抗松材线虫赤松组培繁殖优良家系的筛选

李清清,叶建仁,吴小芹

南京林业大学学报(自然科学版) ›› 2013, Vol. 37 ›› Issue (02) : 50-54.

PDF(1825609 KB)
PDF(1825609 KB)
南京林业大学学报(自然科学版) ›› 2013, Vol. 37 ›› Issue (02) : 50-54. DOI: 10.3969/j.issn.1000-2006.2013.02.009
研究论文

抗松材线虫赤松组培繁殖优良家系的筛选

  • 李清清,叶建仁*,吴小芹
作者信息 +

Selection of wilt-resistant Pinus densiflora superior families for tissue culture propagation

  • LI Qingqing,YE Jianren*, WU Xiaoqin
Author information +
文章历史 +

摘要

以抗松材线虫赤松(简称抗病赤松)27个家系带子叶顶芽的胚苗为外植体,筛选生长势好、增殖系数高和生根能力强的组培繁殖优良家系。结果表明:1、8、10、13、22和25家系的平均伸长率都达到了100%以上; 随着增殖代数的增加,各家系丛生芽增殖系数表现不一致,但总体趋势是l至2代丛生芽增殖系数随代数增加而增加,2至3代增殖系数随代数增加而下降; 抗病赤松不同无性系不定根诱导率不同,19-B无性系的不定根诱导率最高为95.8%。

Abstract

Using the embryo seedlings with cotyledons of 27 wilt-resistant Pinus densiflora families as explants, superior families which were good growth vigor, high multiplication rate and strong rooting ability were selected. The average elongation percentages of No.1, 8, 10, 13, 22 and 25 families were up to above 100%; With the increase of proliferation generations, the multiplication coefficient of clustered shoots in each family was variable, but the overall trend was that multiplication coefficient of clustered shoots from generation 1 to generation 2 increased with generation increase, and multiplication coefficient from generation 2 to generation 3 decreased; Adventitious root induction rate of wilt-resistant P. densiflora clones was different, 19-B clone of wilt-resistant P. densiflora was the highest,up to 95.8%.

引用本文

导出引用
李清清,叶建仁,吴小芹. 抗松材线虫赤松组培繁殖优良家系的筛选[J]. 南京林业大学学报(自然科学版). 2013, 37(02): 50-54 https://doi.org/10.3969/j.issn.1000-2006.2013.02.009
LI Qingqing,YE Jianren, WU Xiaoqin. Selection of wilt-resistant Pinus densiflora superior families for tissue culture propagation[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2013, 37(02): 50-54 https://doi.org/10.3969/j.issn.1000-2006.2013.02.009
中图分类号: S722.3+7    S763   

参考文献

[1] Sommer H E, Brown C L, Kormanik P P. Differentiation of plantlets in longleaf pine(Pinus palustris Mill.)tissue cultured in vitro [J].Bot Gaz,1975,136(2):196-200.
[2] Choi M S, Lee S G, Park Y G, et al. Micropropagation of Pinus densiflora for. erecta Uyeki[J]. Korean Journal of Plant Tissue Culture, 1993, 20(6): 315-320.
[3] 朱丽华,吴小芹,戴培培,等.赤松离体培养植株再生体系的建立[J].南京林业大学学报:自然科学版,2010,34(2):11-14. Zhu L H,Wu X Q,Dai P P, et al. In vitro plantlet regeneration from seedling explants of Pinus densiflora[J]. Journal of Nanjing Forestry University:Natural Sciences Edition, 2010,34(2):11-14.
[4] 秦莉,吴小芹,王张丽.抗松材线虫病赤松组培苗的生根与移栽研究[J].南京林业大学学报: 自然科学版,2011,35(3):89-92. Qin Li,Wu X Q,Wang Z L. Study on rooting and transplanting of the tissue-cultured plantlets from Pinus densiflora with good resistance to pine wilt disease[J]. Journal of Nanjing Forestry University: Natural Sciences Edition, 2011,35(3):89-92.
[5] 秦莉,吴小芹,朱丽华,等.抗松材线虫病赤松组培再生植株田间生长表现[J].林业科技开发,2011,25(2):28-30. Qin L,Wu X Q,Zhu L H, et al. Growth performance on regenerated plantlets of Pinus densiflora resistance to pine wilt disease[J]. Forestry Science and Technology, 2011,25(2):28-30.
[6] 朱丽华,张艺,吴小芹. 湿地松的组织培养及植株再生[J].南京林业大学学报:自然科学版,2004,28(6):47-51. Zhu L H,Zhang Y,Wu X Q.Tissue culture and plant regeneration of Pinus elliottii[J]. Journal of Nanjing Forestry University:Natural Sciences Edition, 2004,28(6):47-51.
[7] 成小飞,花晓梅,李文钿.马尾松离体培养条件下的微繁殖和菌根的形成[J].林业科学研究,1995,8(3):241-246. Cheng X F,Hua X M, Li W D. Micropropagation and Mycorrhizae formation of Pinus massoniana Lamb.in vitro[J].Forest Research, 1995,8(3):241-246.
[8] 徐田青,吴丽君,赖钟雄. 湿地松不定芽增殖培养试验[J]. 福建林业科技,2009,36(3):60-63. Xu T Q, Wu L J, Lai Z X. Proliferation culture experiment of adventitious bud of Pinus elliottii[J]. Journal of Fujian Foresty Science and Technology, 2009,36(3):60-63.
[9] 黄学林,李筱菊. 高等植物组织离体培养的形态建成及其调控[M].北京:科学出版社,1995. Huang X L, Li X J. Morphogenesis and Regulation of Higher Plant Tissue Culture in vitro[M].Beijing:Science Press,1995.
[10] 王力华,贺凤美,邓正正,等.日本落叶松微体繁殖及植株再生[J].东北林业大学学报,2004,32(6):19-23. Wang L H, He F M, Deng Z Z,et al.The Micropropagation from axillary bud and seedlings regeneration of Larix kaempferi[J].Journal of Northeast Forestry University, 2004,32(6):19-23.
[11] 黄宝祥,符树根,舒惠理.湿地松丛生芽的增殖研究[J].现代农业科技,2007(13):13-14. Huang B X, Fu S G,Shu H L. Studies on proliferation of axillary buds of Pinus elliottii[J]. Modern Agricultural Science and Technology, 2007(13):13-14.
[12] Bergmann B A, Stomp A M. Effect of genotype on in vitro adventitious shoot formation in Pinus radiate and correlations between pairs of phenotypic traits during in vitro shoot development[J]. Plant Cell, Tissue and Organ Culture 1994,39:185-194.
[13] 吴丽君,叶建仁,王志洁,等.湿地松组培继代增殖的影响因子[J].福建林学院学报,2007,27(2):165-169. Wu L J, Ye J R, Wang Z J,et al.The factors affecting multiplication rate in vitro culture of slash pine[J].Journal of Fujian College of Forestry, 2007,27(2):165-169.
[14] 吴大忠.不同杉木无性系组培增殖效果的比较研究[J]. 安徽农学通报,2007,13(17):113-114. Wu D Z.Comparative research on proliferation effect of different Chinese fir clones in tissue culture[J].Anhui Agri Sci Bull, 2007,13(17): 113-114.
[15] 吴丽君.湿地松组培快繁优良基因型的筛选[J].福建农业学报,2009,24(2):167-170. Wu L J. Selection of slash pine elite genotype for tissue culture and fast propagation[J].Fujian Journal of Agricultural Sciences, 2009,24(2):167-170.
[16] 何松林,孔德政,杨秋生,等.组织培养容器环境因子调控技术研究进展[J].河南农业大学学报,2003,37(1):25-32. He S L, Kong D Z,Yang Q S,et al. Research advance of environment factors’controlling technique in tissue culture vessel[J].Journal of Henan Agricultural University, 2003,37(1):25-32.
[17] Cuesta C, Ordas R J, Fernandez B,et al. Clonal micropropagation of six selected half-sibling families of Pinus pinea and somaclonal variation analysis[J]. Plant Cell,Tissue Organ Culture,2008,95(1):125-130.
[18] 欧阳磊. 邓恩桉组培生根影响因子的研究[J].福建林学院学报,2006,26(1):78-82. Ou Y L. Studies on effects of different factors on Eucalyptus dunnii in vitro culture[J].Journal of Fujian College of Forestry, 2006,26(1):78-82.
[19] 张艺,吴小芹,王钰,等.抗松材线虫病赤松家系的抗性测定及组织病理学观察[J].南京林业大学学报:自然科学版,2007,31(4):110-114. Zhang Y, Wu X Q, Wang Y,et al. Resistance determination and histopathological observation of induced Pinus densiflora families from Japan to Bursaphelenchus xylophilus[J]. Journal of Nanjing Forestry University:Natural Sciences Edition, 2007,31(4):110-114.

基金

收稿日期:2012-05-03 修回日期:2012-11-02
基金项目:国家林业局“948”项目(2011-4-69); 国家林业公益性行业科研专项项目(201204501); 江苏高校优势学科建设工程资助项目(PAPD)
第一作者:李清清,硕士生。*通信作者:叶建仁,教授。E-mail: jrye@njfu.com.cn。
引文格式:李清清,叶建仁,吴小芹. 抗松材线虫赤松组培繁殖优良家系的筛选[J]. 南京林业大学学报:自然科学版,2013,37(2):50-54.

PDF(1825609 KB)

Accesses

Citation

Detail

段落导航
相关文章

/