模拟野外漫灌洗盐并控制洗盐次数得到不同含盐量的土壤,比较单纯生物刺激、生物刺激加生物强化处理对不同含盐量的石油污染土壤的修复效果。结果表明:无论是单纯的生物刺激,还是生物刺激加生物强化处理下,土壤含盐量均有一定程度的降低,可有效提高石油烃的降解率。当土壤含盐量为0.22%(质量分数)时,添加4%菌剂修复第3天时,石油烃降解率可达24.98%; 当土壤含盐量为0.01%时,生物刺激加生物强化处理28 d后石油烃的降解率是单纯生物强化的1.1倍; 低含盐量添加4%菌剂处理下土壤石油烃降解率是高含盐量加4%菌剂处理的1.22倍。各处理下土壤脱氢酶活性随着培养时间逐渐增强,pH则随着培养时间有所下降,土壤盐碱性得到改良; 土壤环境得到改善,微生物的种类及数量增加。
Abstract
This study attempts to analyze the effects of different concentrations of soil salinityon bioremediation of petroleum-contaminated soil using biostimulation appended bioaugmentation or not. The saline-alkali soil collected from Tianjin Dagang oilfield and leached several times by analog field irrigation. The results showed that both the bioremediationcould reduce the soil salt content and raise oil petroleum hydrocarbon degradation rate. When soil salt content on the degree of 0.22%, biostimulation appended bioaugmentation could raise oil degradation rate to 24.98% after 3 days remediation.When soil salt content was on the degree of 0.01%, after 28 days biostimulation, the degradation rate by using biostimulation appended bioaugmentation was 1.1 times higher than that using pure biostimulation. The degradation rate of the low salt content soil was 1.22 times higher than that of the high salt content soilunder biostimulation appended bioaugmentation treatment. As the incubation time extended, soil dehydrogenase activity gradually increased, while soil pH value declined and soil salinization improved. As soil environment improved, the species and amounts of microorganism increased.
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] 丁克强,骆永明,孙铁珩,等.通气对石油污染土壤生物修复的影响[J].土壤,2001,33(4):185-188. Ding K Q, Luo Y M,Sun T H. The effect of air on bioremediation of the soil contaminated by petroleum hydrocarbons[J]. Soil, 2001, 33(4):185-188.
[2] 韩慧龙,汤晶,江皓,等.真菌-细菌修复石油污染土壤的协同作用机制研究[J].环境科学,2008,29(1):189-195. Han H L,Tang J,Jiang H, et al. Synergy between fungi and bacteria in fungi-bacteria augmented remediation of petroleum-contaminated soil[J]. Environmental Science, 2008, 29(1):189-195.
[3] 姚德明,许华夏,张海荣,等.石油污染土壤生物修复过程中微生物生态研究[J].生态学杂志,2002,21(1):26-28. Yao D M, Xu H X, Zhang H R, et al. Microbiological ecology during bioremediation for oil contaminated soil[J]. Chinese Journal of Ecology, 2002, 21(1):26-28.
[4] 刘五星,骆永明,滕应,等.石油污染土壤的生物修复研究进展[J].土壤,2006,38(5):634-639. Liu W X, Luo Y M, Teng Y, et al. Advances in bioremediation of petroleum contaminated soil[J]. Soils, 2006, 38(5):634-639.
[5] Ayotamuno M J, Kogbara R B, Ogaji S O T,et al. Bioremediation of a crude-oil polluted agricultural-soil at Port Harcour,Nigeria[J]. Applied Energy,2006,83(11):1249-1257.
[6] 叶淑红,丁鸣,马达,等.微生物修复辽东湾油污染湿地研究[J].环境科学,2005,26(5):143-146. Ye S H, Ding M, Ma D, et al. Research of microbio-remediation of oil-contaminated wetland in Liaodong Bay[J]. Environmental Science, 2005,26(5):143-146.
[7] 张宝良.油田土壤石油污染与原位生物修复技术研究[D].大庆:大庆石油学院,2007. Zhang B L. Research on in-situ bioremediation for oil-contaminated soil in oilfields[D]. Daqing: Daqing Petroleum Institute, 2006.
[8] 刘光崧.土壤理化分析与剖面描述[M].北京:中国标准出版社,1997. Liu G S. Soil Physical and Chemical Analysis & Description of Soil Profiles[M]. Beijing: Standards Press of China,1996.
[9] 王如刚,王敏,牛晓伟,等.超声-索氏萃取-重量法测定土壤中总石油烃含量[J].分析化学,2010,38(3):417-420. Wang R G, Wang M, Niu X W, et al. Determination of total petroleum hydrocarbons content in soil by ultrasonic-soxhlet extraction-gravimetric analysis[J].Chinese Journal of Analytical Chemistry, 2010, 38(3):417-420.
[10] 张清敏.环境生物学实验技术[M].北京:化学工业出版社,2005. Zhang Q M. Environmental Biology Experiment Technology[M]. Beijing: Chemical Industry Publishers, 2005.
[11] Muyzer G, de Waal E C, Uitterlinden A G. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA[J]. Applied and Environmental Microbiology, 1993, 59(3):695-700.
[12] 顿咪娜,胡文容,裴海燕,等.脱氢酶活性检测方法及应用[J].工业水处理,2008,28(10):1-4. Dun M N, Hu W R, Pei H Y, et al. Determination of dehydrogenase activity and its application[J]. Industrial Water Treatment, 2008,28(10):1-4.
[13] 解军,祁峰,裴海燕,等.脱氢酶活性检测方法及其在环境监测中的应用[J].中国环境监测,2006,22(5):13-18. Xie J, Qi F, Pei H Y, et al. Determining method of dehydrogenase activity and its application in environmental monitoring[J]. Environmental Monitoring in China,2006,22(5):13-18.
[14] Neilson A H. Organic Chemicals: An Environmental Perspective[M]. New York: Lewis Publishers, 2000.
[15] 牛志卿,刘建荣,吴国庆.TTC—脱氢酶活性测定法的改进[J].微生物学通报,1994,21(1):59-61. Niu Z Q, Liu J R, Wu G Q. TTC-the improvement of a dehydrogenase activity determination method[J]. Journal of Microbiology, 1994,21(1):59-61.
基金
收稿日期:2011-06-21 修回日期:2012-02-22
基金项目:环境污染过程与基准教育部重点实验室开放基金项目; 国家高技术研究发展计划(2007AA061201); 中国科学院知识创新工程项目(kzcx1-yw-06-03)
第一作者:秦晓,硕士生。*通信作者:李德生,教授。E-mail: deshli@tjut.edu.cn。
引文格式:秦晓,李德生,唐景春,等. 含盐量对石油污染土壤生物修复的影响[J]. 南京林业大学学报:自然科学版,2012,37(2):
PDF(1049622 KB)
