青钱柳愈伤组织不定芽诱导技术

阮氏钏; 方升佐; 尚旭岚; 杨万霞

doi:10.3969/j.issn.1000-2006.2014.02.011

青钱柳愈伤组织不定芽诱导技术

阮氏钏,方升佐,尚旭岚,杨万霞

南京林业大学学报（自然科学版） ›› 2014, Vol. 38 ›› Issue (02) : 52-56.

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南京林业大学学报（自然科学版） ›› 2014, Vol. 38 ›› Issue (02) : 52-56. DOI: 10.3969/j.issn.1000-2006.2014.02.011

研究论文

青钱柳愈伤组织不定芽诱导技术

阮氏钏^1,2,方升佐^1*,尚旭岚¹,杨万霞¹

作者信息 +

Induction techniques of adventitious shoot from callus of Cyclocarya paliurus in vitro

NGUYEN ThiXuyen^1,2,FANG Shengzuo^1*,SHANG Xulan¹,YANG Wanxia¹

Author information +

文章历史 +

摘要

以青钱柳成熟种子培养无菌苗,再用无菌苗叶和茎段为外植体进行组织培养,观察了愈伤组织形成及再分化形成不定芽的过程,分析了不同培养基和生长调节物质对青钱柳愈伤组织及不定芽发生的影响。结果表明:(1)MS、WPM和B5培养基均能诱导出愈伤组织,茎段和叶片的诱导率最高分别达到90.00%和86.70%;(2)激素及其浓度对愈伤组织诱导均有显著影响。当加入TDZ质量浓度为0.5 mg/L时,叶愈伤组织诱导率达95.00%; 而加入TDZ质量浓度为0. 1 mg/L时,茎段诱导率达92.85%;(3)叶愈伤组织在WPM+1.0 mg/L 6-BA+0.1 mg/L IBA的培养基上,不定芽的诱导频率最高,达53.85%,这实现了青钱柳由愈伤组织分化形成不定芽和萌发增殖的过程,但萌发率较低。

Abstract

Based on the seedlings cultured with matured seeds of Cyclocarya paliurus in vitro, the induction of adventitious shoot from callus of C. paliurus and the effects of basic media and different growth regulators on induction of callus and adventitious shoots were studied with explants of leaves and stem segments of the young seedlings. The results showed that callus of leaves and stem segments of the seedlings could be induced on each basic medium applied in this paper and the highest induction frequency of callus reached 90.00% for stem segment and 86.70% for leaf, respectively. The types and concentrations of hormones had significant effects on callus induction. When 0.5 mg/L TDZ was added, the induction frequency of leaf callus reached 95.00%, while 92.85% induction frequency was achieved for stem segment callus at 0.5 mg/L TDZ added. The greatest induction frequency of adventitious shoots from leaf callus was observed in the medium of WPM+1.0 mg/L 6-BA+0.1 mg/L IBA, which reached to 53.85%. Our results successfully achieved the induction and germination of adventitious shoots from the leaf callus, but the germination rate was still low and more studies should be done in the future.

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阮氏钏,方升佐,尚旭岚,杨万霞. 青钱柳愈伤组织不定芽诱导技术[J]. 南京林业大学学报（自然科学版）. 2014, 38(02): 52-56 https://doi.org/10.3969/j.issn.1000-2006.2014.02.011

NGUYEN ThiXuyen,FANG Shengzuo,SHANG Xulan,YANG Wanxia. Induction techniques of adventitious shoot from callus of Cyclocarya paliurus in vitro[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2014, 38(02): 52-56 https://doi.org/10.3969/j.issn.1000-2006.2014.02.011

中图分类号： S718

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基金

收稿日期:2013-05-01 修回日期:2013-07-04
基金项目:国家林业局林业科技成果推广项目([2011]18号); 国家林业公益性行业科研专项项目(20090406); 江苏高校优势学科建设工程资助项目(PAPD); 江苏高校协同创新计划
第一作者:阮氏钏,博士生。*通信作者:方升佐,教授。E-mail: fangsz@njfu.edu.cn。
引文格式:阮氏钏,方升佐,尚旭岚,等. 青钱柳愈伤组织不定芽诱导技术[J]. 南京林业大学学报:自然科学版,2014