毛杨梅树皮原花色素对小鼠急性毒性及抗氧化性的影响

张伦; 刘功骏; 何柏球; 王飞

doi:10.3969/j.issn.1000-2006.2013.04.016

张伦,刘功骏,何柏球,王飞

南京林业大学学报（自然科学版） ›› 2013, Vol. 37 ›› Issue (04) : 85-89.

PDF(803438 KB)

国家林草科技领军期刊
中国精品科技期刊
中国高校百佳科技期刊
江苏省新闻出版政府奖期刊奖
RCCSE林学权威期刊（A+）
CSCD核心期刊
Scopus数据库收录期刊
中文核心期刊
SCD核心期刊

作者加群：102861116

微信公众号：南京林业大学学报

高级检索

PDF(803438 KB)

南京林业大学学报（自然科学版） ›› 2013, Vol. 37 ›› Issue (04) : 85-89. DOI: 10.3969/j.issn.1000-2006.2013.04.016

研究论文

毛杨梅树皮原花色素对小鼠急性毒性及抗氧化性的影响

张伦,刘功骏,何柏球,王飞

作者信息 +

Acute toxicity of proanthocyanidins from Myrica esculenta Buch. Ham. bark on mice and its antioxidative properties

ZHANG Lun, LIU Gongjun, HE Baiqiu, WANG Fei

Author information +

文章历史 +

摘要

探讨了毛杨梅树皮原花色素提取物的安全性,分析了毛杨梅树皮原花色素对小鼠的急性毒性以及在小鼠体内的抗氧化性能。结果表明:毛杨梅树皮原花色素提取物经口的LD₅₀为5 g/kg。毛杨梅树皮原花色素给药后,小鼠血清内的过氧化氢酶(CAT)、超氧化物歧化酶(SOD)活性显著性增加(p<0.05),丙二醛(MDA)含量减少。小鼠肝内的CAT活力显著增加,MDA含量也有所增加,但是并没有显著性差异(p>0.05)。因此,毛杨梅树皮原花色素属于实际无毒物质,毛杨梅树皮原花色素提取物对小鼠机体具有一定的抗氧化作用。

Abstract

The security of proanthocyanidins extractive from Myrica esculenta Buch. Ham. was investigated by analyzing its acute toxicity and antioxidative properties in vivo to mice. The results showed that the LD₅₀ of proanthocyanidins extractive from M. esculenta Buch. Ham. bark in mice was 5 g/kg, and the activities of catalase(CAT), superoxide dismutase(SOD)significant increased(p<0.05); the contents of malondialdehyde(MDA)decreased in mice serum. In liver, the activities of CAT increased(p<0.05), the content of MDA increased but was not significant difference with control(p>0.05). Thus, during the acute oral toxicity, the extractive from M. esculenta Buch. Ham. bark did not reveal any toxic effects in ICR mice(data not shown)and have some antioxidative properties in vivo.

引用本文

EndNote

Ris (Procite)

Bibtex

导出引用

张伦,刘功骏,何柏球,王飞. 毛杨梅树皮原花色素对小鼠急性毒性及抗氧化性的影响[J]. 南京林业大学学报（自然科学版）. 2013, 37(04): 85-89 https://doi.org/10.3969/j.issn.1000-2006.2013.04.016

ZHANG Lun, LIU Gongjun, HE Baiqiu, WANG Fei. Acute toxicity of proanthocyanidins from Myrica esculenta Buch. Ham. bark on mice and its antioxidative properties[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2013, 37(04): 85-89 https://doi.org/10.3969/j.issn.1000-2006.2013.04.016

中图分类号： S718.4 TQ91

参考文献

[1] 温鹏飞,陈建业,黄卫东.原花色素研究进展[J].农业工程学报, 2004,20(z1): 25-29 Wen P F, Chen J Y, Huang W D. Research advance of proanthocyanidins study[J]. Transactions of the CSAE, 2004,20(z1): 25-29.
[2] 肖红梅,孟凡华,王光霞.番茄不同组织原花色素含量研究[J].内蒙古大学学报:自然科学版,2009,40(3):352-355. Xiao H M, Meng F H, Wang G X. A study on the content of oligomeric proanthocyanidins in different organizations of tomato plants[J]. Journal of Inner Mongolia University:Natural Sciences Edition, 2009,40(3):352-355.
[3] 陈笳鸿,汪泳梅,吴冬梅.毛杨梅树皮提取物抗氧化及清除自由基活性初步研究[J].林产化学与工业,2007,10(27):1-7. Chen J H, Wang Y M, Wu D M. Preliminary study on antioxidative and radical-scavenging activities of extracts from Myrica esculenta Buch.-Ham. bark[J]. Chemistry and Industry of Forest Products, 2007,10(27):1-7.
[4] 黄初升,白素平,李英. 天然线型二芳基庚烷类化合物[J].天然产物研究与开发, 1997, 9(2): 98-105. Huang C S, Bai S P, Li Y. Naturally occurring linear diarylheptanoids[J]. Natural Product Research and Development, 1997, 9(2): 98-105.
[5] 唐霖,张莉静,王明谦.杨梅中活性成分杨梅素的研究进展[J]. 中成药, 2006,28(1):121-122. Tang L, Zhang L J,Wang M Q. Research advance of myricetin in Myrica active ingredient[J]. Chinese Traditional Patent Medicine, 2006,28(1):121-122.
[6] Matsuda H, Yamazaki M, Matsuo K, et al. Anti-androgenic activity of Myrica cortex-isolation of active constituents from bark of myrica rubra[J]. Biol Pharm, 2001,24:259-263.
[7] Supriya A, Sharad W M Ali. Essential oil of Myrica esculenta Buch. Ham.: composition, antimicrobial and topical anti-inflammatory activities[J]. Natural Product Research, 2012,1: 1-4
[8] Zdunczyk Z, Frejnagel S, Wroblewska M. Biological activity of polyphenol extracts from different plant sources[J]. Food Research International, 2002,35:183-186.
[9] Toshiaki A. The antioxidative function, preventive action on disease and utilization of proanthocyanidins[J]. Bio Factors, 2004, 21:197-201
[10] 黄雨三.保健食品检验与评价技术规范[M]. 北京:清华同方电子出版社,2003.
[11] 王苏斌. SPSS统计分析[M]. 北京:机械工业出版社, 2003.
[12] 谭毓治,伍爱婵,万晓霞,等. 葡萄籽原花青素对学习记忆的影响和急性毒性[G]∥中国药理学会.黄山:中药药理与中药现代化暨钙研讨会论文汇编, 2003.
[13] 谢文利,孙静,赵艳威,等.原花青素的降血糖作用及急性毒性研究[J].中草药, 2009,40(10):1615-1616. Xie W L, Sun J, Zhao Y W, et al. Study on hypoglycemic effect and acute toxicity of proanthocyanidins[J]. Chinese Traditional and Herbal Drugs, 2009,40(10):1615-1616.
[14] 黄永洪,花慧,沈国强,等.猪肝过氧化氢酶提取条件的研究[J].生物技术通讯,2005,16(1):40-42. Huang Y H, Hua H, Shen G Q, et al. Study on extraction of catalase from pig liver[J]. Letters in Biotechnology, 2005,16(1):40-42.
[15] 陈金峰,程素满.过氧化氢酶在衰老和疾病调控中的作用机制[J].医学分子生物学杂志, 2007,4(3): 276-278. Chen J F, Cheng S M. Effect of catalase on senescence and diseases[J]. Journal of Medical Molecular Biology, 2007,4(3): 276-278.
[16] Halliwell B, Gutteridge J M C. Free radicals in biology and medicine[M]. Oxford: Clarendon Press, 1989.
[17] Leeuwenburgh C, Ji L L. Glutathione depletion in rested and exercised mice: biochemical consequence and adaptation[J]. Arch Biochem Biophys, 1995(16): 941-949.
[18] Lenaz G. Role of mitochondria in oxidative stress and aging[J]. Biochim Biophys Acta,1998, 1366: 53-67.
[19] 张丁,张杰,孟光,等. 低聚原花青素及银杏黄酮对小鼠体内脂质过氧化和延缓果蝇衰老试验研究[J]. 河南预防医学杂志, 2002,13(5):257-260. Zhang D, Zhang J, Meng G, et al. Ezperment study on oligonerie proanthocyanidins and ginko flavonides to the mice lipid peroxides and postponing fly aging[J]. Henan Journal of Preventive Medicine, 2002,13(5):257-260.
[20] 陈永芬,申琳,鱼泳泳,等. 低聚原花青素方剂对小鼠肝脏、血清中相关抗氧化酶活性及MDA、活性氧含量的影响[J].食品科学, 2004,25(S1): 162-165. Chen Y F,Shen L,Yu Y Y, et al. The effect of oligomeric proanthocyanidins formula to antioxidase, MDA, reactive oxygen species in the mice liver and serum[J]. Food Science, 2004,25(S1): 162-165.

基金

收稿日期:2012-06-04 修回日期:2012-11-05
基金项目:国家林业公益性行业科研专项项目(201104019); 江苏高校优势学科建设工程项目(PAPD)
第一作者:张伦,博士生。*通信作者:王飞,教授,博士。E-mail: hgwf@njfu.edu.cn。
引文格式:张伦,刘功骏,何柏球,等. 毛杨梅树皮原花色素对小鼠急性毒性及抗氧化性的影响[J]. 南京林业大学学报:自然科学版,2013,37(4):85-89.