抗松材线虫赤松组培繁殖优良家系的筛选

李清清; 叶建仁; 吴小芹

doi:10.3969/j.issn.1000-2006.2013.02.009

抗松材线虫赤松组培繁殖优良家系的筛选

李清清,叶建仁,吴小芹

南京林业大学学报（自然科学版） ›› 2013, Vol. 37 ›› Issue (02) : 50-54.

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南京林业大学学报（自然科学版） ›› 2013, Vol. 37 ›› Issue (02) : 50-54. DOI: 10.3969/j.issn.1000-2006.2013.02.009

研究论文

抗松材线虫赤松组培繁殖优良家系的筛选

李清清,叶建仁^*,吴小芹

作者信息 +

Selection of wilt-resistant Pinus densiflora superior families for tissue culture propagation

LI Qingqing,YE Jianren^*, WU Xiaoqin

Author information +

文章历史 +

摘要

以抗松材线虫赤松(简称抗病赤松)27个家系带子叶顶芽的胚苗为外植体,筛选生长势好、增殖系数高和生根能力强的组培繁殖优良家系。结果表明:1、8、10、13、22和25家系的平均伸长率都达到了100%以上; 随着增殖代数的增加,各家系丛生芽增殖系数表现不一致,但总体趋势是l至2代丛生芽增殖系数随代数增加而增加,2至3代增殖系数随代数增加而下降; 抗病赤松不同无性系不定根诱导率不同,19-B无性系的不定根诱导率最高为95.8%。

Abstract

Using the embryo seedlings with cotyledons of 27 wilt-resistant Pinus densiflora families as explants, superior families which were good growth vigor, high multiplication rate and strong rooting ability were selected. The average elongation percentages of No.1, 8, 10, 13, 22 and 25 families were up to above 100%; With the increase of proliferation generations, the multiplication coefficient of clustered shoots in each family was variable, but the overall trend was that multiplication coefficient of clustered shoots from generation 1 to generation 2 increased with generation increase, and multiplication coefficient from generation 2 to generation 3 decreased; Adventitious root induction rate of wilt-resistant P. densiflora clones was different, 19-B clone of wilt-resistant P. densiflora was the highest,up to 95.8%.

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李清清,叶建仁,吴小芹. 抗松材线虫赤松组培繁殖优良家系的筛选[J]. 南京林业大学学报（自然科学版）. 2013, 37(02): 50-54 https://doi.org/10.3969/j.issn.1000-2006.2013.02.009

LI Qingqing,YE Jianren, WU Xiaoqin. Selection of wilt-resistant Pinus densiflora superior families for tissue culture propagation[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2013, 37(02): 50-54 https://doi.org/10.3969/j.issn.1000-2006.2013.02.009

中图分类号： S722.3+7 S763

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基金

收稿日期:2012-05-03 修回日期:2012-11-02
基金项目:国家林业局“948”项目(2011-4-69); 国家林业公益性行业科研专项项目(201204501); 江苏高校优势学科建设工程资助项目(PAPD)
第一作者:李清清,硕士生。*通信作者:叶建仁,教授。E-mail: jrye@njfu.com.cn。
引文格式:李清清,叶建仁,吴小芹. 抗松材线虫赤松组培繁殖优良家系的筛选[J]. 南京林业大学学报:自然科学版,2013,37(2):50-54.