. chinensis and L.fragrantissima potted plants were used to study their physiological response and salt tolerance under NaCl stress. The results showed that the electrical conductivity and MDA content increased with the increasing of salt concentration in both species. There was no obvious rise of H2O2 content in L. japonica var. chinensis, while a higher level of H2O2 content in L.fragrantissima was observed than control. There was a significant generating rate of superoxide radical(O</sup><sup>-· 2)at early stage of salt stress in L. japonica var. chinensis, but the time was later than superoxide radical in L.fragrantissima. For L. japonica var. chinensis,the activities of SOD, APX, and CAT increased and then decreased, while the POD activity showed a constant increase during stress process. For L.fragrantissima, the SOD activity increased slightly during NaCl stress, however, the activities of APX and CAT increased first and then decreased. No obvious POD activity increment was detected, only a significant increment was observed under at the end of 100 mmol/L NaCl stress. These results suggested that the two Lonicera species could employantioxidant enzymes to eliminate ROS under salt stress. Meanwhile, the salt tolerance of L. japonica var. chinensis was better than that of L.fragrantissima by rapidly increasing the activities of antioxidant enzymes to decrease the lipid peroxidation level and reduce ROS damage.
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参考文献
[1] Zhu J K. Salt and drought stress signal transduction in plants[J]. Ann Rev Plant Biol, 2002, 53:247-273.
[2] Jiang M Y, Zhang J H. Effect of abscisic acid on oxygen species, antioxidative defence system and oxidative damage in leaves of maize seedlings[J]. Plant Cell Physiol, 2001, 42(11):1265-1273.
[3] 张玲艳. 活性氧在植物信号转导中的作用[J]. 科技创业月刊, 2005,18(6): 144-145. Zhang L Y. Function of ROS in signal transduction of plant[J]. Ploneering with Science & Technology, 2005,18(6): 144-145.
[4] 廖岩,彭友贵,陈桂珠. 植物耐盐性机理研究进展[J]. 生态学报, 2007, 27(5):2077-2089. Liao Y, Peng Y G, Chen G Z. Research advances in plant salt-tolerance mechanism[J]. Acta Ecologica Sinica, 2007, 27(5):2077-2089.
[5] 胡晓立,李彦慧,陈东亮,等. 3种李属彩叶植物对NaCl胁迫的生理响应[J]. 西北植物学报,2010, 30(2): 370-376. Hu X L, Li Y H, Chen D L, et al. Physiological responses of three colored leaf species of Prunus under NaCl stress[J]. Acta Bot Boreal, 2010, 30(2):370-376.
[6] 陈健妙,郑青松,刘兆普,等. 两种麻疯树苗对盐胁迫的生理生态响应[J]. 生态学报, 2010, 30(4): 933-940. Chen J M, Zheng Q S, Liu Z P, et al. Response characteristics of physiology and ecology to salt stresses in two varieties of Jatropha curcas L. seedlings[J]. Acta Ecologica Sinica, 2010, 30(4):933-940.
[5] 杜佳朋, 张友民, 王豫. 忍冬属植物的研究进展[J]. 北方园艺, 2005(4): 11-13. Du J P, Zhang Y M, Wang Y. Progress in research of Lonicera plants[J]. Northern Horticulture, 2005(4):11-13.
[6] 张金政,梁松洁,石雷. 忍冬属藤本植物资源的栽培及应用[J]. 中国园林, 2004(5): 53-55. Zhang J Z, Liang S J, Shi L. The study on the cultivation and application of climbing honeysuckle resource[J]. Journal of Chinese Landscape Architecture, 2004(5): 53-55.
[7] 梁松洁. 北方地区八种藤本忍冬抗旱性比较研究[D]. 北京:北京林业大学, 2004. Liang S J. Comparative studies on drought resistance of 8 species of climbing honeysuckle(Lonicera)[D]. Beijing: Beijing Forestry University, 2004.
[8] 赵贤慧. 红金银花繁殖技术及抗寒性[D]. 青岛:青岛农业大学, 2007. Zhao X H. The propagation technology and cold tolerance of Lonicera japonica var. chinensis[D]. Qingdao: Qingdao Agricultural University, 2007.
[9] 许思臣, 郭宪清, 刘进社,等. 河南省郁香忍冬(糖果树)野生资源分布及开发利用研究[J]. 河南林业科技, 1999, 19(1): 8-10. Xu S C, Guo X Q, Liu J S, et al. Distribution and exploitation of wild resource of Lonicera frangrantissuma Lindl in Henan province[J]. Journal of Henan Forestry Science and Technology, 1999, 19(1):8-10.
[10] 郑晓军,马宏伟 郭二辉,等. 河南忍冬属植物观赏特性评价及园林应用研究[J]. 河南科学,2009, 27(10): 1236-1238. Zheng X J, Ma H W, Guo E H, et al. Evaluation on the ornamental characteristics of Lonicera plants in Henan and their uses in Garden[J]. Henan Science, 2009, 27(10):1237-1238.
[11] 郭现清. 河南省郁香忍冬种质资源调查与开发利用研究[D]. 南京:南京林业大学, 2006. Guo X Q. Research and utilization of the wild lonicera fragrantissima resources in Henan province[D]. Nanjing: Nanjing Forestry University, 2006.
[12] 赵可夫,李军. 盐浓度对3种单子叶盐生植物渗透调节剂及其在渗透调节中贡献的影响[J]. 植物学报,1999, 41(12): 1287-1292. Zhao K F, Li J. Effects of salinity on the contents of osmotica of monocotyledenous halophytes and their contribution to osmotic adjustment[J]. Acta Botanica Sinica, 1999, 41(12):1287-1292.
[13] 郝建军,康宗利,于洋. 植物生理学实验技术[M]. 北京:化学工业出版社, 2007.
[14] 陈建勋,王晓峰. 植物生理实验指导[M]. 广州:华南理工大学科学出版社, 2006.
[15] 柯德森,王爱国,罗广华. 乙烯促进线粒体呼吸过程中活性氧的作用[J]. 热带亚热带植物学报, 1999, 7(2): 140-145. Ke D S, Wang A G, Luo G H. The effect of active oxygen on mitochondrial respiration induced by exogenous ethylene[J]. Journal of Tropical and Subtropical Botany, 1999, 7(2):140-145.
[16] 李忠光,宋玉泉,龚明. 二甲酚橙法用于测定植物组织中的过氧化氢[J]. 云南师范大学学报:自然科学版, 2007, 27(3): 50-54. Li Z G, Song Y Q, Gong M. Xylenol orange method used for the measurement of hydrogen peroxide in plant tissue[J]. Journal of Yunnan Normal University:Natural Sciences Edition, 2007, 27(3):50-54.
[17] Mittova V, Tal M, Volokita M, et al. Salt stress induces up-regulation of an efficient chloroplast antioxidant system in the salt-tolerant wild tomato species Lycopersicon pennellii but not in the cultivated species[J]. Physiol Plant, 2002, 115(3): 393-400.
[18] 陈健妙,郑青松,刘兆普,等. 麻疯树(Jatropha curcas L.)幼苗生长和光合作用对盐胁迫的响应[J]. 生态学报, 2009, 29(3): 1356-1365. Chen J M, Zheng Q S, Liu Z P, et al. Growing and photosynthetic response of Jatropha curcas L. seedlings to salt stress[J]. Acta Ecologica Sinica, 2009, 29(3):1356-1365.
[19] Jiang M Y, Zhang J H. Water stress-induced abscisic acid accumulation triggers the increased generation of reactive oxygen species and up-regulates the activities of antioxidant enzymes in maize leaves[J]. J Expt Bot, 2002, 53(379): 2401-2410.
[20] 秦小琼,贾士荣. 植物抗氧化逆境的基因工程[J]. 农业生物技术学报, 1997, 5(1): 14-24. Qin X Q, Jia S R. Genetic engineering of plants tolerant to oxidative stress[J]. Journal of Atricutural Biotechnology, 1997, 5(1):14-24.
[21] 陈少裕. 膜脂过氧化对植物细胞的伤害[J]. 植物生理学通讯, 1991, 27(2): 84-90. Chen S Y. Injury of membrane lipid peroxidation to plant cell[J]. Plant Physiology Communications, 1991, 27(2):84-90.
基金
收稿日期:2012-03-23 修回日期:2012-09-10
基金项目:“十一五”国家科技支撑计划(2008BAJ10B04); 上海市科委项目(09dz1205003)
第一作者:程淑娟,硕士生。*通信作者:唐东芹,副教授,博士。E-mail: dqtang@sjtu.edu.cn。
引文格式:程淑娟,唐东芹,刘群录. 盐胁迫对两种忍冬属植物活性氧平衡的影响[J]. 南京林业大学学报:自然科学版,2013,37(1):137-141.
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