杉木成年优树组培生根研究

doi:10.3969/j.issn.1000-2006.2013.06.034

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南京林业大学学报（自然科学版） ›› 2013, Vol. 37 ›› Issue (06): 169-172.doi: 10.3969/j.issn.1000-2006.2013.06.034

杉木成年优树组培生根研究

周小红¹,张元莉¹, 李美平¹,赵芳芳¹,陈金慧¹,郑仁华²,肖晖²,黄金华³,张志才³,李勇³,郑雪燕³,施季森^1*

1.南京林业大学,林木遗传与生物技术省部共建教育部重点实验室,江苏南京 210037;
2.福建省林业科学研究院,福建福州 350000;
3.福建省洋口国有林场,福建顺昌 353211

出版日期:2013-12-18 发布日期:2013-12-18
基金资助:
收稿日期:2012-07-26 修回日期:2013-09-09
基金项目:国家林业公益性行业专项项目(201004049); 福建省林木种苗科技攻关项目(闽林科【2009】4号); 国家自然科学基金重点项目(30930077); 江苏高校优势学科建设工程资助项目(PAPD); 江苏省高校研究生科研创新计划资助项目(CXZZ11_0533)
第一作者:周小红,博士生。*通信作者:施季森,教授。E-mail: jshi@njfu.edu.cn。
引文格式:周小红,张元莉, 李美平,等. 杉木成年优树组培生根研究[J]. 南京林业大学学报:自然科学版,2013,37(6):169-172.

Studies on factors influencing rooting efficiency from mature Chinese fir superior clones in vitro

ZHOU Xiaohong¹, ZHANG Yuanli¹, LI Meiping¹, ZHAO Fangfang¹, CHEN Jinhui¹, ZHENG Renhua², XIAO Hui², HUANG Jinhua³, ZHANG Zhicai³, LI Yong³, ZHEN

1. Key Laboratory of Forest Genetics and Biotechnology Ministry of Education, Nanjing Forestry University, Nanjing 210037, China;
2. Fujian Academy of Forestry Science, Fuzhou 350000, China;
3. Yangkou National Forest Farms, Shunchang 353211, C

Online:2013-12-18 Published:2013-12-18

摘要/Abstract

摘要： 以3个成年杉木优良无性系茎段为外植体,研究基因型、生长调节剂、活性炭、基本培养基对杉木生根效率的影响。结果表明:基因型和生长素NAA是影响杉木生根效率的主要因素。1#基因型的杉木生根能力强,在1/4 DCR培养基(添加IBA 1.0 mg/L或NAA 0.5～1.0 mg/L)上生根率可达90%以上; 2#基因型的杉木生根能力较弱,添加1.5 mg/L IBA或1.0 mg/L NAA 的1/2 DCR 培养基更适合其生根; 对于3#基因型的杉木,3/4 DCR培养基最适合其生根,添加IBA 1.5 mg/L或NAA1.0 mg/L时生根率最高。对3个基因型的生根试验研究表明,活性炭对杉木生根不能起到促进作用。

Abstract: Using three different genotypes of shoot sections of mature superior clones of Cunninghamia lanceolata, the effect of factors such as plant growth regulators(PGRs), genotypes, basal medium and activated charcoal were studied for rooting frequency. The results showed that genotypes and NAA were key factors influencing rooting efficiency. Genotype 1# was more capable of rooting. The basal medium 1/4 DCR added with IBA 1.0 mg/L or NAA 0.5-1.0 mg/L could increase rooting efficiency to the level of 90%. Genotype 2# had a weaker ability of rooting. The 1/2DCR basal medium added with IBA 1.5 mg/L or NAA 1.0 mg/L was better in adventitious roots induction for this genotype. For genotype 3#, it had the best rooting efficiency when the level of IBA was 1.5 mg/L or the level of NAA was 1.0 mg/L, 3/4 DCR was fit for rooting. Addition of activated charcoal could not effectively improve rooting in this experiment.

中图分类号:

Q813.1⁺2

周小红,张元莉,李美平,等. 杉木成年优树组培生根研究[J]. 南京林业大学学报（自然科学版）, 2013, 37(06): 169-172.

ZHOU Xiaohong, ZHANG Yuanli, LI Meiping, ZHAO Fangfang, CHEN Jinhui, ZHENG Renhua, XIAO Hui, HUANG Jinhua, ZHANG Zhicai, LI Yong, ZHEN. Studies on factors influencing rooting efficiency from mature Chinese fir superior clones in vitro[J].Journal of Nanjing Forestry University (Natural Science Edition), 2013, 37(06): 169-172.DOI: 10.3969/j.issn.1000-2006.2013.06.034.

参考文献

[1] 雷泽勇. 针叶树无性繁殖研究进展[J]. 防护林科技, 2001, 1(1):55-57.Lei Z Y. Advance of research on agamogenesis of coniferous trees[J]. Protection Forest Science and Technology, 2001, 1(1):55-57.
[2] 俞新妥. 我国杉木资源动态及对营林的几点建议[J]. 热带林业, 1986(3):18-22. Yu X T. Recourse dynamic and suggestion for forest culture and management on Chinese fir in China[J]. Tropical Forestry,1986(3):18-22.
[3] 阙国宁. 杉木组培嫩梢增殖与复壮的分析[J]. 林业科学研究, 1989,2(6):54-55.Que G N. Analysis of buds’ proliferation and rejuvenation on Chinese fir[J]. Forest Research, 1989,2(6):54-55.
[4] 苏秀城. 杉木无性系不同继代数组培苗差异研究[J]. 福建林学院学报, 2000, 20(4):353-356.Su X C. Study on the differences of the seedling of different generation from successive tissue culture[J]. Journal of Fujian College of Forestry, 2000, 20(4):353-356.
[5] 席梦利, 施季森. 杉木子叶和下胚轴的器官发生与体胚发生[J]. 分子植物育种, 2005, 3(6):846-852.Xi M L, Shi J S. Organogenesis and somatic embryogenesis from cotyledons and hypocotyls of Cunninghamia lanceolata Hook. [J]. Molecular Plant Breeding, 2005, 3(6):846-852.
[6] 陈勤, 王斐, 琚淑明. 杉科植物组培生根研究机理综述[J]. 林业实用技术, 2011(5):3-4.Chen Q, Wang F, Ju S M. Review of research on mechanism of rooting in vitro culture on Taxodiaceae[J]. Practical Forestry Technology, 2011(5):3-4.
[7] 黄碧华. 速生优良杉木组培快繁技术试验[J].福建林业科技, 2007, 34(2):133-136.Huang B H. Studies on rapid propagation of tissue culture on excellent fast-growing Chinese fir[J]. Journal of Fujian Forestry Sci and Tech, 2007, 34(2):133-136.
[8] 林景泉. 速生优良杉木组培继代及生根培养研究[J]. 安徽农业科学, 2011, 39(15):8933-8934.Lin J Q. Studies on subculture and rooting culture of superior C. lanceolata[J]. Journal of Anhui Agri Sci, 2011, 39(15):8933-8934.
[9] 欧阳磊, 郑仁华, 翁玉榛, 等. 杉木优良无性系组培快繁技术体系的建立[J]. 南京林业大学学报:自然科学版, 2007, 31(3):23-25.Ouyang L, Zheng R H, Wen Y Z, et al. Establishment of technique system of tissue culture on Chinese fir superior clones[J]. Journal of Nanjing Forestry University:Natural Sciences Edition, 2007, 31(3):23-25.
[10] Gupta P K, Durzan D J. Shoot multiplication from mature trees of Douglas-fir(Pseudotsuga menziesii)and sugar pine(Pinus lambertiana)[J]. Plant Cell Reports, 1985, 4(4):177-179.
[11] 崔丽华.植物生长调节物质对组织培养中不定芽不定根的作用[J].辽宁师专学报,2000,2(2):97-99.Cui L H. The role of plant growth regulator for adventitious buds and roots in vitro culture[J]. Journal of Liaoning Teachers College, 2000, 2(2):97-99.
[12] Dumas E, Monteuuis O. In vitro rooting of micropropagated shoots from juvenile and mature Pinus pinaster explants: influence of activated charcoal[J]. Plant Cell, Tissue and Organ Culture, 1995, 40(3):231-235.
[13] Mantell S H, Hugo S A. Effects of photoperiod, mineral medium strength, inorganic ammonium, sucrose and cytokinin on root, shoot and microtuber development in shoot cultures of Dioscorea alata L. and D. bulbifera L. yams[J]. Plant Cell, Tissue and Organ Culture, 1989, 16(1): 23-37.
[14] Mishra R, Wang H Y, Yadav N R, et al. Development of a highly regenerable elite Acala cotton(Gossypium hirsutum cv. Maxxa)-a step towards genotype-independent regeneration[J]. Plant Cell, Tissue and Organ Culture, 2003, 73(1):21-35.

杉木成年优树组培生根研究

Studies on factors influencing rooting efficiency from mature Chinese fir superior clones in vitro

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